Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase

The membrane domain of eukaryotic HMG-CoA reductase (HMGR) has the conserved capacity to induce endoplasmic reticulum (ER) proliferation and membrane association into Organized Smooth Endoplasmic Reticulum (OSER) structures. These formations develop in response to overexpression of particular proteins, but also occur naturally in cells of the three eukaryotic kingdoms. Here, we characterize OSER structures induced by the membrane domain of Arabidopsis HMGR (1S domain). Immunochemical confocal and electron microscopy studies demonstrate that the 1S:GFP chimera co-localizes with high levels of endogenous HMGR in several ER compartments, such as the ER network, the nuclear envelope, the outer and internal membranes of HMGR vesicles and the OSER structures, which we name ER-HMGR domains. After highpressure freezing, ER-HMGR domains show typical crystalloid, whorled and lamellar ultrastructural patterns, but with wide heterogeneous luminal spaces, indicating that the native OSER is looser and more flexible than previously reported. The formation of ER-HMGR domains is reversible. OSER structures grow by incorporation of ER membranes on their periphery and progressive compaction to the inside. The ER-HMGR domains are highly dynamic in their formation versus their disassembly, their variable spherical-ovoid shape, their fluctuating borders and their rapid intracellular movement, indicating that they are not mere ER membrane aggregates, but active components of the eukaryotic cell

Beneficios del contacto precoz en madres atendidas en el Centro de Salud Justicia Paz y Vida - El Tambo, 2022

La presente investigación tuvo como problema general: ¿Qué beneficios genera el contacto precoz en madres atendidas en el Centro de Salud Justicia Paz y Vida, El Tambo?; consecuentemente, se planteó el objetivo general: Identificar los beneficios del contacto precoz en madres atendidas en el Centro de Salud Justicia Paz y Vida, El Tambo. Como fundamentos metodológicos aplicó el enfoque es cuantitativo, como un método general el método científico, de tipo básica, fue de nivel descriptivo y diseño no experimental, La población estuvo conformada por 190 mujeres atendidas de parto eutócico, según el Centro de Salud de Justicia Paz y Vida. Se aplicó la muestra probabilística, siendo los elementos de la muestra 185 mujeres a quienes se les aplicó un cuestionario. Como resultado el estudio concluyó que el contacto precoz genera un disfrute de la madre (92,94%) además de una satisfacción (66,47%). Se tuvo una prevalencia que el contacto precoz calmó el llanto del bebé (63,8%), generó como reacción primaria las caricias (66,5%) y un 88,1% sintió una emoción al tener en brazos a su bebé. Asimismo, el contacto precoz generó que el bebé agarró y succionó el pezón (61,6%) y un 90,8% que tienen lactancia materna exclusiva a la semana del parto. Como conclusión: se llegó a que, si existen beneficios al contacto precoz en la madre como en el recién nacido, con respecto a la satisfacción, adaptación, el vínculo y el fortalecimiento de la lactancia materna. Se recomienda: brindar información y realizar sesiones educativas a las madre

Condiciones oceanograficas frente a las costas del Peru en mayo 2000

Se analizan las condiciones oceanográficas observadas durante el III Crucero Regional Conjunto de Investigación Oceanografía en el Pacifico Sudeste, Perú, realizado a bordo de los BICs Humboldt (12 - 25 mayo) y SNP-2 (15 mayo - I junio 2000).13 p.IMARP

Modelo de la teoría de colas en el área de servicios al contribuyente de la SUNAT - Trujillo para disminuir el tiempo de atención al contribuyente

