600 research outputs found
An Ethernet-Based Fronthaul Implementation with MAC/PHY Split LTE Processing
A testbed implementation for an Ethernet fronthaul transporting signals arising from a long-term evolution (LTE) functional subdivision (“split”) at the media-access control (MAC)/physical layer (PHY) interface is presented. Based on open LTE base station software, the testbed demonstrates significant data rate reductions compared to current fronthaul implementations that rely on In-phase and Quadrature radio sample transportation and data rates that scale with cell load. All generated traffic flows are clearly distinguishable using appropriate packet headers. A selection of test cases and their corresponding results are presented to demonstrate the operation of the fronthaul and the performance of individual flows in terms of data rates and overheads
Development and validation of an in house multiplex real time PCR for quantification of all four serotypes of dengue virus
Objectives: In order to determine the infecting dengue virus (DENV) serotype and also to quantify the amount of virus in clinical samples and cell cultures, we proceeded to optimize and validate a quantitative real time RT-PCR assay.Methods: WHO reference strains of the four DENV serotypes were cultured on C6/36 cell lines for 7 days. The supernatants were used to determine plaque forming units (pfus) using BHK cell lines, which is indicative of infecting virus particles. cDNA was synthesized from RNA extracted from the virus culture supernatants. A tenfold dilution series (106 to 101 pfu/ml) of the known pfus of the viruses was prepared using the cDNA for standard curves. Data were analyzed using applied biosystems sequence detection systems. The threshold cycle value (Ct) for each reaction was determined by manually setting the threshold limit. Each standard curve was initially generated individually and later was used in a multiplex assay. All experiments were done in triplicate.Results: The optimized multiplex method detected all four DENV serotypes and generated standard curves with correlation coefficients (R2 values) above 0.95, which suggested that the standard curves and dilutions were of acceptable quality. The slope values of the standard curves were between -3.1 and -3.8 for all assays, implying that the PCR reactions were quite efficient.Conclusions: We have optimized and validated a multiplex quantitative real time RT-PCR assay, which can be effectively used to determine the infecting DENV in samples and also quantify the amount of virus
New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis
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The Spatial Properties of L- and M-Cone Inputs to Electroretinograms That Reflect Different Types of Post-Receptoral Processing
yesWe studied the spatial arrangement of L- and M-cone driven electroretinograms (ERGs) reflecting
the activity of magno- and parvocellular pathways. L- and M-cone isolating sine
wave stimuli were created with a four primary LED stimulator using triple silent substitution
paradigms. Temporal frequencies were 8 and 12 Hz, to reflect cone opponent activity, and
30, 36 and 48 Hz to reflect luminance activity. The responses were measured for full-field
stimuli and for different circular and annular stimuli. The ERG data confirm the presence of
two different mechanisms at intermediate and high temporal frequencies. The responses
measured at high temporal frequencies strongly depended upon spatial stimulus configuration.
In the full-field conditions, the L-cone driven responses were substantially larger than
the full-field M-cone driven responses and also than the L-cone driven responses with
smaller stimuli. The M-cone driven responses at full-field and with 70° diameter stimuli displayed
similar amplitudes. The L- and M-cone driven responses measured at 8 and 12 Hz
were of similar amplitude and approximately in counter-phase. The amplitudes were constant
for most stimulus configurations. The results indicate that, when the ERG reflects luminance
activity, it is positively correlated with stimulus size. Beyond 35° retinal eccentricity,
the retina mainly contains L-cones. Small stimuli are sufficient to obtain maximal ERGs at
low temporal frequencies where the ERGs are also sensitive to cone-opponent processin
Reconstruction of field theory from excitation spectra of defects
We show how to reconstruct a field theory from the spectrum of bound states
on a topological defect. We apply our recipe to the case of kinks in 1+1
dimensions with one or two bound states. Our recipe successfully yields the
sine-Gordon and field theories when suitable bound state
spectra are assumed. The recipe can also be used to globally reconstruct the
inflaton potential of inflationary cosmology if the inflaton produces a
topological defect. We discuss how defects can provide ``smoking gun'' evidence
for a class of inflationary models.Comment: 10 pages, 4 figures. Included proof (Appendix B) that wall
fluctuation potentials have supersymmetric form. Added reference
Field-scale assessment of the unsaturated hydraulic properties of residual soils in southeastern Brazil
Field tests were carried out to estimate effective unsaturated soil hydraulic properties of layered residual soils in Rio de Janeiro, southeastern Brazil. Data of this type are important for understanding the initiation of rainstorm-induced soil landslides, which often occur in the state of Rio de Janeiro as well as other areas having similar geologic settings and climate conditions. Tests were carried out using a simplified field approach, referred to as the Monitored Infiltration Test, which requires only a tensiometer to measure pressure heads below the wetting front, triggered by flow from a Mariotte bottle which maintains a constant pressure at the top edge of the soil profile. The data can then be analyzed by numerical inversion using the HYDRUS-2D software package. The test is relatively fast since no steady-state flow conditions are needed, and versatile since the test can be carried out quickly on steep slopes with the help of a manual auger. Soil water retention and the unsaturated hydraulic conductivity functions were obtained for a range of young, mature and saprolitic residual soils. The effective hydraulic properties of the distinct residual soil layers can be quite large, reflecting a need to provide a careful analysis of field-scale hydraulic heterogeneity in geotechnical analyses
