Producción de látex híbridos acrílicos/alquídicos

In this work, the production of high solids content hybrid acrylic/alkyd latexes by miniemulsion polymerization is discussed. First, the miniemulsification procedure to achieve colloidally stable hybrid nanodroplets is presented. Next, the efficient nucleation of most nanodroplets during the polymerization, avoiding other nucleation mechanisms is presented. Finally, the key aspects to control the polymer architecture as well as the particle morphology are analyzed.Fil: Goikoetxea, Monika. Universidad del País Vasco; EspañaFil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Beristain, Itxaso. Universidad del País Vasco; EspañaFil: Paulis, María. Universidad del País Vasco; EspañaFil: Asua, José M.. Universidad del País Vasco; EspañaFil: Barandiaran, María J.. Universidad del País Vasco; Españ

SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer

© 2024 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).The posttranslational modification of proteins critically influences many biological processes and is a key mechanism that regulates the function of the RNA-binding protein Hu antigen R (HuR), a hub in liver cancer. Here, we show that HuR is SUMOylated in the tumor sections of patients with hepatocellular carcinoma in contrast to the surrounding tissue, as well as in human cell line and mouse models of the disease. SUMOylation of HuR promotes major cancer hallmarks, namely proliferation and invasion, whereas the absence of HuR SUMOylation results in a senescent phenotype with dysfunctional mitochondria and endoplasmic reticulum. Mechanistically, SUMOylation induces a structural rearrangement of the RNA recognition motifs that modulates HuR binding affinity to its target RNAs, further modifying the transcriptomic profile toward hepatic tumor progression. Overall, SUMOylation constitutes a mechanism of HuR regulation that could be potentially exploited as a therapeutic strategy for liver cancer.This work was supported by grants to M.L.M.-C. from Departamento de Industria del Gobierno Vasco, Spain; Ministerio de Ciencia e Innovación, Spain (grant no. PID2020-117116RB-I00); European Regional Development Fund (ERDF), EU; and CIBERehd, which is funded by Instituto de Salud Carlos III (ISCIII), Spain. M.L.M.-C. and J.S. received funding from Ministerio de Ciencia e Innovación (grant no. RTC2019-007125-1) and ISCIII (grant no. DTS20/00138). M.L.M.-C. and R.M.L. acknowledge Ministerio de Ciencia e Innovación (grant no. RED2022-134397-T). M.L.M.-C. and J.M.B. were awarded with a grant from Fundación la Caixa, Spain (grant no. HR17-00601). M.L.M.-C., J.M.B., M.A.A., and J.J.G.M. acknowledge financial support from Fundación Científica de la Asociación Española Contra el Cáncer (AECC), Spain. M.S.R. recognizes funding from Fondo Sectorial de Investigación SRE - CONACYT, Mexico (grant no. 0280365); Horizon 2020 Research and Innovation Program funded under Marie Skłodowska-Curie Actions, EU (grant no. 765445); and REPÈRE and Programme de Prématuration from Région Occitanie, France. M.G., S.D., and K.M.-M. were supported by the National Institute on Aging (NIA), National Institutes of Health (NIH), US (grant no. Z01-AG000511-23). I.D.