331 research outputs found
VALIDATING GLOBAL STRUCTURAL DAMPING MODELS FOR DYNAMIC ANALYSES
Abstract: Finite Element (FE) models grow in terms of detail and complexity. They strive to provide a more precise mass and stiffness distribution in order to achieve better load prediction capabilities. However, they also need to include damping models to achieve better results for dynamic loads analyses. This is why experiments are usually carried out to quantify global damping ratios of the final structure and include them in the analytical model for further calculations. Yet, especially for large aerospace structures assembled from different substructures, the experimental determination of damping ratios for the assembled structure may be impossible or ineconomical. Therefore, a consistent approach to predict the damping properties of assembled structures is desirable. In this work, FE models of a laboratory test structure and its two substructures are built up. Modal tests are carried out on the substructures. On the basis of correlated substructure modal damping ratios, global proportional damping models are applied on substructure level in order to build proportional substructure damping matrices, construct a nonproportional, full structure damping matrix and thus predict the damping properties for the fully assembled structure. The approach is validated with the help of experimental results from a modal test on the fully assembled laboratory test structure. Because of the unsatisfactory reproduction of the substructure damping properties by the selected damping models and the outcome for the assembled structure in this work, an additional investigation on computational model updating of damping parameters on substructure level is carried out on a simulated plate
A multivariate interval approach for inverse uncertainty quantification with limited experimental data
© 2018 Elsevier Ltd This paper introduces an improved version of a novel inverse approach for the quantification of multivariate interval uncertainty for high dimensional models under scarce data availability. Furthermore, a conceptual and practical comparison of the method with the well-established probabilistic framework of Bayesian model updating via Transitional Markov Chain Monte Carlo is presented in the context of the DLR-AIRMOD test structure. First, it is shown that the proposed improvements of the inverse method alleviate the curse of dimensionality of the method with a factor up to 105. Furthermore, the comparison with the Bayesian results revealed that the selection ofthe most appropriate method depends largely on the desired information and availability of data. In case large amounts of data are available, and/or the analyst desires full (joint)-probabilistic descriptors of the model parameter uncertainty, the Bayesian method is shown to be the most performing. On the other hand however, when such descriptors are not needed (e.g., for worst-case analysis), and only scarce data are available, the interval method is shown to deliver more objective and robust bounds on the uncertain parameters. Finally, also suggestions to aid the analyst in selecting the most appropriate method for inverse uncertainty quantification are given
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Comparative prebiotic activity of mixtures of cereal grain polysaccharides
The main components of the non-starch polysaccharide (NSP) fraction of wheat flour are arabinoxylan (AX) and β-glucan. These are also present in other cereal grains, but their proportions vary with AX being the major component in wheat and rye and β-glucan in barley and oats. Therefore, it was hypothesised that these NSPs could act synergistically when fermented in vitro at the ratios present in the major foods consumed, resulting in increased prebiotic activity. AX and β-glucan were therefore tested in in vitro fermentation studies to assess their prebiotic activity when used individually and/or in different ratios. Short-chain fatty-acids (SCFAs) produced from in vitro fermentation were measured using HPLC and bacterial populations were measured using flow cytometry with fluorescence in situ hybridisation (Flow-FISH). Fermentation of AX alone resulted in a significant bifidogenic activity and increased concentrations of SCFAs, mainly acetate, after 8-24 h of fermentation, however β-glucan alone did not show prebiotic activity. The greatest prebiotic activity, based on concentration of total SCFAs and increases in total bacteria as well as beneficial Bifidobacterium and Clostridium coccoides/Eubacterium groups, was observed when AX and β-glucan were combined at a 3:1 ratio, which corresponds to their ratios in wheat flour which is major source of cereal fibre in the diet. This indicates that the population of bacteria in the human GI tract may be modulated by the composition of the fibre in the diet, to maximise the prebiotic potential
A non-destructive method to determine the neutron production rate of a sample of spent nuclear fuel under standard controlled area conditions
A method to determine the neutron production rate of a sample of spent nuclear fuel by means of non-destructive analysis conducted under controlled-area conditions is described, validated and demonstrated. A standard neutron well-counter designed for routine nuclear safeguards applications is applied. The method relies on a transfer procedure that is adapted to the hot-cell facilities at the laboratory for high and medium level activity of the SCK CEN. The sample transfer and measurement procedures are described together with results of Monte Carlo simulations. Experiments with radionuclide sources were carried out at the Joint Research Centre to test the procedures and to determine the performance characteristics of the detection device. Finally, measurements of a segment of a spent nuclear fuel rod were carried out at the SCK CEN to validate and demonstrate the method.JRC.G.2-Standards for Nuclear Safety, Security and Safeguard
Focused Fluid Flow along the Nootka Fault Zone and Continental slope, Explorer‐Juan de Fuca Plate Boundary
Key Points:
- Fluid flow is focused along Nootka Fault traces resulting in shallow bright spots
- Two seafloor mounds are the result of basaltic intrusions in the Nootka Fault zone
- Gas hydrates occur at the Nootka Slope and are imaged seismically as bottom-
