Community-linked maternal death review (CLMDR) to measure and prevent maternal mortality: a pilot study in rural Malawi.

In Malawi, maternal mortality remains high. Existing maternal death reviews fail to adequately review most deaths, or capture those that occur outside the health system. We assessed the value of community involvement to improve capture and response to community maternal deaths

Seed germination and in vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis

Propagation of the medicinal and pesticidal tree, Bobgunnia madagascarensis is difficult due to poor and erratic germination of its seeds and slow growth of its seedlings. This study involved two separate experiments. The first evaluated the effect of pre-sowing treatments and growing medium on ex vitro seed germination and early seedling development. The second experiment involved in vitro germination, shoot initiation and rooting of shoots. Pre-sowing seed treatments involved soaking seeds in cold and hot water for 12 and 24 h and soaking in different concentrations (0, 100, 200, 400 and 800 mg/l) of gibberellic acid for 24 h. Soaking of seeds in cold or hot water for up to 24 h did not achieve more than 45% germination, while seeds treated with gibberellic acid achieved <20% germination rates. On the other hand, in vitro procedures achieved 30 to 70% germination of seeds. Seedling survival of ex-vitro germinated seeds was higher (>76%) when seeds were sown in a growing medium without compost compared with a medium with compost (<43%). All shoot-tips isolated from the in vitro germinated seedlings on B5 media without plant growth regulators continued to grow as a single shoot, while shoot-tips cultured on B5 supplemented with 0.1 mg/l of naphthaleneacetic acid (NAA) and thidiazuron (TDZ) produced two shoots each after four weeks. It was concluded that B. madagascariensis seeds had very low ex vitro germination percentages. Although, in vitro cultures improved seed germination, axillary shoot multiplication and rooting were not satisfactory. Therefore, further studies are needed to develop an optimal in vitro multiplication protocol for B. madagascariensis.Key words: Axillary shoot multiplication, gibberellic acid, in vitro regeneration, seed germination, Swartzia

Cowpea (Vigna unguiculata (L.) Walp) for food security: an evaluation of end-user traits of improved varieties in Swaziland

Open Access JournalImproved varieties have agronomic advantages over local varieties,but not much attention has been given to understand the nutritional content of the improved cowpea varieties released in Swaziland. This study investigated the physical and nutritional properties of improved cowpea varieties released in Swaziland. Five improved varieties (IT-04K-321-2, IT-97K-390-2, IT-18, IT-16, and IT-99K-494-6) and one local variety (Mtilane, as check) were analyzed for physical and chemical properties. The results showed that there were variations in seed weight as the values ranged between 12.5 and 18.5 g per 100 g seed weight. The protein content ranged from 25.38% to 27.56% with no significant difference (P 0.05) among the varieties. The improved varieties have high seed weight, which is an essential factor that farmers consider when choosing a variety to adopt. In terms of addressing nutritional security, the crop is suitable for addressing protein-energy malnutrition and formulating blends for baby foods in Swaziland

Scaling up community-based goat breeding programmes via multi-stakeholder collaboration

Community-based livestock breeding programmes (CBBPs) have emerged as a potential approach to implement sustainable livestock breeding in smallholder systems. In Malawi and Uganda, goat CBBPs were introduced to improve production and productivity of indigenous goats through selective breeding. Scaling up CBBPs have recently received support due to evidence-based results from current implementation and results of CBBPs implemented in other regions of the world. This paper explores strategies for scaling up goat CBBPs in Malawi and Uganda, and documents experiences and lessons learned during implementation of the programme. A number of stakeholders supporting goat-based interventions for improving smallholders’ livelihoods exists. This offers an opportunity for different actors to work together by pooling financial resources and technical expertise for establishment and sustainability of goat CBBPs. Scaling up strategies should be an integral part of the pilot design hence dissemination partners need to be engaged during the design and inception stages of the pilot CBBPs. Creation of self-sustaining CBBPs requires early collaborative programme planning, meaningful investment and long-term concerted and coordinated efforts by collaborating partners. Permanently established actors, like government agencies and research and training institutions, are better placed to coordinate such efforts. The overall goal of the scaling up programme should be creation of a financially sustainable system, in which smallholders are able, on their own, to transact and sustain operations of their local breeding institutions using locally generated revenue/ resources. Since CBBP scaling up is a ‘learning by doing process’, an effective monitoring and evaluation system should be an integral part of the process

