Long-lived anomalous thermal diffusion induced by elastic cell membranes on nearby particles

The physical approach of a small particle (virus, medical drug) to the cell membrane represents the crucial first step before active internalization and is governed by thermal diffusion. Using a fully analytical theory we show that the stretching and bending of the elastic membrane by the approaching particle induces a memory in the system which leads to anomalous diffusion, even though the particle is immersed in a purely Newtonian liquid. For typical cell membranes the transient subdiffusive regime extends beyond 10 ms and can enhance residence times and possibly binding rates up to 50\%. Our analytical predictions are validated by numerical simulations.Comment: 13 pages and 5 figures. The Supporting Information is included. Manuscript accepted for publication in Phys. Rev.

Particle mobility between two planar elastic membranes: Brownian motion and membrane deformation

We study the motion of a solid particle immersed in a Newtonian fluid and confined between two parallel elastic membranes possessing shear and bending rigidity. The hydrodynamic mobility depends on the frequency of the particle motion due to the elastic energy stored in the membrane. Unlike the single-membrane case, a coupling between shearing and bending exists. The commonly used approximation of superposing two single-membrane contributions is found to give reasonable results only for motions in the parallel, but not in the perpendicular direction. We also compute analytically the membrane deformation resulting from the motion of the particle, showing that the presence of the second membrane reduces deformation. Using the fluctuation-dissipation theorem we compute the Brownian motion of the particle, finding a long-lasting subdiffusive regime at intermediate time scales. We finally assess the accuracy of the employed point-particle approximation via boundary-integral simulations for a truly extended particle. They are found to be in excellent agreement with the analytical predictions.Comment: 14 pages, 8 figures and 96 references. Revised version resubmitted to Phys. Fluid

Numerical-experimental observation of shape bistability of red blood cells flowing in a microchannel

Red blood cells flowing through capillaries assume a wide variety of different shapes owing to their high deformability. Predicting the realized shapes is a complex field as they are determined by the intricate interplay between the flow conditions and the membrane mechanics. In this work we construct the shape phase diagram of a single red blood cell with a physiological viscosity ratio flowing in a microchannel. We use both experimental in-vitro measurements as well as 3D numerical simulations to complement the respective other one. Numerically, we have easy control over the initial starting configuration and natural access to the full 3D shape. With this information we obtain the phase diagram as a function of initial position, starting shape and cell velocity. Experimentally, we measure the occurrence frequency of the different shapes as a function of the cell velocity to construct the experimental diagram which is in good agreement with the numerical observations. Two different major shapes are found, namely croissants and slippers. Notably, both shapes show coexistence at low (<1 mm/s) and high velocities (>3 mm/s) while in-between only croissants are stable. This pronounced bistability indicates that RBC shapes are not only determined by system parameters such as flow velocity or channel size, but also strongly depend on the initial conditions.Comment: 13 pages, 9 figures (main text). 13 pages, 31 figures (SI

Training high performance skills using above real-time training

The Above Real-Time Training (ARTT) concept is a unique approach to training high performance skills. ARTT refers to a training paradigm that places the operator in a simulated environment that functions at faster than normal time. Such a training paradigm represents a departure from the intuitive, but not often supported, feeling that the best practice is determined by the training environment with the highest fidelity. This approach is hypothesized to provide greater 'transfer value' per simulation trial, by incorporating training techniques and instructional features into the simulator. These techniques allow individuals to acquire these critical skills faster and with greater retention. ARTT also allows an individual trained in 'fast time' to operate at what appears to be a more confident state, when the same task is performed in a real-time environment. Two related experiments are discussed. The findings appear to be consistent with previous findings that show positive effects of task variation during training. Moreover, ARTT has merit in improving or maintaining transfer with sharp reductions in training time. There are indications that the effectiveness of ARTT varies as a function of task content and possibly task difficulty. Other implications for ARTT are discussed along with future research directions

Development of the Steppin\u27 Up for Success Program

School transitions can be challenging for students, especially when students enter junior high school. Junior high school students experience significant developmental growth in the areas of autonomy, relationships, acceptance, cognitive skills, as well as physical differences associated with hormonal changes. When a student transitions into junior high school, evidence indicates possible declines in motivation, school interest, and academic achievement. Personal relationships, self-worth, and coping skills are essential for a successful transition between schools. Adolescents engage in the occupations of education, leisure, social participation, and activities of daily living. Occupational therapy is perfectly positioned as a support service within the school to assist elementary school students transition successfully into junior high school through a universal approach to support positive mental health. This capstone project details the results of a needs assessment conducted with administrators, teachers, parents, and seventh-grade students at the junior high school. Based on the needs assessment, Steppin’ Up forSuccess: A Transition Program for Sixth-Graders was developed and created to assist sixth graders transition successfully from elementary to junior high school

3D tomography of cells in micro-channels

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far been limited to steady specimen, we overcome this restriction and present a method to access the 3D shape of moving objects. The key of our principle is a tilted arrangement of the micro-channel with respect to the focal plane of the microscope. This forces cells to traverse the focal plane in an inclined manner. As a consequence, individual layers of passing cells are recorded which can then be assembled to obtain the volumetric representation. The full 3D information allows for a detailed comparisons with theoretical and numerical predictions unfeasible with e.g.\ 2D imaging. Our technique is exemplified by studying flowing red blood cells in a micro-channel reflecting the conditions prevailing in the microvasculature. We observe two very different types of shapes: `croissants' and `slippers'. Additionally, we perform 3D numerical simulations of our experiment to confirm the observations. Since 3D confocal imaging of cells in flow has not yet been realized, we see high potential in the field of flow cytometry where cell classification thus far mostly relies on 1D scattering and fluorescence signals

Water Pressures and Social Oppression: Social and Political Impacts of Smart Water Meter Technology in Cape Town, South Africa

This paper analyzes the social and political consequences of the implementation of “smart” water meters in Cape Town, South Africa. As policymakers around the world implement new solutions to ever-growing concerns about depleting water resources, technologies and ideas are exported to other parts of the world before experts understand how these concepts will function in different environments. It is imperative to assess the implications of technological solutions to ensure they are implemented sustainably. This paper employs a qualitative approach synthesizing findings from government documents, personal conversations, and traditional and social media to evaluate the societal impacts of smart water meters in Cape Town over the course of the city’s 2017-2018 water crisis. The methodological framework created for this study can be used in future contexts to anticipate how urban areas will respond to smart water technology. This paper concludes with policy recommendations including a historical assessment of water-related grievances, government engagement with business and civil society leaders, and the potential for a cap-and-trade-like system for water rights