Antiglycation activity and HT-29 cellular uptake of aloe-emodin, aloin, and aloe arborescens leaf extracts

Aloe arborescens is a relevant species largely used in traditional medicine of several countries. In particular, the decoction of leaves is prepared for various medicinal purposes including antidiabetic care. The aim of this research was the study of the antiglycation activity of two A. arborescens leaf extracts and isolated compounds: aloin and aloe-emodin. These phytoconstituents were quantitatively assessed in methanolic and hydroalcoholic extracts using high performance liquid chromatography (HPLC) analysis. In addition, the total phenolic and flavonoid contents were detected. In order to study their potential use in diabetic conditions, the antiglycation and antiradical properties of the two extracts and aloin and aloe-emodin were investigated by means of bovine serum albumin (BSA) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) assays; further, their cytotoxicity in HT-29 human colon adenocarcinoma cells was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. Furthermore, the ability of aloin and aloe-emodin to permeate the cellular membranes of HT-29 cells was determined in order to estimate their potential in vivo absorption. This assessment indicated that aloe-emodin can substantially pass through cell membranes (~20%), whereas aloin did not permeate into HT-29 cells. Overall, the data show that both the methanolic and the hydroalcoholic A. arborescens extracts determine significant inhibition of glycation and free-radical persistence, without any cytotoxic activity. The data also show that the antiglycation and the antiradical activities of aloin and aloe-emodin are lower than those of the two extracts. In relation to the permeability study, only aloe-emodin is able to cross HT-29 cellular membranes, showing the attitude to pass through the intestinal layer. Overall, the present data surely support the traditional use of A. arborescens leaf extracts against hyperglycemic conditions, while aloin and aloe-emodin as potential drugs need further study

Observation of sub-natural linewidths for cold atoms in a magneto-optic trap

We have studied the absorption of a weak probe beam through cold rubidium atoms in a magneto-optic trap. The absorption spectrum shows two peaks with the smaller peak having linewidth as small as 28% of the natural linewidth. The modification happens because the laser beams used for trapping also drive the atoms coherently between the ground and excited states. This creates ``dressed'' states whose energies are shifted depending on the strength of the drive. Linewidth narrowing occurs due to quantum coherence between the dressed states. The separation of the states increases with laser intensity and detuning, as expected from this model.Comment: 8 pages, 4 figure

Photometric Solutions for Detached Eclipsing Binaries: selection of ideal distance indicators in the SMC

Detached eclipsing binary stars provide a robust one-step distance determination to nearby galaxies. As a by-product of Galactic microlensing searches, catalogs of thousands of variable stars including eclipsing binaries have been produced by the OGLE, MACHO and EROS collaborations. We present photometric solutions for detached eclipsing binaries in the Small Magellanic Cloud (SMC) discovered by the OGLE collaboration. The solutions were obtained with an automated version of the Wilson-Devinney program. By fitting mock catalogs of eclipsing binaries we find that the normalized stellar radii (particularly their sum) and the surface brightness ratio are accurately described by the fitted parameters and estimated standard errors, despite various systematic uncertainties. In many cases these parameters are well constrained. In addition we find that systems exhibiting complete eclipses can be reliably identified where the fractional standard errors in the radii are small. We present two quantitatively selected sub-samples of eclipsing binaries that will be excellent distance indicators. These can be used both for computation of the distance to the SMC and to probe its structure. One particularly interesting binary has a very well determined solution, exhibits complete eclipses, and is comprised of well detached G-type, class $II$ giants.Comment: 29 pages, 12 figures. To be published in Ap

Optical nonlinear dynamics with cold atoms in a cavity

This paper presents the nonlinear dynamics of laser cooled and trapped cesium atoms placed inside an optical cavity and interacting with a probe light beam slightly detuned from the 6S1/2(F=4) to 6P3/2(F=5) transition. The system exhibits very strong bistability and instabilities. The origin of the latter is found to be a competition between optical pumping and non-linearities due to saturation of the optical transition.Comment: 6 pages, 7 figures, LaTe

The effect of metallicity on the Cepheid distance scale and its implications for the Hubble constant (H0H_0) determination

