Síndrome de Gorlin

[ES]El síndrome de Gorlin es una enfermedad infrecuente de herencia autosómica dominante producida por mutaciones en genes de la vía de señalización Sonic Hedgehog, entre los que destaca PTCH1. Se caracteriza por el desarrollo de múltiples carcinomas basocelulares en edades tempranas, que pueden ir asociados a otras manifestaciones cutáneas como pits palmoplantares, o a manifestaciones extracutáneas, entre las que destacan los queratoquistes odontogénicos y el meduloblastoma. El papel del dermatólogo es importante en la sospecha de este síndrome, pero suele ser necesario un equipo multidisciplinar en el diagnóstico, seguimiento y en el tratamiento de estos pacientes. El tratamiento dermatológico puede ser complicado debido al alto número de carcinomas basocelulares y a su extensión. En los últimos años se han desarrollado nuevos fármacos que inhiben la vía Sonic Hedgehog y parecen prometedores para estos pacientes, aunque su eficacia está limitada por los efectos secundarios y la creación de resistencias.[EN]Gorlin syndrome is a rare autosomal dominant disease caused by mutations in the sonic hedgehog signaling pathway. Of particular importance is the PTCH1 gene. The disease is characterized by the development of multiple basal cell carcinomas at young ages. These tumors may present with other skin manifestations such as palmoplantar pits and with extracutaneous manifestations such as odontogenic keratocysts and medulloblastoma. Although the dermatologist may be key for recognizing clinical suspicion of the syndrome, a multidisciplinary team is usually necessary for diagnosis, treatment, and follow-up. Skin treatment may be complicated due to the large number of basal cell carcinomas and the extent of involvement

RESISTENCIA AL RECEMENTADO DE BRACKETS METÁLICOS UTILIZANDO DIFERENTES SISTEMAS ADHESIVOS Y ACONDICIONAMIENTO. ESTUDIO IN-VITRO

Material científico de alta importancia para la práctica clínica especializada en Ortodoncia, puesto que brinda información relevante para la recementación de anclajes ortodóncicos.Objetivo: Evaluar la resistencia al recementado de brackets metálicos descementados utilizando adhesivos autograbables y adhesivos convencionales. Material y Métodos: Estudio experimental, transversal, comparativo, diseño in-vitro. Realizado en el C.I.E.A.O. de la UAEMex. Muestra: 90 premolares íntegros extraídos por razones ortodóncicas, sin caries, fracturas o lesiones, divididos aleatoriamente en tres grupos(n=30). Grupo I: se utilizó adhesivo autograbante y brackets metálicos nuevos; Grupo II: brackets descementados arenados/ácido fosfórico 37%; Grupo III: brackets descementados arenados/ácido fosfórico 37%/adhesivo en la malla del bracket y diente. Se utilizó una máquina de ensayos universales para medir la resistencia al recementado, la prueba estadística utilizada fue: ANOVA-un factor p < 0.05, la carga se midió en MPa y se registró la cantidad de adhesivo remanente (ARI). Resultados: La resistencia al descementado mostró que el valor medio del grupo III (19.4 ± 4.7 MPa) presentó diferencias significativas en comparación con los grupos I (p= 0.0001, 9.5 ± 4.3 MPa) y II (p= 0.0001, 14.4 ± 4.7 MPa). Asimismo elgrupo II (p=0.0001, 14.4 ± 4.7 MPa) presentó diferencias significativas con el grupo III (p= 0.0001, 19.4 ± 4.74 MPa). Los resultados del ARI mediante la prueba X2 (21.61) revelaron que los grupos son significativamente diferentes (p=0.001). Conclusiones: Los brackets metálicos recementados pueden ser adheridos exitosamente con cualquiera de estos sistemas. El prerrevestimiento con adhesivo sobre la malla del bracket previamente arenado, incrementa la resistencia al descementado

Differences in E-Cadherin and Syndecan-1 Expression in Different Types of Ameloblastomas

Ameloblastomas are a group of benign, locally aggressive, recurrent tumors characterized by their slow and infiltrative growth. ECadherin and syndecan-1 are cell adhesion molecules related to the behavior of various tumors, including ameloblastomas. Ninetynine ameloblastoma samples were studied; the expression of E-cadherin and syndecan-1 were evaluated by immunohistochemistry. E-Cadherin and epithelial syndecan-1 were more highly expressed in intraluminal/luminal unicystic ameloblastom a than in mural unicystic ameloblastoma and solid/multicystic ameloblastoma, whereas the stromal expression of syndecan-1 was higher in mural unicystic ameloblastoma and solid/multicystic ameloblastoma. Synchronicity was observed between E-cadherin and epithelial syndecan-1; the expression was correlated with intensity in all cases. There was a strong association between expression and tumor size and recurrence. The evaluation of the expression of E-cadherin and syndecan-1 are important for determining the potential aggressiveness of ameloblastoma variants. Future studies are required to understand how the expression of these markers is related to tumor aggressiveness

