The Public Health Impact of Coronavirus Disease on Human Trafficking

The global pandemic of severe acute respiratory syndrome coronavirus 2 exacerbates major risk factors for global human trafficking. Social isolation of families and severe economic distress amplify the risk of interpersonal violence, unemployment and homelessness, as well as increased internet use by under-supervised children. Aggravating the situation are overwhelmed health systems, severe limitations in activities of social service organizations, and decreased contact of healthcare professionals with children. Healthcare professionals have a duty to be alert to possible indicators of trafficking, and aware of available victim resources which can be offered to at-risk patients. Healthcare facilities should take steps to increase public awareness of trafficking and community resources

A Screening tool for Identification of Victims of Commercial Sexual Exploitation of Children

Background: Commercial sexual exploitation of children (CSEC) and sex trafficking have only recently been recognized as problems by healthcare providers. Both UNICEF (2014) and the Institute of Medicine (2013) have stressed the need for systematic research to assist healthcare providers in the identification of victims. The aim of this poster is to describe initial findings relating to the development of a screening tool to identify CSEC victims. Methods: Twenty-seven sites nationwide (e.g., emergency departments and specialized clinics) participated in a study to validate a screening tool for identifying CSEC victims in an outpatient setting. The study was conducted under approval from the Institutional Review Board at Children’s Healthcare of Atlanta. Inclusion criteria for the study generally involved being an English-speaking adolescent aged 11-18 years. Results: Study enrollment is ongoing. Preliminary data for 210 youth were analyzed for this abstract. The sample was diverse with respect to age (M=14.59 years, SD=1.490 years) and ethnicity (56.4% Caucasian, 31.8% African American, 3.9% mixed race, and 7.8% Hispanic); the sample was predominantly female (92.8%). Of the 210 youth in the sample, 115(54.8%) have had sex. Of these 115 youth, 13(11.3%) have traded sex for money, drugs, or housing; 7(58.3%) of 12(10.4%) complied when asked by someone to have sex with another person; 8(61.5%) of 13(11.3%) performed sexual acts in public when propositioned; and 19(45.2%) of 42(36.5%) shared provocative photos when prompted. Medical providers flagged 14 youth (6.7% of total 210) as potential CSEC victims. Conclusions: The screening tool shows promise for effective identification of CSEC victims. This poster will present additional data and further quantitative analyses exploring the influences of sexual behavior, drug and alcohol use, and other factors on the risk of becoming a CSEC victim. Implications for researchers and clinicians will also be discussed

Exploring the Recovery Lakes region and interior Dronning Maud Land, East Antarctica, with airborne gravity, magnetic and radar measurements

Long-range airborne geophysical measurements were carried out in the ICEGRAV campaigns, covering hitherto unexplored parts of interior East Antarctica and part of the Antarctic Peninsula. The airborne surveys provided a regional coverage of gravity, magnetic and ice-penetrating radar measurements for major Dronning Maud Land ice stream systems, from the grounding lines up to the Recovery Lakes drainage basin, and filled in major data voids in Antarctic data compilations, such as AntGP for gravity data, ADMAP for magnetic data and BEDMAP2 for ice thickness data and the sub-ice topography. We present the first maps of gravity, magnetic and ice thickness data and bedrock topography for the region and show examples of bedrock topography and basal reflectivity patterns. The 2013 Recovery Lakes campaign was carried out with a British Antarctic Survey Twin Otter aircraft operating from the Halley and Belgrano II stations, as well as a remote field camp located at the Recovery subglacial Lake B site. Gravity measurements were the primary driver for the survey, with two airborne gravimeters (Lacoste and Romberg and Chekan-AM) providing measurements at an accuracy level of around 2 mGal r.m.s., supplementing GOCE (Gravity Field and Steady-State Ocean Circulation Explorer) satellite data and confirming an excellent sub-milligal agreement between satellite and airborne data at longer wavelengths

