Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D.

Acne vulgaris is the most common skin disorder affecting millions of people worldwide and inflammation resulting from the immune response targeting Propionibacterium acnes has a significant role in its pathogenesis. In this study, we have demonstrated that P. acnes is a potent inducer of T helper 17 (Th17) and Th1, but not Th2 responses in human peripheral blood mononuclear cells (PBMCs). P. acnes stimulated expression of key Th17-related genes, including IL-17A, RORα, RORc, IL-17RA, and IL-17RC, and triggered IL-17 secretion from CD4(+), but not from CD8(+) T cells. Supernatants from P. acnes-stimulated PBMCs were sufficient to promote the differentiation of naive CD4(+)CD45RA T cells into Th17 cells. Furthermore, we found that the combination of IL-1β, IL-6, and transforming growth factor-β-neutralizing antibodies completely inhibited P. acnes-induced IL-17 production. Importantly, we showed that IL-17-expressing cells were present in skin biopsies from acne patients but not from normal donors. Finally, vitamin A (all-trans retinoic acid) and vitamin D (1,25-dihydroxyvitamin D3) inhibited P. acnes-induced Th17 differentiation. Together, our data demonstrate that IL-17 is induced by P. acnes and expressed in acne lesions and that both vitamin A and D could be effective tools to modulate Th17-mediated diseases such as acne

Graduation Rate Differences by Ethnicity/Race at Texas Community Colleges: A Statewide, Multiyear Examination

In this multiyear, statewide investigation, the degree to which differences were present in graduation rates between Black and White, Black and Hispanic, and Black and Asian students was addressed for the 2007-2008 academic year to the 2015-2016 academic years. Inferential statistical procedures revealed the presence of statistically significant differences for all 9 academic years. In all 9 years, Black students had statistically significantly lower graduation rates than White, Hispanic, and Asian students. Also identified were the Texas community colleges that had the highest and that had the lowest graduation rates of their Black students in the last two academic years. Implications of these findings and recommendations for future research were discussed

The Effects of Auditory Perception and Musical Preference on Anxiety in Naive Human Subjects

Background: The use of music as a method of relieving anxiety has been studied extensively by researchers from varying disciplines. The abundance of these reports focused on which genre of music best aided in the relief of stress. Little work has been performed in the area of auditory preference in an attempt to ascertain whether an individual’s preferred music type aids in their anxiety reduction at levels greater than music that they have little or no propensity for. Material/Methods: In the present report we seek to determine whether naive human subjects exposed to music of their preference show a decrease in anxiety, as measured by systolic and diastolic blood pressure values. We furthermore contrast these values to those obtained during non-preferred music listening. Results: We found statistically significant reduction of anxiety levels only when subjects were exposed to their preferred musical selections. Conclusions: Students participating in the study already had knowledge of what genre of music would best relax them. It is our belief, that within the general population, many people do not have this self understanding. We conclude that music therapy may provide a mechanism for this self-understanding and subsequently help alleviate anxiety and stress

Images and Spectral Performance of WFC3 Interference Filters

The Wide Field Camera 3 (WFC3) is a panchromatic imager that will be deployed in the Hubble Space Telescope (HST). The mission of the WFC3 is to enhance HST1s imaging capability in the ultraviolet, visible and near-infrared spectral regions. Together with a wavelength coverage spanning 2000A to 1.7 micron, the WFC3 high sensitivity, high spatial resolution, and large field-of-view provide the astronomer with an unprecedented set of tools for exploring all types of exciting astrophysical terrain and for addressing many key questions in astronomy today. The filter compliment, which includes broad, medium, and narrow band filters, naturally reflects the diversity of astronomical programs to be targeted with WFC3. The WFC3 holds 61 UVIS filters elements, 14 IR filters, and 3 dispersive elements. During ground testing, the majority of the UVIS filters were found to exhibit excellent performance consistent with or exceeding expectations; however, a subset of filters showed considerable ghost images; some with relative intensity as high as 10-15%. Replacement filters with band-defining coatings that substantially reduce these ghost images were designed and procured. A state-of-the-art characterization setup was developed to measured the intensity of ghost images, focal shift, wedge direction , transmitted uniformity and surface feature of filters that could effect uniform flat field images. We will report on this new filter characterization methods, as well as the spectral performance measurements of the in-band transmittance and blocking

3Rd-Order Nonlinearity Of 4-Dialkylamino-4\u27Nitro-Stilbene Wave-Guides At 1319 Nm

The intensity dependent optical properties of 4-dialkylamino-4\u27nitro-stilbene polymer channel waveguides were measured at 1319 nm with a pulse modulated Mach-Zehnder interferometer to be n2 = 0.8 x 10(-13) cm2/W and beta2 \u3c 0.08 cm/GW. This material is promising for all-optical switching at 1319 nm because it satisfies both the W and T figures of merit

