TEM study of homoepitaxial diamond layers scheduled for high power devices: FIB method of sample preparation

Homoepitaxial diamond structure observation by transmission electron microscopy (TEM) is still a very hard job due to the difficulty in preparing electron transparent samples for the further observation. The present contribution details the experimental operations with their respective conditions step by step. Finally high resolution TEM (HREM) observations of a CVD grown epilayer on a unnintentionally doped HPHT (001) oriented substrate are present to show the high quality of the sample preparation method.4 page

Review and classification of vision-based localisation techniques in unknown environments

International audienceThis study presents a review of the state-of-the-art and a novel classification of current vision-based localisation techniques in unknown environments. Indeed, because of progresses made in computer vision, it is now possible to consider vision-based systems as promising navigation means that can complement traditional navigation sensors like global navigation satellite systems (GNSSs) and inertial navigation systems. This study aims to review techniques employing a camera as a localisation sensor, provide a classification of techniques and introduce schemes that exploit the use of video information within a multi-sensor system. In fact, a general model is needed to better compare existing techniques in order to decide which approach is appropriate and which are the innovation axes. In addition, existing classifications only consider techniques based on vision as a standalone tool and do not consider video as a sensor among others. The focus is addressed to scenarios where no a priori knowledge of the environment is provided. In fact, these scenarios are the most challenging since the system has to cope with objects as they appear in the scene without any prior information about their expected position

Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells

Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.Fil: Wu, Ling. University of California at Los Angeles; Estados UnidosFil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados UnidosFil: Kyupelyan, Levon. University of California at Los Angeles; Estados UnidosFil: Latour, Brooke. University of California at Los Angeles; Estados UnidosFil: Gonzalez, Stephanie. University of California at Los Angeles; Estados UnidosFil: Shah, Saumya. University of California at Los Angeles; Estados UnidosFil: Galic, Zoran. University of California at Los Angeles; Estados UnidosFil: Ge, Sundi. University of California at Los Angeles; Estados UnidosFil: Zhu, Yuhua. University of California at Los Angeles; Estados UnidosFil: Petrigliano, Frank A.. University of California at Los Angeles; Estados UnidosFil: Nsair, Ali. University of California at Los Angeles; Estados UnidosFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Xinmin. University of California at Los Angeles; Estados UnidosFil: Lyons, Karen M.. University of California at Los Angeles; Estados UnidosFil: Crooks, Gay M.. University of California at Los Angeles; Estados UnidosFil: McAllister, David R.. University of California at Los Angeles; Estados UnidosFil: Van Handel, Ben. Novogenix Laboratories; Estados UnidosFil: Adams, John S.. University of California at Los Angeles; Estados UnidosFil: Evseenko, Denis. University of California at Los Angeles; Estados Unido

The impact of targeting TRAF2 and NCK-interacting protein kinase (TNIK) on anti-tumor effect in small cell lung cancer

View full abstracthttps://openworks.mdanderson.org/leading-edge/1056/thumbnail.jp

Anisotropic coarse-grained statistical potentials improve the ability to identify native-like protein structures

We present a new method to extract distance and orientation dependent potentials between amino acid side chains using a database of protein structures and the standard Boltzmann device. The importance of orientation dependent interactions is first established by computing orientational order parameters for proteins with alpha-helical and beta-sheet architecture. Extraction of the anisotropic interactions requires defining local reference frames for each amino acid that uniquely determine the coordinates of the neighboring residues. Using the local reference frames and histograms of the radial and angular correlation functions for a standard set of non-homologue protein structures, we construct the anisotropic pair potentials. The performance of the orientation dependent potentials was studied using a large database of decoy proteins. The results demonstrate that the new distance and orientation dependent residue-residue potentials present a significantly improved ability to recognize native folds from a set of native and decoy protein structures.Comment: Submitted to "The Journal of Chemical Physics

Developing a flexible automated continuous downstream processing system for research to clinical supply

Continuous manufacturing has gained a lot of attention over the last 10-15 years for numerous reasons such as the potential for higher efficiencies, reduced cost of goods, and improved product quality. However, the adoption of these technologies has been slow due to concerns over operating these processes in a GMP manufacturing environment. Some of these concerns relate to the operation of multiple continuous unit operations in an integrated process sequence. This presentation will highlight these concerns and show how these issues were addressed by developing an overarching automated and modular platform which can be easily reconfigured for processing most products. The developed automation platform is the result of a project funded by Innovate UK that brings together a number of biopharmaceutical companies including Allergan, AstraZeneca, Fujifilm Diosynth Biotechnologies and GSK to identify and address these issues. One objective of the project is to develop a flexible automated biologics downstream process consisting of multiple unit operations that can be rapidly reconfigured for manufacturing different products. To that end the process has been design with modularity in mind with each module having common inputs and outputs. The automation software has also been developed in a way that most typical downstream processes can be implemented in the system with little to no software updates. The ability to rapidly reconfigure the process has been demonstrated by using the system to produce three products with different process sequences. Another issue that inhibits the adoption of continuous technologies is the concern over simultaneously operating multiple unit operations. This presentation will detail how the automation software was developed to control both the key unit operations such as chromatography and filtration steps but also intermediate operations such as feed conditioning and viral inactivation steps. The automated system reduces the complexity of downstream processes, which can have in excess of eleven unit operations, to a single user-friendly interface. Implementing this control platform enables a single operator to control the entire process. This presentation will also detail how the automation strategy has been developed to enable a single operator to deal with start-up/shutdown, perturbations in the process and mid-process equipment turnover. It will highlight the challenges that have been faced when developing this system and how these have been overcome. The aim of this project was to improve efficiency by reducing processing time when compared to the current batch process and this was demonstrated by testing the system with three different products (a MAb and a MAb fusion protein). Furthermore, this presentation with show data from the production of three products that demonstrates comparability between the continuous process and the original batch processes. It will then detail how this was used to demonstrate the production of a large-scale clinical batch run

