Occurrence of Campylobacter in the genitals of teaser bulls maintained at an embryo transfer center

Em central de transferência de embriões, após os procedimentos de reconhecimento do cio em 37 vacas receptoras, através de quatro rufiões vasectomizados, observou-se que 83% delas apresentavam retorno ao cio e algum corrimento serofibrinoso. Nos exames bacteriológicos realizados nos lavados prepuciais dos rufiões foi isolado o Campylobacter fetus subsp. venerealis em todos, fato que, analisado associadamente com o retorno ao cio das vacas receptoras, é indicativo da ocorrência de campilobacteriose no plantel. Essa ocorrência demonstra a necessidade de medidas eficazes de planejamento de saúde animal, pela utilização de rufiões com desvio lateral do pênis. Uma vez impossibilitado o contato sexual, seria impedida a transmissão do agente durante o coito. Torna-se imperioso consignar que a prática da prevenção racional de enfermidades continua sendo o procedimento mais econômico para uma produtividade animal mais rentável

Occurrence of Campylobacter in the genitals of teaser bulls maintained at an embryo transfer center

Em central de transferência de embriões, após os procedimentos de reconhecimento do cio em 37 vacas receptoras, através de quatro rufiões vasectomizados, observou-se que 83% delas apresentavam retorno ao cio e algum corrimento serofibrinoso. Nos exames bacteriológicos realizados nos lavados prepuciais dos rufiões foi isolado o Campylobacter fetus subsp. venerealis em todos, fato que, analisado associadamente com o retorno ao cio das vacas receptoras, é indicativo da ocorrência de campilobacteriose no plantel. Essa ocorrência demonstra a necessidade de medidas eficazes de planejamento de saúde animal, pela utilização de rufiões com desvio lateral do pênis. Uma vez impossibilitado o contato sexual, seria impedida a transmissão do agente durante o coito. Torna-se imperioso consignar que a prática da prevenção racional de enfermidades continua sendo o procedimento mais econômico para uma produtividade animal mais rentável

Cisplatin-induced Cytogenetic Alterations In V79 Cells

Cisplatin is an antineoplastic agent used to treat solid malignancies, such as ovarian, testicular and bladder tumors. Studies have been shown that cisplatin induces genotoxic effects and chromosomal alterations that can result in genetic and chromosomal instability. In this work, we investigated the effects of cisplatin on the cytogenetic traits of cultured V79 cells based on the modal chromosome number, mitotic index, frequency of polyploidy and number of aneuploid metaphases. Cisplatin-treated V79 cells showed an altered chromosome number distribution, as well as an enhanced mitotic index, frequency of polyploidy and number of aneuploid metaphases. These alterations were probably related to the genetic instability produced by cisplatin. In addition, these cells showed characteristics associated with neoplastic development that corresponded to neoplastic processes related to the use of cisplatin in chemotherapy. © 2007 The Japan Mendel Society.722155160Allavena, P., Pirovano, P., Bonazzi, C., Colombo, N., Mantovani, A., D'Incalci, M., In vitro and in vivo effects of cisplatin on the generation of lymphokine-activated killer cells (1990) J. Natl. Cancer Inst, 82, pp. 139-142Barrett, J.C., Cell culture models of multistep carcinogenesis (1985) IARC Sci. Publ, 58, pp. 181-202Borenfreund, E., Babich, H., Martin-Alguacil, N., Effect of methylazoxymethanol acetate on bluegill sunfish cell cultures in vitro (1989) Ecotoxicol. Environ. Saf, 17, pp. 297-307Bradley, M.O., Bhuyan, B., Francis, M.C., Langenbach, R., Peterson, A., Huberman, E., Mutagenesis by chemical agents in V79 chinese hamster cells: A review and analysis of the literature. A report of the Gene-Tox Program (1981) Mutat. Res, 87, pp. 81-142Chambers, S.K., Chopyk, R.L., Chambers, J.T., Schwartz, P.E., Duffy, T.P., Development of leukemia after doxorubicin and cisplatin treatment for ovarian cancer (1989) Cancer, 64, pp. 2459-2461Chang, W., Little, J., Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation: Association with delayed reproductive death phenotype (1992) Mutat. Res, 270, pp. 191-199Deitch, A.D., Sawicki, S.G., Effects of cordycepin on microtubules of cultured mammalian cells (1979) Exp. Cell Res, 118, pp. 1-13Genari, S.C., Wada, M.L.F., Behavioural differences and cytogenetical analysis of a transformed cellular population derived from a Vero cell line (1995) Cytobios, 81, pp. 17-25Genari, S.C., Wada, M.L.F., Dolder, M.A.H., Wada, M.L.F., Scanning and transmission electron microscopy of transformed Vero cells, with altered in vitro growth characteristics (1996) J. Submicrosc. Cytol. Pathol, 28, pp. 565-572Genari, S.C., Wada, M.L.F., Dolder, M.A.H., Wada, M.L.F., Wada, M.L.F., Effects of nutritional stress conditions on the ploidy and growth of human amniotic cells (2000) In Vitro Cell. Develop. Biol, 36, pp. 147-150Genari, S.C., Wada, M.L.F., Dolder, M.A.H., Wada, M.L.F., Wada, M.L.F., The influence of nutritional stress on the differentiation of epithelial cells in vitro (2003) Braz. J. Morphol. Sci, 20, pp. 135-140Giaccone, G., Clinical perspectives on platinum resistance (2000) Drugs, 59, pp. 9-17Gilvarry, U., Farrel, D., Lynch, V., Moriarty, M., Dooley, M., Law, E., Clynes, M., Cytological differences between normal and malignant human cell populations in culture (1990) Cancer Res, 50, pp. 3390-3393González, V.M., Fuertes, M.A., Alonso, C., Pérez, J.M., Is cisplatin-induced cell death always produced by apoptosis? (2001) Mol. Pharmacol, 59, pp. 657-663Hadnagy, W., Seemayer, N.H., Cytotoxic and genotoxic effects of extract of particulate emission from a gasoline-powered engine (1988) Environ. Mol. Mutagen, 12, pp. 385-396Harper, K., Lorimore, S., Wright, E., Delayed appearance of radiation-induced mutations at the Hprt locus in murine hematopoietic cells (1997) Exp. Hematol, 25, pp. 263-269Holliday, R., Chromosome error propagation and cancer (1989) Trends Genet, 5, pp. 42-45Little, J., Induction of genetic instability by ionizing radiation (1999) C. R. Acad. Sci. III, 322, pp. 127-134Lothschutz, D., Jennewein, M., Pahl, S., Lausberg, H.F., Eichler, A., Mutschler, W., Hanselmann, R.G., Oberringer, M., Polyploidization and centrosome hyperamplification in inflammatory bronchi (2002) Inflamm. Res, 51, pp. 416-422Mead, G.M., Green, J.A., Macbeth, F.R., Williams, C.J., Whitehouse, J.M., Buchanan, R., Second malignancy after cisplatin, vinblastine, and bleomycin (PVB) chemotherapy: A case report (1983) Cancer Treat. Rep, 67, p. 410Ohshima, S., Induction of genetic instability and chromosomal instability by nickel sulfate in V79 Chinese hamster cells (2003) Mutagenesis, 18, pp. 133-137Reedjik, J., The mechanism of action of platinum anti-cancer drugs (1987) Pure Appl. Chem, 59, pp. 181-192Rosenberg, B., VanCamp, L., Trosko, J.E., Mansour, V.H., Platinum compounds: A new class of potent antitumor agents (1969) Nature, 222, pp. 385-386Srb, V., Prochazkova, J., Monitoring of effects of cis-diamminedichloroplatinum II. Part I. Cytogenetic analysis in vitro (1983) Neoplasma, 30, pp. 463-467Storchova, Z., Pellman, D., From polyploidy to aneuploidy, genome instability and cancer (2004) Nat. Rev. Mol. Cell. 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Synthesis and screening of libraries of synthetic tripodal receptor molecules with three different amino acid or peptide arms: identification of iron binders

