Tracking system study

A digital computer program was generated which mathematically describes an optimal estimator-controller technique as applied to the control of antenna tracking systems used by NASA. Simulation studies utilizing this program were conducted using the IBM 360/91 computer. The basic ideas of applying optimal estimator-controller techniques to antenna tracking systems are discussed. A survey of existing tracking methods is given along with shortcomings and inherent errors. It is explained how these errors can be considerably reduced if optimal estimation and control are used. The modified programs generated in this project are described and the simulation results are summarized. The new algorithms for direct synthesis and stabilization of the systems including nonlinearities, are presented

Opposing effects of final population density and stress on Escherichia coli mutation rate

Evolution depends on mutations. For an individual genotype, the rate at which mutations arise is known to increase with various stressors (stress-induced mutagenesis-SIM) and decrease at high final population density (density-associated mutation-rate plasticity-DAMP). We hypothesised that these two forms of mutation-rate plasticity would have opposing effects across a nutrient gradient. Here we test this hypothesis, culturing Escherichia coli in increasingly rich media. We distinguish an increase in mutation rate with added nutrients through SIM (dependent on error-prone polymerases Pol IV and Pol V) and an opposing effect of DAMP (dependent on MutT, which removes oxidised G nucleotides). The combination of DAMP and SIM results in a mutation rate minimum at intermediate nutrient levels (which can support 7 × 10  cells ml ). These findings demonstrate a strikingly close and nuanced relationship of ecological factors-stress and population density-with mutation, the fuel of all evolution

Aminostratigraphy and Oxygen Isotope Stratigraphy of Marine Terrace Deposits Palos Verdes Hills and San Pedro Areas Los Angeles County California

Amino acid and oxygen isotope data for fossils from terraces of the Palos Verdes Hills and San Pedro areas in Los Angeles County California shed new light on the ages of terraces sea level history marine paleotemperatures and late Quaternary tectonics in this region Low terraces on the Palos Verdes peninsula correlate with the 80 ka and 125 ka sea level highstands that are also recorded as terraces on other coasts In San Pedro the Palos Verdes sand the deposit on what is mapped as the first terrace by Woodring and others 1946 was previously thought to be a single deposit amino acid oxygen isotope V series and faunal data indicate that deposits of two ages representing the 80 ka and l25 ka high stands occur within this unit Oxygen isotope data show that on open exposed parts of the Palos Verdes peninsula ocean waters during the l25 ka highstand were cooler than present by about 2 3 2 60C similar to what has been reported for other exposed coastal areas in California In contrast in the protected embayment environment around San Pedro water temperatures during the 125 ka highstand were as warm or warmerthan present During the 80 ka highstand water temperatures were significantly cooler than present even in the relatively protected embayment environment of the San Pedro area Late Quaternary tectonic uplift rates can be calculated from terrace ages and elevations Correlation of the lowest terraces around the Point Fermin area shows that the Cabrillo fault has a late Quaternary vertical movement rate of 0 20 m ka based on the difference in uplift rates on the upthrown and downthrown sides of the fault Elsewhere in the Palos Verdes Hills San Pedro area late Quaternary uplift rates vary from 0 32 m ka to possibly as high as 0 72 m ka These rates which reflect vertical movement on the Palos Verdes fault are in broad agreement with estimated Holocene vertical rates of movement determined for offshore portions of the faul

Vortex lattice stability and phase coherence in three-dimensional rapidly rotating Bose condensates

We establish the general equations of motion for the modes of a vortex lattice in a rapidly rotating Bose-Einstein condensate in three dimensions, taking into account the elastic energy of the lattice and the vortex line bending energy. As in two dimensions, the vortex lattice supports Tkachenko and gapped sound modes. In contrast, in three dimensions the Tkachenko mode frequency at long wavelengths becomes linear in the wavevector for any propagation direction out of the transverse plane. We compute the correlation functions of the vortex displacements and the superfluid order parameter for a homogeneous Bose gas of bounded extent in the axial direction. At zero temperature the vortex displacement correlations are convergent at large separation, but at finite temperatures, they grow with separation. The growth of the vortex displacements should lead to observable melting of vortex lattices at higher temperatures and somewhat lower particle number and faster rotation than in current experiments. At zero temperature a system of large extent in the axial direction maintains long range order-parameter correlations for large separation, but at finite temperatures the correlations decay with separation.Comment: 10 pages, 2 figures, Changes include the addition of the particle density - vortex density coupling and the correct value of the shear modulu

Spontaneous mutation rate is a plastic trait associated with population density across domains of life

Rates of random, spontaneous mutation can vary plastically, dependent upon the environment. Such plasticity affects evolutionary trajectories and may be adaptive. We recently identified an inverse plastic association between mutation rate and population density at 1 locus in 1 species of bacterium. It is unknown how widespread this association is, whether it varies among organisms, and what molecular mechanisms of mutagenesis or repair are required for this mutation-rate plasticity. Here, we address all 3 questions. We identify a strong negative association between mutation rate and population density across 70 years of published literature, comprising hundreds of mutation rates estimated using phenotypic markers of mutation (fluctuation tests) from all domains of life and viruses. We test this relationship experimentally, determining that there is indeed density-associated mutation-rate plasticity (DAMP) at multiple loci in both eukaryotes and bacteria, with up to 23-fold lower mutation rates at higher population densities. We find that the degree of plasticity varies, even among closely related organisms. Nonetheless, in each domain tested, DAMP requires proteins scavenging the mutagenic oxidised nucleotide 8-oxo-dGTP. This implies that phenotypic markers give a more precise view of mutation rate than previously believed: having accounted for other known factors affecting mutation rate, controlling for population density can reduce variation in mutation-rate estimates by 93%. Widespread DAMP, which we manipulate genetically in disparate organisms, also provides a novel trait to use in the fight against the evolution of antimicrobial resistance. Such a prevalent environmental association and conserved mechanism suggest that mutation has varied plastically with population density since the early origins of life

