Integrating Shotgun Metagenomics, 16s Rrna Gene Metabarcoding and Culture Approaches: A Better Outlook for Functional Profiling of a Pah-Contaminated Soil

Understanding bacterial diversity and function is critical for designing bioremediation strategies. This research aimed to assess chronically hydrocarbon contaminated soil bacterial diversity and their aromatic compound degradation (ACD) potential by integrating shotgun metagenomic, 16S rRNA gene metabarcoding and culture approaches. While soil metabarcoding showed dominance of Proteobacteria, metagenomics indicated that 99,5% of the sequences were taxonomically assigned to Streptomycetales order and that almost all genes related to ACD were assigned to the latter. To inspect other phyla contribution to ACD, a functional prediction was delved, and two culture approaches were used. PICRUSt2 revealed that ACD pathways were mostly found in Alphaproteobacteria, Actinobacteria and Gammaproteobacteria classes. An enrichment culture (r-EFP) was obtained with pyrene as sole carbon and energy source and a bacterial strain (S19P6), identified as a member of Mycolicibacterium genus, was isolated. Both cultures demonstrated the ability to degrade more than 90% of the supplemented pyrene after 21 days of incubation. 16S rRNA gene metabarcoding and shotgun metagenomics approaches in r-EFP indicated predominance of Proteobacteria Phylum and the presence of genes responsible for the degradation of ACD mostly assigned to the predominant phyla. Complementing different methodologies enable the recognition of the metabolic potential of soil Proteobacteria related to ACD.Fil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Granada, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Irazoqui, José Matías. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Cuadros Orellana, Sara. Universidad Catolica de Maule; ChileFil: Quevedo, Claudio. Universidad Catolica de Maule; ChileFil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin

ASPECTOS QUE INTERACTUAN EN EL PROCESO ADAPTATIVO ENTRE NIÑOS EN SITUACIÓN DE DESPLAZAMIENTO Y SUS NUEVOS ENTORNOS

Este artículo describe la percepción organizada por un grupo de niños en situación de desplazamiento acerca de su relación con el ambiente actual y de los aspectos que la favorecen o ladesfavorecen. Es un estudio exploratorio que analiza la autopercepción de los niños participantes, su relación con los mesosistemas anteriores y actuales y el rol familiar y escolar en esta relación, con base en la observación, los mapas cognitivos, las historias de vida y las entrevistas con padres y profesores. No todos los niños consideran el desplazamiento y el proceso adaptativo negativamente. Concluimos que no es posible generalizar la forma usualmente negativa, como los niños asumen su desplazamiento y su relación con los nuevos entornos.Este artículo describe la percepción organizada por un grupo de niños en situación de desplazamiento acerca de su relación con el ambiente actual y de los aspectos que la favorecen o la desfavorecen. Es un estudio exploratorio que analiza la autopercepción de los niños participantes, su relación con los mesosistemas anteriores y actuales y el rol familiar y escolar en esta relación, conbase en la observación, los mapas cognitivos, las historias de vida y las entrevistas con padres y profesores. No todos los niños consideran el desplazamiento y el proceso adaptativo negativamente. Concluimos que no es posible generalizar la forma usualmente negativa, como los niños asumen sudesplazamiento y su relación con los nuevos entornos.This article describes the perception of a group of displaced children concerning their relationship to their current environment and the factors that favor or disfavor it. This is an exploratory study that analyzes the participating children’s self-perception, their relationship to their previous and current environments and the roles that family and school play in this relationship, taking intoaccount our observations, cognitive maps, life stories and interviews with their parents and teachers. Not all children consider homelessness and the adaptation process as negative factors. Finally, we conclude that it is not possible to set a fixed attitude for children with respect to their homelessness and their new social context

Contribución al conocimiento de la morfología y taxonomía de un hongo del genero prototrichia aislado de un cultivo de rosas

