Effects of environmental factors on development of Pyrenopeziza brassicae (light leaf spot) apothecia on oilseed rape debris

Publication no. P-2001-0221-01R. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2001The development of Pyrenopeziza brassicae (light leaf spot) apothecia was studied on petiole debris from artificially infected oilseed rape leaves incubated at temperatures from 6 to 22 degreesC under different wetness regimes and in 16 h light/8 h dark or continuous darkness. There was no significant difference between light treatments in numbers of apothecia that developed. Mature apothecia developed at temperatures from 5 to 18 degreesC but not at 22 degreesC. The rate of apothecial development decreased as temperature decreased from 18 to 5 degreesC; mature apothecia were first observed after 5 days at 18 degreesC and after 15 days at 6 degreesC. Models were fitted to estimates of the time (days) for 50% of the maximum number of apothecia to develop (t(1); model 1, t(1) = 7.6 + 55.8(0.839)(T)) and the time for 50% of the maximum number of apothecia to decay (t(2); model 2, t(2) = 24.2 + 387(0.730)(T)) at temperatures (T) from 6 to 18 degreesC. An interruption in wetness of the petiole debris for 4 days after 4, 7, or 10 days of wetness delayed the time to observation of the first mature apothecia for approximate to4 days and decreased the number of apothecia produced (by comparison with continuous wetness). A relationship was found between water content of pod debris and electrical resistance measured by a debris-wetness sensor. The differences between values of tl predicted by model 1 and observed values of t(1) were 1 to 9 days. Model 2 did not predict t(2); apothecia decayed more quickly under natural conditions than predicted by model 2.Peer reviewe

Production of human recombinant proapolipoprotein A-I in Escherichia coli: purification and biochemical characterization

A human liver cDNA library was used to isolate a clone coding for apolipoprotein A-I (Apo A-I). The clone carries the sequence for the prepeptide (18 amino acids), the propeptide (6 amino acids), and the mature protein (243 amino acids). A coding cassette for the proapo A-I molecule was reconstructed by fusing synthetic sequences, chosen to optimize expression and specifying the amino-terminal methionine and amino acids -6 to +14, to a large fragment of the cDNA coding for amino acids 15-243. The module was expressed in pOTS-Nco, an Escherichia coli expression vector carrying the regulatable X P^ promoter, leading to the production of proapolipoprotein A-I at up to 10% of total soluble proteins. The recombinant polypeptide was purified and characterized in terms of apparent molecular mass, isoelectric point, and by both chemical and enzymatic peptide mapping. In addition, it was assayed in vitro for the stimulation of the enzyme lecithin: cholesterol acyltransferase. The data show for the first time that proapo A-I can be produced efficiently in E. coli as a stable and undegraded protein having physical and functional properties indistinguishable from those of the natural product

Remarks on the tight-binding model of graphene

We address a simple but fundamental issue arising in the study of graphene, as well as of other systems that have a crystalline structure with more than one atom per unit cell. For these systems, the choice of the tight-binding basis is not unique. For monolayer graphene two bases are widely used in the literature. While the expectation values of operators describing physical quantities should be independent of basis, the form of the operators may depend on the basis, especially in the presence of disorder or of an applied magnetic field. Using the inappropriate form of certain operators may lead to erroneous physical predictions. We discuss the two bases used to describe monolayer graphene, as well as the form of the most commonly used operators in the two bases. We repeat our analysis for the case of bilayer graphene.Comment: 15 pages, 4 figure

Mode coupling control in a resonant device: application to solid-state ring lasers

A theoretical and experimental investigation of the effects of mode coupling in a resonant macro- scopic quantum device is achieved in the case of a ring laser. In particular, we show both analytically and experimentally that such a device can be used as a rotation sensor provided the effects of mode coupling are controlled, for example through the use of an additional coupling. A possible general- ization of this example to the case of another resonant macroscopic quantum device is discussed

DYNAMO-MAS: a multi-agent system for ontology evolution from text

International audienceManual ontology development and evolution are complex and time-consuming tasks, even when textual documents are used as knowledge sources in addition to human expertise or existing ontologies. Processing natural language in text produces huge amounts of linguistic data that need to be filtered out and structured. To support both of these tasks, we have developed DYNAMO-MAS, an interactive tool based on an adaptive multi-agent system (adaptive MAS or AMAS) that builds and evolves ontologies from text. DYNA-MO-MAS is a partner system to build ontologies; the ontologist interacts with the system to validate or modify its outputs. This paper presents the architecture of DYNAMO-MAS, its operating principles and its evaluation on three case studies

Scattering theory on graphs

We consider the scattering theory for the Schr\"odinger operator -\Dc_x^2+V(x) on graphs made of one-dimensional wires connected to external leads. We derive two expressions for the scattering matrix on arbitrary graphs. One involves matrices that couple arcs (oriented bonds), the other involves matrices that couple vertices. We discuss a simple way to tune the coupling between the graph and the leads. The efficiency of the formalism is demonstrated on a few known examples.Comment: 21 pages, LaTeX, 10 eps figure

