Alternativas para a medida do erro na representação do carregamento no método da superposição de vetores de Lanczos

O desenvolvimento de um método de reduçáo de coordenadas, que utiliza uma base constituída por vetores de Lanczos gerados a partir da distribuiçáo espacial do carregamento, suposta fixa, é apresentado. O erro relativo na representasao do carregamento, quando se utiliza a base reduzida, como um critério de interrupçáo na gerasao dos vetores da base, é abordado, mostrando-se que a proposta de Wilson, Yuan e Dickens náo é apropriada. Propóe-se, entiio, duas alternativas para a medida do referido erro relativo, ambas baseadas em normas niio- Euclidianas. Mostra-se que a primeira, por envolver a soluçao de um sistema de equaçóes cuja matriz é a matriz de massa do modelo estrutural, implica em custos computacionais adicionais altos, caso seja utilizada a formulaçáo de matriz de massa consistente. Já a segunda alternativa leva a uma medida para o erro relativo facilmente calculável, pois envolve, apenas, produtos internos entre vetores. Exemplos numéricos sáo apresentados e sugere-se a utilizasao da segunda alternativa.Peer Reviewe

Avaliação do sistema MALDI-TOF MS como metodologia para a identificação de espécies de Rhodotorula spp. : uma revisão da literatura

A maioria dos fungos não-candida não eram considerados patógenos até poucos anos atrás, mas esse cenário está mudando e entre os gêneros que estão emergindo está Rhodotorula: uma levedura que tem atingido principalmente pacientes imunocomprometidos e portadores de cateteres de uso venoso central. Os métodos mais comuns na rotina de análise de micro-organismos são cultura e bioquimismo, seguido de técnicas moleculares, exigindo mais de 48 horas para visualização dos resultados em ambos os casos. Diante da necessidade de resultados mais rápidos a fim de realizar a escolha da terapia correta para o paciente, MALDI-TOF MS vem como uma ferramenta revolucionária, pois através dele é possível obter a identificação correta em menos de 3 horas e em alguns casos em até 20 minutos. No caso de leveduras, há a necessidade de uma etapa pré-analítica muito importante: extração de proteínas; essa técnica pode ser feita por métodos diversos. O objetivo dessa revisão da literatura é abordar aspectos de identificação de espécies de Rhodotorula por MALDI-TOF MS entre os anos 2000 e 2020, traçando uma linha do tempo para que seja possível observar o avanço da técnica, assim como explicar os métodos de extração mais utilizados, os tipos de amostras onde esse micro-organismo foi isolado e as espécies que foram encontradas. Baseado nisso, apesar da necessidade de enriquecimento das espectrotecas, os resultados obtidos foram otimistas, visto que 86% dos estudos foram capazes de identificar Rhodotorula por MALDI e desses, 81,1% conseguiu identificar a nível de espécie, sendo que a predominante foi R. mucilaginosa.Most non-candida fungi were not considered pathogens until a few years ago, but this scenario is changing and among the genus that are emerging is Rhodotorula: a yeast that has mainly affected immunocompromised patients and patients with catheters in use central venous. The most common methods in the routine of microorganism analysis are culture and biochemistry, followed by molecular techniques, requiring more than 48 hours to visualize the results in both cases. In view of the need for faster results in order to choose the right therapy for the patient, MALDI-TOF MS comes as a revolutionary tool, because it is possible to obtain the correct identification in less than 3 hours and in some cases up to 20 minutes. In the case of yeasts, there is a need for a very important pre-analytical step: protein extraction; this technique can be done by different methods. The objective of this literature review is to address aspects of Rhodotorula species identification by MALDI-TOF MS between the years 2000 and 2020, tracing a timeline so that it is possible to observe the advancement of the technique, as well as to explain the most efficient extraction methods used, the types of samples where this microorganism was isolated and the species that were found. Based on this, despite the need to enrich the library of spectral data, the results obtained were optimistic, since 86% of the studies were able to identify Rhodotorula by MALDI and of these, 81.1% managed to identify at a species level, which the predominant was R. mucilaginosa

Testosterone deficiency increases hospital readmission and mortality rates in male patients with heart failure.