Se estudio la morfología tanto macro como microscópica de un hongo aislado de plantas de rosas. Se encontró la fructificacion correspondiente a la forma asexual típica de la especie del género Prototrichia, no se conoce su papel ecológico, sinembargo podría afectar la presentación adecuada y para la aceptación a nivel comercial de las rosas. A partir de las muestras colectadas se realizaron estudios para su clasificación. Revisada la literatura pertinente se ubico dentro del genero Prototrichia de la clase Mixomycetes, pero no dentro de alguna especie en particular, por no disponerse de momento de las ayudas necesarias para tal fin; de este hongo no se tienen registros para el neotropico (Según Farr, M. L., 1976).Se siguieron las siguientes técnicas: Siembra de esporas (Espora a Espora), en medios de Agar - avena; siembra en lámina impregnada de material nutritivo para asegurar el traspaso y germinación de las esporas y desarrollo del hongo para su descripción morfológica microscópica, y se hicieron montajes en laminas de las estructuras a estudiar para la descripción microscópica.The macroscopic and microscopic morfology of an isolated fungus of rose cultivation were studied. It to find in fructification tipic form by half direct observation of macroscopic reproduction structure, this factor assign high stetic inconvenience for products commercial presentation and accept. After a review of the taxonomic literature, we placed it in the Prototrichia genus, of class mixomycetes, but not a particular species, for absence of necesary support to moment. We used the spore to spore technique add the nutritions cover-glass technique to secure the spore transfer and germination, and fungus development for the morfologic description; and by mounting in cover-glass of microscopic structure to study

Morfología y clasificación de los hongos

Ilustraciones, fotografías y glosarioEl estudio de los hongos en toda su magnitud, requiere de una apreciación amplia e integral que conduzca a la comprensión de su biología y sus relaciones con otros seres vivos. Por lo tanto, es fundamental conocer la morfología, manejo y control para obtener diagnósticos veraces de los signos patológicos y para mostrar los beneficios y utilidades de las diferentes especies del reino Fungi. Por estas razones, nos permitimos entregar este libro con el deseo que sea consultado por aquellos profesionales interesados en el tema; así mismo proporcionar al lector una mejor apreciación de los aspectos más importantes de los hongos y sus relaciones con otros seres vivos. (Texto tomado de la fuente).Primera edició

Creencias religiosas de los estudiantes de la Universidad del Valle, Cali.

La investigación sobre las creencias religiosas de los estudiantes de la Universidad del Valle, de ambas sedes de Cali, puso a prueba las hipótesis con las que habíamos emprendido la indagación. Habíamos estudiado las tesis de la secularización de las sociedades contemporáneas y de la pluralización religiosa de la sociedad colombiana y nos habíamos formado un juicio previo acerca de la religiosidad de los estudiantes. Suponíamos que, por tratarse de una universidad pública y laica, la cantidad de estudiantes creyentes sería mínima. Encontramos que no era así, que el 83,64% de los estudiantes tienen creencias religiosas o espirituales

Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)

© 2021 The Author(s).The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients.This project was supported by the AECC (GC16173697BIGA); ISCIII (PI19/01828) co-funded by ERDF/ESF "A way to make Europe"/ "Investing in your future", CERCA/Generalitat de Catalunya SGR 2017 288 (GRC)/ “La Caixa” P. Barba was supported by the Instituto de Salud Carlos III FIS16/01433 and PERIS 2018-2020 from Generalitat de Catalunya (BDNS357800)

Adverse prognostic impact of complex karyotype (≥3 cytogenetic alterations) in adult T-cell acute lymphoblastic leukemia (T-ALL)

