Benefit Cost Analysis of RD&E in Action

Since 2004, the Queensland Department of Primary Industries and Fisheries has embarked on a rigorous evaluation of its R&D Programs, with Benefit Cost Analysis being a cornerstone of this work. The analyses conducted to date have proved to be powerful tools in internal resource re-allocation. However, the process used has been time consuming, partly because there needs to be adequate time allowed for consultation both with scientists and with senior staff. Experience has highlighted the need for strong organisational support to the analysts carrying out the work.Benefit Cost Analysis, management, research and development, Research and Development/Tech Change/Emerging Technologies,

Urban principal creative leadership and policy compliance in the era of accountability

For decades, urban school reform has been a persistent issue. Research suggests that urban school reforms that connect equitably to broader community improvement efforts are more sustainable and that principals play a pivotal role in leading such efforts. Although the role of the school principal is a front-line leader charged with the execution of policy and legislation, the experience of principal leadership is an area of limited research particularly how the creative leadership of the school principal connects with school transformational improvement efforts. The purpose of this research was to explore and describe the experiences of urban principals, particularly their roles, responsibilities and leadership styles within an era of accountability of student performance outcomes as measured in state test scores. Central to the study was the principal narrations of their experiences as they navigated between policy compliance and creative leadership through the specific context of the Cleveland Plan implementation. In this study, creative leadership is defined as a multidimensional and transformational in its integration of distributed, authentic, and adaptive in its response to complex urban environments. It involves a view towards change that steps outside of the existing practices through collaborative, distributed, and authentic leadership to strategically move through a problem-solving framework (Puccio, Mance, & Murdock, 2011). vii Using a case study design, the research focused on the principal leadership skill set as narrated by principals within a context of a specific period of school reform beginning when the Cleveland Plan was legislated as H.B. 525 in 2012 through 2019 and the issuance of the last full year state report card. Data collection involved semistructured interviews with principals of the Cleveland Metropolitan School District. The research sought to both expand the study of school administration and leadership in new directions and to contribute to the base of research using the actual experiences of urban principals

Brownlee, Margaret

Margaret Brownlee is a multi-racial/Afro-Latina Lipstick Lesbian who was born and spent most of her life residing in Maine. Margaret was 16 when she came out and is now married to a woman and has a daughter. Margaret attended multiple universities–including Wells College, Lesley University, and the University of New England–as a first generation college student in her family with the goal of becoming a dancer. Margaret is currently a Burlesque dancer and instructor and is also employed with the Maine Department of Education. She has been involved in political activism and a number of organizations based in Maine–including Portland Outright, EqualityMaine, EmergeMaine, South Portland Human Rights Commission, Maine Black Community Development, and Indigo Arts.https://digitalcommons.usm.maine.edu/querying_ohproject/1084/thumbnail.jp

Phosphorylation of Spinophilin Modulates Its Interaction with Actin Filaments

Spinophilin is a protein phosphatase 1 (PP1)- and actin-binding protein that modulates excitatory synaptic transmission and dendritic spine morphology. We report that spinophilin is phosphorylated in vitro by protein kinase A (PKA). Phosphorylation of spinophilin was stimulated by treatment of neostriatal neurons with a dopamine D1 receptor agonist or with forskolin, consistent with spinophilin being a substrate for PKA in intact cells. Using tryptic phosphopeptide mapping, site-directed mutagenesis, and microsequencing analysis, we identified two major sites of phosphorylation, Ser-94 and Ser-177, that are located within the actin-binding domain of spinophilin. Phosphorylation of spinophilin by PKA modulated the association between spinophilin and the actin cytoskeleton. Following subcellular fractionation, unphosphorylated spinophilin was enriched in the postsynaptic density, whereas a pool of phosphorylated spinophilin was found in the cytosol. F-actin co-sedimentation and overlay analysis revealed that phosphorylation of spinophilin reduced the stoichiometry of the spinophilin-actin interaction. In contrast, the ability of spinophilin to bind to PP1 remained unchanged. Taken together, our studies suggest that phosphorylation of spinophilin by PKA modulates the anchoring of the spinophilin-PP1 complex within dendritic spines, thereby likely contributing to the efficacy and plasticity of synaptic transmission

Turf wars: experimental tests for alternative stable states in a two-phase coastal ecosystem

