49 research outputs found
Intensive care services in Hungary 2000 to 2010: an analysis of bed numbers, occupancy rates, case mix and economics
Localization of AQP5 during development of the mouse submandibular salivary gland
Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution of AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues to its unknown role during development. In the present work, SMGs from embryonic day (E) 14.5–18.5 and postnatal days (P) 0, 2, 5, 25, and 60 were immunostained for AQP5 and analyzed using light microscopy. Additional confocal and transmission electron microscopy were performed on P60 glands. Our results show that AQP5 expression first occurs in a scattered pattern in the late canalicular stage and becomes more prominent and organized in the terminal tubuli/pro-acinar cells towards birth. Additional apical membrane staining in the entire intralobular duct is found just prior to birth. During postnatal development, AQP5 is expressed in both the luminal and lateral membrane of pro-acinar/acinar cells. AQP5 is also detected in the basal membrane of acinar cells at P25 and P60. In the intercalated ducts at P60, the male glands show apical staining in the entire segment, while only the proximal region is positive in the female glands. These results demonstrate an evolving distribution of AQP5 during pre- and postnatal development in the mouse SMGs
Human aquaporins: regulators of transcellular water flow
Background: Emerging evidence supports the view that (AQP) aquaporin water channels are regulators of transcellular
water flow. Consistentwith their expression in most tissues, AQPs are associatedwith diverse physiological
and pathophysiological processes.
Scope of review: AQP knockout studies suggest that the regulatory role of AQPs, rather than their action as passive
channels, is their critical function. Transport through all AQPs occurs by a common passive mechanism, but their
regulation and cellular distribution varies significantly depending on cell and tissue type; the role of AQPs in cell
volumeregulation (CVR) is particularly notable. This reviewexamines the regulatory role of AQPs in transcellular
water flow, especially in CVR.We focus on key systems of the human body, encompassing processes as diverse as
urine concentration in the kidney to clearance of brain oedema.
Major conclusions: AQPs are crucial for the regulation of water homeostasis, providing selective pores for the
rapidmovement ofwater across diverse cellmembranes and playing regulatory roles in CVR. Gatingmechanisms
have been proposed for human AQPs, but have only been reported for plant andmicrobial AQPs. Consequently, it
is likely that the distribution and abundance of AQPs in a particular membrane is the determinant of membrane
water permeability and a regulator of transcellular water flow.
General significance: Elucidating the mechanisms that regulate transcellular water flow will improve our understanding
of the human body in health and disease. The central role of specific AQPs in regulating water homeostasis
will provide routes to a range of novel therapies. This article is part of a Special Issue entitled Aquaporins