La presente investigación tiene como objetivo que a través de la aplicación de teoría de colas establecer el número óptimo de ventanillas disponibles que permita minimizar los tiempos de atención para ser atendidos, con la finalidad de lograr una mayor satisfacción de los contribuyentes que asisten a la SUNAT de la ciudad de Trujillo. El proyecto estuvo enmarcado en el tipo de investigación aplicativa, fundamentada a nivel descriptiva y transversal con un diseño de fuente no experimental. Se empleó un instrumento para la recolección de datos, la hoja de tiempo, mediante el cual se registraron los tiempos del proceso de atención al contribuyente. Para establecer el número óptimo de ventanillas disponibles, se realizó el diagnóstico actual de las líneas de espera en el área de servicio al contribuyente, se determinó la variabilidad en los tiempos de arribo y tiempo de atención del servicio y se utilizó una herramienta tecnológica de simulación como el software QM for Windows que nos ayudó a determinar los costos para validar que el sistema aplicado es beneficioso para la empresa. De esta manera se pudo concluir que en promedio un contribuyente espera un tiempo de 00:06:09, teniendo como base 11 servidores, la cual se puedo determinar el 85 % de ahorro en el tiempo improductivo.The objective of this research is that through the application of queue theory to establish the optimal number of available windows that allow minimizing waiting times, to achieve greater satisfaction of the taxpayers who attend the SUNAT of the city from Trujillo. The project was framed in the type of applicative research, based on a descriptive and cross-sectional level with a non-experimental source design. An instrument was used for data collection, the timesheet, using which the times of the taxpayer service process were recorded. To establish the optimal number of windows available, the current diagnosis of the waiting lines in the taxpayer service area was carried out, the variability in the arrival times and service attention time was determined and a technological simulation tool was used. such as the QM software for Windows that helped us determine the costs to validate that the applied system is beneficial for the company. In this way, it was possible to conclude that on average a taxpayer waits a time of 00:06:09, based on 11 servers, which can determine 85% savings in unproductive time.Tesi

Stratus Ocean Reference Station (20˚S, 85˚W) mooring recovery and deployment cruise STRATUS 8 R/V Ronald H. Brown cruise 07-09 October 9, 2007–November 6, 2007

The Ocean Reference Station at 20°S, 85°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology (air-sea fluxes of heat, freshwater, and momentum), and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with cruises between October and December. During the October 2007 cruise on the NOAA ship Ronald H. Brown to the ORS Stratus site, the primary activities were recovery of the Stratus 7 WHOI surface mooring that had been deployed in October 2006, deployment of a new (Stratus 8) WHOI surface mooring at that site; in-situ calibration of the buoy meteorological sensors by comparison with instrumentation put on board the ship by staff of the NOAA Earth System Research Laboratory (ESRL); and observations of the stratus clouds and lower atmosphere by NOAA ESRL. Meteorological sensors on a buoy for the Pacific tsunami warning system were also serviced, in collaboration with the Hydrographic and Oceanographic Service of the Chilean Navy (SHOA). The DART (Deep-Ocean Assessment and Reporting of Tsunami) carries IMET sensors and subsurface oceanographic instruments. A new DART II buoy was deployed north of the STRATUS buoy, by personnel from the National Data Buoy Center (NDBC) Argo floats and drifters were launched, and CTD casts carried out during the cruise. The ORS Stratus buoys are equipped with two Improved Meteorological (IMET) systems, which provide surface wind speed and direction, air temperature, relative humidity, barometric pressure, incoming shortwave radiation, incoming longwave radiation, precipitation rate, and sea surface temperature. Additionally, the Stratus 8 buoy received a partial pressure of CO2 detector from the Pacific Marine Environmental Laboratory (PMEL). IMET data are made available in near real time using satellite telemetry. The mooring line carries instruments to measure ocean salinity, temperature, and currents. The ESRL instrumentation used during the 2007 cruise included cloud radar, radiosonde balloons, and sensors for mean and turbulent surface meteorology. Finally, the cruise hosted a teacher participating in NOAA’s Teacher at Sea Program.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA17RJ1223 for the Cooperative Institute for Climate and Ocean Research (CICOR)

Aplicación del método ABP para el desarrollo del pensamiento crítico de estudiantes universitarios de educación

