The effect of queue size on the throughput, in group failure mode, for the loaded transport channel

The external data flow decreases the throughput of the transport connection. The indicator of this external load is the queue size in front of the protocol data. In this article, using a mathematical model in analytical and numerical forms, the relation between the throughput of the channel and the protocol parameters are presented including the queue size parameter. In this work the effect of the queue size on time-out duration has been shown, which is one of the important parameters and it's studied weakly in researches. Also, the relation between round-trip delay, the reliability of the transmission of the information segments with queue size are also shown

Non-Contact Inspection of Railhead via Laser-Generated Rayleigh Waves and an Enhanced Matching Pursuit to Assist Detection of Surface and Subsurface Defects

Laser ultrasonic technology can provide a non-contact, reliable and efficient inspection of train rails. However, the laser-generated signals measured at the railhead are usually contaminated with a high level of noise and unwanted wave components that complicate the identification of defect echoes in the signal. This study explores the possibility of combining laser ultrasonic technology (LUT) and an enhanced matching pursuit (MP) to achieve a fully non-contact inspection of the rail track. A completely non-contact laser-based inspection system was used to generate and sense Rayleigh waves to detect artificial surface horizontal, surface edge, subsurface horizontal and subsurface vertical defects created at railheads of different dimensions. MP was enhanced by developing two novel dictionaries, which include a finite element method (FEM) simulation dictionary and an experimental dictionary. The enhanced MP was used to analyze the experimentally obtained laser-generated Rayleigh wave signals. The results show that the enhanced MP is highly effective in detecting defects by suppressing noise, and, further, it could also overcome the deficiency in the low repeatability of the laser-generated signals. The comparative analysis of MP with both the FEM simulation and experimental dictionaries shows that the enhanced MP with the FEM simulation dictionary is highly efficient in both noise removal and defect detection from the experimental signals captured by a laser-generated ultrasonic inspection system. The major novelty contributed by this research work is the enhanced MP method with the developments of, first, an FEM simulation dictionary and, second, an experimental dictionary that is especially suited for Rayleigh wave signals. Third, the enhanced MP dictionaries are created to process the Rayleigh wave signals generated by laser excitation and received using a 3D laser scanner. Fourth, we introduce a pioneer application of such laser-generated Rayleigh waves for inspecting surface and subsurface detects occurring in train rails

Reflective, polarizing, and magnetically soft amorphous Fe/Si multilayer neutron optics with isotope-enriched 11B4C inducing atomically flat interfaces

The utilization of polarized neutrons is of great importance in scientific disciplines spanning materials science, physics, biology, and chemistry. Polarization analysis offers insights into otherwise unattainable sample information such as magnetic domains and structures, protein crystallography, composition, orientation, ion-diffusion mechanisms, and relative location of molecules in multicomponent biological systems. State-of-the-art multilayer polarizing neutron optics have limitations, particularly low specular reflectivity and polarization at higher scattering vectors/angles, and the requirement of high external magnetic fields to saturate the polarizer magnetization. Here, we show that by incorporating 11B4C into Fe/Si multilayers, amorphization and smooth interfaces can be achieved, yielding higher neutron reflectivity, less diffuse scattering and higher polarization. Magnetic coercivity is eliminated, and magnetic saturation can be reached at low external fields (>2 mT). This approach offers prospects for significant improvement in polarizing neutron optics, enabling; nonintrusive positioning of the polarizer, enhanced flux, increased data accuracy, and further polarizing/analyzing methods at neutron scattering facilities

Beaconing Approaches in Vehicular Ad Hoc Networks: A Survey

A Vehicular Ad hoc Network (VANET) is a type of wireless ad hoc network that facilitates ubiquitous connectivity between vehicles in the absence of fixed infrastructure. Beaconing approaches is an important research challenge in high mobility vehicular networks with enabling safety applications. In this article, we perform a survey and a comparative study of state-of-the-art adaptive beaconing approaches in VANET, that explores the main advantages and drawbacks behind their design. The survey part of the paper presents a review of existing adaptive beaconing approaches such as adaptive beacon transmission power, beacon rate adaptation, contention window size adjustment and Hybrid adaptation beaconing techniques. The comparative study of the paper compares the representatives of adaptive beaconing approaches in terms of their objective of study, summary of their study, the utilized simulator and the type of vehicular scenario. Finally, we discussed the open issues and research directions related to VANET adaptive beaconing approaches.Ghafoor, KZ.; Lloret, J.; Abu Bakar, K.; Sadiq, AS.; Ben Mussa, SA. (2013). Beaconing Approaches in Vehicular Ad Hoc Networks: A Survey. Wireless Personal Communications. 73(3):885-912. doi:10.1007/s11277-013-1222-9S885912733ITS-Standards (1996) Intelligent transportation systems, U.S. Department of Transportation, http://www.standards.its.dot.gov/about.aspCheng, L., Henty, B., Stancil, D., Bai, F., & Mudalige, P. (2005). Mobile vehicle-to-vehicle narrow-band channel measurement and characterization of the 5.9 Ghz dedicated short range communication (DSRC) frequency band. IEEE Transactions on Selected Areas in Communications, 25(8), 1501–1516.van Eenennaam, E., Wolterink, K., Karagiannis, G., & Heijenk, G. (2009). Exploring the solution space of beaconing in vanets. In Proceedings of the 2009 IEEE international vehicular networking conference, Tokyo (pp. 1–8).Torrent-Moreno, M. 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Acknowledgment-based broadcast protocol for reliable and efficient data dissemination in vehicular ad-hoc networks. IEEE Transactions on Mobile Computing, 11(1), 33–46.Torrent-Moreno, M., Santi, P., & Hartenstein, H. (2006). Distributed fair transmit power adjustment for vehicular ad hoc networks. In Proceedings of the 2007 IEEE international conference on sensor and ad hoc communications and networks, Reston, VA (pp. 479–488).Artimy, M. (2007). Local density estimation and dynamic transmission-range assignment in vehicular ad hoc networks. IEEE Transactions on Intelligent Transportation Systems, 8(3), 400–412.Caizzone, G., Giacomazzi, P., Musumeci, L., & Verticale, G. (2005). A power control algorithm with high channel availability for vehicular ad hoc networks. In Proceedings of the 2005 IEEE international conference on communications, Seoul (pp. 3171–3176).Torrent-Moreno, M., Santi, P., & Hartenstein, H. (2009). 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Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

The exopolysaccharide galactosaminogalactan (GAG) is an important virulence factor of the fungal pathogen Aspergillus fumigatus. Deletion of a gene encoding a putative deacetylase, Agd3, leads to defects in GAG deacetylation, biofilm formation, and virulence. Here, we show that Agd3 deacetylates GAG in a metal-dependent manner, and is the founding member of carbohydrate esterase family CE18. The active site is formed by four catalytic motifs that are essential for activity. The structure of Agd3 includes an elongated substrate-binding cleft formed by a carbohydrate binding module (CBM) that is the founding member of CBM family 87. Agd3 homologues are encoded in previously unidentified putative bacterial exopolysaccharide biosynthetic operons and in other fungal genomes. The exopolysaccharide galactosaminogalactan (GAG) is an important virulence factor of the fungal pathogen Aspergillus fumigatus. Here, the authors study an A. fumigatus enzyme that deacetylates GAG in a metal-dependent manner and constitutes a founding member of a new carbohydrate esterase family.Bio-organic Synthesi

An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics

BackgroundThe majority of in vitro studies of medically relevant biofilms involve the development of biofilm on an inanimate solid surface. However, infection in vivo consists of biofilm growth on, or suspended within, the semi-solid matrix of the tissue, whereby current models do not effectively simulate the nature of the in vivo environment. This paper describes development of an in vitro method for culturing wound associated microorganisms in a system that combines a semi-solid collagen gel matrix with continuous flow of simulated wound fluid. This enables culture of wound associated reproducible steady state biofilms under conditions that more closely simulate the dynamic wound environment. To demonstrate the use of this model the antimicrobial kinetics of ceftazidime, against both mature and developing Pseudomonas aeruginosa biofilms, was assessed. In addition, we have shown the potential application of this model system for investigating microbial metabolomics by employing selected ion flow tube mass spectrometry (SIFT-MS) to monitor ammonia and hydrogen cyanide production by Pseudomonas aeruginosa biofilms in real-time. ResultsThe collagen wound biofilm model facilitates growth of steady-state reproducible Pseudomonas aeruginosa biofilms under wound like conditions. A maximum biofilm density of 1010 cfu slide-1 was achieved by 30 hours of continuous culture and maintained throughout the remainder of the experiment. Treatment with ceftazidime at a clinically relevant dose resulted in a 1.2 – 1.6 log reduction in biofilm density at 72 hours compared to untreated controls. Treatment resulted in loss of complex biofilm architecture and morphological changes to bacterial cells, visualised using confocal microscopy. When monitoring the biofilms using SIFT-MS, ammonia and hydrogen cyanide levels peaked at 12 hours at 2273 ppb (±826.4) and 138 ppb (±49.1) respectively and were detectable throughout experimentation. ConclusionsThe collagen wound biofilm model has been developed to facilitate growth of reproducible biofilms under wound-like conditions. We have successfully used this method to: (1) evaluate antimicrobial efficacy and kinetics, clearly demonstrating the development of antimicrobial tolerance in biofilm cultures; (2) characterise volatile metabolite production by P. aeruginosa biofilms, demonstrating the potential use of this method in metabolomics studies

