Quantitative full-colour transmitted light microscopy and dyes for concentration mapping and measurement of diffusion coefficients in microfluidic architectures

International audienceA simple and versatile methodology has been developed for the simultaneous measurement of multiple concentration profiles of colourants in transparent microfluidic systems, using a conventional transmitted light microscope, a digital colour (RGB) camera and numerical image processing combined with multicomponent analysis. Rigorous application of the Beer-Lambert law would require monochromatic probe conditions, but in spite of the broad spectral bandwidths of the three colour channels of the camera, a linear relation between the measured optical density and dye concentration is established under certain conditions. An optimised collection of dye solutions for the quantitative optical microscopic characterisation of microfluidic devices is proposed. Using the methodology for optical concentration measurement we then implement and validate a simplified and robust method for the microfluidic measurement of diffusion coefficients using an H-filter architecture. It consists of measuring the ratio of the concentrations of the two output channels of the H-filter. It enables facile determination of the diffusion coefficient, even for non-fluorescent molecules and nanoparticles, and is compatible with non-optical detection of the analyte

Predictive toxicology using systemic biology and liver microfluidic "on chip" approaches: Application to acetaminophen injury

International audienceWe have analyzed transcriptomic, proteomic and metabolomic profiles of hepatoma cells cultivated inside a microfluidic biochip with or without acetaminophen (APAP). Without APAP, the results show an adaptive cellular response to the microfluidic environment, leading to the induction of anti-oxidative stress and cytoprotective pathways. In presence of APAP, calcium homeostasis perturbation, lipid peroxidation and cell death are observed. These effects can be attributed to APAP metabolism into its highly reactive metabolite. N-acetyl-p-benzoquinone imine (NAPQI). That toxicity pathway was confirmed by the detection of GSH-APAP, the large production of 2-hydroxybutyrate and 3-hydroxybutyrate, and methionine, cystine, and histidine consumption in the treated biochips. Those metabolites have been reported as specific biomarkers of hepatotoxicity and glutathione depletion in the literature. In addition, the integration of the metabolomic, transcriptomic and proteomic collected profiles allowed a more complete reconstruction of the APAP injury pathways. To our knowledge, this work is the first example of a global integration of microfluidic biochip data in toxicity assessment. Our results demonstrate the potential of that new approach to predictive toxicology

Contribution of the land sector to a 1.5 °C world

Acknowledgements The analysis in this study was guided by the valuable feedback and recommendations of expert consultations and interviews, and we extend our gratitude to all those individuals who contributed to our research and analysis: Jeff Atkins (Virginia Commonwealth University), Jonah Busch (Earth Innovation Institute), Peter Ellis (The Nature Conservancy), Jason Funk (Center for Carbon Removal), Trisha Gopalakrishna (The Nature Conservancy), Alan Kroeger (Climate Focus), Bernice Lee (Chatham House), Donna Lee (Climate and Land Use Alliance), Simon Lewis (University College London), Guy Lomax (The Nature Conservancy), Dann Mitchell (University of Bristol), Raoni Rajão (University of Minas Gerais), Joeri Rogelj (IIASA), Carl-Friedrich Schleussner (Climate Analytics), Paul West (University of Minnesota), Graham Wynne (Prince of Wales International Sustainability Unit), Ana Yang (Children’s Investment Fund Foundation) and Dan Zarin (Climate and Land Use Alliance). A special thank you to Esther Chak and Mary-Jo Valentino (Imaginary Office) for designing the figures in this study. This work was generously supported by the Children’s Investment Fund Foundation and the authors’ institutions and funding sources.Peer reviewedPostprin

論説 : Micromachines for Cell Manipulation

工学とバイ

An easy-to-build and re-usable microfluidic system for live-cell imaging

Abstract Background Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central to cell biology. However, when coupled to live-cell imaging, such strategies are difficult to implement with precision and high time resolution, and the simultaneous alteration of multiple parameters is a major challenge. Recently, microfluidics has provided powerful solutions for such analyses, bringing an unprecedented level of control over the conditions and the medium in which cells under microscopic observation are grown. However, such technologies have remained under-exploited, largely as a result of the complexity associated with microfabrication procedures. Results In this study, we have developed simple but powerful microfluidic devices dedicated to live-cell imaging. These microsystems take advantage of a robust elastomer that is readily available to researchers and that presents excellent bonding properties, in particular to microscopy-grade glass coverslips. Importantly, the chips are easy-to-build without sophisticated equipment, and they are compatible with the integration of complex, customized fluidic networks as well as with the multiplexing of independent assays on a single device. We show that the chips are re-usable, a significant advantage for the popularization of microfluidics in cell biology. Moreover, we demonstrate that they allow for the dynamic, accurate and simultaneous control of multiple parameters of the cellular environment. Conclusions While they do not possess all the features of the microdevices that are built using complex and costly procedures, the simplicity and versatility of the chips that we have developed make them an attractive alternative for a range of applications. The emergence of such devices, which can be fabricated and used by any laboratory, will provide the possibility for a larger number of research teams to take full advantage of these new methods for investigating cell biology

Optical study of halide modified sulfide glasses containing neodymium ions

Optical properties of different halide modified germanium gallium sulfide glasses doped with Nd3+ ions are presented. Glasses studied were of the compositions: 50GeS(2)-25Ga(2)S(3)-25CsX, doped with 0.5 at.% Nd3+ where X is Cl, Br or I. Absorption spectra were measured and oscillator strengths calculated. Excited-state emission lifetimes were calculated by the Judd-Ofelt theory and compared with the measured lifetimes. Results were compared with a pure sulfide glass of the composition: Ge25Ga5S70. The halide modified glasses have been found to present a unexpected reversible water sensitivity.status: publishe

Double-Height Accurate Micro-Molding Method for Three-Dimensional PDMS Structures

International audienceA fabrication method for making accurate double-height micromolds is presented. Fine features of the micromold are transferred to a three-dimensional poly-dimethylsiloxane (PDMS) microfluidic membrane. The accuracy of features is within 1.55% and the maximum aspect ratio is 7. In this work, the first layer of the micromolds is made directly on silicon wafers by inductively coupled plasma reactive ion etching (ICP-RIE). The second layer is added by photolithography of SU-8 negative photoresist on top of the first layer. This method allows the fabrication of micromolds having wall dimensions as small as 3 mm that was not previously achievable. Such dimensions are required in biological microfluidic systems to reduce the amount of chemicals or to confine cells to a desired position

Micro-chambres d'analyses chimique et biologique : microcapteurs de mesure de pH et microfluidique associés

National audienc

Micro-chambres d'analyses chimique et biologique : microcapteurs de mesure de pH et microfluidique associés

National audienc