Lactobacillus-deficient vaginal microbiota dominate post-partum women in rural Malawi

The bacterial community found in the vagina is an important determinant of a woman's health and disease. A healthy vaginal microbiota is associated with a lower species richness and high proportions of one of a number of different Lactobacillus spp.. When disrupted the resulting abnormal vaginal microbiota is associated with a number of disease states and poor pregnancy outcomes. Studies up until now have concentrated on relatively small numbers of American and European populations which may not capture the full complexity of the community, nor adequately predict what constitutes a healthy microbiota in all populations. In this study we sampled and characterised the vaginal microbiota from a cohort of 1107 women in rural Malawi found on vaginal swabs taken post-partum. We found a population dominated by Gardnerella vaginalis and devoid of the most common vaginal Lactobacillus species, even if the vagina was sampled over a year post-partum. The Lactobacillus-deficient anaerobic community commonly labelled community state type (CST) 4 could be sub-divided into four further communities. A Lactobacillus iners dominated vaginal microbiota became more common the longer after delivery the vagina was sampled, but G. vaginalis remained the dominant organism. These results outline the difficulty in all-encompassing definitions of what a healthy or abnormal vaginal microbiota is post-partum. Previous identification of community state types and associations between bacterial species, bacterial vaginosis and adverse birth outcomes may not represent the complex heterogeneity of the microbiota present.ImportanceA bacterial community in the vaginal tract that is dominated by small number of bacterial Lactobacillus species and when they are not present, there is a greater incidence of inflammatory conditions and adverse birth outcomes. A switch to a vaginal bacterial community lacking in Lactobacillus species is common after pregnancy. In this study we characterised the vaginal microbiota after delivery of a large group of women from a resource poor, under-sampled population in rural Malawi. The majority of women were found to have a Lactobacillus-deficient community and even after a year after delivery the majority of women still did not have Lactobacillus present in their vaginal microbiota. The effect of becoming pregnant again for those who do not revert to a Lactobacillus dominant community is unknown and could suggest that not all Lactobacillus-deficient community structures are adverse. A better understanding is needed of this complex community state type

Assessing the post-harvest constraints in smallholders’ groundnut production: A Survey in central Malawi

An in-depth scoping survey was conducted on 248 smallholder farmers producing groundnut in Malawi to delineate the constraints in production, especially on-farm post-harvest operations, while revealing various aspects in the status of production and consumption practices. The insightful outcomes included the farmers’ perception of the post-harvest operations as highly labor demanding, being the major obstacle to production expansion for the lucrative and nutritious crop. In particular, shelling, lifting, and stripping were the top three processes of remarkable labor intensity. The respondents expressed the intention for scale-up as long as the labor constraints were mitigated, with expected welfare gain through increased income, improved nutrition, and reduced aflatoxin contamination, as well as mitigated drudgery for women

Physicochemical, microbial, and aflatoxin analyses of selected high-quality cassava flour (HQCF) from the major markets of Zambia