Recent HST determinations of the expansion's rate of the Universe (the Hubble constant, H_0) assumed that the Cepheid Period-Luminosity relation at V and I are independent of metallicity (Freedman, et al., 1996, Saha et al., 1996, Tanvir et al., 1995). The three groups obtain different vales for H_0. We note that most of this discrepancy stems from the asumption (by both groups) that the Period-Luminosity relation is independent of metallicity. We come to this conclusion as a result of our study of the Period-Luminosity relation of 481 Cepheids with 3 millions two colour measurements in the Large Magellanic Cloud and the Small Magellanic Cloud obtained as a by-product of the EROS microlensing survey. We find that the derived interstellar absorption corrections are particularly sensitive to the metallicity and when our result is applied to recent estimates based on HST Cepheids observations it makes the low-H_0 values higher and the high-H_0 value lower, bringing those discrepant estimates into agrement around $H_0 \approx 70 km/s Mpc^{-1}$.Comment: 4 pages, Latex, with 2 .ps accepted for publication astronomy and astrophysics Letter

Observational Limits on Machos in the Galactic Halo

We present final results from the first phase of the EROS search for gravitational microlensing of stars in the Magellanic Clouds by unseen deflectors (machos: MAssive Compact Halo Objects). The search is sensitive to events with time scales between 15 minutes and 200 days corresponding to deflector masses in the range 1.e-7 to a few solar masses. Two events were observed that are compatible with microlensing by objects of mass of about 0.1 Mo. By comparing the results with the expected number of events for various models of the Galaxy, we conclude that machos in the mass range [1.e-7, 0.02] Mo make up less than 20% (95% C.L.) of the Halo dark matter.Comment: 4 pages, 3 Postscript figures, to be published in Astronomy & Astrophysic

Eclipsing binary stars in the Large Magellanic Cloud : results from the EROS-2, OGLE and VMC surveys

We present a catalogue of 1768 eclipsing binary stars (EBs) detected in the Large Magellanic Cloud (LMC) by the second generation of the EROS survey (hereinafter EROS-2); 493 of them are new discoveries located in outer regions (out of the central bar) of the LMC. These sources were originally included in a list of candidate classical Cepheids (CCs) extracted from the EROS-2 catalogue on the basis of the period (0.89 < 17.82 mag] diagram. After visual inspection of the light curves we reclassified them as eclipsing binaries. They have blue colours (B_EROS - R_EROS < 0.2 mag) hence we classed them as hot eclipsing binaries (HEBs) containing hot massive components: main sequence (MS) stars or blue giants. We present Ks-band light curves for 999 binaries from our sample that have a counterpart in the VISTA near-infrared ESO public survey of the Magellanic Clouds system (VMC). We provide spectral classifications of 13 HEBs with existing spectroscopy. We divided our sample into contact-like binaries and detached/semi-detached systems based on both visual inspection and the parameters of the Fourier decomposition of the light curves and analysed the period-luminosity (PL) relations of the contact-like systems using the R_EROS and Ks magnitudes at maximum light. The contact-like binaries in our sample do not follow PL relations. We analysed the sample of contact binaries from the OGLE III catalogue and confirmed that PL_I and PL_Ks sequences are defined only by eclipsing binaries containing a red giant component.Peer reviewe

The contribution of microlensing surveys to the distance scale

In the early nineties several teams started large scale systematic surveys of the Magellanic Clouds and the Galactic Bulge to search for microlensing effects. As a by product, these groups have created enormous time-series databases of photometric measurements of stars with a temporal sampling duration and accuracy which are unprecedented. They provide the opportunity to test the accuracy of primary distance indicators, such as Cepheids, RRLyrae stars, the detached eclipsing binaries, or the luminosity of the red clump. We will review the contribution of the microlensing surveys to the understanding of the physics of the primary distance indicators, recent differential studies and direct distance determinations to the Magellanic Clouds and the Galactic Bulge.Comment: Invited review article to appear in: `Post-Hipparcos Cosmic Candles', A. Heck & F. Caputo (Eds), Kluwer Academic Publ., Dordrecht, in press. 21 pages; uses Kluwer's crckapb.sty LaTeX style file, enclose