A population-based study of tumor gene expression and risk of breast cancer death among lymph node-negative patients

INTRODUCTION: The Oncotype DX assay was recently reported to predict risk for distant recurrence among a clinical trial population of tamoxifen-treated patients with lymph node-negative, estrogen receptor (ER)-positive breast cancer. To confirm and extend these findings, we evaluated the performance of this 21-gene assay among node-negative patients from a community hospital setting. METHODS: A case-control study was conducted among 4,964 Kaiser Permanente patients diagnosed with node-negative invasive breast cancer from 1985 to 1994 and not treated with adjuvant chemotherapy. Cases (n = 220) were patients who died from breast cancer. Controls (n = 570) were breast cancer patients who were individually matched to cases with respect to age, race, adjuvant tamoxifen, medical facility and diagnosis year, and were alive at the date of death of their matched case. Using an RT-PCR assay, archived tumor tissues were analyzed for expression levels of 16 cancer-related and five reference genes, and a summary risk score (the Recurrence Score) was calculated for each patient. Conditional logistic regression methods were used to estimate the association between risk of breast cancer death and Recurrence Score. RESULTS: After adjusting for tumor size and grade, the Recurrence Score was associated with risk of breast cancer death in ER-positive, tamoxifen-treated and -untreated patients (P = 0.003 and P = 0.03, respectively). At 10 years, the risks for breast cancer death in ER-positive, tamoxifen-treated patients were 2.8% (95% confidence interval [CI] 1.7–3.9%), 10.7% (95% CI 6.3–14.9%), and 15.5% (95% CI 7.6–22.8%) for those in the low, intermediate and high risk Recurrence Score groups, respectively. They were 6.2% (95% CI 4.5–7.9%), 17.8% (95% CI 11.8–23.3%), and 19.9% (95% CI 14.2–25.2%) for ER-positive patients not treated with tamoxifen. In both the tamoxifen-treated and -untreated groups, approximately 50% of patients had low risk Recurrence Score values. CONCLUSION: In this large, population-based study of lymph node-negative patients not treated with chemotherapy, the Recurrence Score was strongly associated with risk of breast cancer death among ER-positive, tamoxifen-treated and -untreated patients

CNS Penetration of Intrathecal-Lumbar Idursulfase in the Monkey, Dog and Mouse: Implications for Neurological Outcomes of Lysosomal Storage Disorder

A major challenge for the treatment of many central nervous system (CNS) disorders is the lack of convenient and effective methods for delivering biological agents to the brain. Mucopolysaccharidosis II (Hunter syndrome) is a rare inherited lysosomal storage disorder resulting from a deficiency of iduronate-2-sulfatase (I2S). I2S is a large, highly glycosylated enzyme. Intravenous administration is not likely to be an effective therapy for disease-related neurological outcomes that require enzyme access to the brain cells, in particular neurons and oligodendrocytes. We demonstrate that intracerebroventricular and lumbar intrathecal administration of recombinant I2S in dogs and nonhuman primates resulted in widespread enzyme distribution in the brain parenchyma, including remarkable deposition in the lysosomes of both neurons and oligodendrocytes. Lumbar intrathecal administration also resulted in enzyme delivery to the spinal cord, whereas little enzyme was detected there after intraventricular administration. Mucopolysaccharidosis II model is available in mice. Lumbar administration of recombinant I2S to enzyme deficient animals reduced the storage of glycosaminoglycans in both superficial and deep brain tissues, with concurrent morphological improvements. The observed patterns of enzyme transport from cerebrospinal fluid to the CNS tissues and the resultant biological activity (a) warrant further investigation of intrathecal delivery of I2S via lumbar catheter as an experimental treatment for the neurological symptoms of Hunter syndrome and (b) may have broader implications for CNS treatment with biopharmaceuticals