A guide to the removal of heavy silicone oil

Aim: Heavy silicone oil removal can be challenging and differs considerably from conventional oil. Traditionally, strong active aspiration had to be applied through a long 18G needle just above the optic disc. We present a novel technique using a much shorter (7.5 mm) and smaller (20G) needle allowing its removal "from a distance." Method: Active aspiration on a vacuum of 600 mm Hg of the "viscous fluid injector" was applied using the 20G cannula in a polymethylmethacrylate model eye chamber that was surface-modified to mimic the surface properties of the retina. Measurements were taken using still photographs. Results: Under injection the maximum diameter of a silicone oil bubble supported by interfacial tension alone was 5 mm for a steel and 7 mm for a polyurethane cannula. Under suction, the silicone bubble changed shape and became conical, thus further increasing the cannula's reach. This conical shape illustrated "tubeless siphoning," which is a physical property of non-Newtonian fluids. Discussion: The use of shorter and smaller gauge cannula for removal of Densiron obviates the need to enlarge the sclerotomy beyond 20G or to apply suction in close proximity to disc and fovea. This potentially reduces the risk of iatrogenic damage such as entry site tears or postoperative hypotony.published_or_final_versio

Peel and peel again

Aim: To determine if the internal limiting membrane (ILM) was present in the epiretinal membrane (ERM) when we deliberately tried to perform a "double peel" for macular pucker. Methods: Pars-plana vitrectomy and a "double peel" were carried out. The ERM and ILM were stained with Trypan Blue and peeled separately over the same area. The amount of ERM present in ILM specimens and the amount of ILM present in ERM specimens were evaluated by histological examination. Results: Seventeen eyes in 17 patients were included. It was possible to double peel in all cases. Five of 17 ERM specimens (29%) contained ILM fragments. When ILM was present on the ERM, it represented less than 50% of the sample. One ILM specimen was lost as result of an administrative error; of the remaining 16 specimens, residual ERM was found in six, and cellular remnants were observed on the vitreous surface in a further six of the ILMs. Clinically, no recurrence of ERM was found. Conclusion: ILM was present in some ERM specimens seemingly over the same area that an intact ILM was subsequently peel. We speculate that the ILM in the ERM represent a secondary basement membrane and that the surgical plane of dissection for most ERM peel is between the ERM and the native ILM, making it feasible to double peel routinely.published_or_final_versio

Genome-wide associations for birth weight and correlations with adult disease

Birth weight (BW) has been shown to be influenced by both fetal and maternal factors and in observational studies is reproducibly associated with future risk of adult metabolic diseases including type 2 diabetes (T2D) and cardiovascular disease. These life-course associations have often been attributed to the impact of an adverse early life environment. Here, we performed a multi-ancestry genome-wide association study (GWAS) meta-analysis of BW in 153,781 individuals, identifying 60 loci where fetal genotype was associated with BW ($\textit{P}$  < 5 × 10$^{-8}$). Overall, approximately 15% of variance in BW was captured by assays of fetal genetic variation. Using genetic association alone, we found strong inverse genetic correlations between BW and systolic blood pressure ($\textit{R}$ $_{g}$ = -0.22, $\textit{P}$  = 5.5 × 10$^{-13}$), T2D ($\textit{R}$ $_{g}$ = -0.27, $\textit{P}$  = 1.1 × 10$^{-6}$) and coronary artery disease ($\textit{R}$ $_{g}$ = -0.30, $\textit{P}$  = 6.5 × 10$^{-9}$). In addition, using large -cohort datasets, we demonstrated that genetic factors were the major contributor to the negative covariance between BW and future cardiometabolic risk. Pathway analyses indicated that the protein products of genes within BW-associated regions were enriched for diverse processes including insulin signalling, glucose homeostasis, glycogen biosynthesis and chromatin remodelling. There was also enrichment of associations with BW in known imprinted regions ($\textit{P}$ = 1.9 × 10$^{-4}$). We demonstrate that life-course associations between early growth phenotypes and adult cardiometabolic disease are in part the result of shared genetic effects and identify some of the pathways through which these causal genetic effects are mediated.For a full list of the funders pelase visit the publisher's website and look at the supplemetary material provided. Some of the funders are: British Heart Foundation, Cancer Research UK, Medical Research Council, National Institutes of Health, Royal Society and Wellcome Trust