El propósito del presente estudio fue determinar la influencia de la aplicación del método ABP en el desarrollo del pensamiento crítico de estudiantes universitarios de educación de la Universidad San Pedro Sede Barranca en el año 2016. La investigación desarrollada fue de tipo aplicada con un enfoque cuantitativo y el diseño de investigación que se desarrolló fue Pre Experimental con un grupo de trabajo. Se tomó en cuenta como población censal a 180 estudiantes de educación Inicial de la Universidad San Pedro de Barranca en el año 2016. Se utilizó como instrumento de medición un test validado por juicio de expertos y se obtuvo una fiabilidad a través de Alfa de Cronbach de 0.89. Se determinó que el nivel de pensamiento crítico de los estudiantes de Educación de la Universidad San Pedro Sede Barranca, tras la aplicación del Método ABP arrojaron que el 9,45% de estudiantes pasaron de nivel de pensamiento crítico bajo a medio; el 2, 78% de nivel bajo a alto y el 28,88% de nivel medio a alto, que sumado representa el 41, 11 del total de la población que han cambiado de nivel de pensamiento crítico, apreciándose así la influencia significativa del método Aprendizaje basado en problemas en el desarrollo del pensamiento crítico de los estudiantes, lo que resulta significativo para aceptar la hipótesis de nuestra investigación científica.Trabajo de investigació

Stratus 9/VOCALS ninth setting of the Stratus Ocean Reference Station & VOCALS Regional Experiment

The Ocean Reference Station at 20°S, 85°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology; air-sea fluxes of heat, freshwater, and momentum; and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with cruises that have come between October and December. During the 2008 cruise on the NOAA ship Ronald H. Brown to the ORS Stratus site, the primary activities were recovery of the Stratus 8 WHOI surface mooring that had been deployed in October 2007, deployment of a new (Stratus 9) WHOI surface mooring at that site; in-situ calibration of the buoy meteorological sensors by comparison with instrumentation put on board by staff of the NOAA Earth System Research Laboratory (ESRL); and observations of the stratus clouds and lower atmosphere by NOAA ESRL. A buoy for the Pacific tsunami warning system was also serviced in collaboration with the Hydrographic and Oceanographic Service of the Chilean Navy (SHOA). The DART (Deep-Ocean Assessment and Reporting of Tsunami) carries IMET sensors and subsurface oceanographic instruments. A DART II buoy was deployed north of the STRATUS buoy, by personnel from the National Data Buoy Center (NDBC) Argo floats and drifters were launched, and CTD casts carried out during the cruise. The ORS Stratus buoys are equipped with two Improved Meteorological (IMET) systems, which provide surface wind speed and direction, air temperature, relative humidity, barometric pressure, incoming shortwave radiation, incoming longwave radiation, precipitation rate, and sea surface temperature. Additionally, the Stratus 8 buoy received a partial CO2 detector from the Pacific Marine Environmental Laboratory (PMEL). IMET data are made available in near real time using satellite telemetry. The mooring line carries instruments to measure ocean salinity, temperature, and currents. The ESRL instrumentation used during the 2008 cruise included cloud radar, radiosonde balloons, and sensors for mean and turbulent surface meteorology. Finally, the cruise hosted a teacher participating in NOAA’s Teacher at Sea Program.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA17RJ1223 for the Cooperative Institute for Climate and Ocean Research (CICOR)

High incidence of clinical fragility fractures in postmenopausal women with rheumatoid arthritis. A case-control study.

Objectives: To estimate the incidence of clinical fragility fractures in postmenopausal women with rheumatoid arthritis (RA) and analyze risk factors for fracture. Methods: Incidence of clinical fragility fractures in 330 postmenopausal women with RA was compared to that of a control population of 660 age-matched postmenopausal Spanish women. Clinical fractures during the previous five years were recorded. We analyzed associations with risk factors for fracture in both populations and with disease-related variables in RA patients. Results: Median age of RA patients was 64 years; median RA duration was eight years. Sixty-nine percent were in remission or on low activity. Eighty-five percent had received glucocorticoids (GCs); 85 %, methotrexate; and 40 %, ≥1 biologic DMARD. Fifty-four patients and 47 controls had ≥1 major osteoporotic fracture (MOF). Incidence of MOFs was 3.55 per 100 patient-year in patients and 0.72 in controls (HR: 2.6). Risk factors for MOFs in RA patients were age, previous fracture, parental hip fracture, years since menopause, BMD, erosions, disease activity and disability, and cumulative dose of GCs. Previous fracture in RA patients was a strong risk for MOFs (HR: 10.37). Conclusion: Of every 100 postmenopausal Spanish women with RA, 3-4 have a MOF per year. This is more than double that of the general population. A previous fracture poses a high risk for a new fracture. Other classic risk factors for fracture, RA disease activity and disability, and the cumulative dose of GCs are associated with fracture development

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis