TTF-1/p63-positive poorly differentiated NSCLC: A histogenetic hypothesis from the basal reserve cell of the terminal respiratory unit

TTF-1 is expressed in the alveolar epithelium and in the basal cells of distal terminal bronchioles. It is considered the most sensitive and specific marker to define the adenocarcinoma arising from the terminal respiratory unit (TRU). TTF-1, CK7, CK5/6, p63 and p40 are useful for typifying the majority of non-small-cell lung cancers, with TTF and CK7 being typically expressed in adenocarcinomas and the latter three being expressed in squamous cell carcinoma. As tumors with coexpression of both TTF-1 and p63 in the same cells are rare, we describe different cases that coexpress them, suggesting a histogenetic hypothesis of their origin. We report 10 cases of poorly differentiated non-small-cell lung carcinoma (PD-NSCLC). Immunohistochemistry was performed by using TTF-1, p63, p40 (∆Np63), CK5/6 and CK7. EGFR and BRAF gene mutational analysis was performed by using real-time PCR. All the cases showed coexpression of p63 and TTF-1. Six of them showing CK7+ and CK5/6− immunostaining were diagnosed as “TTF-1+ p63+ adenocarcinoma”. The other cases of PD-NSCLC, despite the positivity for CK5/6, were diagnosed as “adenocarcinoma, solid variant”, in keeping with the presence of TTF-1 expression and p40 negativity. A “wild type” genotype of EGFR was evidenced in all cases. TTF1 stained positively the alveolar epithelium and the basal reserve cells of TRU, with the latter also being positive for p63. The coexpression of p63 and TTF-1 could suggest the origin from the basal reserve cells of TRU and represent the capability to differentiate towards different histogenetic lines. More aggressive clinical and morphological features could characterize these “basal-type tumors” like those in the better known “basal-like” cancer of the breast

Development in Web-based Laboratory Sessions

The laboratory experiments can help the undergraduate students to gain a better understanding the theoretical problem. This paper proposes an educational tool made up a user-friendly interface controlling experimental boards and instrumentation device with a new approach based on FieldPointTM module and the web publishing tool of the LabVIEW. The proposed system allows students to improve their knowledge in the field of optical sensing devices, virtual instrumentation, data acquisition systems, and signal processing

Orchestrating Lightpath Adaptation and Flexible Functional Split to Recover Virtualized RAN Connectivity

This study shows that a two-step recovery scheme orchestrating lightpath transmission adaptation and evolved NodeB (eNB) functional split reconfiguration preserves the Virtualized RAN fronthaul connectivity even when network capacity is scarce.This work has been partially funded by the EU H2020 “5G-Transformer” Project (grant no. 761536

Orchestrating Lightpath Recovery and Flexible Functional Split to Preserve Virtualized RAN Connectivity

In the next-generation radio access network (NG RAN), the next-generation evolved NodeBs (gNBs) will be, likely, split into virtualized central units (CUs) and distributed units (DUs) interconnected by a fronthaul network. Because of fronthaul latency and capacity requirements, optical metro-ring networks are among the main candidates for supporting converged 5G and non-5G services. In this scenario, a degradation in the quality of transmission of the lightpaths connecting DU and CU can be revealed (or anticipated) based on monitoring techniques. Thus, the lightpath transmission parameters can be adapted to maintain the required bit error rate (BER). However, in specific cases, the original requested capacity between DU and CU could be not guaranteed, thus impacting the service. In this case, another DU–CU connectivity should be considered, relying on a change of the so-called functional split. This study proposes a two-step recovery scheme orchestrating lightpath transmission adaptation and functional split reconfiguration to guarantee the requested connectivity in a virtualized RAN fronthaul. Results show that, for the connections that cannot be transported by the original lightpath, a graceful degradation followed by a recovery is possible within tens of seconds.This work was partly funded by the project H2020-ICT-2016-2 “5G-TRANSFORMER” (761536

Polyethylene imine-based receptor immobilization for label free bioassays

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor

Transcriptomic and proteomic insights into innate immunity and adaptations to a symbiotic lifestyle in the gutless marine worm Olavius algarvensis

Background: The gutless marine worm Olavius algarvensis has a completely reduced digestive and excretory system, and lives in an obligate nutritional symbiosis with bacterial symbionts. While considerable knowledge has been gained of the symbionts, the host has remained largely unstudied. Here, we generated transcriptomes and proteomes of O. algarvensis to better understand how this annelid worm gains nutrition from its symbionts, how it adapted physiologically to a symbiotic lifestyle, and how its innate immune system recognizes and responds to its symbiotic microbiota. Results: Key adaptations to the symbiosis include (i) the expression of gut-specific digestive enzymes despite the absence of a gut, most likely for the digestion of symbionts in the host's epidermal cells; (ii) a modified hemoglobin that may bind hydrogen sulfide produced by two of the worm's symbionts; and (iii) the expression of a very abundant protein for oxygen storage, hemerythrin, that could provide oxygen to the symbionts and the host under anoxic conditions. Additionally, we identified a large repertoire of proteins involved in interactions between the worm's innate immune system and its symbiotic microbiota, such as peptidoglycan recognition proteins, lectins, fibrinogen-related proteins, Toll and scavenger receptors, and antimicrobial proteins. Conclusions: We show how this worm, over the course of evolutionary time, has modified widely-used proteins and changed their expression patterns in adaptation to its symbiotic lifestyle and describe expressed components of the innate immune system in a marine oligochaete. Our results provide further support for the recent realization that animals have evolved within the context of their associations with microbes and that their adaptive responses to symbiotic microbiota have led to biological innovations

The violent past of Cygnus X-2

Cygnus X-2 appears to be the descendant of an intermediate-mass X-ray binary (IMXB). Using Mazzitelli's (1989) stellar code we compute detailed evolutionary sequences for the system and find that its prehistory is sensitive to stellar input parameters, in particular the amount of core overshooting during the main-sequence phase. With standard assumptions for convective overshooting a case B mass transfer starting with a 3.5 M_sun donor star is the most likely evolutionary solution for Cygnus X-2. This makes the currently observed state rather short-lived, of order 3 Myr, and requires a formation rate > 1e-7 - 1e-6 per yr of such systems in the Galaxy. Our calculations show that neutron star IMXBs with initially more massive donors (> 4 M_sun) encounter a delayed dynamical instability; they are unlikely to survive this rapid mass transfer phase. We determine limits for the age and initial parameters of Cygnus X-2 and calculate possible dynamical orbits of the system in a realistic Galactic potential, given its observed radial velocity. We find trajectories which are consistent with a progenitor binary on a circular orbit in the Galactic plane inside the solar circle that received a kick velocity < 200 km/s at the birth of the neutron star. The simulations suggests that about 7% of IMXBs receiving an arbitrary kick velocity from a standard kick velocity spectrum would end up in an orbit similar to Cygnus X-2, while about 10% of them reach yet larger Galactocentric distances.Comment: 9 pages, 12 figures, accepted for publication in MNRA