DESAIN DAN IMPLEMENTASI MODUL PEMBANGKIT LISTRIK MIKROHIDRO PADA RUMAH DENGAN POMPA PENYIMPANAN ENERGI

DESAIN DAN IMPLEMENTASI MODUL PEMBANGKIT LISTRIK MIKROHIDRO PADA RUMAH DENGAN POMPA PENYIMPANAN ENERGI Pembangkit Listrik Tenaga Air (PLTA) adalah energi listrik yang dihasilkan dengan menggunakan energi air yang bergerak mengubah energi potensial air menjadi energi listrik. Turbin air digunakan untuk mengubah energi potensial air menjadi kerja mekanis poros yang akan memutar generator untuk menghasilkan energi listrik. Dalam Tugas Akhir kali ini telah dilakukan perancangan dan implementasi Pembangkit listrik Micro Hydro Pumped Storage dengan metode pembangkitan energi yang identik dengan hydroelectric plant pada sebuah Solar Home yang digunakan sebagai penyimpan daya bantuan. Penyimpanan air dilakukan dengan cara memompa air dari tempat penyimpanan air yang lebih rendah menuju tempat penyimpanan air yang berada ditempat yang lebih tinggi menggunakan daya berlebih yang dihasilkan oleh PV. Pada saat beban listrik sebuah rumah terlalu besar atau baterai tidak sanggup untuk menyuplai daya yang cukup ke beban, maka Pumped Storage akan membuka keran sehingga air dapat mengalir dari tempat penyimpanan yang tinggi menuju tempat penyimpanan yang lebih rendah sehingga dapat menjadi sebuah pembangkit listrik tenaga air yang digunakan untuk menghasilkan daya listrik bantuan. Kata kunci: pumped storage , Pembangkit Listrik Tenaga Air, HydroElectric plant, Energy Storage

PROGRAM LOYALITAS DENGAN A CARD FLAZZ PADA MINIMARKET ALFAMART

The competition among the retailers cause retailers build customer loyalty. One of the way to build it is by applied loyalty program. The loyalty program have the influenced towards the relationship between retailer and customer. The main question is identify the type-type of loyalty program that given Alfamart Minimarket, and identify the impact of loyalty program toward customer loyalty at Alfamart Minimarket. There are the various loyalty program for customer, and its showing that the retailer care to customers. By the memberships cards can build the retailer community. The interesting rewards to customer can build the positive respond. The succesfully Alfamart to reach the Superbrand award at 2010, 2011, and 2012 proved that the loyalty program have influenced towards image and implied towards the buying behavior

Software Development Dengan Extreme Programming (XP) Pada Aplikasi Deteksi Kemiripan Judul Skripsi Berbasis Android

To determine the topic or research title for the student's thesis, it is possible that the similarity of the title to the previous title, either accidentally or unintentionally. Therefore, the relevant section must check the titles submitted by students, so as to avoid plagiarism. The development of a thesis title similarity detection application is one of the efforts that can be made in solving this problem. The development of sosftware requires the right method to develop applications according to user needs. Extreme Programming (XP) is a part of the agile development approach based on iterative development, which is based on needs and solutions through collaboration among the development team. So, in this study, the development of a similarity detection application for the title of a thesis with the XP software development approach was carried out. Keywords - agile development, extreme programming, thesi

The relationship between sulfur metabolism and tolerance of hexavalent chromium in Scenedesmus acutus (Spheropleales): Role of ATP sulfurylase

Sulfur availability and the end products of its metabolism, cysteine, glutathione and phytochelatins, play an important role in heavy metal tolerance, chromium included. Sulfate and chromate not only compete for the transporters but also for assimilation enzymes and chromium tolerance in various organisms has been associated to differences in this pathway. We investigated the mechanisms of Cr(VI)-tolerance increase induced by S-starvation focusing on the role of ATP sulfurylase (ATS) in two strains of Scenedesmus acutus with different chromium sensitivity. S-starvation enhances the defence potential by increasing sulfate uptake/assimilation and decreasing chromium uptake, thus suggesting a change in the transport system. We isolated two isoforms of the enzyme, SaATS1 and SaATS2, with different sensitivity to sulfur availability, and analysed them in S-sufficient and S-replete condition both in standard and in chromium supplemented medium. SaATS2 expression is different in the two strains and presumably marks a different sulfur perception/exploitation in the Cr-tolerant. Its induction and silencing are compatible with a role in the transient tolerance increase induced by S-starvation. This enzyme can however hardly be responsible for the large cysteine production of the Cr-tolerant strain after starvation, suggesting that cytosolic rather than chloroplastic cysteine production is differently regulated in the two strains