Childhood obesity and adult cardiovascular disease risk factors: a systematic review with meta-analysis

Background Overweight and obesity is a major public health concern that includes associations with the development of cardiovascular disease (CVD) risk factors during childhood and adolescence as well as premature mortality in adults. Despite the high prevalence of childhood and adolescent obesity as well as adult CVD, individual studies as well as previous systematic reviews examining the relationship between childhood obesity and adult CVD have yielded conflicting results. The purpose of this study was to use the aggregate data meta-analytic approach to address this gap. Methods Studies were included if they met the following criteria: (1) longitudinal and cohort studies (including case-cohort), (2) childhood exposure and adult outcomes collected on the same individual over time, (3) childhood obesity, as defined by the original study authors, (4) English-language articles, (5) studies published up to June, 2015, (6) one or more of the following CVD risk factors [systolic blood pressure (SBP), diastolic blood pressure (DBP), total cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), non-high-density lipoprotein cholesterol (non-HDL), and triglycerides (TG)], (7) outcome(s) not self-reported, and (8) exposure measurements (child’s adiposity) assessed by health professionals, trained investigators, or self-reported. Studies were retrieved by searching three electronic databases as well as citation tracking. Fisher’s r to z score was calculated for each study for each outcome. Pooled effect sizes were calculated using random-effects models while risk of bias was assessed using the STROBE instrument. In order to try and identify sources of heterogeneity, random-effects meta-regression was also performed. Results Of the 4840 citations reviewed, a total of 23 studies were included in the systematic review and 21 in the meta-analysis. The findings suggested that childhood obesity is significantly and positively associated with adult SBP (Zr = 0.11; 95% CI: 0.07, 0.14), DBP (Zr = 0.11; 95% CI: 0.07, 0.14), and TG (Zr =0.08; 95% CI: 0.03, 0.13), and significantly and inversely associated with adult HDL (Zr = −0.06; 95% CI: -0.10, −0.02). For those studies that adjusted for adult body mass index (BMI), associations were reversed, suggesting that adult BMI may be a potential mediator. Nine studies had more than 33% of items that placed them at an increased risk for bias. Conclusions The results of this study suggest that childhood obesity may be a risk factor for selected adult CVD risk factors. However, a need exists for additional, higher-quality studies that include, but are not limited to, both unadjusted and adjusted measures such as BMI before any definitive conclusions can be reached. Systematic review and meta-analysis PROSPERO 2015: CRD42015019763

A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200µm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics

Case Study Hydrologic Effects of Size and Location of Fields Converted from Drained Pine Forest to Agricultural Cropland

Abstract: Hydrological effects of land-use change are of great concern to ecohydrologists and watershed managers, especially in the Atlantic coastal plain of the southeastern United States. The concern is attributable to rapid population growth and the resulting pressure to develop forested lands. Many researchers have studied these effects in various scales, with varying results. An extended watershed-scale forest hydrologic model, calibrated with 1996–2000 data, was used to evaluate long-term hydrologic effects of conversion to agriculture (corn–wheat–soybean cropland) of a 29.5-km2 intensively managed pine-forested watershed in Washington County in eastern North Carolina. Fifty years of weather data (1951–2000) from a nearby weather station were used for simulating hydrology to evaluate effects on outflows, evapotranspiration, and water table depth compared with the baseline scenario. Other simulation scenarios were created for each of five different percentages (10, 25, 50, 75, and 100%) of land-use conversion occurring at upstream and downstream locations in the pine-forest watershed. Simulations revealed that increased mean annual outflow was significant (α 0.05) only for 100 % conversion from forest (261 mm) to agricultural crop (326 mm), primarily attributed to a reduction in evapotranspiration. Although high flow rates>5 mm day−1 increased from 2.3 to 2.6 % (downstream) and 2.6 to 4.2 % (upstream) for 25 to 50 % conversion, the frequency was higher for the upstream location than the downstream. These results were attributed to a substantial decrease in soil hydraulic conductivity of one of the dominant soils in the upstream location, which is expected after land-use conversion to agriculture. As a result, predicted subsurface drainage decreased, and surface runoff increased as soil hydraulic conductivity decreased for the soil upstream. These results indicate tha

Nuclear Cryogenic Propulsion Stage

The fundamental capability of Nuclear Thermal Propulsion (NTP) is game changing for space exploration. A first generation Nuclear Cryogenic Propulsion Stage (NCPS) based on NTP could provide high thrust at a specific impulse above 900 s, roughly double that of state of the art chemical engines. Characteristics of fission and NTP indicate that useful first generation systems will provide a foundation for future systems with extremely high performance. The role of the NCPS in the development of advanced nuclear propulsion systems could be analogous to the role of the DC-3 in the development of advanced aviation. Progress made under the NCPS project could help enable both advanced NTP and advanced NEP