Comparison of the reliability of multifocal visual evoked cortical potentials generated by pattern reversal and pattern pulse stimulation
This study compared the effectiveness of the multifocal visual evoked cortical potentials (mfVEP) elicited by pattern pulse stimulation with that of pattern reversal in producing reliable responses (signal-to-noise ratio >1.359). Participants were 14 healthy subjects. Visual stimulation was obtained using a 60-sector dartboard display consisting of 6 concentric rings presented in either pulse or reversal mode. Each sector, consisting of 16 checks at 99% Michelson contrast and 80 cd/m² mean luminance, was controlled by a binary m-sequence in the time domain. The signal-to-noise ratio was generally larger in the pattern reversal than in the pattern pulse mode. The number of reliable responses was similar in the central sectors for the two stimulation modes. At the periphery, pattern reversal showed a larger number of reliable responses. Pattern pulse stimuli performed similarly to pattern reversal stimuli to generate reliable waveforms in R1 and R2. The advantage of using both protocols to study mfVEP responses is their complementarity: in some patients, reliable waveforms in specific sectors may be obtained with only one of the two methods. The joint analysis of pattern reversal and pattern pulse stimuli increased the rate of reliability for central sectors by 7.14% in R1, 5.35% in R2, 4.76% in R3, 3.57% in R4, 2.97% in R5, and 1.78% in R6. From R1 to R4 the reliability to generate mfVEPs was above 70% when using both protocols. Thus, for a very high reliability and thorough examination of visual performance, it is recommended to use both stimulation protocols
Sphingosine 1-Phosphate in acute dengue infection
Objectives: Many mediators have been implicated in the vascular leak in dengue which is a hallmark of severe dengue. Sphingosine 1-Phosphate (S1P) has been shown to counteract the effects of other mediators that cause increase vascular permeability. Moreover, S1P has been shown to be important in barrier integrity. Therefore, we investigated the role of S1P in acute dengue. This has not been investigated previously.Methods: Serum samples from acute dengue patients were collected 12 hours apart throughout the course of their hospital stay. S1P levels in 32 patients with acute dengue and 12 healthy individuals were assessed using ELISA.Results: S1P levels were significantly lower in patients with acute dengue (p=0.002) and the levels in patients with DHF were significantly lower than those with dengue fever (p=0.005). S1P levels were low throughout the course of illness and S1P levels were < 0.5 μM in 12/23 patients with DHF when compared to 1/9 with dengue fever (DF). Majority of patients with DHF had lower S1P levels especially in the critical phase. Some patients with DF also had quite low levels at certain time points during the course of the illness. The serial S1P levels in patients with both DF and DHF significantly correlated with the serial platelet counts (Spearmans r =0.18, p=0.04).Conclusions: Low levels of S1P in acute dengue infection are likely to contribute to increased vascular permeability. Therefore S1P analogue may have a place in the treatment of acute dengue
Correlation of serum leptin and insulin levels of pregnant protein-restricted rats with predictive obesity variables
During pregnancy and protein restriction, changes in serum insulin and leptin levels, food intake and several metabolic parameters normally result in enhanced adiposity. We evaluated serum leptin and insulin levels and their correlations with some predictive obesity variables in Wistar rats (90 days), up to the 14th day of pregnancy: control non-pregnant (N = 5) and pregnant (N = 7) groups (control diet: 17% protein), and low-protein non-pregnant (N = 5) and pregnant (N = 6) groups (low-protein diet: 6%). Independent of the protein content of the diet, pregnancy increased total (F1,19 = 22.28, P < 0.001) and relative (F1,19 = 5.57, P < 0.03) food intake, the variation of weight (F1,19 = 49.79, P < 0.000) and final body weight (F1,19 = 19.52, P < 0.001), but glycemia (F1,19 = 9.02, P = 0.01) and the relative weight of gonadal adipose tissue (F1,19 = 17.11, P < 0.001) were decreased. Pregnancy (F1,19 = 18.13, P < 0.001) and low-protein diet (F1,19 = 20.35, P < 0.001) increased the absolute weight of brown adipose tissue. However, the relative weight of this tissue was increased only by protein restriction (F1,19 = 15.20, P < 0.001) and the relative lipid in carcass was decreased in low-protein groups (F1,19 = 4.34, P = 0.05). Serum insulin and leptin levels were similar among groups and did not correlate with food intake. However, there was a positive relationship between serum insulin levels and carcass fat depots in low-protein groups (r = 0.37, P < 0.05), while in pregnancy serum leptin correlated with weight of gonadal (r = 0.39, P < 0.02) and retroperitoneal (r = 0.41, P < 0.01) adipose tissues. Unexpectedly, protein restriction during 14 days of pregnancy did not alter the serum profile of adiposity signals and their effects on food intake and adiposity, probably due to the short term of exposure to low-protein diet.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Foundations FAPEMATUniversidade Federal de Mato Grosso Faculdade de Ciências MédicasUniversidade Federal de Mato Grosso Faculdade de Nutrição Departamento de Alimentos e NutriçãoUniversidade Estadual de Campinas Instituto de Biologia Departamento de Fisiologia e BiofísicaUniversidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia da NutriçãoUNIFESP, Depto. de Fisiologia da NutriçãoBrazilian Foundations FAPEMAT: 175/04CNPq: 479138/2003-6SciEL
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