Human skeletal muscle feed arteries: evidence of regulatory potential

Aim Recently, it has been recognized that human skeletal muscle feed arteries can be harvested during exploratory surgery for melanoma. This approach provides vessels for in vitro study from a wide spectrum of relatively healthy humans. Although, the regulatory role of skeletal muscle feed arteries in rodent models has been documented, whether such vessels in humans possess this functionality is unknown. Methods Therefore, skeletal muscle feed arteries (~950 μm OD) from 10 humans (48 ± 4, 27–64 years) were studied using pressure myography. Vessel function was assessed using potassium chloride (KCl), phenylephrine (PE), acetylcholine (ACh) and sodium nitroprusside (SNP) concentration–response curves (CRCs) to characterize non-receptor and receptor-mediated vasoconstriction as well as endothelium-dependent and independent vasodilation respectively. To understand the physiological relevance of the diameter changes as a result of pharmacological stimulation, the estimated conductance ratio (CR) was calculated. Results Vessel function protocols revealed significant vasoconstriction in response to PE and KCl (35 ± 6; 43 ± 9%vasoconstriction, respectively) and significant vasodilation with ACh and SNP (85 ± 7; 121 ± 17% vasodilation, respectively). Both PE and KCl significantly reduced the CR (0.26 ± 0.05 and 0.23 ± 0.07, respectively), whereas ACh and SNP increased the CR (2.56 ± 0.10 and 5.32 ± 1.3, respectively). Conclusion These novel findings provide evidence that human skeletal muscle feed arteries are capable of generating significant diameter changes that would translate into significant changes in vascular conductance. Thus, human skeletal muscle feed arteries likely play a significant role in regulating vascular conductance and subsequently blood flow in vivo

α1-Adrenergic responsiveness in human skeletal muscle feed arteries: the impact of reducing extracellular pH

What is the central question of this study? In human arteries involved in the regulation of muscle blood flow, there is a lack of data about whether acidosis alters vascular sensitivity to vasoactive agents, as well as altering endothelium dependent vasorelaxation. Little is known about the interaction of metabolites and vascular function in human skeletal muscle feed arteries. • What is the main finding and its importance? Increasing acidosis attenuated the response and sensitivity of the arteries to phenylephrine; this effect was selective to the receptor over smooth muscle. Acidosis did not alter endothelium dependent vasorelaxation. Impaired vasoconstriction coupled with intact vasorelaxation, promotes decreased vascular tone with exposure to acidosis, and may contribute to sympatholysis during exercise

Estimation of the rigid-body motion from three-dimensional images using a generalized center-of-mass points approach

We present an analytical method for the estimation of rigid-body motion in sets of three-dimensional (3-D) SPECT and PET slices. This method utilizes mathematically defined generalized center-of-mass points in images, requiring no segmentation. It can be applied to compensation of the rigid-body motion in both SPECT and PET, once a series of 3-D tomographic images are available. We generalized the formula for the center-of-mass to obtain a family of points comoving with the object\u27s rigid-body motion. From the family of possible points we chose the best three points which resulted in the minimum root-mean-square difference between images as the generalized center-of-mass points for use in estimating motion. The estimated motion was used to sum the sets of tomographic images, or incorporated in the iterative reconstruction to correct for motion during reconstruction of the combined projection data. For comparison, the principle-axes method was also applied to estimate the rigid-body motion from the same tomographic images. To evaluate our method for different noise levels, we performed simulations with the MCAT phantom. We observed that though noise degraded the motion-detection accuracy, our method helped in reducing the motion artifact both visually and quantitatively. We also acquired four sets of the emission and transmission data of the Data Spectrum Anthropomorphic Phantom positioned at four different locations and/or orientations. From these we generated a composite acquisition simulating periodic phantom movements during acquisition. The simulated motion was calculated from the generalized center-of-mass points calculated from the tomographic images reconstructed from individual acquisitions. We determined that motion-compensation greatly reduced the motion artifact. Finally, in a simulation with the gated MCAT phantom, an exaggerated rigid-body motion was applied to the end-systolic frame. The motion was estimated from the end-diastolic and end-systolic images, and used to sum them into a summed image without obvious artifact. Compared to the principle-axes method, in two of the three comparisons with anthropomorphic phantom data our method estimated the motion in closer agreement to the Polaris system than the principal-axes method, while the principle-axes method gave a more accurate estimation of motion in most cases for the MCAT simulations. As an image-driven approach, our method assumes angularly com plete data sets for each state of motion. We expert this method to be applied in correction of respiratory motion in respiratory gated SPECT, and respiratory or other rigid-body motion in PET. © 2006 IEEE