The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium

Research Areas: Life Sciences & Biomedicine ; MicrobiologyABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.info:eu-repo/semantics/publishedVersio

Myeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditions

Research Areas: Agriculture ; Veterinary Sciences ; ZoologyABSTRACT - Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives of this in vitro work were to investigate, in explants of mare’s endometrium, (i) the ABAH capacity to inhibit MPO-induced collagen type I (COL1) expression; and (ii) the action of MPO and ABAH on the expression and gelatinolytic activity of MMP-2/-9. Explants retrieved from the endometrium of mares in follicular or mid-luteal phases were treated with MPO, ABAH, or their combination, for 24 or 48 h. The qPCR analysis measured the transcription of COL1A2, MMP2, and MMP9. Western blot and zymography were performed to evaluate COL1 protein relative abundance and gelatinolytic activity of MMP-2/-9, respectively. Myeloperoxidase elevated COL1 relative protein abundance at both treatment times in follicular phase (p < 0.05). The capacity of ABAH to inhibit MPO-induced COL1 was detected in follicular phase at 48 h (p < 0.05). The gelatinolytic activity of activated MMP-2 augmented in mid-luteal phase at 24 h after MPO treatment, but it was reduced with MPO+ABAH treatment. The activity of MMP-9 active form augmented in MPO-treated explants. However, this effect was inhibited by ABAH in the follicular phase at 48 h (p < 0.05). By inhibiting the pro-fibrotic effects of MPO, it might be possible to reduce the development of endometrosis. Metallopeptidase-2 might be involved in an acute response to MPO in the mid-luteal phase, while MMP-9 might be implicated in a prolonged exposition to MPO in the follicular phase.info:eu-repo/semantics/publishedVersio

Temas de investigação em direitos humanos para o século XXI

Edição comemorativa do 10.º aniversário do Mestrado em Direitos Humanos da Universidade do Minho.Este livro é uma celebração do ensino e da investigação em direitos humanos que têm vindo a ser desenvolvidos, na Escola de Direito da Universidade do Minho, há já mais de uma década. A sua publicação num momento em que se avolumam os riscos para valores fundamentais subjacentes à proteção dos direitos humanos – como a igualdade e a não discriminação, a proibição da escravatura e de tratamentos cruéis, desumanos e degradantes, a liberdade de religião ou crença, entre muitos outros –, torna-o especialmente oportuno. Os sinais de aparente retrocesso no consenso das nossas sociedades a respeito desses valores – visíveis no triunfo político de discursos abertamente racistas, xenófobos, sexistas, etc. – recordam-nos que, também no mundo ocidental, os direitos humanos são um work in progress, não um dado adquirido. Os novos riscos para a dignidade da pessoa humana associados aos avanços tecnológicos andam de par com velhas formas de subalternização e de opressão. O campo para a reflexão crítica é muito vasto. Os temas que hoje (pre)ocupam académicos, decisores políticos e ativistas de direitos humanos são também aqueles que estruturam o plano de estudos do Mestrado em Direitos Humanos da Universidade do Minho. Todos estes temas surgem ao longo do presente livro, que reúne contributos de muitos dos membros da comunidade científica e académica que o Mestrado em Direitos Humanos mobilizou e ajudou a dinamizar ao longo da última década, entre docentes do Mestrado, colaboradores em júris de provas públicas e/ou na orientação de mestrandos, oradores convidados e estudantes. Os textos aqui reunidos refletem bem as sinergias interdisciplinares, interinstitucionais e inter-nacionais que o Mestrado em Direitos Humanos foi capaz de criar, não apenas pela variedade de campos disciplinares representados – Direito, Filosofia, Relações Internacionais, Antropologia –, mas também pela participação de autores que são docentes e/ou investigadores em diversas instituições nacionais e estrangeiras, como a Faculdade de Direito da Universidade do Porto, a Faculdade de Economia da Universidade de Coimbra, a Universidade Federal da Paraíba e a Pontifícia Universidade Católica de São Paulo.info:eu-repo/semantics/publishedVersio