-M. is grateful for the grants received from Junta de Andalucía, Spain (grant no. BIO-198, US-1254317, P18-FR-3487, and P18-HO-4091); Ministerio de Ciencia, Innovación y Universidades, Spain (grant no. PGC2018-096049-BI00); and Fundación Ramón Areces, Spain. T.D. acknowledges Fondation ARC, France (grant no. 208084). J.J.G.M. was supported by Junta de Castilla y León, Spain (grant no. SA063P17); Fundación La Marató TV3, Spain (grant no. 201916-31); ISCIII (grant no. PI19/00819); CIBERehd; and ERDF (grant no. OLD-HEPAMARKER). M.A.A. recognizes Gobierno de Navarra, Spain (grant no. GºNa 42/21); Eurorregión Nueva Aquitania-Euskadi-Navarra, Spain; Ministerio de Ciencia e Innovación (grant no. PID2019-104878RB-I00); and CIBERehd. A.P. expresses gratitude to the European Research Council (ERC), EU (grant no. 804236) for their support. M.D.G. received financial support from Junta de Andalucía (grant no. PEMP-0036-2020 and BIO-0139); Ministerio de Universidades, Spain (grant no. FPU20/03957); ISCIII (grant no. PI20/01301), Fundación Sociedad Española de Endocrinología y Nutrición (FSEEN), Spain; CIBERehd; and CIBERobn, which is also funded by ISCIII. J.M.B. acknowledges Euskadi RIS3 (grant no. 2019222054, 2020333010, and 2021333003) and Elkartek programs from Gobierno Vasco (grant no. KK-2020/00008); ISCIII (grant no. PI18/01075, CPII19/00008, and PI21/00922); CIBERehd; PSC Support, UK; AMMF The Cholangiocarcinoma Charity, UK (grant no. EU/2019/AMMFt/001); Horizon 2020 Research and Innovation Program (grant no. 825510); ERDF; and PSC Partners Seeking a Cure, US. A.L. received financial support from the Damon Runyon-Rachleff Innovation Award, US (grant no. DR52-18) and the MERIT Award (R37) from the National Cancer Institute (NCI), NIH (grant no. R37CA230636). F.E. expresses his gratitude to ProteoRed from ISCIII (grant no. PT13/0001/0027) and CIBERehd. N.G.A.A. was funded by Ministerio de Ciencia, Innovación y Universidades (grant no. RTI2018-095700-B-I00). R.B. acknowledges financial support from Gobierno Vasco (grant no. IT1165-19); Ministerio de Economía, Industria y Competitividad, Spain (grant no. SAF2017-90900-REDT); Ministerio de Economía, Industria y Competitividad, ERDF (grant no. BFU2017-84653-P); Ministerio de Ciencia e Innovación (grant no. PID2020-114178GB-I00); and Horizon 2020 funded under Marie Skłodowska-Curie Actions (grant no. 765445-EU). A.M.A. acknowledges CIBERehd. L.A.M.-C. obtained grants from Ministerio de Economía y Competitividad (grant no. CSD2008-00005); Ministerio de Economía, Industria y Competitividad (grant no. BFU2016-77408-R); ISCIII; and EJP RD, EU (grant no. EJPRD19-040). I.G.-R. was supported by Ministerio de Economía, Industria y Competitividad (grant no. BES-2017-080435 ). M.S.-M. is grateful to the AECC, Sede de Bizkaia, Spain for the financial support. J.D.Z. was awarded with a grant from Ministerio de Economía, Industria y Competitividad (grant no. SEV-2016-0644-18-2). C.M. acknowledges Gobierno Vasco (grant no. IT-1264-19) and Ministerio de Ciencia e Innovación (grant no. PID2022-136788OB-I00). A.V.-C. was supported by Ministerio de Educación, Cultura y Deporte, Spain (grant no. FPU016/01513). C.F.-R. thanks Tekniker, Spain and CIC bioGUNE, Spain for financial support. A.G.-d.R. was funded by Bikaintek program from Gobierno Vasco (grant no. 48-AF-W1-2019-00012). N.G.-U. obtained a grant from Gobierno Vasco. T.C.D. expresses gratitude to AECC. J.S. received financial support from CIBERehd. C.M.R.-G. was supported by Ayudas a la Recualificación Margarita Salas from Universidad de Extremadura, Ministerio de Universidades financed by NextGenerationEU.Peer reviewe

Hyperspectral infrared nanoimaging of organic samples based on Fourier transform infrared nanospectroscopy