simulating reflectors suggesting a regional heat-flow of ~80 mW/m2 along the slope
Abstract
Geophysical and geochemical data indicate there is abundant fluid expulsion in the Nootka fault zone (NFZ) between the Juan de Fuca and Explorer plates and the Nootka continental slope. Here we combine observations from > 20 years of investigations to demonstrate the nature of fluid‐flow along the NFZ, which is the seismically most active region off Vancouver Island. Seismicity reaching down to the upper mantle is linked to near‐seafloor manifestation of fluid flow through a network of faults. Along the two main fault traces, seismic reflection data imaged bright spots 100 – 300 m below seafloor that lie above changes in basement topography. The bright spots are conformable to sediment layering, show opposite‐to‐seafloor reflection polarity, and are associated with frequency‐reduction and velocity push‐down indicating the presence of gas in the sediments. Two seafloor mounds ~15 km seaward of the Nootka slope are underlain by deep, non‐conformable high amplitude reflective zones. Measurements in the water column above one mound revealed a plume of warm water, and bottom‐video observations imaged hydrothermal vent system biota. Pore fluids from a core at this mound contain predominately microbial methane (C1) with a high proportion of ethane (C2) yielding C1/C2 ratios < 500 indicating a possible slight contribution from a deep source. We infer the reflective zones beneath the two mounds are basaltic intrusions that create hydrothermal circulation within the overlying sediments. Across the Nootka continental slope, gas hydrate related bottom‐simulating reflectors are widespread and occur at depths indicating heat‐flow values of 80 – 90 mW/m2
An absolute measurement of the neutron production rate of a spent nuclear fuel sample used for depletion code validation
A method to determine the neutron production rate of a spent nuclear fuel segment sample by means of non-destructive assay conducted under standard controlled-area conditions is described and demonstrated. A neutron well counter designed for routine nuclear safeguards applications is applied. The method relies on a transfer procedure that is adapted to the hot cell facilities at the Laboratory for High and Medium level Activity of SCK CEN in Belgium. Experiments with 252Cf(sf) sources, certified for their neutron emission rate, were carried out at the Joint Research Centre to determine the characteristics of the detection device. Measurements of a segment of a spent nuclear fuel rod were carried out at SCK CEN resulting in an absolute and non-destructive measurement of the neutron production rate avoiding any reference to a representative spent nuclear fuel sample to calibrate the device. Results of these measurements were used to study the performance of depletion codes, i.e., ALEPH2, SCALE, and Serpent2. The study includes a code-to-code and code-to-experiment comparison using different nuclear data libraries
The Lectin Receptor Kinase LecRK-I.9 Is a Novel Phytophthora Resistance Component and a Potential Host Target for a RXLR Effector
In plants, an active defense against biotrophic pathogens is dependent on a functional continuum between the cell wall (CW) and the plasma membrane (PM). It is thus anticipated that proteins maintaining this continuum also function in defense. The legume-like lectin receptor kinase LecRK-I.9 is a putative mediator of CW-PM adhesions in Arabidopsis and is known to bind in vitro to the Phytophthora infestans RXLR-dEER effector IPI-O via a RGD cell attachment motif present in IPI-O. Here we show that LecRK-I.9 is associated with the plasma membrane, and that two T-DNA insertions lines deficient in LecRK-I.9 (lecrk-I.9) have a ‘gain-of-susceptibility’ phenotype specifically towards the oomycete Phytophthora brassicae. Accordingly, overexpression of LecRK-I.9 leads to enhanced resistance to P. brassicae. A similar ‘gain-of-susceptibility’ phenotype was observed in transgenic Arabidopsis lines expressing ipiO (35S-ipiO1). This phenocopy behavior was also observed with respect to other defense-related functions; lecrk-I.9 and 35S-ipiO1 were both disturbed in pathogen- and MAMP-triggered callose deposition. By site-directed mutagenesis, we demonstrated that the RGD cell attachment motif in IPI-O is not only essential for disrupting the CW-PM adhesions, but also for disease suppression. These results suggest that destabilizing the CW-PM continuum is one of the tactics used by Phytophthora to promote infection. As countermeasure the host may want to strengthen CW-PM adhesions and the novel Phytophthora resistance component LecRK-I.9 seems to function in this process
Effects of the lactase 13910 C/T and calcium-sensor receptor A986S G/T gene polymorphisms on the incidence and recurrence of colorectal cancer in Hungarian population
Background: Epidemiological studies suggested the chemopreventive role of higher calcium intake in colorectal carcinogenesis. We examined genetic polymorphisms that might influence calcium metabolism: lactase (LCT) gene 13910 C/T polymorphism causing lactose intolerance and calcium-sensing receptor (CaSR) gene A986S polymorphism as a responsible factor for the altered cellular calcium sensation. Methods: 538 Hungarian subjects were studied: 278 patients with colorectal cancer and 260 healthy controls. Median follow-up was 17 months. After genotyping, the relationship between LCT 13910 C/T and CaSR A986S polymorphisms as well as tumor incidence/progression was investigated. Results: in patient with colorectal cancer, a significantly higher LCT CC frequency was associated with increased distant disease recurrence (OR = 4.04; 95% CI = 1.71-9.58; p = 0.006). The disease free survival calculated from distant recurrence was reduced for those with LCT CC genotype (log rank test p = 0.008). In case of CaSR A986S polymorphism, the homozygous SS genotype was more frequent in patients than in controls (OR = 4.01; 95% CI = 1.33-12.07; p = 0.014). The number of LCT C and CaSR S risk alleles were correlated with tumor incidence (p = 0.035). The CCSS genotype combination was found only in patients with CRC (p = 0.033). Conclusion: LCT 13910 C/T and CaSR A986S polymorphisms may have an impact on the progression and/or incidence of CRC
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