Alternative stable states have long been thought to exist in natural communities, but direct evidence for their presence and for the environmental switches that cause them has been scarce. Using a combination of greenhouse and field experiments, we investigated the environmental drivers associated with two distinctive herbaceous communities in coastal ecosystems in New Zealand. In a mosaic unrelated to micro-topography, a community dominated largely by native turf species (notably Leptinella dioica, Samolus repens, and Selliera radicans) alternates with vegetation comprising exotic (i.e., nonnative) pasture species (notably Agrostis stolonifera, Holcus lanatus, Lolium perenne, and Trifolium repens). The species of these two communities differ in functional characters related to leaf longevity and growth rate, and occupy soils of differing nitrogen levels. Both spatial and environmental factors influenced the species composition locally. Reciprocal transplants of soil, with and without associated vegetation, showed that a native turf community developed when sward or soil from either community was bounded by turf, and a pasture community developed when sward or soil from either community was surrounded by pasture. In artificial mixed communities in the greenhouse, turf was able to invade the pasture community where the vegetation was clipped to simulate grazing, and also where Trifolium was removed and/or salt spray was applied. The pasture community invaded the turf where Trifolium was present or nitrogen was added. These results were supported by trends in experimentally manipulated field plots, where the amount of turf cover increased when nitrogen was kept low and when salt spray was applied, whereas pasture cover increased in the absence of salt spray. Thus, persistence of the native turf community is dependent on grazing, both directly and via its effect on keeping nitrogen levels low by excluding the exotic, nitrogen-fixing Trifolium, and by exposing the vegetation to salt spray. If any of these factors change, there could be a state change to pasture dominance that might be resistant to reversion to turf. Managing such coastal herbaceous communities therefore requires an understanding of the environmental and species characteristics that maintain alternative states

Inference of the Arabidopsis Lateral Root Gene Regulatory Network Suggests a Bifurcation Mechanism That Defines Primordia Flanking and Central Zones

A large number of genes involved in lateral root (LR) organogenesis have been identified over the last decade using forward and reverse genetic approaches in Arabidopsis thaliana. Nevertheless, how these genes interact to form a LR regulatory network largely remains to be elucidated. In this study, we developed a time-delay correlation algorithm (TDCor) to infer the gene regulatory network (GRN) controlling LR primordium initiation and patterning in Arabidopsis from a time-series transcriptomic data set. The predicted network topology links the very early-activated genes involved in LR initiation to later expressed cell identity markers through a multistep genetic cascade exhibiting both positive and negative feedback loops. The predictions were tested for the key transcriptional regulator AUXIN RESPONSE FACTOR7 node, and over 70% of its targets were validated experimentally. Intriguingly, the predicted GRN revealed a mutual inhibition between the ARF7 and ARF5 modules that would control an early bifurcation between two cell fates. Analyses of the expression pattern of ARF7 and ARF5 targets suggest that this patterning mechanism controls flanking and central zone specification in Arabidopsis LR primordia

Structural parameters for globular clusters in NGC 5128. III. ACS surface-brightness profiles and model fits

We present internal surface-brightness profiles, based on HST/ACS imaging in the F606W bandpass, for 131 globular cluster (GC) candidates with luminosities 10^4 - 3 x 10^6 solar, in the giant elliptical galaxy NGC 5128. Several structural models are fit to the profile of each cluster and combined with mass-to-light ratios from population-synthesis models, to derive a catalogue of fundamental structural and dynamical parameters parallel in form to the catalogues recently produced by McLaughlin & van der Marel and Barmby et al. for GCs and massive young star clusters in Local Group galaxies. As part of this, we provide corrected and extended parameter estimates for another 18 clusters in NGC 5128, which we observed previously. We show that, like GCs in the Milky Way and some of its satellites, the majority of globulars in NGC 5128 are well fit by isotropic Wilson models, which have intrinsically more distended envelope structures than the standard King lowered isothermal spheres. We use our models to predict internal velocity dispersions for every cluster in our sample. These predictions agree well in general with the observed dispersions in a small number of clusters for which spectroscopic data are available. In a subsequent paper, we use these results to investigate scaling relations for GCs in NGC 5128.Comment: MNRAS, in press. 28 pages. Full data tables available at http://www.astro.keele.ac.uk/~dem/clusters.htm

Cardiac Gene Transfer of Short Hairpin RNA Directed Against Phospholamban Effectively Knocks Down Gene Expression but Causes Cellular Toxicity in Canines

Derangements in calcium cycling have been described in failing hearts, and preclinical studies have suggested that therapies aimed at correcting this defect can lead to improvements in cardiac function and survival. One strategy to improve calcium cycling would be to inhibit phospholamban (PLB), the negative regulator of SERCA2a that is upregulated in failing hearts. The goal of this study was to evaluate the safety and efficacy of using adeno-associated virus (AAV)-mediated cardiac gene transfer of short hairpin RNA (shRNA) to knock down expression of PLB. Six dogs were treated with self-complementary AAV serotype 6 (scAAV6) expressing shRNA against PLB. Three control dogs were treated with empty AAV6 capsid, and two control dogs were treated with scAAV6 expressing dominant negative PLB. Vector was delivered via a percutaneously inserted cardiac injection catheter. PLB mRNA and protein expression were analyzed in three of six shRNA dogs between days 16 and 26. The other three shRNA dogs and five control dogs were monitored long-term to assess cardiac safety. PLB mRNA was reduced 16-fold, and PLB protein was reduced 5-fold, with treatment. Serum troponin elevation and depressed cardiac function were observed in the shRNA group only at 4 weeks. An enzyme-linked immunospot assay failed to detect any T cells reactive to AAV6 capsid in peripheral blood mononuclear cells, heart, or spleen. Microarray analysis revealed alterations in cardiac expression of several microRNAs with shRNA treatment. AAV6-mediated cardiac gene transfer of shRNA effectively knocks down PLB expression but is associated with severe cardiac toxicity. Toxicity may result from dysregulation of endogenous microRNA pathways