The effect of farming system on dairy cow cleanliness in the UK and implications to udder health

The cleanliness of dairy cows was assessed using a 20 point hygiene score system at different times in the year on 14 organic and 14 conventional farms in the UK. Overall, cows were dirtier during winter housing compared to summer grazing. Farming system had no effect on cow cleanliness when cows were at grass, but when housed in the winter, organic cows were more likely to be cleaner. There was a link between cow hygiene scores and milk hygiene, with herds having lower bulk tank somatic cell counts (BTSCC) tending to have cleaner cows. This relationship was strongest for the organic herds. There was no significant link between hygiene score and Bactoscan (BS) count or mastitis incidence

Inter-observer agreement for clinical examinations of foot lesions of sheep

In sheep, the diagnosis of foot lesions is routinely based on physical examination of the hoof. Correct diagnosis is important for the effective treatment, prevention and control of both infectious and non-infectious causes of lameness. Therefore, the aim of this study was to evaluate the level of inter-observer agreement for clinical examination of ovine foot lesions. Eight observers of varying experience, training and occupation performed foot examinations on a total of 1158 sheep from 38 farms across North England and Wales. On each farm, a group of two to four observers independently examined a sample of 24 to 30 sheep to diagnose the presence or absence of specific foot lesions including white line lesions (WL), contagious ovine digital dermatitis (CODD), footrot (FR), inter-digital dermatitis (ID) and toe granuloma (TG). The inter-observer agreement of foot lesion assessments was examined using Fleiss kappa (κ), and Cohen's κ examined the paired agreement between the test standard observer (TSO) and each observer. Scoring differences with the TSO were examined as the percentage of scoring errors and assessed for evidence of systematic scoring bias. With the exception of WL (maximum error rate 33.3%), few scoring differences with the TSO occurred (maximum error rate 3.3%). This suggests that observers can achieve good levels of reliability when diagnosing most of the commonly observed foot conditions associated with lameness in sheep

In vitro susceptibility of contagious ovine digital dermatitis associated Treponema spp. isolates to antimicrobial agents in the UK

Background: Contagious ovine digital dermatitis (CODD) is an important cause of infectious lameness in sheep in the UK and Ireland and has a severe impact on the welfare of affected individuals. The three treponemal phylogroups Treponema medium/Treponema vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes have been associated with clinical CODD lesions and are considered to be a necessary cause of disease. There are scant data on the antimicrobial susceptibility of the treponemes cultured from CODD lesions. Objective: The aim of this study was to determine in vitro the miniumum inhibitory concentration/ minimum bactericidal concentration (MIC/MBC) of antimicrobials used in the sheep industry for isolates of the three CODD associated treponeme phylogroups T. medium/T. vincentii-like, T. phagedenis-like and T. pedis. Animals: Twenty treponeme isolates; from 19 sheep with clinical CODD lesions. Methods: A microdilution method was used to determine in vitro the MIC/MBC of 10 antimicrobial agents for 20 treponeme isolates (five T. medium/T. vincentii-like, 10 T. phagedenis-like and five T. pedis). The antimicrobials tested were penicillin G, amoxicillin, oxytetracycline, tilmicosin, lincomycin, spectinomycin, tylosin, tildipirosin, tulathromycin and gamithromycin. Results: The treponeme isolates tested showed low MICs and MBCs to all 10 antimicrobials tested. They were most susceptible to gamithromycin and tildipirosin (MIC90: 0.0469 mg/L), and were least susceptible to lincomycin, spectinomycin and oxytetracycline (MIC90: 48 mg/L, 24 mg/L and 3 mg/L, respectively). Conclusions: These data are comparable to in vitro antimicrobial susceptibility data for treponemes cultured from bovine digital dermatitis lesions. Dependent on local licensing, penicillin and tilmicosin appear to be the best candidates for future in vivo studies. © 2015 ESVD & ACVD

The broad band spectral properties of galactic X-ray binary pulsars

BeppoSAX observed several galactic binary X-ray pulsars during the Science Verification Phase and in the first year of the regular program. The complex emission spectra of these sources are an ideal target for the BeppoSAX instrumentation, that can measure the emission spectra in an unprecedented broad energy band. Using this capability of BeppoSAX a detailed observational work can be done on the galactic X-ray pulsars. In particular the 0.1-200 keV energy band allows the shape of the continuum emission to be tightly constrained. A better determination of the underlying continuum allows an easier detection of features superimposed onto it, both at low energy (Fe K and L, Ne lines) and at high energies (cyclotron features). We report on the spectral properties of a sample of X-ray pulsars observed with BeppoSAX comparing the obtained results. Some ideas of common properties are also discussed and compared with our present understanding of the emission mechanisms and processes.Comment: 6 pages, 2 figures. Uses espcrc2.sty (included).To appear in Proceedings of "The Active X-ray Sky: Results from BeppoSAX and Rossi-XTE