The potential prognostic value of conventional karyotyping in adult T-cell acute lymphoblastic leukemia (T-ALL) remains an open question. We hypothesized that a modified cytogenetic classification, based on the number and type of cytogenetic abnormalities, would allow the identification of high-risk adult T-ALL patients. Complex karyotype defined by the presence of ≥3 cytogenetic alterations identified T-ALL patients with poor prognosis in this study. Karyotypes with ≥3 abnormalities accounted for 16 % (22/139) of all evaluable karyotypes, corresponding to the largest poor prognosis cytogenetic subgroup of T-ALL identified so far. Patients carrying karyotypes with ≥3 cytogenetic alterations showed a significantly inferior response to therapy, and a poor outcome in terms of event-free survival (EFS), overall survival (OS) and cumulative incidence of relapse (CIR), independently of other baseline characteristics and the end-induction minimal residual disease (MRD) level. Additional molecular analyses of patients carrying ≥3 cytogenetic alterations showed a unique molecular profile that could contribute to understand the underlying molecular mechanisms of resistance and to evaluate novel targeted therapies (e.g. IL7R directed) with potential impact on outcome of adult T-ALL patients

Outcomes and prognostic factors of adults with refractory or relapsed T-cell acute lymphoblastic leukemia included in measurable residual disease-oriented trials

Despite high complete remission (CR) rates with frontline therapy, relapses are frequent in adults with T-cell acute lymphoblastic leukemia (T-ALL) with limited salvage options. We analyzed the outcomes and prognostic factors for CR to salvage therapy and overall survival (OS) of patients with R/R T-ALL included in two prospective measurable residual disease-oriented trials. Seventy-five patients (70 relapsed, 5 refractory) were identified. Relapses occurred in bone marrow, isolated or combined in 50 patients, and in the central nervous system (CNS; isolated or combined) in 20. Second CR was attained in 30/75 patients (40%). Treatment with FLAG-Ida and isolated CNS relapse were independently associated with a higher CR rate after first salvage therapy. The median OS was 6.2 (95% confidence interval [CI], 3.9–8.6) months, with a 4-year OS probability of 18% (95% CI, 9%–27%). No differences in survival were observed according to the treatment with hematopoietic stem cell transplantation in patients in CR after first salvage therapy. Multivariable analysis showed a ≥12-month interval between first CR and relapse, CR after first salvage therapy and isolated CNS relapse as favorable prognostic factors for OS with hazard ratios (HR) (95% CI) of 1.931 (1.109–3.362), 2.958 (1.640–5.334), and 2.976 (1.157–7.655), respectively. This study confirms the poor outcomes of adults with R/R T-ALL among whom FLAG-Ida was the best of the rescue therapies evaluated. Late relapse, CR after first rescue therapy and isolated CNS relapse showed prognostic impact on survival. More effective rescue therapies are needed in adults with R/R T-ALL.La Caixa" Foundation and ISCIII, Grant/ Award Number: PI19/01828; Generalitat de Catalunya (GRC), Grant/Award Number: 2017 SGR28

All-cause mortality in the cohorts of the Spanish AIDS Research Network (RIS) compared with the general population: 1997Ł2010

Abstract Background: Combination antiretroviral therapy (cART) has produced significant changes in mortality of HIVinfected persons. Our objective was to estimate mortality rates, standardized mortality ratios and excess mortality rates of cohorts of the AIDS Research Network (RIS) (CoRIS-MD and CoRIS) compared to the general population. Methods: We analysed data of CoRIS-MD and CoRIS cohorts from 1997 to 2010. We calculated: (i) all-cause mortality rates, (ii) standardized mortality ratio (SMR) and (iii) excess mortality rates for both cohort for 100 personyears (py) of follow-up, comparing all-cause mortality with that of the general population of similar age and gender. Results: Between 1997 and 2010, 8,214 HIV positive subjects were included, 2,453 (29.9%) in CoRIS-MD and 5,761 (70.1%) in CoRIS and 294 deaths were registered. All-cause mortality rate was 1.02 (95% CI 0.91-1.15) per 100 py, SMR was 6.8 (95% CI 5.9-7.9) and excess mortality rate was 0.8 (95% CI 0.7-0.9) per 100 py. Mortality was higher in patients with AIDS, hepatitis C virus (HCV) co-infection, and those from CoRIS-MD cohort (1997. Conclusion: Mortality among HIV-positive persons remains higher than that of the general population of similar age and sex, with significant differences depending on the history of AIDS or HCV coinfection