In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

Background In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we explore the potential of plant autofluorescence (specifically chlorophyll fluorescence) to provide a readout of herbicide action across the scales utilising multiphoton-excited fluorescence lifetime imaging, wide-field single-photon excited fluorescence lifetime imaging and single point fluorescence lifetime measurements via a fibre-optic probe. Results Our studies indicate that changes in chlorophyll fluorescence lifetime can be utilised as an indirect readout of a photosystem II inhibiting herbicide activity in living plant leaves at three different scales: cellular (~μm), single point (~1 mm2) and macroscopic (~8 × 6 mm2 of a leaf). Multiphoton excited fluorescence lifetime imaging of Triticum aestivum leaves indicated that there is an increase in the spatially averaged chlorophyll fluorescence lifetime of leaves treated with Flagon EC—a photosystem II inhibiting herbicide. The untreated leaf exhibited an average lifetime of 560 ± 30 ps while the leaf imaged 2 h post treatment exhibited an increased lifetime of 2000 ± 440 ps in different fields of view. The results from in vivo wide-field single-photon excited fluorescence lifetime imaging excited at 440 nm indicated an increase in chlorophyll fluorescence lifetime from 521 ps in an untreated leaf to 1000 ps, just 3 min after treating the same leaf with Flagon EC, and to 2150 ps after 27 min. In vivo single point fluorescence lifetime measurements demonstrated a similar increase in chlorophyll fluorescence lifetime. Untreated leaf presented a fluorescence lifetime of 435 ps in the 440 nm excited chlorophyll channel, CH4 (620–710 nm). In the first 5 min after treatment, mean fluorescence lifetime is observed to have increased to 1 ns and then to 1.3 ns after 60 min. For all these in vivo plant autofluorescence lifetime measurements, the plants were not dark-adapted. Conclusions We demonstrate that the local impact of a photosystem II herbicide on living plant leaves can be conveniently mapped in space and time via changes in autofluorescence lifetime, which we attribute to changes in chlorophyll fluorescence. Using portable fibre-optic probe instrumentation originally designed for label-free biomedical applications, this capability could be deployed outside the laboratory for monitoring the distribution of herbicides in growing plants

Accelerating Growth and Size-dependent Distribution of Human Activities Online

Research on human online activities usually assumes that total activity $T$ increases linearly with active population $P$, that is, $T\propto P^{\gamma}(\gamma=1)$. However, we find examples of systems where total activity grows faster than active population. Our study shows that the power law relationship $T\propto P^{\gamma}(\gamma>1)$ is in fact ubiquitous in online activities such as micro-blogging, news voting and photo tagging. We call the pattern "accelerating growth" and find it relates to a type of distribution that changes with system size. We show both analytically and empirically how the growth rate $\gamma$ associates with a scaling parameter $b$ in the size-dependent distribution. As most previous studies explain accelerating growth by power law distribution, the model of size-dependent distribution is novel and worth further exploration.Comment: 6 pages, 2 figure

DDR1 autophosphorylation is a result of aggregation into dense clusters

The collagen receptor DDR1 is a receptor tyrosine kinase that promotes progression ofa wide range of human disorders. Little is known about how ligand binding triggers DDR1 kinase activity. We previously reported that collagen induces DDR1 activation through lateral dimer association and phosphorylation between dimers, a process that requires specific transmembrane association. Here we demonstrate ligand-induced DDR1 clustering by widefield and super-resolution imaging and provide evidence for a mechanism whereby DDR1 kinase activity is determined by its molecular density. Ligand binding resulted in initial DDR1 reorganisation into morphologically distinct clusters with unphosphorylated DDR1. Further compaction over time led to clusters with highly aggregated and phosphorylated DDR1. Ligand-induced DDR1 clustering was abolished by transmembrane mutations but did not require kinase activity. Our results significantly advance our understanding of the molecular events underpinning ligand-induced DDR1 kinase activity and provide an explanation for the unusually slow DDR1 activation kinetics

One-dimensional non-interacting fermions in harmonic confinement: equilibrium and dynamical properties