New Magnetic Anomaly Map of the Antarctic

The second generation Antarctic magnetic anomaly compilation for the region south of 60 degrees S includes some 3.5 million line-km of aeromagnetic and marine magnetic data that more than doubles the initial map's near-surface database. For the new compilation, the magnetic data sets were corrected for the International Geomagnetic Reference Field, diurnal effects, and high-frequency errors and leveled, gridded, and stitched together. The new magnetic data further constrain the crustal architecture and geological evolution of the Antarctic Peninsula and the West Antarctic Rift System in West Antarctica, as well as Dronning Maud Land, the Gamburtsev Subglacial Mountains, the Prince Charles Mountains, Princess Elizabeth Land, and Wilkes Land in East Antarctica and the circumjacent oceanic margins. Overall, the magnetic anomaly compilation helps unify disparate regional geologic and geophysical studies by providing new constraints on major tectonic and magmatic processes that affected the Antarctic from Precambrian to Cenozoic times.Korea Polar Research Institute (KOPRI) programs, PM15040 and PE17050Germany's AWI/Helmholtz Center for Polar and Marine ResearchFederal Institute for Geosciences and Natural ResourcesBritish Antarctic Survey/Natural Environmental Research CouncilItalian Antarctic Research ProgrammeRussian Ministry of Natural ResourcesU.S. National Science Foundation and National Space and Aeronautics AdministrationAustralian Antarctic Division and Antarctic Climate & Ecosystem Cooperative Research CentreFrench Polar InstituteGlobal geomagnetic observatories network (INTERMAGNET

Evolution of the Sequence Composition of Flaviviruses

The adaption of pathogens to their host(s) is a major factor in the emergence of infectious disease and the persistent survival of many of the infectious diseases within the population. Since many of the smaller viral pathogens are entirely dependent upon host machinery, it has been postulated that they are under selection for a composition similar to that of their host. Analyses of sequence composition have been conducted for numerous small viral species including the Flavivirus genus. Examination of the species within this particular genus that infect vertebrate hosts revealed that sequence composition proclivities do not correspond with vector transmission as the evolutionary history of this species suggests. Recent sequencing efforts have generated complete genomes for many viral species including members of the Flavivirus genus. A thorough comparison of the sequence composition was conducted for all of the available Flaviviruses for which the complete genome is publicly available. This effort expands the work of previous studies to include new vector-borne species as well as members of the insect-specific group which previously have not been explored. Metrics, including mono-, di-, and trinucleotide abundances as well as NC values and codon usage preferences, were explored both for the entire polyprotein sequence as well as for each individual coding region. Preferences for compositions correspond to host-range rather than evolutionary history; species which infect vertebrate hosts exhibited particular preferences similar to each other as well as in correspondence with their host’s preferences. Flaviviruses which do not infect vertebrate hosts, however, did not show these proclivities, with the exception of the Kamiti River virus suggesting its recent (either past or present) infectivity of an unknown vertebrate host

Clinical care of childhood sexual abuse: a systematic review and critical appraisal of guidelines from European countries

Background: The clinical management of Child sexual abuse (CSA) demands specialised skills from healthcare professionals due to its sensitivity, legal implications, and serious physical health and mental health effects. Standardised, comprehensive clinical practice guidelines (CPGs) may be pivotal. In this systematic review, we examined existing CSA national CPGs (NCPGs) from European countries to assess their quality and reporting. Methods: We systematically searched six international databases and multiple grey literature sources, reporting by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards. Eligible guidelines were CSA guidance from national health agencies or societies in 34 COST Action 19106 Network Countries (CANC), published between January 2012 and November 2022. Two independent researchers searched, screened, reviewed, and extracted data. NCPGs were compared for completeness with reference WHO 2017 and 2019 guidelines. We used the Appraisal of Guidelines for Research and Evaluation (AGREE II) to appraise quality and reporting. PROSPERO: CRD42022320747. Findings: Of 2919 records identified by database searches, none met inclusion criteria. Of 4714 records identified by other methods, 24 NCPGs from 17 (50%) of CANC countries were included. In 17 (50%) of eligible countries, no NCPGs were found. Content varied significantly within and between countries. NCPGs lacked many components in state-of-the art clinical practice compared to WHO reference standards, particularly in safety and risk assessment, interactions with caregivers, and mental health interventions. Appraisal by AGREE II revealed shortcomings in NCPG development, regarding scientific rigour, stakeholder involvement, implementation and evaluation. Interpretation: A notable number of European countries lack an NCPG; existing NCPGs often fall short. The healthcare response to CSA in Europe requires a coordinated approach to develop and implement high-quality CPGs. We advocate for a multidisciplinary team to develop a pan-European CSA guideline to ensure quality care for survivors

Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies

Heart rate is under the precise control of the autonomic nervous system. However, the wiring of peripheral neural circuits that regulate heart rate is poorly understood. Here, we develop a clearing-imaging-analysis pipeline to visualize innervation of intact hearts in 3D and employed a multi-technique approach to map parasympathetic and sympathetic neural circuits that control heart rate in mice. We identify cholinergic neurons and noradrenergic neurons in an intrinsic cardiac ganglion and the stellate ganglia, respectively, that project to the sinoatrial node. We also report that the heart rate response to optogenetic versus electrical stimulation of the vagus nerve displays different temporal characteristics and that vagal afferents enhance parasympathetic and reduce sympathetic tone to the heart via central mechanisms. Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs

Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies

Heart rate is under the precise control of the autonomic nervous system. However, the wiring of peripheral neural circuits that regulate heart rate is poorly understood. Here, we develop a clearing-imaging-analysis pipeline to visualize innervation of intact hearts in 3D and employed a multi-technique approach to map parasympathetic and sympathetic neural circuits that control heart rate in mice. We identify cholinergic neurons and noradrenergic neurons in an intrinsic cardiac ganglion and the stellate ganglia, respectively, that project to the sinoatrial node. We also report that the heart rate response to optogenetic versus electrical stimulation of the vagus nerve displays different temporal characteristics and that vagal afferents enhance parasympathetic and reduce sympathetic tone to the heart via central mechanisms. Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs

Gene Expression Divergence is Coupled to Evolution of DNA Structure in Coding Regions

Sequence changes in coding region and regulatory region of the gene itself (cis) determine most of gene expression divergence between closely related species. But gene expression divergence between yeast species is not correlated with evolution of primary nucleotide sequence. This indicates that other factors in cis direct gene expression divergence. Here, we studied the contribution of DNA three-dimensional structural evolution as cis to gene expression divergence. We found that the evolution of DNA structure in coding regions and gene expression divergence are correlated in yeast. Similar result was also observed between Drosophila species. DNA structure is associated with the binding of chromatin remodelers and histone modifiers to DNA sequences in coding regions, which influence RNA polymerase II occupancy that controls gene expression level. We also found that genes with similar DNA structures are involved in the same biological process and function. These results reveal the previously unappreciated roles of DNA structure as cis-effects in gene expression

Quantitative Proteomic and Interaction Network Analysis of Cisplatin Resistance in HeLa Cells

Cisplatin along with other platinum based drugs are some of the most widely used chemotherapeutic agents. However drug resistance is a major problem for the successful chemotherapeutic treatment of cancer. Current evidence suggests that drug resistance is a multifactorial problem due to changes in the expression levels and activity of a wide number of proteins. A majority of the studies to date have quantified mRNA levels between drug resistant and drug sensitive cell lines. Unfortunately mRNA levels do not always correlate with protein expression levels due to post-transcriptional changes in protein abundance. Therefore global quantitative proteomics screens are needed to identify the protein targets that are differentially expressed in drug resistant cell lines. Here we employ a quantitative proteomics technique using stable isotope labeling with amino acids in cell culture (SILAC) coupled with mass spectrometry to quantify changes in protein levels between cisplatin resistant (HeLa/CDDP) and sensitive HeLa cells in an unbiased fashion. A total of 856 proteins were identified and quantified, with 374 displaying significantly altered expression levels between the cell lines. Expression level data was then integrated with a network of protein-protein interactions, and biological pathways to obtain a systems level view of proteome changes which occur with cisplatin resistance. Several of these proteins have been previously implicated in resistance towards platinum-based and other drugs, while many represent new potential markers or therapeutic targets