Limitations of variable number of tandem repeat typing identified through whole genome sequencing of Mycobacterium avium subsp. paratuberculosis on a national and herd level

Background: Mycobacterium avium subsp. paratuberculosis (MAP), the causative bacterium of Johne’s disease in dairy cattle, is widespread in the Canadian dairy industry and has significant economic and animal welfare implications. An understanding of the population dynamics of MAP can be used to identify introduction events, improve control efforts and target transmission pathways, although this requires an adequate understanding of MAP diversity and distribution between herds and across the country. Whole genome sequencing (WGS) offers a detailed assessment of the SNP-level diversity and genetic relationship of isolates, whereas several molecular typing techniques used to investigate the molecular epidemiology of MAP, such as variable number of tandem repeat (VNTR) typing, target relatively unstable repetitive elements in the genome that may be too unpredictable to draw accurate conclusions. The objective of this study was to evaluate the diversity of bovine MAP isolates in Canadian dairy herds using WGS and then determine if VNTR typing can distinguish truly related and unrelated isolates.<p></p> Results: Phylogenetic analysis based on 3,039 SNPs identified through WGS of 124 MAP isolates identified eight genetically distinct subtypes in dairy herds from seven Canadian provinces, with the dominant type including over 80% of MAP isolates. VNTR typing of 527 MAP isolates identified 12 types, including “bison type” isolates, from seven different herds. At a national level, MAP isolates differed from each other by 1–2 to 239–240 SNPs, regardless of whether they belonged to the same or different VNTR types. A herd-level analysis of MAP isolates demonstrated that VNTR typing may both over-estimate and under-estimate the relatedness of MAP isolates found within a single herd.<p></p> Conclusions: The presence of multiple MAP subtypes in Canada suggests multiple introductions into the country including what has now become one dominant type, an important finding for Johne’s disease control. VNTR typing often failed to identify closely and distantly related isolates, limiting the applicability of using this typing scheme to study the molecular epidemiology of MAP at a national and herd-level.<p></p&gt

Quantum oscillations and decoherence due to electron-electron interaction in metallic networks and hollow cylinders

We have studied the quantum oscillations of the conductance for arrays of connected mesoscopic metallic rings, in the presence of an external magnetic field. Several geometries have been considered: a linear array of rings connected with short or long wires compared to the phase coherence length, square networks and hollow cylinders. Compared to the well-known case of the isolated ring, we show that for connected rings, the winding of the Brownian trajectories around the rings is modified, leading to a different harmonics content of the quantum oscillations. We relate this harmonics content to the distribution of winding numbers. We consider the limits where coherence length $L_\varphi$ is small or large compared to the perimeter $L$ of each ring constituting the network. In the latter case, the coherent diffusive trajectories explore a region larger than $L$, whence a network dependent harmonics content. Our analysis is based on the calculation of the spectral determinant of the diffusion equation for which we have a simple expression on any network. It is also based on the hypothesis that the time dependence of the dephasing between diffusive trajectories can be described by an exponential decay with a single characteristic time $\tau_\varphi$ (model A) . At low temperature, decoherence is limited by electron-electron interaction, and can be modelled in a one-electron picture by the fluctuating electric field created by other electrons (model B). It is described by a functional of the trajectories and thus the dependence on geometry is crucial. Expressions for the magnetoconductance oscillations are derived within this model and compared to the results of model A. It is shown that they involve several temperature-dependent length scales.Comment: 35 pages, revtex4, 25 figures (34 pdf files

High-contrast 10-fs OPCPA-based Front-End for the Apollon-10PW laser (Orale)

International audienceWe present a high-contrast 10-fs Front-End for Ti:sapphire PW-lasers within the Apollon-10PW project. This injector uses OPCPA pumped at 100 Hz by Yb-based CPA chain. Combination of OPCPA and XPW permits a >10 12 contrast ratio

Rewriting Logic Semantics of a Plan Execution Language

The Plan Execution Interchange Language (PLEXIL) is a synchronous language developed by NASA to support autonomous spacecraft operations. In this paper, we propose a rewriting logic semantics of PLEXIL in Maude, a high-performance logical engine. The rewriting logic semantics is by itself a formal interpreter of the language and can be used as a semantic benchmark for the implementation of PLEXIL executives. The implementation in Maude has the additional benefit of making available to PLEXIL designers and developers all the formal analysis and verification tools provided by Maude. The formalization of the PLEXIL semantics in rewriting logic poses an interesting challenge due to the synchronous nature of the language and the prioritized rules defining its semantics. To overcome this difficulty, we propose a general procedure for simulating synchronous set relations in rewriting logic that is sound and, for deterministic relations, complete. We also report on two issues at the design level of the original PLEXIL semantics that were identified with the help of the executable specification in Maude