Open Access Journal; Published online: 13 May 2021Various quality grades of high-quality cassava flour (HQCF) are found in markets due to the rapid increase in its utilization for product development. Therefore, this study assessed the chemical, functional and pasting properties and the microbial and aflatoxin content of HQCF being sold in Lusaka markets. Nine samples of HQCF (coded CM 01 to 09) were collected from supermarkets, one major open market, and one cassava processing centre. The samples were analyzed for chemical, functional and pasting properties and the microbial and aflatoxin content using standard laboratory methods. Samples CM 01, CM 08, and CM 09 had starch content above 60%, while CM 02 to CM 07 had starch slightly above 40%. The mean value of bulk density (BD) was 0.51 ± 25.49 g ml−1, dispersibility 68 ± 2.63%, swelling power (SP) 7.84 ± 0.76%, solubility index 135.80 ± 18.8%, and water absorption capacity (WAC) 6.28 ± 32.49%. The mean value for the peak viscosity was 437.46 ± 94.12 RVU, trough viscosity 217.55 ± 27.41 RVU, breakdown viscosity 219.91 ± 77.94 RVU, final viscosity 284.31 ± 29.96 RVU, and setback viscosity 66.77 ± 5.30 RVU. There was no detection of aflatoxins B1, B2, G1, and G2, especially aflatoxin B1 (AFB1) that belongs to group 1 carcinogens for humans. Bacteria colony counts in samples CM 02, CM 03, CM 07, and CM 09 were higher with CFU mg−1 of 2,280,000, 260,000, 200,000, and 510,000, respectively. The study’s information will guide the quality standard specifications, breeding programs, and end-use of HQCF

Urine selenium concentration is a useful biomarker for assessing population level selenium status

Plasma selenium (Se) concentration is an established population level biomarker of Se status, especially in Se-deficient populations. Previously observed correlations between dietary Se intake and urinary Se excretion suggest that urine Se concentration is also a potentially viable biomarker of Se status. However, there are only limited data on urine Se concentration among Se-deficient populations. Here, we test if urine is a viable biomarker for assessing Se status among a large sample of women and children in Malawi, most of whom are likely to be Se-deficient based on plasma Se status. Casual (spot) urine samples (n = 1406) were collected from a nationally representative sample of women of reproductive age (WRA, n =741) and school aged children (SAC, n=665) across Malawi as part of the 2015/16 Demographic and Health Survey. Selenium concentration in urine was determined using inductively coupled plasma mass spectrometry (ICP-MS). Urinary dilution corrections for specific gravity, osmolality, and creatinine were applied to adjust for hydration status. Plasma Se status had been measured for the same survey participants. There was between-cluster variation in urine Se concentration that corresponded with variation in plasma Se concentration, but not between households within a cluster, or between individuals within a household. Corrected urine Se concentrations explained more of the between-cluster variation in plasma Se concentration than uncorrected data. These results provide new evidence that urine may be used in the surveillance of Se status at the population level in some groups. This could be a cost-effective option if urine samples are already being collected for other assessments, such as for iodine status analysis as in the Malawi and other national Demographic and Health Surveys

New organizational forms in emerging economies: bridging the gap between agribusiness management and international development

This is the author accepted manuscript. The final version is available from Emerald via the DOI in this recordPurpose: This editorial article introduces and analyzes a variety of new organizational forms that rapidly emerged in Africa, Asia, Latin America and Eastern Europe in the latest two decades. Among the others, these include: business model partnerships, business platforms, incubators and hubs, public–private partnerships, agribusiness companies' foundations and spin-offs, short supply chains, community-supported agriculture and other community self-organizing experiences. Building upon the recent literature and the five selected papers in this special issue, the authors discuss what is novel in these organizations and why, when and how they emerge and evolve over time. Design/methodology/approach: The authors identify three elements that, when considered together, explain and predict the emergence and evolution of these new organizational forms: institutions, strategies and learning processes. Findings: The authors demonstrate that societal actors seeking to (re)design these new organizational forms need to consider these three elements to combine the pursuit of their interests of their own constituencies with the sustainable development goals (SDGs). Originality/value: Taking stock from the literature, the authors invite future research on new organizational forms to take explicitly the pursuit of the SDGs into consideration; to build upon a process ontology; and to deeply reflect on our positionality of scientists studying and sometimes engaging in these organizations