Perceptions of Self-Efficacy & Support Among Secondary Early-Career Teachers and their Principals During the COVID-19 Pandemic

In response to challenges faced by middle and high school educators during the COVID-19 pandemic, a study was conducted in the Spring of 2021 involving 33 early-career mathematics teachers and eight supervising school principals in the State of California. These participants completed detailed surveys which provided demographic information, as well as perceptions of support, efficacy and job satisfaction. Findings show a variety of associations among teacher perceptions of support and their efficacy and job satisfaction in the face of challenging circumstances. As it related to principal support and recognition, principal participants expressed confidence in their ability to support teachers as a result of the pandemic, including understanding best practices for evaluating teachers for online teaching. Additionally, while agreeing that teachers at their schools needed more professional development in instructional practice, participating school principals felt they supported their teachers as they adapted instruction due to the COVID-19 pandemic. This study aspires to inform practitioners and policymakers alike in their understanding of teachers and their principals as they responded to significant challenges as a result of the COVID-19 pandemic

Independent large scale duplications in multiple M. tuberculosis lineages overlapping the same genomic region

Mycobacterium tuberculosis, the causative agent of most human tuberculosis, infects one third of the world's population and kills an estimated 1.7 million people a year. With the world-wide emergence of drug resistance, and the finding of more functional genetic diversity than previously expected, there is a renewed interest in understanding the forces driving genome evolution of this important pathogen. Genetic diversity in M. tuberculosis is dominated by single nucleotide polymorphisms and small scale gene deletion, with little or no evidence for large scale genome rearrangements seen in other bacteria. Recently, a single report described a large scale genome duplication that was suggested to be specific to the Beijing lineage. We report here multiple independent large-scale duplications of the same genomic region of M. tuberculosis detected through whole-genome sequencing. The duplications occur in strains belonging to both M. tuberculosis lineage 2 and 4, and are thus not limited to Beijing strains. The duplications occur in both drug-resistant and drug susceptible strains. The duplicated regions also have substantially different boundaries in different strains, indicating different originating duplication events. We further identify a smaller segmental duplication of a different genomic region of a lab strain of H37Rv. The presence of multiple independent duplications of the same genomic region suggests either instability in this region, a selective advantage conferred by the duplication, or both. The identified duplications suggest that large-scale gene duplication may be more common in M. tuberculosis than previously considere

Crystalline Silicate Emission in the Protostellar Binary Serpens--SVS20

We present spatially resolved mid-infrared spectroscopy of the class I/flat-spectrum protostellar binary system SVS20 in the Serpens cloud core. The spectra were obtained with the mid-infrared instrument T-ReCS on Gemini-South. SVS20-South, the more luminous of the two sources, exhibits a mid-infrared emission spectrum peaking near 11.3 \micron, while SVS20-North exhibits a shallow amorphous silicate absorption spectrum with a peak optical depth of $\tau \sim 0.3$. After removal of the the line-of-sight extinction by the molecular common envelope, the ``protostar-only'' spectra are found to be dominated by strong amorphous olivine emission peaking near 10 \micron. We also find evidence for emission from crystalline forsterite and enstatite associated with both SVS20-S and SVS20-N. The presence of crystalline silicate in such a young binary system indicates that the grain processing found in more evolved HAeBe and T Tauri pre-main sequence stars likely begins at a relatively young evolutionary stage, while mass accretion is still ongoing.Comment: Accepted for publication by The Astrophysical Journa

Enhanced hydrogenation catalyst synthesized by Desulfovibrio desulfuricans exposed to a radio frequency magnetic field

