Artemisia thuscula Cav.: antibacterial, antifungal activity of the plant extracts and associated endophytes

4 páginas, 2 tablas.In this paper we are presenting preliminary results for the antifungal and antibacterian activity of the Artemisia thuscula Cav. all together with the endophytic communities encountered in symbiosis with this specie. This plant is endemic for the Canary Islands and it is recognised for its traditional medicinal use (like other species of the same genus in the rest of the world) and for being a functional repellent of insects. The ethanol extracts tested showed an interesting activity against the phytopathogenic fungi Fusarium monilforme, F. solani and F. oxysporum and antibiotic activity against 2 Gram-positive bacteria: Bacillus cereus and Streptomyces griseus, in an primary screening. The diversity of endophytes found in this plant, especially in the roots, showed promising results supporting further work on this species.This publication has been financed by the project: ‘Bioprospection of endophytes in medicinal plants for biopesticides production’ MCIN- FCCI. 2009. Ref. ACI2009-0900Peer reviewe

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Fungal endophytes and their role in plant protection

14 pages, 10 schemes.-- Full-text version available at: http://mail.fq.edu.uy/~leq/Interacciones_posgrado/Referencias/Gimenez2007endophytes.pdfEndophytes (fungal and bacterial organisms) have attracted great interest over the past few years because their presence benefits the host plant (development and defence) and they are a source of secondary metabolites of potential interest and thus play an important role in the regulation of plant communities and their herbivores. There are an increasing number of reports on their identification and on the production of secondary metabolites. Their role in plant-pathogen and plant-insect interactions is receiving increasing attention because of their potential use in pest control, however, little is known about their physiology and the regulation processes of the plant-endophyte interaction.This work was partially supported by grants MCYT (BQU2001-1505) and CAM (07M/0073/2002)

Endophytic fungi isolated from musa acuminata “Dwarf Cavendish” and their activity against phytopathogenic fungi

Copyright © 2016Endophytic fungi were isolated from Musa acuminata ‘Dwarf Cavendish’ harvested from 100-year-old crops. The antagonistic bioactivity and the inhibitory activity of the endophytic fungi were evaluated against various phytopathogenic fungi: Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen, F. oxysporum Schlect. f.sp. cubense (E.F. Smith) Snyd. & Hans, F. moniliforme (Sheldon), Alternaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp. and Cladosporium sp. In dual culture assays, endophytic strains of Penicillium, Aspergillus, Fusarium and Chaetomium were found to be active against some of the targeted microorganisms. Four active extracts were fractionised using VLC (vacuum liquid chromatography) and two ethyl acetate and ethanolic extracts obtained from Aspergillus oryzae isolate led to two active hexanic and ethyl acetate fractions which inhibited growth of F. moniliforme (%I = 48.02 and 55.1, respectively; at 1 mg ml−1). The smallest EC50 (0.74 mg ml−1) was observed in an extract obtained from a strain of Penicillium sp. The same endophytic strain inhibited F. oxysporum by 59.72% in VOC (volatile organic compounds) assays.The authors would like to thank ‘Catedra Cajamar del Sector Agroalimentario de la ULL’ (Universidad de La Laguna, Tenerife, Spain) for their support.Peer Reviewe

Bioactive eremophilanolides from Senecio poepigii

7 pages, 2 figures, 3 tables.-- PMID: 16286302 [PubMed].A new bioactive eremophilanolide, 1α-tigloyloxy-8βH,10βH-eremophil-7(11)-en-8α,12-olide (1), was isolated from Senecio poepigii and its structure was elucidated by spectral analysis. 1α-Angeloyloxy-8β-methoxy-10βH-eremophil-7(11)-en-8α,12-olide (2) was also isolated. Antifungal and insect antifeedant properties were evaluated.This work has been partially supported by grants from the DGICYT – Spain (BQU2001-1505), CYTED IV.13, DICYT (Universidad de Santiago de Chile, 020041V) and a Collaborative Research Grant from CSIC-USACH. C.G.M. and D.M-D.D have been supported by a predoctoral fellowship from the MCYT – Spain and Government of Canary Island – CSIC, respectively

Antifungal activity and chemical composition of endophytic microextracts from Bethencourtia palmensis

Trabajo presentado en la XXXVI Reunión Bienal de la Real Sociedad Española de Química ,celebrada en Sitges (España), del 25 al 29 de junio de 2017.Peer Reviewe

Natural broad-spectrum biocides

[ES] Biocidas naturales de amplio espectro procedentes del hongo endofito stemphylium solani. En el campo de la agricultura los organismos perjudiciales para la vegetación constituyen un importante problema en relación con la productividad de las cosechas. La presente invención se relaciona con un producto de fermentación obtenido a partir de hongos endófitos de la familia Sthemphylum solani, que puede comprender un nuevo compuesto de fórmula (I) y un segundo compuesto conocido de fórmula (II), y que presenta actividad biocida simultáneamente contra más de una categoría de organismos perjudiciales que afectan a plantas y que preferentemente se seleccionan entre insectos-plaga, hongos y nematodos. Tanto el producto de fermentación, como los compuestos incluidos en el ámbito de la invención, se usan en la elaboración de una composición biocida de amplio espectro. Finalmente, la invención también incluye el procedimiento de obtención del producto de fermentación y el nuevo compuesto de fórmula (I).[EN] In the field of agriculture, organisms that are harmful to vegetation are a major problem in relation to the productivity of harvests. The present invention relates to a fermentation product obtained from endophytic fungi of the Stemphylium solani family, which may comprise a novel compound of formula (I) and a second known compound of formula (II), and having biocidal activity simultaneously against more than one category of harmful organisms affecting plants, and which are preferably selected from insect-pests, fungi and nematodes. Both the fermentation product, and the compounds included in the scope of the invention, are used in the preparation of a broad-spectrum biocidal composition. Finally, the invention also includes the process for preparing of the fermentation product and the new compound of formula (I).Peer reviewedConsejo Superior de Investigaciones Científicas (España), Universidad de La Laguna, The Energy and the Resources InstituteA2 Solicitud de patente sin informe sobre el estado de la técnic

