Violência doméstica em tempos de pandemia COVID-19 em Portugal

Tem errata da afiliaçãopublishersversionpublishersversionpublishe

Diet of two syntopic species of Crenuchidae (Ostariophysi: Characiformes) in an Amazonian rocky stream

Abstract This study assessed the diet of two poorly known syntopic fish species of the family Crenuchidae, Characidium aff. declivirostre and Leptocharacidium omospilus, in a Presidente Figueiredo´ rocky stream, Amazonas, Brazil. The stomach contents were analyzed and their Frequency of Occurrence (FO %) and Relative Volume (Vol %) were combined in a Feeding Index (IAi). We examined 20 individuals of C. aff. declivirostre and 23 of L. omospilus. The Morisita-Horn Index was used to estimate the overlap between the diets of these species. Immature insects were the most valuable items consumed by both fish species. The diet of C. aff. declivirostre was mainly composed of larvae and pupae of Chironomidae, while L. omospilus predominantly consumed larvae of Hydroptilidae, Hydropyschidae and Pyralidae. Thus, both species were classified as autochthonous insectivorous. Characidium aff. declivirostre was considered a more specialized species, probably reflecting lower feeding plasticity or the use of more restricted microhabitats compared to L. omospilus. When the food items were analyzed at the family taxonomic level, the diet overlap between these species was considered moderate (Morisita-Horn Index = 0.4). However, a more thorough analysis, at the genus level, indicates a very low diet overlap. Therefore, we conclude that the feeding segregation between C. aff. declivirostre and L. omospilus may favor their co-existence, despite their high phylogenetic closeness

“À flor” dos livros: a literatura na educação emocional infantil

Gerir as emoções é compreender e ser capaz de as viver de forma saudável e deve ser trabalhado desde os primeiros anos de vida. Nesse trabalho, família e escola devem desempenhar uma parceria ativa, no sentido de capacitar as crianças das ferramentas necessárias para a compreensão e gestão das suas emoções. Em nosso entender, e nesse âmbito, a literatura de potencial receção infantil tem um importante papel na formação da criança, não só concernente ao domínio cognitivo, mas também sócioafetivo. É da competência do adulto escolher as obras literárias que disponibiliza, sendo que os profissionais da educação têm um papel decisivo e devem ser conscientes da importância de uma boa escolha porque, um bom livro, mais que um objeto estético, é também um importante elemento no que concerne à educação emocional infantil. Respondendo às necessidades da Criança, além de facilitar o desenvolvimento da linguagem e da cognição, o livro (concretamente o álbum ilustrado) permite à Criança aumentar o conhecimento do mundo e estimula a sua criatividade e equilíbrio emocional. Com base num corpus textual selecionado e trabalhado na unidade curricular Literatura para a Infância, do 1.º ano do mestrado em Educação Pré-escolar e em Ensino do 1.º Ciclo do Ensino Básico, procuraremos evidenciar de que forma(s) a Literatura pode ser uma excelente maneira de formar crianças cada vez mais conscientes das suas próprias emoções e respeitadoras das dos outros.info:eu-repo/semantics/publishedVersio

Temperature Modulates the Secretome of the Phytopathogenic Fungus Lasiodiplodia theobromae

Environmental alterations modulate host-microorganism interactions. Little is known about how climate changes can trigger pathogenic features on symbiont or mutualistic microorganisms. Current climate models predict increased environmental temperatures.The exposing of phytopathogens to these changing conditions can have particularly relevant consequences for economically important species and for humans. The impact on pathogen/host interaction and the shift on their biogeographical range can induce different levels of virulence in new hosts, allowing massive losses in agricultural and health fields.Lasiodiplodia theobromae is a phytopathogenic fungus responsible for a number of diseases in various plants. It has also been described as an opportunist pathogen in humans, causing infections with different levels of severity. Lasiodiplodia theobromae has a high capacity of adaptation to different environments, such as woody plants, moist argillaceous soils or even humans, being able to grow and infect hosts in a wide range of temperatures (9ºC-39ºC). Nonetheless, the effect of an increase of temperature, as predicted in climate change models, on L. theobromae is unknown.Here we explore the effect of temperature on two strains of L. theobromae – an environmental strain CAA019, and a clinical strain, CBS339.90.We show that both strains are cytotoxic to mammalian cells but while the environmental strain is cytotoxic mainly at 25ºC, the clinical strain is cytotoxic mainly at 30ºC and 37ºC.Extracellular gelatinolytic, xylanolytic, amylolytic and cellulolytic activities at 25ºC and 37ºC were characterized by zymography and the secretome of both strains grown at 25ºC, 30ºC and 37ºC were characterized by electrophoresis and by Orbitrap LC-MS/MS. More than 75% of the proteins were identified, mostly enzymes (glycosyl hydrolases and proteases). The strains showed different protein profiles, which were affected by growth temperature. Also, strain specific proteins were identified, such as a putative f5/8 type c domain protein, known for being involved in pathogenesis, by strain CAA019 and a putative tripeptidyl-peptidase 1 protein, by strain CBS339.90.We showed that temperature modulates the secretome of L. theobromae. This modulation may be associated with host-specificity requirements. We show that the study of abiotic factors, such as temperature, is crucial to understand host/pathogen interactions and its impacts on disease