97 research outputs found

    Sensors and signal processing: An optical edge sensor for transparent film applications

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    Sensing the edge of transparent film webs has always been somewhat difficult. Optical sensors typically see through the low opacity webs while pneumatic, ultrasonic and other sensors have their own sets of unique problems.In transparent film applications, the typical usable optical sensor output can be as low as 10% of that obtained from a normal opaque web. Any error or thermal drift will be amplified through the system electronics along with the desired small control change. If a web were 10% opaque, a 5% thermal drift would become 50% of the usable output signal. Light emitters and sensing elements both have quite dramatic temperature characteristics.By using modulated infrared "IR" techniques along with careful circuit design and stabilization, a sensor and companion amplifier have been developed that is usable with most transparent film web applications. Being low intensity IR in nature, it is useful in some photosensitive film applications. Its temperature drift al less than 1% across the O to 50 C range is the key to transparent film applications.This precision sensor is made possible by utilizing modem design techniques, some of which were borrowed from the aerospace industry. Constructed using Surface Mount Technology (SMT), the carefully selected circuits were fine tuned for stable and predictable operation.The temperature-compensated mini sensor is small in size, low in power consumption, has excellent power supply stability, and temperature stability. The new sensor exhibits good plane change tolerance, ambient light immunity, electrostatic discharge immunity, and an excellent linear proportional control range.The companion X10 amplifier provides the operator with a manual adjustment or control knob to compensate for web opacity. When properly adjusted, this control position relates to the opacity of the web material being used. The primary purpose of the amplifiers is to provide the controller with full-scale or typical sensor levels regardless of web (10% to 100%) opacity. The operator adjustment procedure is quite simple and easily mastered

    Chestnut Living Lab

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    Rebuilding the Fortymile caribou herd: A model of cooperative management planning

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    We examined the public process used to develop the 1996—2001 Fortymile Caribou (Rangifer tarandus) Herd Management Plan adopted by state and federal management boards. The process differed from most government-supported planning processes because it was initiated by residents of Alaska and Yukon, and not by an agency. State, federal, and territorial agencies were asked to participate in and support development of a management plan that would include a broad range of interest groups. We describe the planning effort, issues addressed by the planning team that posed significant challenges during both the planning and implementation phases, and then identify unforeseen costs and benefits derived from the process. Critical decision points in plan development and implementation are discussed

    A shortcut to identifying small molecule signals that regulate behavior and development in Caenorhabditis elegans

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    Small molecule metabolites play important roles in Caenorhabditis elegans biology, but effective approaches for identifying their chemical structures are lacking. Recent studies revealed that a family of glycosides, the ascarosides, differentially regulate C. elegans development and behavior. Low concentrations of ascarosides attract males and thus appear to be part of the C. elegans sex pheromone, whereas higher concentrations induce developmental arrest at the dauer stage, an alternative, nonaging larval stage. The ascarosides act synergistically, which presented challenges for their identification via traditional activity-guided fractionation. As a result the chemical characterization of the dauer and male attracting pheromones remained incomplete. Here, we describe the identification of several additional pheromone components by using a recently developed NMR-spectroscopic approach, differential analysis by 2D NMR spectroscopy (DANS), which simplifies linking small molecule metabolites with their biological function. DANS-based comparison of wild-type C. elegans and a signaling-deficient mutant, daf-22, enabled identification of 3 known and 4 previously undescribed ascarosides, including a compound that features a p-aminobenzoic acid subunit. Biological testing of synthetic samples of these compounds revealed additional evidence for synergy and provided insights into structure–activity relationships. Using a combination of the three most active ascarosides allowed full reconstitution of the male-attracting activity of wild-type pheromone extract. Our results highlight the efficacy of DANS as a method for identifying small-molecule metabolites and placing them within a specific genetic context. This study further supports the hypothesis that ascarosides represent a structurally diverse set of nematode signaling molecules regulating major life history traits

    Nematophagous fungus Arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey

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    To study the molecular basis for predator-prey coevolution, we investigated how Caenorhabditis elegans responds to the predatory fungus Arthrobotrys oligospora. C. elegans and other nematodes were attracted to volatile compounds produced by A. oligospora. Gas-chromatographic mass-spectral analyses of A. oligospora-derived volatile metabolites identified several odors mimicking food cues attractive to nematodes. One compound, methyl 3-methyl-2-butenoate (MMB) additionally triggered strong sex- and stage-specific attraction in several Caenorhabditis species. Furthermore, when MMB is present, it interferes with nematode mating, suggesting that MMB might mimic sex pheromone in Caenorhabditis species. Forward genetic screening suggests that multiple receptors are involved in sensing MMB. Response to fungal odors involves the olfactory neuron AWCs. Single-cell RNA-seq revealed the GPCRs expressed in AWC. We propose that A. oligospora likely evolved the means to use olfactory mimicry to attract its nematode prey through the olfactory neurons in C. elegans and related species

    Nematophagous fungus Arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey

    Get PDF
    To study the molecular basis for predator-prey coevolution, we investigated how Caenorhabditis elegans responds to the predatory fungus Arthrobotrys oligospora. C. elegans and other nematodes were attracted to volatile compounds produced by A. oligospora. Gas-chromatographic mass-spectral analyses of A. oligospora-derived volatile metabolites identified several odors mimicking food cues attractive to nematodes. One compound, methyl 3-methyl-2-butenoate (MMB) additionally triggered strong sex- and stage-specific attraction in several Caenorhabditis species. Furthermore, when MMB is present, it interferes with nematode mating, suggesting that MMB might mimic sex pheromone in Caenorhabditis species. Forward genetic screening suggests that multiple receptors are involved in sensing MMB. Response to fungal odors involves the olfactory neuron AWCs. Single-cell RNA-seq revealed the GPCRs expressed in AWC. We propose that A. oligospora likely evolved the means to use olfactory mimicry to attract its nematode prey through the olfactory neurons in C. elegans and related species
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