The use of a genetic strategy to study the role of modulation of oxidative stress by uncoupling proteins 2 and 3 in the pathogenesis of Type 2 Diabetes

Mitochondrial dysfunction has been implicated in the early pathogenesis of Type 2 Diabetes. The uncoupling proteins 2 and 3 are mitochondrial proteins found in man that have been implicated in protecting mammals from the effects of over-nutrition. Examination of the effect of genetic variation in the UCP2-UCP3 genetic cluster has so far been inconclusive. The aim of this thesis was to examine, using a genetic strategy, the hypothesis that the role of the uncoupling proteins 2 and 3 in the pathogenesis of Type 2 Diabetes is via modification of oxidative stress. In a prospective study of nearly 3000 men the risk of type 2 diabetes at 10 years was increased for both the UCP2-866AA (1.94 [1.18-3.19]: p=0.009) and the UCP3-55TT (2.06 [1.06-3.99]: p=0.03) homozygotes. This increased risk was not explained by the association with any measured conventional risk factors. Paradoxically, in a Europe-wide cross-sectional study of 598 subjects the UCP2-866A variant was associated with lower waist-hip ratio (GX v AA,1.00 [0.06] v 0.98 [0.07]; p=0.003), although also associated with lower insulin secretion (42.6 [24.6] v 35.6 [18.6]; p=0.03). The UCP3 variant was not significantly associated with any metabolic trait. The significant heritability of plasma markers of oxidative stress (TAS 0.54, TOAS 0.49) suggests anti-oxidant function is a plausible mechanism to determine Type 2 Diabetes risk. The predictors of anti-oxidant stress in a family study were examined, as was the impact of UCP2-UCP3 gene cluster variation. Genetic variation in the UCP2-UCP3 was found to increase the risk of the Type 2 diabetes. While UCP2 may modify insulin secretion directly, the mechanism of action for UCP3 is likely to involve novel risk factors for Type 2 Diabetes such as modification of mitochondrial oxidative stress. Finally, the development of a human model is described to examine genetic influences on oxidative stress burden using a meal rich in used cooking oil.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Tetrodotoxins (Ttxs) and vibrio alginolyticus in mussels from central adriatic sea (italy): Are they closely related?

Tetrodotoxins (TTXs), potent neurotoxins, have become an increasing concern in Europe in recent decades, especially because of their presence in mollusks. The European Food Safety Authority published a Scientific Opinion setting a recommended threshold for TTX in mollusks of 44 µg equivalent kg−1 and calling all member states to contribute to an effort to gather data in order to produce a more exhaustive risk assessment. The objective of this work was to assess TTX levels in wild and farmed mussels (Mytilus galloprovincialis) harvested in 2018–2019 along the coastal area of the Marche region in the Central Adriatic Sea (Italy). The presence of Vibrio spp. carrying the non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes, which are suspected to be involved in TTX biosynthesis, was also investigated. Out of 158 mussel samples analyzed by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILICMS/MS), 11 (7%) contained the toxins at detectable levels (8–26 µg kg−1 ) and 3 (2%) contained levels above the EFSA safety threshold (61–76 µg kg−1 ). Contaminated mussels were all harvested from natural beds in spring or summer. Of the 2019 samples, 70% of them contained V. alginolyticus strains with the NRPS and/or PKS genes. None of the strains containing NRPS and/or PKS genes showed detectable levels of TTXs. TTXs in mussels are not yet a threat in the Marche region nor in Europe, but further investigations are surely needed

The sign of psoriasis in mesenchymal stem cells of the skin

Psoriasis is a chronic inflammatory and immune-mediated skin disease, characterized by epidermal hyperproliferation, abnormal keratinocyte differentiation and angiogenesis, whose skin lesions are promoted by exogenous and endogenous factors. The cutaneous and systemic over-expression of several pro-inflammatory cytokines, observed in the initiation, maintenance and recurrence of skin lesions, is known to be caused also by reactive oxygen species (ROS). For this reason it has been postulated that ROS production and compromised function of antioxidant system may be involved in the pathogenesis of psoriasis. Some typical features of psoriasis, like growth rate, expression of VEGF and iNOS as well as the production of VEGF and nitric oxide (NO) and some antioxidant responses, have already been extensively evaluated in differentiated cells of psoriatic skin, but no indications are still available about, the mesenchymal stem, that may be isolated from skin (S-MSCs)