EPSRC (EP/I007806/1; EP/D05768X/1), BBSRC (BB/ C516128/1), NERC (NE/L014076/1), The Royal Society (Industrial Fellowship) and Spanish Government Sistema Nacional de Garantia Juvenil grant PEJ-2014-P-00391.This work was supported by EPSRC (grants No EP/ I007806/1 and EP/D05768X/1), BBSRC (grant No BB/ C516128/1), NERC (grant NE/L014076/1) and by a Royal Society Industrial Fellowship to LEM for secondment into C-Tech Innovation Ltd., who provided the bespoke apparatus used in this work. We acknowledge the invaluable contributions of the late Dr Ruth Wroe of C-Tech Innovation Ltd. into useful discussions and the kind permission of Drs S. Megit, C. Berry and A. Morby (University of Cardiff, UK) to show their unpublished work in Supplementary Information. This work was partially supported by the Spanish Government Sistema Nacional de Garantia Juvenil Grant PEJ-2014-P- 00391 (Promocion de Empleo Joven e Implantacion de la Garantia Juvenil 2014, MINECO) with a scholarship to JGB. We also thank the EM Centre at U. Granada for access to high-resolution electron microscopy (in Fig. S2 and S3). All authors declare no competing interests.Desulfovibrio desulfuricans reduces Pd(II) to Pd(0)-nanoparticles (Pd-NPs) which are catalytically active in 2-pentyne hydrogenation. To make Pd-NPs, resting cells are challenged with Pd(II) ions (uptake), followed by addition of electron donor to promote bioreduction of cell-bound Pd(II) to Pd(0) (bio-Pd). Application of radiofrequency (RF) radiation to prepared 5 wt% bio-Pd catalyst (60 W power, 60 min) increased the hydrogenation rate by 70% with no adverse impact on selectivity to cis-2-pentene. Such treatment of a 5 wt% Pd/carbon commercial catalyst did not affect the conversion rate but reduced the selectivity. Lower-dose RF radiation (2-8 W power, 20 min) was applied to the bacteria at various stages before and during synthesis of the bio-scaffolded Pd-NPs. The reaction rate (mu mol 2-pentyne converted s(-1)) was increased by similar to threefold by treatment during bacterial catalyst synthesis. Application of RF radiation (2 or 4 W power) to resting cells prior to Pd(II) exposure affected the catalyst made subsequently, increasing the reaction rate by 50% as compared to untreated cells, while nearly doubling selectivity for cis 2-pentene. The results are discussed with respect to published and related work which shows altered dispersion of the Pd-NPs made following or during RF exposure.UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) EP/I007806/1 EP/D05768X/1UK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC) BB/C516128/1UK Research & Innovation (UKRI)Natural Environment Research Council (NERC) NE/L014076/1Royal Society of London European CommissionSpanish Government Sistema Nacional de Garantia Juvenil grant PEJ-2014-P-0039

Endogenous retrovirus RNA expression differences between race, stage and HPV status offer improved prognostication among women with cervical cancer

Endogenous human retroviruses (ERVs) are remnants of exogenous retroviruses that have integrated into the human genome. Using publicly available RNA-seq data from 63 cervical cancer patients, we investigated the expression of ERVs in cervical cancers. Four aspects of cervical cancer were investigated: patient ancestral background, tumor HPV type, tumor stage and patient survival. Between the racial subgroups, 74 ERVs were significantly differentially expressed, with Black Americans having 30 upregulated and 44 downregulated (including MER21C, HERV9-int, and HERVH-int) ERVs when compared to White Americans. We found that 3313 ERVs were differentially expressed between HPV subgroups, including MER41A, HERVH-int and HERVK9. There were 28 downregulated (including MLT1D and HERVH-int) and 61 upregulated (including MER41A) ERVs in locally advanced-stage compared to early-stage samples. Tissue microarrays of cervical cancer patients were used to investigate the protein expression of ERVs with protein coding potential (i.e., HERVK and ERV3). Significant differences in protein expression of ERV3

Thermal Liability of Hyaloclastite in the Krafla Geothermal Reservoir, Iceland:The Impact of Phyllosilicates on Permeability and Rock Strength

Geothermal fields are prone to temperature fluctuations from natural hydrothermal activity, anthropogenic drilling practices, and magmatic intrusions. These fluctuations may elicit a response from the rocks in terms of their mineralogical, physical (i.e., porosity and permeability), and mechanical properties. Hyaloclastites are a highly variable volcaniclastic rock predominantly formed of glass clasts that are produced during nonexplosive quench-induced fragmentation, in both subaqueous and subglacial eruptive environments. They are common in high-latitude geothermal fields as both weak, highly permeable reservoir rocks and compacted impermeable cap rocks. Basaltic glass is altered through interactions with external water into a clay-dominated matrix, termed palagonite, which acts to cement the bulk rock. The abundant, hydrous phyllosilicate minerals within the palagonite can dehydrate at elevated temperatures, potentially resulting in thermal liability of the bulk rock. Using surficial samples collected from Krafla, northeast Iceland, and a range of petrographic, mineralogical, and mechanical analyses, we find that smectite dehydration occurs at temperatures commonly experienced within geothermal fields. Dehydration events at 130, 185, and 600°C result in progressive mass loss and contraction. This evolution results in a positive correlation between treatment temperature, porosity gain, and permeability increase. Gas permeability measured at 1 MPa confining pressure shows a 3-fold increase following thermal treatment at 600°C. Furthermore, strength measurements show that brittle failure is dependent on porosity and therefore the degree of thermal treatment. Following thermal treatment at 600°C, the indirect tensile strength, uniaxial compressive strength, and triaxial compressive strength (at 5 MPa confining pressure) decrease by up to 68% (1.1 MPa), 63% (7.3 MPa), and 25% (7.9 MPa), respectively. These results are compared with hyaloclastite taken from several depths within the Krafla reservoir, through which the palagonite transitions from smectite-to chlorite-dominated. We discuss how temperature-induced changes to the geomechanical properties of hyaloclastite may impact fluid flow in hydrothermal reservoirs and consider the potential implications for hyaloclastite-hosted intrusions. Ultimately, we show that phyllosilicate-bearing rocks are susceptible to temperature fluctuations in geothermal fields. © 2020 Josh Weaver et al