Basin tectonic history and paleo-physiography of the pelagian platform, northern Tunisia, using vitrinite reflectance data

Constraining the thermal, burial and uplift/exhumation history of sedimentary basins is crucial in the understanding of upper crustal strain evolution and also has implications for understanding the nature and timing of hydrocarbon maturation and migration. In this study, we use Vitrinite Reflectance (VR) data to elucidate the paleo‐physiography and thermal history of an inverted basin in the foreland of the Atlasic orogeny in Northern Tunisia. In doing so, it is the primary aim of this study to demonstrate how VR techniques may be applied to unravel basin subsidence/uplift history of structural domains and provide valuable insights into the kinematic evolution of sedimentary basins. VR measurements of both the onshore Pelagian Platform and the Tunisian Furrow in Northern Tunisia are used to impose constraints on the deformation history of a long‐lived structural feature in the studied region, namely the Zaghouan Fault. Previous work has shown that this fault was active as an extensional structure in Lower Jurassic to Aptian times, before subsequently being inverted during the Late Cretaceous Eocene Atlas I tectonic event and Upper Miocene Atlas II tectonic event. Quantifying and constraining this latter inversion stage, and shedding light on the roles of structural inheritance and the basin thermal history, are secondary aims of this study. The results of this study show that the Atlas II WNW‐ESE compressive event deformed both the Pelagian Platform and the Tunisian Furrow during Tortonian‐Messinian times. Maximum burial depth for the Pelagian Platform was reached during the Middle to Upper Miocene, i.e. prior to the Atlas II folding event. VR measurements indicate that the Cretaceous to Ypresian section of the Pelagian Platform was buried to a maximum burial depth of ~3 km, using a geothermal gradient of 30°C/km. Cretaceous rock samples VR values show that the hanging wall of the Zaghouan Fault was buried to a maximum depth of <2 km. This suggests that a vertical km‐scale throw along the Zaghouan Fault pre‐dated the Atlas II shortening, and also proves that the fault controlled the subsidence of the Pelagian Platform during the Oligo‐Miocene. Mean exhumation rates of the Pelagian Platform throughout the Messinian to Quaternary were in the order of 0.3 mm/year. However, when the additional effect of Tortonian‐Messinian folding is accounted for, exhumation rates could have reached 0.6-0.7 mm/year

Validating DLL3-targeting CAR T in small cell lung cancer

View full abstracthttps://openworks.mdanderson.org/leading-edge/1022/thumbnail.jp

The walkthrough method : an approach to the study of apps

Software applications (apps) are now prevalent in the digital media environment. They are the site of significant sociocultural and economic transformations across many domains, from health and relationships to entertainment and everyday finance. As relatively closed technical systems, apps pose new methodological challenges for sociocultural digital media research. This paper describes a method, grounded in a combination of science and technology studies with cultural studies, through which researchers can perform a critical analysis of a given app. The method involves establishing an app’s environment of expected use by identifying and describing its vision, operating model, and modes of governance. It then deploys a walkthrough technique to systematically and forensically step through the various stages of app registration and entry, everyday use, and discontinuation of use. The walkthrough method establishes a foundational corpus of data upon which can be built a more detailed analysis of an app’s intended purpose, embedded cultural meanings, and implied ideal users and uses. The walkthrough also serves as a foundation for further user-centred research that can identify how users resist these arrangements and appropriate app technology for their own purposes

Ursinus College Alumni Journal, November 1961

The president\u27s wife writes • Mater Ursini • The Ursinus College European tour • Admiral Moreell looks at a philosopher • Zucker looks at Moreell • A woman\u27s approach to peace • Founders\u27 Day • 1962 Forum programs • Cutting campus • From Alaska to Greenland • Faith and freedom in Taiwan • Reginald Helfferich honored • Poet King • Henry P. Laughlin, \u2738 • Robert Pease, \u2733 • Ursinus student at White House conference • Harleston R. Wood • Football • Soccer • Hockey • Basketball • Wrestling • 53.5% contribute to Loyalty Fund in 1961 • Progress report 1962 campaign • 1961 campaign results • The Century Club • Loyalty Fund all-stars • Loyalty Fund kick-off dinner • Matching gifts • Results of the 1961 Loyalty Fund campaign • Contributors to the 1961 Loyalty Fund campaign • Class notes • Weddings • Births • Necrology • Sing along with us • New faculty membershttps://digitalcommons.ursinus.edu/alumnijournal/1072/thumbnail.jp