BackgroundTestosterone deficiency in patients with heart failure (HF) is associated with decreased exercise capacity and mortality; however, its impact on hospital readmission rate is uncertain. Furthermore, the relationship between testosterone deficiency and sympathetic activation is unknown.ObjectiveWe investigated the role of testosterone level on hospital readmission and mortality rates as well as sympathetic nerve activity in patients with HF.MethodsTotal testosterone (TT) and free testosterone (FT) were measured in 110 hospitalized male patients with a left ventricular ejection fraction < 45% and New York Heart Association classification IV. The patients were placed into low testosterone (LT; n = 66) and normal testosterone (NT; n = 44) groups. Hypogonadism was defined as TT < 300 ng/dL and FT < 131 pmol/L. Muscle sympathetic nerve activity (MSNA) was recorded by microneurography in a subpopulation of 27 patients.ResultsLength of hospital stay was longer in the LT group compared to in the NT group (37 ± 4 vs. 25 ± 4 days; p = 0.008). Similarly, the cumulative hazard of readmission within 1 year was greater in the LT group compared to in the NT group (44% vs. 22%, p = 0.001). In the single-predictor analysis, TT (hazard ratio [HR], 2.77; 95% confidence interval [CI], 1.58-4.85; p = 0.02) predicted hospital readmission within 90 days. In addition, TT (HR, 4.65; 95% CI, 2.67-8.10; p = 0.009) and readmission within 90 days (HR, 3.27; 95% CI, 1.23-8.69; p = 0.02) predicted increased mortality. Neurohumoral activation, as estimated by MSNA, was significantly higher in the LT group compared to in the NT group (65 ± 3 vs. 51 ± 4 bursts/100 heart beats; p < 0.001).ConclusionThese results support the concept that LT is an independent risk factor for hospital readmission within 90 days and increased mortality in patients with HF. Furthermore, increased MSNA was observed in patients with LT

Exercise training prevents the deterioration in the arterial baroreflex control of sympathetic nerve activity in chronic heart failure patients

Arterial baroreflex control of muscle sympathetic nerve activity (ABRMSNA) is impaired in chronic systolic heart failure (CHF). the purpose of the study was to test the hypothesis that exercise training would improve the gain and reduce the time delay of ABRMSNA in CHF patients. Twenty-six CHF patients, New York Heart Association Functional Class II-III, EF <= 40%, peak (V) over dot O-2 <= 20 ml.kg(-1).min(-1) were divided into two groups: untrained (UT, n = 13, 57 +/- 3 years) and exercise trained (ET, n = 13, 49 +/- 3 years). Muscle sympathetic nerve activity (MSNA) was directly recorded by microneurography technique. Arterial pressure was measured on a beat-to-beat basis. Time series of MSNA and systolic arterial pressure were analyzed by autoregressive spectral analysis. the gain and time delay of ABRMSNA was obtained by bivariate autoregressive analysis. Exercise training was performed on a cycle ergometer at moderate intensity, three 60-min sessions per week for 16 wk. Baseline MSNA, gain and time delay of ABRMSNA, and low frequency of MSNA (LFMSNA) to high-frequency ratio (HFMSNA) (LFMSNA/HFMSNA) were similar between groups. ET significantly decreased MSNA. MSNA was unchanged in the UT patients. the gain and time delay of ABRMSNA were unchanged in the ET patients. in contrast, the gain of ABRMSNA was significantly reduced [3.5 +/- 0.7 vs. 1.8 +/- 0.2, arbitrary units (au)/mmHg, P = 0.04] and the time delay of ABRMSNA was significantly increased (4.6 +/- 0.8 vs. 7.9 +/- 1.0 s, P = 0.05) in the UT patients. LFMSNA-to-HFMSNA ratio tended to be lower in the ET patients (P < 0.08). Exercise training prevents the deterioration of ABRMSNA in CHF patients.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundacao ZerbiniCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Heart, Lung, and Blood InstituteUniv São Paulo, Sch Med, Heart Inst InCor, São Paulo, BrazilUniv São Paulo, Sch Phys Educ & Sport, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Cardiol, São Paulo, BrazilUniv Calif Los Angeles, David Geffen Sch Med, Dept Med Cardiol & Physiol, Los Angeles, CA 90095 USAUniversidade Federal de São Paulo, Dept Med, Div Cardiol, São Paulo, BrazilFAPESP: 2010/50048-1FAPESP: 140643/2009-5FAPESP: 2013/07651-7CNPq: 142366/2009-9CNPq: 301867/2010-0CNPq: 308068/2011-4FAPESP: 2013/15651-7National Heart, Lung, and Blood Institute: RO1-HL084525Web of Scienc

Um estudo de tensões e deformações em folhas poliédricas utilizando o método dos elementos finitos

