Effect of spawning induced starvation on nutrient loss in males of the annual spawner Atlantic halibut (Hippoglossus hippoglossus)

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Low Omega-3 Levels in the Diet Disturbs Intestinal Barrier and Transporting Functions of Atlantic Salmon Freshwater and Seawater Smolts

Due to a limited access to marine raw materials from capture fisheries, Atlantic salmon feeds are currently based on mainly plant ingredients (75%) while only 25% come from traditional marine ingredients including marine fish meal and fish oil. Thus, current feeds contain less of the essential omega-3 fatty acids. The aim of the study was to assess the impact of different omega-3 levels in fish feed on intestinal barrier and transporting functions of Atlantic salmon freshwater and seawater smolts. Atlantic salmon were fed three levels of omega-3 (2, 1 and 0.5%) and fish performance was followed through smoltification and the subsequent seawater acclimation. Intestinal barrier and transporting functions were assessed using Ussing chamber methodology and combined with transcript analysis of tight junction related proteins and ion transporters. A linear decrease in growth was observed with decreasing omega-3 levels. Low (0.5%) inclusion of omega-3 impaired the barrier function of the proximal intestine compared to 2% inclusion. Further, low levels of omega-3 decrease the transepithelial electrical potential across the epithelium indicating disturbed ion transport. It can be concluded that low dietary levels of omega-3 impair somatic growth and intestinal function of Atlantic salmon.publishedVersio

Complete regression and systemic protective immune responses obtained in B16 melanomas after treatment with LTX-315

A manuscript version of this article is part of Ketil André Camilio's doctoral thesis, which is available in Munin at http://hdl.handle.net/10037/5489</a

Dietary Level of the Omega-3 Fatty Acids EPA and DHA Influence the Flesh Pigmentation in Atlantic Salmon

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Welfare and performance of ballan wrasse (Labrus bergylta) reared at two different temperatures after a preparatory feeding trial with enhanced dietary eicosapentaenoic acid

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Optimization of the Balance between Protein, Lipid and Carbohydrate in Diets for Lumpfish (Cyclopterus lumpus)

The aim of this study was to investigate how the dietary balance between protein, lipid, and carbohydrate affects growth, welfare, and health with focus on immune responses, in lumpfish of body weight ranges 1.7-10 g and 15-50 g. A three-component mixture design, with simultaneous variation of the three macronutrients was applied. Growth, tissue and plasma nutrient composition, welfare, cataract frequency, and immune responses in leukocytes isolated from the fish head kidney were studied. Most responses were linear and driven by dietary lipid level, with minor effects of carbohydrate and protein. The growth in 1.7-10 g fish followed a special cubic model with no clear optimum. The fastest growth in the 15-50 g fish was obtained with the diet containing 55% protein, 17% fat, and 6% carbohydrate. The high dietary levels of lipid also gave high lipid levels in the tissues and a trend of improved welfare score, but there were no effects on cataract. The diets with minimum and maximum protein gave suboptimal immune responses in isolated head kidney cells of the 15-50 g fish, while the fastest growing fish showed normal immune responses. We propose that diets for lumpfish from 10-50 g body weight should contain approximately 55% protein, minimum 10% lipid, and maximum 10% carbohydrate. These diets will also be suitable for lumpfish of 1.7-10 g.publishedVersio

The marine natural product mimic MPM-1 is cytolytic and induces DAMP release from human cancer cell lines

Bioprospecting contributes to the discovery of new molecules with anticancer properties. Compounds with cytolytic activity and the ability to induce immunogenic cell death can be administered as intratumoral injections with the aim to activate anti-tumor immune responses by causing the release of tumor antigens as well as damage-associated molecular patterns (DAMPs) from dying cancer cells. In the present study, we report the cytolytic and DAMP-releasing efects of a new natural product mimic termed MPM-1 that was inspired by the marine Eusynstyelamides. We found that MPM-1 rapidly killed cancer cells in vitro by inducing a necrosis-like death, which was accompanied by lysosomal swelling and perturbation of autophagy in HSC-3 (human oral squamous cell carcinoma) cells. MPM-1 also induced release of the DAMPs adenosine triphosphate (ATP) and high mobility group box 1 (HMGB1) from Ramos (B-cell lymphoma) and HSC-3 cells, as well as cell surface expression of calreticulin in HSC-3 cells. This indicates that MPM-1 has the ability to induce immunogenic cell death, further suggesting that it may have potential as a novel anticancer compound

Effects of functional ingredients on gut inflammation in Atlantic salmon (Salmo salar L)

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Different Dietary Ratios of Camelina Oil to Sandeel Oil Influence the Capacity to Synthesise and Deposit EPA and DHA in Zucker Fa/Fa Rats

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A Screening Study on Effects Varying Dietary Macronutrient Composition on Gut Functions in Lumpfish (Cyclopterus lumpus)

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