Comparing Pfizer GeneSTAR and Igenity PROFILE DNA tests in Crossbred Cattle

Our objective was to compare the performance of Pfizer’s GeneSTAR and Igenity’s PROFILE DNA tests in crossbred beef cattle. Hair follicles were collected from 394 crossbred steers that were part of the South Dakota State University Calf Value Discovery project (n = 145) and calves that were fed at the SDSU Southeast Research Farm (n = 249) in 2011. Hair follicles were sent to Pfizer Animal Genetics and Igenity for testing with their GeneSTAR and PROFILE DNA tests, respectively. Marbling score (100-999 scale), ribeye area, fat thickness, carcass weight, yield grade, quality grade, and % kidney, pelvic, and heart fat was collected post-harvest on all steers. Using this dataset, we first asked whether each DNA test was associated with marbling scores. If the DNA tests were not associated with marbling, then the tests may not be useful for predicting genetic merit for marbling in crossbred cattle. The GeneSTAR and PROFILE DNA tests were associated with marbling scores, but this association was not high. Second, we asked whether GeneSTAR and PROFILE DNA test results were associated with each other. If test results were not correlated, then using both DNA test results together may improve genetic predictions. GeneSTAR and PROFILE tests for tenderness were highly correlated, suggesting that DNA markers for tenderness are similar for both tests. GeneSTAR and PROFILE DNA tests for marbling and feed efficiency were not highly correlated, raising the possibility that both tests could be used jointly to improve genetic predictions for these traits. However, using both GeneSTAR and PROFILE DNA tests jointly did not appreciably improve predictions for marbling score in our dataset. We conclude that, although both GeneSTAR and PROFILE DNA tests were associated with marbling score, the correlation between these tests and marbling was low in our sample of crossbred cattle. Further, using GeneSTAR and PROFILE DNA tests for marbling and feed efficiency may improve genetic predictions for these traits, but we did not find evidence that using both tests jointly improves predictions for marbling

Differential gene expression in the Longissimus dorsi of Nguni and Bonsmara bulls finished on low and high energy diets

Objectives of this research were to examine differential gene expression profiles of Nguni and Bonsmara cattle fed diets differing in their energy density. The ultimate goal was to improve understanding of the mechanisms that underlie differences between these breeds and the potential interactions of the differences between breeds with the nutritive environment. The experiment was designed as a 2 × 2 factorial arrangement of breed and diet (12.5 MJ/kg DM vs. 10.9 MJ/kg DM). The initial feeding trial had 10 bull calves per treatment. However, financial constraints limited RNA sequencing to six animals per treatment and the RNA generated from one animal was of insufficient quality to be useful. Transcripts with false discovery rate P-values 2.0 were considered significant. Bonsmara had a faster growth rate, heavier live and carcass weights, and better feed conversion compared to Nguni. However, lower levels of fat were observed in Nguni. Twenty different genes were differentially expressed, with three exhibiting interaction effects and all 20 having differences in transcript abundance between the breeds. A dietary effect was only observed for the one gene and that gene was also subject to an interaction effect with breed. Observed differences in gene expression between Bonsmara and Nguni by several genes affecting the structure or function of the mitochondria imply differences in energy metabolism between the breeds. Interaction effects on the abundance of some gene transcripts indicate the need to consider the diet when evaluating breed differences and conversely, consider breed when evaluating diets.Red Meat Research and Development South Africa and the National Research Foundation.http://www.sasas.co.zaam2024Animal and Wildlife SciencesSDG-02:Zero Hunge

Bringing online adaptive radiotherapy to a standard C-arm linac

Current online adaptive radiotherapy (oART) workflows require dedicated equipment. Our aim was to develop and implement an oART workflow for a C-arm linac which can be performed using standard clinically available tools. A workflow was successfully developed and implemented. Three patients receiving palliative radiotherapy for bladder cancer were treated, with 33 of 35 total fractions being delivered with the cone-beam computed tomography (CBCT)-guided oART workflow. Average oART fraction duration was 24 min from start of CBCT acquisition to end of beam on. This work shows how oART could be performed without dedicated equipment, broadening oART availability for application at existing treatment machines

Bringing online adaptive radiotherapy to a standard C-arm linac

Current online adaptive radiotherapy (oART) workflows require dedicated equipment. Our aim was to develop and implement an oART workflow for a C-arm linac which can be performed using standard clinically available tools. A workflow was successfully developed and implemented. Three patients receiving palliative radiotherapy for bladder cancer were treated, with 33 of 35 total fractions being delivered with the cone-beam computed tomography (CBCT)-guided oART workflow. Average oART fraction duration was 24 min from start of CBCT acquisition to end of beam on. This work shows how oART could be performed without dedicated equipment, broadening oART availability for application at existing treatment machines