Possible magnetic field variability during the 6.7 GHz methanol maser flares of G09.62+0.20

(Abridged) Recently, the magnetic field induced Zeeman splitting was measured for the strongest known 6.7 GHz methanol maser, which arises in the massive star forming region G09.62+0.20. This maser is one of a handful of periodically flaring methanol masers. The 100-m Effelsberg telescope was used to monitor the 6.7 GHz methanol masers of G09.62+0.20. With the exception of a two week period during the peak of the maser flare, we measure a constant magnetic field of B_||~11+-2 mG in the two strongest maser components of G09.62+0.20 that are separated by over 200 AU. In the two week period that coincides exactly with the peak of the maser flare of the strongest maser feature, we measure a sharp decrease and possible reversal of the Zeeman splitting. The exact cause of both maser and polarization variability is still unclear, but it could be related to either background amplification of polarized emission or the presence of a massive protostar with a close-by companion. Alternatively, the polarization variability could be caused by non-Zeeman effects related to the radiative transfer of polarized maser emission.Comment: 4 pages, 3 figures, accepted for publication Astronomy and Astrophysic

The effect of canopy position on growth and mortality in mixed sapling communities during self-thinning

This research investigates how species in the sapling phase differ in growth and survival depending on light availability (as estimated by canopy position) by means of tree-ring analysis and modelling mortality. We harvested 120 live and 158 dead saplings in self-thinning communities consisting of Silver birch (Betula pendula Roth.), Scots pine (Pinus sylvestris L.), Japanese larch (Larix kaempferi Carr.) and Douglas fir (Pseudotsuga menziesii Mirb. Franco) in the Netherlands. Results are evaluated within the framework of a trade-off between high-light growth and low-growth survival. Radial growth, measured at ground level, generally declined over time. In addition, a decreasing light availability further reduced growth in all species except Douglas fir. Trees died when radial growth was reduced to about 0.5 mm year¿1. Mortality in all species except Scots pine was significantly related to recent growth, but mortality curves were not different. The light-demanding Silver birch and Japanese larch differed from the shade-tolerant Douglas fir in both high-light growth and low-growth mortality, in line with a growth-survival trade-off. The light-demanding Scots pine did not fit this pattern as it was unable to transfer high radial growth into height gain, leaving it in suppressed canopy positions. This indicates the importance of height growth in the growth-survival trade-off. Differences in mortality probabilities affect the potential for coexistence, however, in all species also fast-growing individuals died suggesting additional factors causing mortality during self-thinning, other than direct competition for ligh

Maintenance of memory CD8 T cells: Divided over division

Once generated during an infection, memory CD8(+) T cells can provide long-lasting protection against reinfection with an intracellular pathogen, but the longevity of this defense depends on the ability of these pathogen-specific memory cells to be maintained. It is generally believed that the bone marrow plays an important role in this respect, where memory CD8 T cells receive reinvigorating signals from cytokines that induce homeostatic proliferation. However, in the current issue of the European Journal of Immunology, Siracusa et al. (Eur. J. Immunol. 2017. 47: 1900-1905) argue against this dogma, as they provide evidence that CD8 memory T cells in murine bone marrow are not proliferating, but largely quiescent, which protects them from elimination by the cytostatic drug Cyclophosphamide. Interestingly, this is in sharp contrast to the proliferating cell counterparts in the spleen, which are eliminated by this treatment. Here, we will discuss the impact of these results, how they relate to opposing findings by others in the field, and what the relevance of these findings is for humans and clinical applications

VLBA imaging of a periodic 12.2 GHz methanol maser flare in G9.62+0.20E

The class II methanol maser source G9.62+0.20E undergoes periodic flares at both 6.7 and 12.2 GHz. The flare starting in 2001 October was observed at seven epochs over three months using the VLBA at 12.2 GHz. High angular resolution images (beam size $\sim$ 1.7 x 0.6 mas) were obtained, enabling us to observe changes in 16 individual maser components. It was found that while existing maser spots increased in flux density, no new spots developed and no changes in morphology were observed. This rules out any mechanism which disturbs the masing region itself, implying that the flares are caused by a change in either the seed or pump photon levels. A time delay of 1--2 weeks was observed between groups of maser features. These delays can be explained by light travel time between maser groups. The regularity of the flares can possibly be explained by a binary system.Comment: 11 pages, accepted for publication in MNRA