The broad band spectral properties of binary X-ray pulsars

The X-ray telescopes on board BeppoSAX are an optimal set of instruments to observe bright galactic binary pulsars. These sources emit very hard and quite complex X-ray spectra that can be accurately measured with BeppoSAX between 0.1 and 200 keV. A prototype of this complexity, the source Her X-1, shows at least seven different components in its spectrum. A broad band measure is therefore of paramount importance to have a thorough insight into the physics of the emitting region. Moreover the detection of cyclotron features, when present, allows a direct and highly significant measure of the magnetic field intensity in the emission region. In this paper we briefly report the results obtained with BeppoSAX on this class of sources, with emphasis on the detection and on the measured properties of the cyclotron lines.Comment: 10 Latex pages, 4 figures, uses psfig.sty. Accepted for publication in Advances in Space Research, in Proceedings of 32nd Scientific Assembly of COSPAR - Symposium E1.1: "Broad-Band X-ray Spectroscopy of Cosmic Sources

On the structure of phase transition maps for three or more coexisting phases

This paper is partly based on a lecture delivered by the author at the ERC workshop "Geometric Partial Differential Equations" held in Pisa in September 2012. What is presented here is an expanded version of that lecture.Comment: 23 pages, 6 figure

A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions

Bovine digital dermatitis (BDD) is an infective foot disease commonly reported in dairy cattle where Treponema are considered as the primary causative infectious agents. There still remains little definitive information on the etiology of BDD in beef cattle suggesting further investigations are warranted. Beef BDD lesions (. n=. 34) and healthy beef foot tissues (. n=. 38) were analysed by PCR for three BDD-associated Treponema phylogroups and also for Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was attempted on all BDD lesion samples. One or more BDD-associated Treponema phylogroups were detected in 100 of beef BDD lesions. ". Treponema medium/Treponema vincentii-like", ". Treponema phagedenis-like" and Treponema pedis spirochetes were identified in 27/34 (79), 31/34 (91) and 24/34 (71) of BDD lesions, respectively. No BDD-associated treponeme DNA was amplified from beef healthy foot tissues. D. nodosus and F. necrophorum were present in 24/34 (71) and 15/34 (44) of lesions and 10/38 (26) and 12/38 (32) of healthy foot tissues, respectively. Twenty spirochetes were isolated from beef BDD lesions; 19 were representatives of the three BDD-associated Treponema phylogroups. One spirochete isolate shared less than 97 16S rRNA gene similarity to the three cultivable BDD-associated Treponema phylogroups and therefore may represent a novel taxa of Treponema. Upon comparison, sheep contagious ovine digital dermatitis (CODD), dairy cattle and beef cattle BDD lesions appear to have extremely similar bacteriological data and therefore provides evidence of a shared etiopathogenesis posing concerns for cross-species transmission. © 2015 Elsevier B.V

Bayesian Cluster Finder: Clusters in the CFHTLS Archive Research Survey

The detection of galaxy clusters in present and future surveys enables measuring mass-to-light ratios, clustering properties, galaxy cluster abundances and therefore, constraining cosmological parameters. We present a new technique for detecting galaxy clusters, which is based on the Matched Filter Algorithm from a Bayesian point of view. The method is able to determine the position, redshift and richness of the cluster through the maximization of a filter depending on galaxy luminosity, density and photometric redshift combined with a galaxy cluster prior that accounts for color-magnitude relations and BCG-redshift relation. We tested the algorithm through realistic mock galaxy catalogs, revealing that the detections are 100% complete and 80% pure for clusters up to z $$20 (Abell Richness $\sim$0, M$\sim4\times10^{14} M_{\odot}$). The completeness and purity remains approximately the same if we do not include the prior information, implying that this method is able to detect galaxy cluster with and without a well defined red sequence. We applied the algorithm to the CFHTLS Archive Research Survey (CARS) data, recovering similar detections as previously published using the same or deeper data plus additional clusters which appear to be real.Comment: Accepted for publication in MNRAS; 17 pages, 38 figure

A Spectroscopic and Photometric Study of Short-Timescale Variability in NGC5548

Results of a ground-based optical monitoring campaign on NGC5548 in June 1998 are presented. The broad-band fluxes (U,B,V), and the spectrophotometric optical continuum flux F_lambda(5100 A) monotonically decreased in flux while the broad-band R and I fluxes and the integrated emission-line fluxes of Halpha and Hbeta remained constant to within 5%. On June 22, a short continuum flare was detected in the broad band fluxes. It had an amplitude of about ~18% and it lasted only ~90 min. The broad band fluxes and the optical continuum F_lambda(5100 A) appear to vary simultaneously with the EUV variations. No reliable delay was detected for the broad optical emission lines in response to the EUVE variations. Narrow Hbeta emission features predicted as a signature of an accretion disk were not detected during this campaign. However, there is marginal evidence for a faint feature at lambda = 4962 A with FWHM=~6 A redshifted by Delta v = 1100 km/s with respect to Hbeta_narrow.Comment: 12 pages, 7 figures, accepted for publishing in A&