Therapeutic Potential of HDL in Cardioprotection and Tissue Repair

Epidemiological studies support a strong association between high-density lipoprotein (HDL) cholesterol levels and heart failure incidence. Experimental evidence from different angles supports the view that low HDL is unlikely an innocent bystander in the development of heart failure. HDL exerts direct cardioprotective effects, which are mediated via its interactions with the myocardium and more specifically with cardiomyocytes. HDL may improve cardiac function in several ways. Firstly, HDL may protect the heart against ischaemia/reperfusion injury resulting in a reduction of infarct size and thus in myocardial salvage. Secondly, HDL can improve cardiac function in the absence of ischaemic heart disease as illustrated by beneficial effects conferred by these lipoproteins in diabetic cardiomyopathy. Thirdly, HDL may improve cardiac function by reducing infarct expansion and by attenuating ventricular remodelling post-myocardial infarction. These different mechanisms are substantiated by in vitro, ex vivo, and in vivo intervention studies that applied treatment with native HDL, treatment with reconstituted HDL, or human apo A-I gene transfer. The effect of human apo A-I gene transfer on infarct expansion and ventricular remodelling post-myocardial infarction illustrates the beneficial effects of HDL on tissue repair. The role of HDL in tissue repair is further underpinned by the potent effects of these lipoproteins on endothelial progenitor cell number, function, and incorporation, which may in particular be relevant under conditions of high endothelial cell turnover. Furthermore, topical HDL therapy enhances cutaneous wound healing in different models. In conclusion, the development of HDL-targeted interventions in these strategically chosen therapeutic areas is supported by a strong clinical rationale and significant preclinical data.status: publishe

Surgical management of massive sub-macular hemorrhage associated with age-related macular degeneration

Purpose: To investigate the safety and efficacy of surgical drainage of massive and elevated submacular hemorrhage associated with age-related macular degeneration through circumferential peripheral 180° temporal retinotomy. Methods: Prospective interventional case series of first eyes of six consecutive patients with large elevated submacular hemorrhage. The surgical approach consisted of combined cataract surgery, three-port pars plana vitrectomy, induction of retinal detachment, and circumferential peripheral temporal 180° retinotomy. The temporal retina was reflected and the solid blood clot was removed with a vitreous cutter along with the choroidal neovascular complex. Silicone oil was used as an internal temponade in all patients. Complete clinical examination was performed in all cases preoperatively and postoperatively at 1 and 3 months. After removal of silicone oil, the patients were observed for 12 months. Results: Submacular hemorrhage was completely removed in all cases the next day after the surgery. All patients experienced improvement of central scotoma. Twelve months after silicone oil removal, visual acuity was defined as improvement in 5 eyes (83%) and stable in 1 eye (17%). No recurrence or complications were observed during the period of follow-up. Conclusion: Our surgical approach could be efficacious to remove a large elevated submacular hemorrhage that is not feasible for any other treatment. It may help to improve the central scotoma and results in limited visual improvement in selected patients

Treatment of exposed explants with donor sclera and amniotic membrane [3]

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Perfluorocarbon liquid assisted large retinal epithelium patching in sub-macular hemorrhage secondary to age related macular degeneration

Background: We investigate the safety and feasibility of large retinal pigmentary epithelium (RPE)-Choroid-free graft after surgical drainage of massive sub-macular hemorrhage (SMH) due to age-related macular degeneration (ARMD). Methods: Four previously untreated patients (three females and one male) underwent to three port pars plana vitrectomy, induction of retinal detachment and peripheral temporal 180° retinotomy. The retina was then folded nasally, to allow access for removal of sub-macular Hg and CNV complex. A full-thickness-large autologus Chorio-RPE patch was grafted. Silicone oil was used as endotemponade for approximately 12 weeks. After removal of silicone oil, the patients were followed-up for 6 months. Results: SMH was completely removed in all cases. It was possible to graft a large RPE patch safely that is sufficiently large to cover the entire defect of macular RPE. At last follow-up, improvement in visual acuity (from 3± 0.9 to 55± 9 ETDRS letters) and recovery of central fixation was observed in all patients. Conclusions: Our surgical technique for large elevated SMH seems to be feasible and efficacious approach to harvest and relocate large RPE patch and to save limited vision in selected patients. © Springer-Verlag 2008.link_to_subscribed_fulltex

Surgical management of massive sub-macular hemorrhage associated with age-related macular degeneration

Purpose: To investigate the safety and efficacy of surgical drainage of massive and elevated submacular hemorrhage associated with age-related macular degeneration through circumferential peripheral 180° temporal retinotomy. Methods: Prospective interventional case series of first eyes of six consecutive patients with large elevated submacular hemorrhage. The surgical approach consisted of combined cataract surgery, three-port pars plana vitrectomy, induction of retinal detachment, and circumferential peripheral temporal 180° retinotomy. The temporal retina was reflected and the solid blood clot was removed with a vitreous cutter along with the choroidal neovascular complex. Silicone oil was used as an internal temponade in all patients. Complete clinical examination was performed in all cases preoperatively and postoperatively at 1 and 3 months. After removal of silicone oil, the patients were observed for 12 months. Results: Submacular hemorrhage was completely removed in all cases the next day after the surgery. All patients experienced improvement of central scotoma. Twelve months after silicone oil removal, visual acuity was defined as improvement in 5 eyes (83%) and stable in 1 eye (17%). No recurrence or complications were observed during the period of follow-up. Conclusion: Our surgical approach could be efficacious to remove a large elevated submacular hemorrhage that is not feasible for any other treatment. It may help to improve the central scotoma and results in limited visual improvement in selected patients