We consider a system of one-dimensional non-interacting fermions in external harmonic confinement. Using an efficient Green's function method we evaluate the exact profiles and the pair correlation function, showing a direct signature of the Fermi statistics and of the single quantum-level occupancy. We also study the dynamical properties of the gas, obtaining the spectrum both in the collisionless and in the collisional regime. Our results apply as well to describe a one-dimensional Bose gas with point-like hard-core interactions.Comment: 11 pages, 5 figure

"Supersolid" self-bound Bose condensates via laser-induced interatomic forces

We show that the dipole-dipole interatomic forces induced by a single off-resonant running laser beam can lead to a self-bound pencil-shaped Bose condensate, even if the laser beam is a plane-wave. For an appropriate laser intensity the ground state has a quasi-one dimensional density modulation --- a Bose "supersolid".Comment: 4 pages, 3 eps figure

Echo in Optical Lattices: Stimulated Revival of Breathing Oscillations

We analyze a stimulated revival (echo) effect for the breathing modes of the atomic oscillations in optical lattices. The effect arises from the dephasing due to the weak anharmonicity being partly reversed in time by means of additional parametric excitation of the optical lattice. The shape of the echo response is obtained by numerically simulating the equation of motion for the atoms with subsequent averaging over the thermal initial conditions. A qualitative analysis of the phenomenon shows that the suggested echo mechanism combines the features of both spin and phonon echoes.Comment: 13 pages, 3 figure

Mean field effects in a trapped classical gas

In this article, we investigate mean field effects for a bosonic gas harmonically trapped above the transition temperature in the collisionless regime. We point out that those effects can play also a role in low dimensional system. Our treatment relies on the Boltzmann equation with the inclusion of the mean field term. The equilibrium state is first discussed. The dispersion relation for collective oscillations (monopole, quadrupole, dipole modes) is then derived. In particular, our treatment gives the frequency of the monopole mode in an isotropic and harmonic trap in the presence of mean field in all dimensions.Comment: 4 pages, no figure submitted to Phys. Rev.

Radiation damping optical enhancement in cold atoms

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/Open Access journalThe typically tiny effect of radiation damping on a moving body can be amplified to a favorable extent by exploiting the sharp reflectivity slope at one edge of an optically induced stop-band in atoms loaded into an optical lattice. In this paper, this phenomenon is demonstrated for the periodically trapped and coherently driven cold 87Rb atoms, where radiation damping might be much larger than that anticipated in previous proposals and become comparable with radiation pressure. Such an enhancement could be observed even at speeds of only a few meters per second with less than 1.0% absorption, making radiation damping experimentally accessible

Resonant nonlinear magneto-optical effects in atoms

In this article, we review the history, current status, physical mechanisms, experimental methods, and applications of nonlinear magneto-optical effects in atomic vapors. We begin by describing the pioneering work of Macaluso and Corbino over a century ago on linear magneto-optical effects (in which the properties of the medium do not depend on the light power) in the vicinity of atomic resonances, and contrast these effects with various nonlinear magneto-optical phenomena that have been studied both theoretically and experimentally since the late 1960s. In recent years, the field of nonlinear magneto-optics has experienced a revival of interest that has led to a number of developments, including the observation of ultra-narrow (1-Hz) magneto-optical resonances, applications in sensitive magnetometry, nonlinear magneto-optical tomography, and the possibility of a search for parity- and time-reversal-invariance violation in atoms.Comment: 51 pages, 23 figures, to appear in Rev. Mod. Phys. in Oct. 2002, Figure added, typos corrected, text edited for clarit

Visible Raman-shifted Fiber Lasers for Biophotonic Applications

The efficient nonlinear conversion of Yb-doped fiber laser systems using a combination of stimulated Raman scattering and second-harmonic generation is an effective method for developing sources for biophotonic applications in the yellow-green spectral region. In this paper, we review recent progress in the development of these sources, compare the relative benefits of differing source architectures and demonstrate STED microscopy using an exemplar source