Optical and near-infrared observations of the GRB 970616 error box

We report on near-infrared and optical observations of the GRB 970616 error box and of the X-ray sources discovered by ASCA and ROSAT in the region. No optical transient was found either within the IPN band or in the X-ray error boxes, similarly to other bursts, and we suggest that either considerable intrinsic absorption was present (like GRB 970828) or that the optical transient displayed a very fast decline (like GRB 980326 and GRB 980519).Comment: 2 pages with one encapsulated PostScript figure included. Uses Astronomy & Astrophysics LaTeX macros. Accepted for publication in Astronomy & Astrophysics Supplement Serie

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.Fil: Chumbimuni Torres, Karin Y.. The University of Texas at San Antonio; Estados UnidosFil: Coronado, Ramon E.. The University of Texas at San Antonio; Estados UnidosFil: Mfuh, Adelphe M.. The University of Texas at San Antonio; Estados UnidosFil: Castro Guerrero, Carlos. The University of Texas at San Antonio; Estados UnidosFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Negrete, George R.. The University of Texas at San Antonio; Estados UnidosFil: Bizios, Rena. The University of Texas at San Antonio; Estados UnidosFil: Garcia, Carlos D.. The University of Texas at San Antonio; Estados Unido

Characterization and Di erentiation of Spanish Vinegars from Jerez and Condado de Huelva Protected Designations of Origin

Thirty one Jerez vinegar samples and 33 Huelva vinegar samples were analyzed for polyphenolic and volatile compound content in order to characterize them and attempt to di erentiate them. Sixteen polyphenolic compounds were quantified by means of ultraperformance liquid chromatography method with diode array detection (UPLC–DAD), and 37 volatile compounds were studied by means of stir bar sorptive extraction–gas chromatography–mass spectrometry (SBSE–GC–MS). Spectrophotometric CIELab parameters were also measured for all the samples. The results obtained from the statistical multivariate treatment of the data evidenced a clear di erence between vinegars from the two geographical indications with regard to their polyphenolic content, with Jerez vinegars exhibiting a greater phenolic content. Di erentiation by the volatile compound content was, however, not so evident. Nevertheless, a considerable di erentiation between the two groups of vinegars based on their volatile fraction was achieved. This may bring to light the grape varieties and geographical factors that have a clear influence on such di erences

Histologic features of bone regenerated by means of negative pressure in the context of odontogenic keratocyst

Propósito: El objetivo de la presente investigación es describir las características histológicas del hueso regenerado mediante presión negativa (sugosteogénesis) en un grupo de pacientes con diagnóstico de queratoquiste odontogénico (QO) sometidos a descompresión activa y sugosteogénesis por distracción (ADDS) en nuestra institución. Materiales y métodos: Los autores diseñaron un estudio retrospectivo de serie de casos. La población incluyó pacientes con diagnóstico histológico de queratoquiste odontogénico en los que se realizó descompresión activa y sugosteogénesis por distracción seguida de enucleación. Todos los pacientes fueron atendidos y seguidos desde julio de 2019 hasta enero de 2021. La investigación fue aprobada por la Junta de Revisión Institucional, y observó la Declaración de Helsinki sobre protocolo médico. Las variables de este estudio incluyeron la edad, el sexo, la localización anatómica (mandíbula o maxilar) y las características histológicas del hueso regenerado mediante presión negativa. Las características histológicas se definieron como consistentes o inconsistentes con hueso maduro viable. Resultados: Se consideraron las biopsias óseas de 6 pacientes. En total, el 83,33% de los pacientes eran varones y el 16,66% mujeres. El cien por cien de las muestras óseas sometidas a presión negativa mostraban características de hueso maduro viable. Conclusiones: En este estudio, las características histológicas del hueso sometido a presión negativa demostraron las características normales del hueso maduro normal. 2022, El/los autor/es, bajo licencia exclusiva de Springer-Verlag GmbH Alemania, parte de Springer Nature.Purpose: The objective of the present research is to describe the histologic features of the bone regenerated by means of negative pressure (sugosteogenesis) in a group of patients diagnosed with odontogenic keratocyst (OKC) who underwent active decompression and distraction sugosteogenesis (ADDS) at our institution. Materials and methods: The authors designed a retrospective case series study. The population included patients with a histologic diagnosis of odontogenic keratocyst in whom active decompression and distraction sugosteogenesis followed by enucleation was performed. All patients were seen and followed from July 2019 to January 2021. The investigation was approved by the Institutional Review Board, and it observed the Declaration of Helsinki on medical protocol. Variables of this study included age, gender, anatomic location (mandible or maxilla), and histologic characteristics of the bone regenerated by means of negative pressure. Histologic features were defined as being consistent or inconsistent with viable mature bone. Results: Bone biopsies of 6 patients were considered. In total, 83.33% of patients were males and 16.66% females. One hundred percent of the bone samples subjected to negative pressure showed features of viable mature bone. Conclusions: In this study, the histological features of the bone subjected to negative pressure demonstrated the normal characteristics of the mature, normal bone. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature

Eating quality of beef from biotypes included in the PGI “Ternera Asturiana” showing distinct physicochemical characteristics and tenderization pattern

determine if their differences in physicochemical characteristics and tenderization pattern during maturation (3 to 21 days) had an effect on the consumer evaluation of beef palatability. Biotype affected significantly pH, water holding capacity, chemical composition (Pb0.001) and meat lightness (Pb0.05). Ageing time affected significantly (Pb0.05) colour, meat toughness and sensory attributes in a different way within each biotype. Multivariate analysis showed two different meat groups: 1) meat from mh-genotypes, characterized by high juice losses, lightness (L*), protein content and high sensory acceptability at intermediate (7 and 14 days) ageing times; 2) meat from rustic (AM) breed and biotypes free of myostatin mutation (AV (+/+) and AV×AM), showing higher intramuscular fat, myoglobin content, and instrumental toughness and requiring longer storage times (21 days). This should be taken into account for the proper post-mortem management and commercialization of each product to achieve its best sensory quality

First approach to molecular epidemiology of bovine tuberculosis in Colombia

ABSTRACT Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.RESUMEN Objetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública

Design of alpha-S-Neoglycopeptides derived from MUC1 with a flexible and solvent-exposed sugar moiety

The use of vaccines based on MUC1 glycopeptides is a promising approach to treat cancer. We present herein several sulfa-Tn antigens incorporated in MUC1 sequences that possess a variable linker between the carbohydrate (GalNAc) and the peptide backbone. The main conformations of these molecules in solution have been evaluated by combining NMR experiments and molecular dynamics simulations. The linker plays a key role in the modulation of the conformation of these compounds at different levels, blocking a direct contact between the sugar moiety and the backbone, promoting a helix-like conformation for the glycosylated residue and favoring the proper presentation of the sugar unit for molecular recognition events. The feasibility of these novel compounds as mimics of MUC1 antigens has been validated by the X-ray diffraction structure of one of these unnatural derivatives complexed to an anti-MUC1 monoclonal antibody. These features, together with potential lack of immune suppression, render these unnatural glycopeptides promising candidates for designing alternative therapeutic vaccines against cancer

Intramolecular hydrogen-bond activation for the addition of nucleophilic imines: 2-hydroxybenzophenone as a chemical auxiliary

The addition of nucleophilic imines, using 2-hydroxybenzophenone as a chemical auxiliary, is presented. An intramolecular six-membered ring via hydrogen bonding that enhances the reactivity and selectivity is the key of this strategy, which is supported by DFT calculations and experimental trialsSpanish Government (CTQ2015-64561-R, CTQ2016-76061-P, and MDM-2014-0377), Prodep (UJAT-PTC-247) and CCC-UAM are acknowledged. A. G. thanks MINECO for a PhD fellowship (FPI) and A. M. S. thanks CAM for a postdoctoral contract (2016-T2/IND-1660