Natural broad-spectrum biocides

[EN] In the field of agriculture, organisms that are harmful to vegetation are a major problem in relation to the productivity of harvests. The present invention relates to a fermentation product obtained from endophytic fungi of the Stemphylium solani family, which may comprise a novel compound of formula (I) and a second known compound of formula (II), and having biocidal activity simultaneously against more than one category of harmful organisms affecting plants, and which are preferably selected from insect-pests, fungi and nematodes. Both the fermentation product, and the compounds included in the scope of the invention, are used in the preparation of a broad-spectrum biocidal composition. Finally, the invention also includes the process for preparing of the fermentation product and the new compound of formula (I).[ES] Biocidas naturales de amplio espectro procedentes del hongo endofito stemphylium solani. En el campo de la agricultura los organismos perjudiciales para la vegetación constituyen un importante problema en relación con la productividad de las cosechas. La presente invención se relaciona con un producto de fermentación obtenido a partir de hongos endófitos de la familia Sthemphylum solani, que puede comprender un nuevo compuesto de fórmula (I) y un segundo compuesto conocido de fórmula (II), y que presenta actividad biocida simultáneamente contra más de una categoría de organismos perjudiciales que afectan a plantas y que preferentemente se seleccionan entre insectos-plaga, hongos y nematodos. Tanto el producto de fermentación, como los compuestos incluidos en el ámbito de la invención, se usan en la elaboración de una composición biocida de amplio espectro. Finalmente, la invención también incluye el procedimiento de obtención del producto de fermentación y el nuevo compuesto de fórmula (I).Peer reviewedConsejo Superior de Investigaciones Científicas (España), Universidad de La Laguna, The Energy and the Resources InstituteA1 Solicitud de patente con informe sobre el estado de la técnic

Biocidas naturales de amplio espectro procedentes del hongo endofito stemphylium solani

[EN] In the field of agriculture, organisms that are harmful to vegetation are a major problem in relation to the productivity of harvests. The present invention relates to a fermentation product obtained from endophytic fungi of the Stemphylium solani family, which may comprise a novel compound of formula (I) and a second known compound of formula (II), and having biocidal activity simultaneously against more than one category of harmful organisms affecting plants, and which are preferably selected from insect-pests, fungi and nematodes. Both the fermentation product, and the compounds included in the scope of the invention, are used in the preparation of a broad-spectrum biocidal composition. Finally, the invention also includes the process for preparing of the fermentation product and the new compound of formula (I).[ES] Biocidas naturales de amplio espectro procedentes del hongo endofito stemphylium solani. En el campo de la agricultura los organismos perjudiciales para la vegetación constituyen un importante problema en relación con la productividad de las cosechas. La presente invención se relaciona con un producto de fermentación obtenido a partir de hongos endófitos de la familia Sthemphylum solani, que puede comprender un nuevo compuesto de fórmula (I) y un segundo compuesto conocido de fórmula (II), y que presenta actividad biocida simultáneamente contra más de una categoría de organismos perjudiciales que afectan a plantas y que preferentemente se seleccionan entre insectos-plaga, hongos y nematodos. Tanto el producto de fermentación, como los compuestos incluidos en el ámbito de la invención, se usan en la elaboración de una composición biocida de amplio espectro. Finalmente, la invención también incluye el procedimiento de obtención del producto de fermentación y el nuevo compuesto de fórmula (I).Peer reviewedConsejo Superior de Investigaciones Científicas (España), Universidad de La Laguna, The Energy and the Resources InstituteE Solicitud de patente europe

Natural broad-spectrum biocides

[ES] Biocidas naturales de amplio espectro procedentes del hongo endofito stemphylium solani. En el campo de la agricultura los organismos perjudiciales para la vegetación constituyen un importante problema en relación con la productividad de las cosechas. La presente invención se relaciona con un producto de fermentación obtenido a partir de hongos endófitos de la familia Sthemphylum solani, que puede comprender un nuevo compuesto de fórmula (I) y un segundo compuesto conocido de fórmula (II), y que presenta actividad biocida simultáneamente contra más de una categoría de organismos perjudiciales que afectan a plantas y que preferentemente se seleccionan entre insectos-plaga, hongos y nematodos. Tanto el producto de fermentación, como los compuestos incluidos en el ámbito de la invención, se usan en la elaboración de una composición biocida de amplio espectro. Finalmente, la invención también incluye el procedimiento de obtención del producto de fermentación y el nuevo compuesto de fórmula (I).[EN] In the field of agriculture, organisms that are harmful to vegetation are a major problem in relation to the productivity of harvests. The present invention relates to a fermentation product obtained from endophytic fungi of the Stemphylium solani family, which may comprise a novel compound of formula (I) and a second known compound of formula (II), and having biocidal activity simultaneously against more than one category of harmful organisms affecting plants, and which are preferably selected from insect-pests, fungi and nematodes. Both the fermentation product, and the compounds included in the scope of the invention, are used in the preparation of a broad-spectrum biocidal composition. Finally, the invention also includes the process for preparing of the fermentation product and the new compound of formula (I).Peer reviewedConsejo Superior de Investigaciones Científicas (España), Universidad de La Laguna, The Energy and the Resources InstituteB1 Patente sin examen previ