Realização de cardiomiotomia a heller-pinotti em paciente com megaesôfago grau ii: Relato de acalásia idiopática / Heller-pinotti cardiomyotomy in a patient with grade ii megaesophagus: Report of idiopathic acalasia

Objetivo: relatar um caso de um paciente com acalásia idiopática grau II, de acordo com a Classificação de Chicago, que foi submetido a uma cardiomiotomia a heller-pinotti. Relato de caso: paciente de 17 anos, masculino, com história de disfagia, com dificuldade na deglutição de alimentos sólidos e pastosos em progressão. Realizados exames subsidiários como esofagograma baritado e manometria esofágica de alta resolução para confirmação do diagnóstico. Considerações finais: casos de acalásias são pouco frequentes, porém o diagnóstico precoce e condutas adequadas ajudam na boa evolução do paciente. Um dos métodos aplicados na correção com excelentes resultados é a técnica de Heller-Pinotti por videolaparoscopia

Congresso Internacional “Diálogos Interculturais Portugal-China”: livro de resumos

O Instituto Confúcio da Universidade de Aveiro (IC-UA), em parceria com vários Departamentos da Universidade de Aveiro (DLC, DeCA, DCSPT, DEGEIT), organiza, nos dias 15, 16 e 17 de fevereiro de 2017, o Congresso Internacional “Diálogos Interculturais Portugal-China”. Do programa do congresso constam conferências plenárias, mesas redondas, comunicações livres, pósteres e exposições. (...

Tipologia de rios em Portugal Continental no âmbito da implementação da Directiva Quadro da Água. I - Caracterização abiótica.

Os tipos são grupos de massas de água com características geográficas e hidrológicas relativamente homogéneas, consideradas relevantes para a determinação das condições ecológicas. O objectivo da definição de tipos é permitir que sejam correctamente estabelecidas condições de referência e que sejam comparáveis as classificações de estado ecológico dentro de cada grupo de rios com características semelhantes. Na definição da Tipologia para Rios, procedeu-se inicialmente à aplicação do sistema A (Anexo II, DQA) tendo-se obtido 18 tipos. Estando a generalidade dos tipos representada em todo o território continental, considerou-se que este sistema não traduziria a heterogeneidade ecológica existente, não reflectindo o gradiente climático Norte – Sul, particularmente evidente para a temperatura e precipitação. Nesse sentido entendeu-se necessário aplicar o Sistema B (Anexo II, DQA)

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio

Networking and Participatory Research Promoting Quality of Life and Well-Being in Portuguese-Speaking African Countries

Spread across the planet each human being, individually or in community, aspires for well-being and quality of life, according to the ideal of each one. However, we all believe that there are always ways to live better. For many people the measurement of a better life translates into the guarantee of social rights, the right to basic services, good land, seed and sufficient nutritious food for their community members. The Mechanism to Facilitate the Participation of Universities in the Food and Nutrition Security Council of the Community of Portuguese Speaking Countries is a cooperative academic network fomented by the Community of Portuguese Speaking Countries (CPLP) with support from the Food and Agriculture Organization of the United Nations. This mechanism works with teaching, research and extension in the CPLP Food and Nutrition Security Strategy. The pillars of CPLP Strategy are the strengthening of the governance of public policies on Food and Nutrition Security at all levels of government, social protection based on guaranteeing access to food and family farming with a strategy to increase the availability of good quality food, promoting social and environmental sustainability. CPLP University Mechanism has provided training processes for technicians who work in public policies for Food and Nutrition Security and has contributed to the strengthening of postgraduate programs in Portuguese-speaking African countries. As consequence, it has favored participatory research and mixed methods as a theoretical methodological approach. Therefore, it seeks to focus on the territories of Food and Nutrition Security practices to transform reality, as recommended by CPLP Strategy, however, with the autonomous assumptions of the collaborative network. This chapter presents how local researchers perceive the results of a process of inducing an academic network to transform the local reality and promote Food and Nutrition Security in the context of the CPLP

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio