The runaway black hole GRO J1655-40

We have used the Hubble Space Telescope to measure the motion in the sky and compute the galactocentric orbit of the black hole X-ray binary GRO J1655-40. The system moves with a runaway space velocity of $112\pm 18$ km s$^{-1}$ in a highly eccentric ($e = 0.34\pm 0.05$) orbit. The black hole was formed in the disk at a distance greater than 3 kpc from the Galactic centre and must have been shot to such an eccentric orbit by the explosion of the progenitor star. The runaway linear momentum and kinetic energy of this black hole binary are comparable to those of solitary neutron stars and millisecond pulsars. GRO J1655-40 is the first black hole for which there is evidence for a runaway motion imparted by a natal kick in a supernova explosion.Comment: Published in Astronomy and Astrophysics. 5 pages, 2 color figures. Color figure and animation can be found at http://www.iafe.uba.ar/astronomia/mirabel/mirabel.html or ftp://ftp.cea.fr/incoming/y2k01/mirabe

Puente de Troulero, Suiza

When it was decided by the local authorities and the Emosson, S. A., electricity firm to build an access road, it became necessary to construct a bridge at Troulero, to go over rocky fill material which covered a mountain side, in a limiting state of unstable equilibrium. The bridge is 140 m long, and rests on oblique reinforced and prestressed concrete supports. The completion of this project has taken three years, and it has cost over 620,000 Swiss Francs.AI ser decretada la construcción de una carretera de acceso por las autoridades cantonales y la dirección de electricidad de Emosson, S. A., fue necesaria la realización de una obra de fábrica —el puente de Troulero— que franquease unos echadizos rocosos en estado de equilibrio límite situados en una ladera. La obra construida, de 140 m de longitud, es un puente con soportes oblicuos de hormigón armado y pretensado. La duración de los trabajos ha sido de tres años, y el coste sobrepasa los 620.000 francos suizos

Migraine and cluster headache show impaired neurosteroids patterns

Background: Perturbation of neuronal excitability contributes to migraine. Neurosteroids modulate the activity of γ-aminobutyric acid A and N-methyl-d-aspartate receptors, and might be involved in the pathogenesis of migraine. Here, we measured plasma levels of four neurosteroids, i.e., allopregnanolone, epiallopregnanolone, dehydroepiandrosterone and deydroepiandrosterone sulfate, in patients affected by episodic migraine, chronic migraine, or cluster headache. Methods: Nineteen female patients affected by episodic migraine, 51 female patients affected by chronic migraine, and 18 male patients affected by cluster headache were recruited to the study. Sex- and age-matched healthy control subjects (31 females and 16 males) were also recruited. Patients were clinically characterized by using validated questionnaires. Plasma neurosteroid levels were measured by liquid chromatography-tandem mass spectrometry. Results: We found disease-specific changes in neurosteroid levels in our study groups. For example, allopregnanolone levels were significantly increased in episodic migraine and chronic migraine patients than in control subjects, whereas they were reduced in patients affected by cluster headache. Dehydroepiandrosterone and dehydroepiandrosterone sulfate levels were reduced in patients affected by chronic migraine, but did not change in patients affected by cluster headache. Conclusion: We have shown for the first time that large and disease-specific changes in circulating neurosteroid levels are associated with chronic headache disorders, raising the interesting possibility that fluctuations of neurosteroids at their site of action might shape the natural course of migraine and cluster headache. Whether the observed changes in neurosteroids are genetically determined or rather result from exposure to environmental or intrinsic stressors is unknown. This might also be matter for further investigation because stress is a known triggering factor for headache attacks in both migraineurs and cluster headache patients

New perspectives on an “old” technique: Lipari obsidian and Neolithic communities investigated by Fission Track Dating

“Lipari obsidian and Neolithic human communities in the Aeolian islands” is a project aimed at studying the connection between obsidian flows on the island of Lipari and Neolithic populations on the Aeolian archipelago, in Italy. As it is well known, obsidian is of particular interest to trace prehistorical trading patterns; indeed, Lipari obsidian has the widest distribution and has been found in southern France, Dalmazia, Sicily and mainland Italy. The project outputs will give a general vision of both archaeological and volcanological aspects through thestratigraphic and radiometric dating of eruptions which produced obsidian, in relationship with the first phases of the human settlements and row material exploitation. To reach this goal, we are considering both raw materials (geological samples) from different flows and artifacts from Neolithic settlements (archaeological samples) on the Aeolian islands, and performing fission track dating to get the age of obsidian sources and artefacts. Obtained results are expected to shed some new light on the raw material procurement and on the ability of the Neolithic populations to move from their locations, with particular attention to the consequences of environmental features on the first human settlements on the Aeolian islands

Enrichment of intestinal Lactobacillus by enhanced secretory IgA coating alters glucose homeostasis in P2rx7 −/− mice

The secretory immunoglobulin A (SIgA) in mammalian gut protects the organism from infections and contributes to host physiology by shaping microbiota composition. The mechanisms regulating the adaptive SIgA response towards gut microbes are poorly defined. Deletion of P2rx7, encoding for the ATP-gated ionotropic P2X7 receptor, leads to T follicular helper (Tfh) cells expansion in the Peyer\u2019s patches (PPs) of the small intestine, enhanced germinal centre (GC) reaction and IgA secretion; the resulting alterations of the gut microbiota in turn affects host metabolism. Here, we define gut microbiota modifications that correlate with deregulated SIgA secretion and metabolic alterations in P2rx7 12/ 12 mice. In particular, Lactobacillus shows enhanced SIgA coating in P2rx7 12/ 12 with respect to wild-type (WT) mice. The abundance of SIgA-coated lactobacilli positively correlates with Tfh cells number and body weight, suggesting Lactobacillus-specific SIgA response conditions host metabolism. Accordingly, oral administration of intestinal Lactobacillus isolates from P2rx7 12/ 12 mice to WT animals results in altered glucose homeostasis and fat deposition. Thus, enhanced SIgA production by P2X7 insufficiency promotes Lactobacillus colonization that interferes with systemic metabolic homeostasis. These data indicate that P2X7 receptor-mediated regulation of commensals coating by SIgA is important in tuning the selection of bacterial taxa, which condition host metabolism

First Measurement of the He3+He3-->He4+2p Cross Section down to the Lower Edge of the Solar Gamow Peak

We give the LUNA results on the cross section measurement of a key reaction of the proton-proton chain strongly affecting the calculated neutrino luminosity from the Sun: He3+He3-->He4+2p. Due to the cosmic ray suppression provided by the Gran Sasso underground laboratory it has been possible to measure the cross section down to the lower edge of the solar Gamow peak, i.e. as low as 16.5 keV centre of mass energy. The data clearly show the cross section increase due to the electron screening effect but they do not exhibit any evidence for a narrow resonance suggested to explain the observed solar neutrino flux.Comment: 5 pages, RevTeX, and 2 figures in PostScript Submitted for publicatio

The Tuberculosis Network European Trials group (TBnet) ERS Clinical Research Collaboration: addressing drug-resistant tuberculosis through European cooperation

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An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria

This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL2010 colonization in a murine model

"war to the knife" against thromboinflammation to protect endothelial function of COVID-19 patients

In this viewpoint, we summarize the relevance of thromboinflammation in COVID-19 and discuss potential mechanisms of endothelial injury as a key point for the development of lung and distant organ dysfunction, with a focus on direct viral infection and cytokine-mediated injury. Entanglement between inflammation and coagulation and resistance to heparin provide a rationale to consider other therapeutic approaches in order to preserve endothelial function and limit microthrombosis, especially in severe forms. These strategies include nebulized heparin, N-acetylcysteine, plasma exchange and/or fresh frozen plasma, plasma derivatives to increase the level of endogenous anticoagulants (tissue factor pathway inhibitor, activated protein C, thrombomodulin, antithrombin), dipyridamole, complement blockers, different types of stem cells, and extracellular vesicles. An integrated therapy including these drugs has the potential to improve outcomes in COVID-19