A novel selectively deprotectable triazacyclophane scaffold was used for the design and split−mix synthesis of two libraries of solid-phase bound tripodal synthetic receptors possessing three different amino acid or peptidic arms. In the synthesis of the first library, the two outer arms consisted of amino acid Ala, Arg, Asp, Gln, Gly, Lys, Phe, Ser, Tyr, or Val and the middle arm consisted of amino acid Asn, Glu, His, Leu, or Pro. The second library contained amino acid and/or (di)peptide arms. The arms were different in all library members. The first outer arm consisted of amino acid(s) Ala, Arg, Gln, Phe, or Ser, the second outer arm consisted of amino acid(s) Asp, Gly, Lys, Tyr, or Val, and the middle arm consisted of amino acid(s) Asn, Glu, His, Leu, or Pro, leading to a 27 000 member library of synthetic tripodal receptor molecules. In on-bead screening experiments, a remarkable selectivity of some library members for Fe3+ was observed and decoding of their structures by Edman degradation revealed consensus sequences with structural resemblance to non-heme iron proteins

Production of galacto-oligosaccharides by the β-galactosidase from kluyveromyces lactis : comparative analysis of permeabilized cells versus soluble enzyme

8 páginas, 5 figuras, 3 tablas -- PAGS nros. 10477-10484The transgalactosylation activity of Kluyveromyces lactis cells was studied in detail. Cells were permeabilized with ethanol and further lyophilized to facilitate the transit of substrates and products. The resulting biocatalyst was assayed for the synthesis of galacto-oligosaccharides (GOS) and compared with two soluble β-galactosidases from K. lactis (Lactozym 3000 L HP G and Maxilact LGX 5000). Using 400 g/L lactose, the maximum GOS yield, measured by HPAEC-PAD analysis, was 177 g/L (44% w/w of total carbohydrates). The major products synthesized were the disaccharides 6-galactobiose [Gal-β(1→6)-Gal] and allolactose [Gal-β(1→6)-Glc], as well as the trisaccharide 6-galactosyl-lactose [Gal-β(1→6)-Gal-β(1→4)-Glc], which was characterized by MS and 2D NMR. Structural characterization of another synthesized disaccharide, Gal-β(1→3)-Glc, was carried out. GOS yield obtained with soluble β-galactosidases was slightly lower (160 g/L for Lactozym 3000 L HP G and 154 g/L for Maxilact LGX 5000); however, the typical profile with a maximum GOS concentration followed by partial hydrolysis of the newly formed oligosaccharides was not observed with the soluble enzymes. Results were correlated with the higher stability of β-galactosidase when permeabilized whole cells were usedProjects BIO2007-67708-C04-01, BIO2007-67708-C04-03, BIO2010-20508-C04-01, and BIO2010-20508-C04-04 from the Spanish Ministry of Science and Innovation supported this research. B.R.-C. and M.A.d.A. were supported by fellowships from the Spanish Ministries of Science and Innovation (FPI program) and Education and Culture (FPU program), respectivelyPeer reviewe