Neste trabalho procura-se desenvolver dois elementos retangulares com o objetivo de se estudar as tensões e deformações que ocorrem em folhas poliédricas, e em estruturas que podem ser aproximadas por meio de uma folha poliédrica. São desenvolvidos dois elementos. Um, segundo a formulação do método dos deslocamentos (modelo compatível) e outro, segundo a formulação híbrida com campo de tensões assumidas. Foram realizados diversos testes, utilizando ambos elementos, e comparando-se os resultados obtidos com resultados experimentais obtidos por diversos experimentadores. A maioria dos testes foi feita com dois tipos de malha para que se tivesse uma idéia da rapidez da convergência dos elementos. São apresentadas numerosas tabelas e gráficos com os resultados.This work is concerned with the development of general space rectangular elements for the study of the stress and deformation behaviour of folded plate-type structures, and also shell structures which can be approximated by an assemblage of plane elements. Two elements are developed. One , following the formulation of the displacement method (compatible model) and the other, following the formulation of the hybrid model with assumed stress fields. Several tests were carried out, using both elements, and comparing the numerical results with experimental results obtained by several other researchers. Most of the tests were done using to types o f meshes. The objective was to have an idea of the convergence rate of the elements. Several tables and graphics are presented with the results

“The things that speak what they want to turn "- Fatinha and the artisanal production in Olhos D’Água (GO)

Submitted by Luciana Ferreira ([email protected]) on 2018-11-07T11:58:19Z No. of bitstreams: 2 Dissertação - Paula Groehs Pfrimer Oliveira Stumpf - 2018.pdf: 3036684 bytes, checksum: 6423899c31805422566b54f5f9a49cb0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2018-11-08T09:53:03Z (GMT) No. of bitstreams: 2 Dissertação - Paula Groehs Pfrimer Oliveira Stumpf - 2018.pdf: 3036684 bytes, checksum: 6423899c31805422566b54f5f9a49cb0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-11-08T09:53:03Z (GMT). No. of bitstreams: 2 Dissertação - Paula Groehs Pfrimer Oliveira Stumpf - 2018.pdf: 3036684 bytes, checksum: 6423899c31805422566b54f5f9a49cb0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-05-22Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESThis dissertation aims through the threads that surround of the handmade objects produced by Fatima Dutra Basto, called Fatinha. She was born and lives in Olhos D'Água, one historically meeting place. Fatinha produces saints and images with corn straw and Banana fiber. There are multiple lines that shrink these objects and Fatinha: Olhos D'Água district, belonging to the municipality of Alexânia-GO, with its history and landscape, Santo Antônio square, the Feira do Troca and local religiosity. We look for these threads, by understanding that object is not isolated in itself, but has stories, from who did it, where it came from, the knowledge and techniques that were developed to the final product. The semi-structured interviews, along with participant observation, helped us to produce the ethnographic elements that will be shared in this dissertation. Thus, among the elements that configure this research, we highlight the place, the craft objects, their technical processes and the narratives of Fatinha, about her history and the objects produced by her. The elements discussed here complement each other and blend together. In this way, we think that in order to understand the craft objects, it is necessary to go through and understand the lines that surround it.Esta dissertação tem como objetivo percorrer os fios que envolvem os objetos artesanais produzidos por Fatinha. Moradora de Olhos D‟Água, historicamente lugar de encontros, Fatinha produz Santos e Imagens com palha de milho e Fibra de bananeira. São múltiplas as linhas que encolvem estes objetos e Fatinha: o distrito de Olhos D‟Água, pertencente ao município de Alexânia-GO, com sua história e paisagem, a praça Santo Antônio, a Feira do troca e a religiosidade local. Buscamos estes fios, por entender que um objeto não é isolado em si, mas possui histórias, de quem o fez, do lugar de onde veio, dos saberes e técnicas que foram desenvolvidos para que se chegasse ao produto final. As entrevista semi-estruturadas, juntamente com a observação participante, nos ajudaram a produzir os elementos etnográficos que serão compartilhados nessa dissertação. Sendo assim, entre os elementos que configuram essa pesquisa, destaca-se, o lugar, os objetos artesanais e seus processos técnicos e as narrativas de Fatinha, acerca de sua história e os objetos produzidos por ela. Os elementos aqui discutidos se complementam e se misturam uns aos outros. Desta maneira, pensamos que para compreender os objetos artesanais, é preciso percorrer e compreender as linhas que o envolvem