Actions for the Restoration of the Biodiversity of Forest Ecosystems in Cuba

The Earth is threatened daily by the degradation of its ecosystems due to fragmentation. One of the main consequences is biodiversity loss. Despite the economic progress and conservation actions carried out in many countries, the planet is losing genuine tropical forest due to diverse reasons. This chapter presents the results obtained during the restoration of three tropical forests in Cuba: 1) the mesophyll semi-deciduous forest in the western sector of the Biosphere Reserve 'Sierra del Rosario' (BRSR); 2) the riverside forest of the Cuyaguateje River in western Cuba; and 3) the exploited native rainforests of the sector Quibiján-Naranjal of the River Toa in eastern Cuba

Surgical treatment for liver haematoma following endoscopic retrograde cholangiopancreatography: An unusual case

AbstractBackgroundEven in expert hands, there can be serious complications when performing an endoscopic retrograde cholangiopancreatography. The most frequent complications are pancreatitis, cholangitis, bleeding, perforation, and acute cholecystitis. The hepatic subcapsular haematoma is a rare complication, with few cases described worldwide.ObjectiveA case is presented of an extremely rare complication of endoscopic retrograde cholangiopancreatography, which required surgical treatment for its resolution without success. This is second case of mortality reported in the literature.Clinical caseFemale patient of 30 years old, with indication for endoscopic retrograde cholangiopancreatography due to benign strictures. A hydro-pneumatic dilation and stent placement of 2 gauge 10Fr was performed. She presented abdominal pain after the procedure and significant decline in haemoglobin with no evidence of haemodynamic instability so an abdominal tomography scan was performed, showing no evidence of liver injury. The patient was haemodynamic unstable within 72h. A laparotomy was required for damage control, with fatal outcome in the intensive care unit due to multiple organ failure.ConclusionSubcapsular hepatic haematoma after endoscopic retrograde cholangiopancreatography is a rare complication, with few cases reported in the literature. Treatment described in the literature is conservative, resulting in a satisfactory resolution

Current concepts in ameloblastoma-targeted therapies in B-raf proto-oncogene serine/threonine kinase V600E mutation: Systematic review

Ameloblastomas are common benign epithelial odontogenic neoplasms that present an aggressive and unpredictable behavior that may modify treatment strategies. Different signaling pathways that participate in the progression of these tumors have been identified. B-raf proto-oncogene serine/threonine kinase (BRAF) is a protein involved in the behavior of ameloblastomas, and it is related to many cell mechanisms. BRAF gene mutations have been identified in ameloblastomas, of which the BRAF V600E (valine substituted by glutamic acid at amino acid 600) mutation has been the most common and can be present concomitantly with other mutations that may be involved in its behavior. Targeted therapies have been used as an alternative in the case of resistance or contraindications to conventional treatments

Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas

Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of these methods. The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results. Therefore, this automated cell counting method must be supplemented with manual cell counting methods

Molecular Markers of Anticancer Drug Resistance in Head and Neck Squamous Cell Carcinoma: A Literature Review

This manuscript provides an update to the literature on molecules with roles in tumor resistance therapy in head and neck squamous cell carcinoma (HNSCC). Although significant improvements have been made in the treatment for head and neck squamous cell carcinoma, physicians face yet another challenge—that of preserving oral functions, which involves the use of multidisciplinary therapies, such as multiple chemotherapies (CT) and radiotherapy (RT). Designing personalized therapeutic options requires the study of genes involved in drug resistance. This review provides an overview of the molecules that have been linked to resistance to chemotherapy in HNSCC, including the family of ATP-binding cassette transporters (ABCs), nucleotide excision repair/base excision repair (NER/BER) enzymatic complexes (which act on nonspecific DNA lesions generated by gamma and ultraviolet radiation by cross-linking and forming intra/interchain chemical adducts), cisplatin (a chemotherapeutic agent that causes DNA damage and induces apoptosis, which is a paradox because its effectiveness is based on the integrity of the genes involved in apoptotic signaling pathways), and cetuximab, including a discussion of the genes involved in the cell cycle and the proliferation of possible markers that confer resistance to cetuximab

Immune system disorders, cancer and viral infections: A new treatment opportunity for the immune checkpoint inhibitors

Producción CientíficaThe relationship between viral infections and cancer is well known and has been established for decades. Multiple tumours are generated from alterations secondary to viral infections 2 resulting from a dysregulation of the immune system in many cases. Certain causal relationships, such as that between the Epstein–Barr virus (EBV) in nasopharyngeal cancer or hepatitis C and B viruses in hepatocarcinoma, have been clearly established, and their implications for the prognosis and treatment of solid tumours are currently unknown. Multiple studies have evaluated the role that these infections may have in the treatment of solid tumours using immunotherapy. A possible relationship between viral infections and an increased response to immune checkpoint inhibitors (ICIs) has been established at a theoretical level in solid neoplasms, such as EBV-positive cavum cancer and human papillomavirus (HPV)-positive and oropharyngeal cancer. These could yield a greater response associated with the activation of the immune system secondary to viral infection, the consequence of which is an increase in survival in these patients. That is why the objective of this review is to assess the different studies or clinical trials carried out in patients with solid tumours secondary to viral infections and their relationship to the response to ICIs