An Investigation of Clinical Correlates of Lewy Bodies in Autopsy-Proven Alzheimer Disease

Background: Studies of patients meeting clinical and pathologic criteria for Alzheimer disease (AD) have not consistently found associations between the presence of Lewy bodies (LBs) at postmortem examination and a higher frequency during life of the clinical features of dementia with LBs. Objective: To evaluate the clinical correlates of LBs in patients with AD. Design and Methods: Fifty-one patients were diagnosed as having probable AD during life and met pathologic criteria for AD. Semiquantitative ratings for LBs were obtained in 4 brain regions: substantia nigra, cingulate, insular cortex, and hippocampus. The patients had been followed up semiannually for up to 9.9 years before death, and clinical features associated with dementia with LBs, including extrapyramidal signs and visual hallucinations, were assessed at each study visit. Logistic regression analyses determined whether patients who had LBs were more likely than those without LBs to express specific clinical signs during follow-up. Cox analyses determined whether patients with LBs developed clinical signs or died earlier. Generalized estimating equations were used to compare rates of cognitive or functional change. Results: Nineteen of the 51 patients had at least 1 LB in one of the studied regions. In no case was a significant relation noted between LBs and the presence of a measured clinical sign. No LB measure was associated with an increased risk of developing any of the evaluated clinical signs earlier in the disease. There was no association between the presence of LBs and more rapid mortality or more rapid disease progression. Conclusions: In patients diagnosed as having AD during life, we did not observe a relation of LBs noted during postmortem examination with the presence of any clinical feature that we assessed or with the rapidity of disease progression. The relation between LBs and specific clinical manifestations may be tenuous in these patients

Towards the improvement of methane production in CO2 photoreduction using Bi2WO6/TiO2 heterostructures

Russelite bismuth tungstate (Bi2WO6) has been widely reported for the photocatalytic degradation and mineralization of a myriad of pollutants as well as organic compounds. These materials present perovskite-like structure with hierarchical morphologies, which confers excellent optoelectronic properties as potentials candidates for photocatalytic solar fuels production. Here, we propose the development of Bi2WO6/TiO2 heterojunctions for CO2 photoreduction, as a promising solution to produce fuels, alleviate global warming and tackle fossil fuel shortage. Our results show an improvement of the photocatalytic activity of the heterojunctions compared to the pristine semiconductors. Near Ambient Pressure X-ray Photoelectron Spectroscopy (NAP-XPS) experiments reveals a preferential CO2 adsorption over TiO2. On the other hand, transient absorption spectroscopy measurements show that the charge transfer pathway in Bi2WO6/TiO2 hybrids leads to longer-lived photogenerated carriers in spatially separated redox active sites, which favor the reduction of CO2 into highly electron demanding fuels and chemicals, such as CH4 and C2H6Financial support has been received from the European Research Council (ERC), through HYMAP project (grant agreement No. 648319), under the European Union's Horizon 2020 research and innovation program, as well as from the Marie Sklodowska-Curie grant agreement No. 754382. L.C. acknowledges funding from the project ARMONIA (PID2020–119125RJ-I00) funded by MCIN/AEI/10.13039/ 501100011033. Financial support has also been received from AEIMINECO/FEDER (Nympha Project, PID2019–106315RB-I00), "Comunidad de Madrid" regional government, and the European Structural Funds (FotoArt-CM project, S2018/NMT-4367). Authors also acknowledge financial support from the grant PLEC2021–007906 funded by MCIN/AEI/10.13039/501100011033 and the "European Union NextGenerationEU/PRTR"