Infrared nanospectroscopy enables novel possibilities for chemical and structural analysis of nanocomposites, biomaterials or optoelectronic devices. Here we introduce hyperspectral infrared nanoimaging based on Fourier transform infrared nanospectroscopy with a tunable bandwidth-limited laser continuum. We describe the technical implementations and present hyperspectral infrared near-field images of about 5,000 pixel, each one covering the spectral range from 1,000 to 1,900 cm−1. To verify the technique and to demonstrate its application potential, we imaged a three-component polymer blend and a melanin granule in a human hair cross-section, and demonstrate that multivariate data analysis can be applied for extracting spatially resolved chemical information. Particularly, we demonstrate that distribution and chemical interaction between the polymer components can be mapped with a spatial resolution of about 30 nm. We foresee wide application potential of hyperspectral infrared nanoimaging for valuable chemical materials characterization and quality control in various fields ranging from materials sciences to biomedicine

Subsurface chemical nanoidentification by nano-FTIR spectroscopy

Nano-FTIR spectroscopy allows chemical characterization of composite surfaces, but its capability in subsurface analysis is not much explored. The authors show that spectra from thin surface layers differ from those of subsurface layers of the same organic material, and establish a method for distinguishing them in experiments

Continuous miniemulsification for the industrialization of miniemulsion polymerization

Miniemulsion polymerization allows the synthesis of a broad range of materials, which are not obtainable by means of other polymerization processes in dispersed media such as conventional emulsion polymerization. However, the industrial implementation of the miniemulsion polymerization is being hindered by the miniemulsification process, which may require long times leading to unacceptable process times. This work explores the feasibility of using a continuous miniemulsification system that includes a static mixer and a high pressure homogenizer in series, in both loop arrangement and single pass. The proposed system allows the production at high rates, of high solids content miniemulsions containing a very viscous organic phase.Fil: Goikoetxea, Monika. Universidad del Pais Vasco; EspañaFil: Beristain, Itxaso. Universidad del Pais Vasco; EspañaFil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad del Pais Vasco; EspañaFil: Paulis, María. Universidad del Pais Vasco; EspañaFil: Barandiaran, María. Universidad del Pais Vasco; EspañaFil: Asua, José. Universidad del Pais Vasco; Españ

A New Strategy to Improve Alkyd/Acrylic Compatibilization in Waterborne Hybrid Dispersions

Grafting of alkyd resin to the acrylic polymer is needed to fully exploit the possibilities of these hybrids; but in the current technology, grafting is accompanied by an important reduction of the unsaturated groups of the alkyds, which adversely affects the curing of the film and its properties. A novel strategy that overcomes these limitations is presented. The strategy is based on the use of alkyd resin functionalized with methacrylic groups, that substantially improved the incorporation of the resin to the growing acrylic polymer chain, and the polymer particle homogeneity, without decreasing the degree of unsaturation in the alkyd resin.Fil: Goikoetxea, Monika. Universidad del Pais Vasco; EspañaFil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad del Pais Vasco; EspañaFil: Beristain, Itxaso. Universidad del Pais Vasco; EspañaFil: Paulis, María. Universidad del Pais Vasco; EspañaFil: Barandiaran, María. Universidad del Pais Vasco; EspañaFil: Asua, José M.. Universidad del Pais Vasco; Españ

Post-polymerization of waterborne alkyd/acrylics. Effect on polymer architecture and particle morphology

The limiting conversion commonly attained in the synthesis of waterborne alkyd/acrylic hybrid latexes hinders their industrialization. In the first part of this work, the mechanisms causing the limiting conversion are studied and post-polymerization conditions to efficiently remove the residual monomer determined. Both the polymer architecture and the particle morphology were modified. Therefore, in the second part the mechanisms involved in this modification were investigated in an attempt to transform a potential threat for the material properties in an opportunity to expand the range of properties achievable.Fil: Minari, Roque Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Goikoetxea, Monika. Universidad del País Vasco; EspañaFil: Beristain, Itxaso. Universidad del País Vasco; EspañaFil: Paulis, María. Universidad del País Vasco; EspañaFil: Barandiaran, María J.. Universidad del País Vasco; EspañaFil: Asua, José M.. Universidad del País Vasco; Españ