Application of a Weight of Evidence Approach for Monitoring Complex Environmental Scenarios: the Case-Study of Off-Shore Platforms

Multidisciplinary investigations based on integration of chemical and biological measurements, represent an added value to monitoring and management protocols, and their use is recommended by European Directives to evaluate the environmental status of aquatic ecosystems. However, assessing the overall significance of results obtained in different typologies of studies is often a difficult challenge. The aim of this work was to present a quantitative Weight Of Evidence (WOE) model (Sediqualsoft) to integrate huge amounts of heterogeneous data and to validate this approach in complex monitoring scenarios. Using the case-study of an off-shore platform field in the Adriatic Sea, procedures are presented to elaborate different typologies of data (lines of evidence, LOEs), including chemical characterization of sediments, bioavailability, biomarkers, ecotoxicological bioassays and benthic communities around three platforms. These data are initially evaluated by logical flowcharts and mathematical algorithms, which provide specific hazard indices for each considered LOE, before their different weighting and overall integration in an environmental risk index. The monitoring study selected for the WOE elaboration consisted on chemical analyses of trace metals, aliphatic hydrocarbons, polycyclic aromatic hydrocarbons carried out on 60 sediment samples; the same samples were also characterized for the status of benthic communities; bioavailability of metals from sediments was assessed in laboratory conditions on the polychaete Hediste diversicolor, while bioaccumulation of inorganic and organic chemicals and biomarker responses were measured in native and transplanted mussels; ecotoxicological properties of sediments were evaluated through a battery of bioassays determining algal growth of the diatom Phaeodactylum tricornutum, bioluminescence of the marine bacterium Vibrio fischeri, survival of the copepod Acartia tonsa and embryotoxicity of sea urchin Paracentrotus lividus. Overall, almost 7000 analytical results were elaborated and summarized in specific hazard indices. The WOE integration of multiple typologies of data allowed more robust and weighted conclusions compared to the use of individual LOEs, highlighting the feasibility of this procedure for multidisciplinary monitoring and risk assessment approaches. On a practical side, the WOE evidences also suggested a revision of actual monitoring procedures. Overall, the proposed WOE model appeared as a useful tool to summarize large datasets of complex data in integrative indices, and to simplify the interpretation for stakeholders and decision makers, thus supporting a more comprehensive process of "site-oriented" management decisions

Short-and long-term exposure to heavy metals induced oxidative stress response in Pseudokirchneriella subcapitata

Algal cells can be exposed to toxicants for a short term due to accidental discharges or, more commonly, for a long term. The present work aimed to assess the ability of Cd, Cr, Cu and Zn to induce accumulation of reactive oxygen species (ROS) in the alga Pseudokirchneriella subcapitata after a short (6?h) or a long (72?h) exposure time. The relationship between the ROS induction and the content of reduced glutathione (GSH) was also examined. For this purpose, three nominal concentrations of each metal were used corresponding approximately to 72 h-EC10 and 72 h-EC50 values and a high concentration (>72 h-EC90 values). Intracellular ROS accumulation and GSH content were evaluated using a fluorescent-based approach. A long-term (chronic) exposure of algal cells to Cd, Cu and Zn, at the highest concentrations tested, induced an increase of intracellular ROS and GSH content. The increase of GSH content might be a form of algal cells to redress the imbalance caused by the oxidative stress. However, the increase of GSH was not enough to protect the algal cells against the long-term exposure to oxidative stress. The exposure of algal cells to low or intermediate metals concentrations induced a modification of GSH content; however, no increase of ROS production was detected, which indicates that the toxic symptoms exhibited by algal cells, under these conditions, cannot be attributed to intracellular ROS accumulation.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic founding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER- 006684), and the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124- FEDER-027462). Manuela D. Machado gratefully acknowledges the post-doctoral grant from FCT (SFRH/BPD/72816/2010)