Growth and Lipid Content of Osmo-Tolerant and Sensitive Algae

Drought tolerance in a number of crop species has been correlated with changes in and composition of cell membrane lipids. Since membrane stability is a function of the physical properties of the lipid constituents, recent studies have focused on lipid changes in response to natural and artifically-induced water stress. Such studies have clearly shown that lipid adjustment and composition differs in drought-resistant and -tolerant species. A difficulty in working with higher plant systems is that vascular plants make structural and functional level adjustments which aid in drought resistance and which affect the process of adjustment at the cellular level. For this reason, in vitro cell culture techniques have many advantages in studying stress responses. Thus, the objectives of this research were to determine the effects of polyethylene glycol (PEG) -induced osmotic stress on the lipid and sterol composition of osmotic tolerant and susceptible species of unicellular algae belonging to the genus Chlorella

PlotXpress, a webtool for normalization and visualization of reporter expression data [version 1; peer review: 3 approved]

In molecular cell biology, reporter assays are frequently used to investigate gene expression levels. Reporter assays employ a gene that encodes a light-emitting protein, of which the luminescence is quantified as a proxy of gene expression. Commercial parties provide reporter assay kits that include protocols and specialized detection machinery. However, downstream analysis of the output data and their presentation are not standardized. We have developed plotXpress to fill this gap, providing a free, open-source platform for the semi-automated analysis and standardized visualisation of experimental gene reporter data. Users can upload raw luminescence data acquired from a reporter gene assay with an internal control. In plotXpress, the data is corrected for sample variation with the internal control and the average for each condition is calculated. When a reference condition is selected the fold change is calculated for all other conditions, based on the selected reference. The results are shown as dot plots with a statistical summary, which can be adjusted to create publication-grade plots without requiring coding skills. Altogether, plotXpress is an open-source, low-threshold, web-based tool, that promotes a standardized and reproducible analysis while providing an appealing visualization of reporter data. The webtool can be accessed at: https://huygens.science.uva.nl/PlotXpress

Periodic class II methanol masers in G9.62+0.20E

We present the light curves of the 6.7 and 12.2 GHz methanol masers in the star forming region G9.62+0.20E for a time span of more than 2600 days. The earlier reported period of 244 days is confirmed. The results of monitoring the 107 GHz methanol maser for two flares are also presented. The results show that flaring occurs in all three masing transitions. It is shown that the average flare profiles of the three masing transitions are similar. The 12.2 GHz masers are the most variable of the three masers with the largest relative amplitude having a value of 2.4. The flux densities for the different masing transitions are found to return to the same level during the low phase of the masers, suggesting that the source of the periodic flaring is situated outside the masing region, and that the physical conditions in the masing region are relatively stable. On the basis of the shape of the light curve we excluded stellar pulsations as the underlying mechanism for the periodicity. It is argued that a colliding wind binary can account for the observed periodicity and provide a mechanism to qualitatively explain periodicity in the seed photon flux and/or the pumping radiation field. It is also argued that the dust cooling time is too short to explain the decay time of about 100 days of the maser flare. A further analysis has shown that for the intervals from days 48 to 66 and from days 67 to 135 the decay of the maser light curve can be interpreted as due to the recombination of a thermal hydrogen plasma with densities of approximately $1.6 \times 10^6 \mathrm{cm^{-3}}$ and $6.0 \times 10^5 \mathrm{cm^{-3}}$ respectively.Comment: 11 pages, 9 figuer

KAT-7 Science Verification: Using HI Observations of NGC 3109 to Understand its Kinematics and Mass Distribution

HI observations of the Magellanic-type spiral NGC 3109, obtained with the seven dish Karoo Array Telescope (KAT-7), are used to analyze its mass distribution. Our results are compared to what is obtained using VLA data. KAT-7 is the precursor of the SKA pathfinder MeerKAT, which is under construction. The short baselines and low system temperature of the telescope make it sensitive to large scale low surface brightness emission. The new observations with KAT-7 allow the measurement of the rotation curve of NGC 3109 out to 32', doubling the angular extent of existing measurements. A total HI mass of 4.6 x 10^8 Msol is derived, 40% more than what was detected by the VLA observations. The observationally motivated pseudo-isothermal dark matter (DM) halo model can reproduce very well the observed rotation curve but the cosmologically motivated NFW DM model gives a much poorer fit to the data. While having a more accurate gas distribution has reduced the discrepancy between the observed RC and the MOdified Newtonian Dynamics (MOND) models, this is done at the expense of having to use unrealistic mass-to-light ratios for the stellar disk and/or very large values for the MOND universal constant a0. Different distances or HI contents cannot reconcile MOND with the observed kinematics, in view of the small errors on those two quantities. As for many slowly rotating gas-rich galaxies studied recently, the present result for NGC 3109 continues to pose a serious challenge to the MOND theory.Comment: 25 pages, 20 figures, accepted for publication in Astronomical Journa