A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy

Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo

Novel theranostic nanoporphyrins for photodynamic diagnosis and trimodal therapy for bladder cancer

The overall prognosis of bladder cancer has not been improved over the last 30 years and therefore, there is a great medical need to develop novel diagnosis and therapy approaches for bladder cancer. We developed a multifunctional nanoporphyrin platform that was coated with a bladder cancer-specific ligand named PLZ4. PLZ4-nanoporphyrin (PNP) integrates photodynamic diagnosis, image-guided photodynamic therapy, photothermal therapy and targeted chemotherapy in a single procedure. PNPs are spherical, relatively small (around 23 nm), and have the ability to preferably emit fluorescence/heat/reactive oxygen species upon illumination with near infrared light. Doxorubicin (DOX) loaded PNPs possess slower drug release and dramatically longer systemic circulation time compared to free DOX. The fluorescence signal of PNPs efficiently and selectively increased in bladder cancer cells but not normal urothelial cells in vitro and in an orthotopic patient derived bladder cancer xenograft (PDX) models, indicating their great potential for photodynamic diagnosis. Photodynamic therapy with PNPs was significantly more potent than 5-aminolevulinic acid, and eliminated orthotopic PDX bladder cancers after intravesical treatment. Image-guided photodynamic and photothermal therapies synergized with targeted chemotherapy of DOX and significantly prolonged overall survival of mice carrying PDXs. In conclusion, this uniquely engineered targeting PNP selectively targeted tumor cells for photodynamic diagnosis, and served as effective triple-modality (photodynamic/photothermal/chemo) therapeutic agents against bladder cancers. This platform can be easily adapted to individualized medicine in a clinical setting and has tremendous potential to improve the management of bladder cancer in the clinic

Michel De Goedt, Le Christ de Thérèse de Jésus, 1993 Id., Le Christ de Jean de la Croix, 1993

Guelluy Robert. Michel De Goedt, Le Christ de Thérèse de Jésus, 1993 Id., Le Christ de Jean de la Croix, 1993. In: Revue théologique de Louvain, 26ᵉ année, fasc. 2, 1995. p. 258

Pierre Moitel, Je crois... Comment le dire ? (coll. Champs nouveaux), 1983

Guelluy Robert. Pierre Moitel, Je crois... Comment le dire ? (coll. Champs nouveaux), 1983. In: Revue théologique de Louvain, 17ᵉ année, fasc. 3, 1986. p. 377

G. Thibon, Le voile et le masque, 1985

Guelluy Robert. G. Thibon, Le voile et le masque, 1985. In: Revue théologique de Louvain, 17ᵉ année, fasc. 3, 1986. p. 375

Ronaldo Muñoz, Dieu : «J'ai vu la misère de mon peuple». Traduction de l'espagnol par Salomé de Unamuno. Présentation par P. M. Beaude (coll. Collection Libération). 1990

Guelluy Robert. Ronaldo Muñoz, Dieu : «J'ai vu la misère de mon peuple». Traduction de l'espagnol par Salomé de Unamuno. Présentation par P. M. Beaude (coll. Collection Libération). 1990. In: Revue théologique de Louvain, 22ᵉ année, fasc. 3, 1991. p. 433

Cardinal G. Danneels, L'humanité de Dieu. Entretiens avec Gwendoline Jarczyk, 1994

Guelluy Robert. Cardinal G. Danneels, L'humanité de Dieu. Entretiens avec Gwendoline Jarczyk, 1994. In: Revue théologique de Louvain, 26ᵉ année, fasc. 2, 1995. pp. 262-263

J. Javaux, Une affirmation raisonnée de Dieu (coll. Le point théologique, n. 7). 1973

Guelluy Robert. J. Javaux, Une affirmation raisonnée de Dieu (coll. Le point théologique, n. 7). 1973. In: Revue théologique de Louvain, 6ᵉ année, fasc. 2, 1975. p. 252

Athéisme et agnosticisme. Colloque de Bruxelles, mai 1986, 1986

Guelluy Robert. Athéisme et agnosticisme. Colloque de Bruxelles, mai 1986, 1986. In: Revue théologique de Louvain, 20ᵉ année, fasc. 1, 1989. pp. 101-103

Georges Tavard, Jean de la Croix, poète mystique., 1987

Guelluy Robert. Georges Tavard, Jean de la Croix, poète mystique., 1987. In: Revue théologique de Louvain, 19ᵉ année, fasc. 1, 1988. p. 109