Understanding Late Pleistocene Landscapes of Central Italy: a Multidisciplinary Approach

It is now clear in archaeology that a full interpretation of sites cannot be realised if information about the environmental setting and constraints of the surrounding context are not available. The Latium coast (central Italy) has been extensively investigated since the 19th century and is one of the regions with the highest number of prehistoric sites in Italy. In spite of this, multidisciplinary environmental reconstructions of this region are still absent. This thesis deals with investigations of the travertine quarry Cava Muracci (Latium, central Italy), where excavations were carried out between 2012 and 2016. Seven caves were discovered, coprolites, a large faunal assemblage and a small lithic collection were found at one of them (i.e. Area 3) which was revealed to be a cave hyena den dating between 44–34 ka BP. A holistic palaeoecological study has been undertaken to increase our knowledge of the environment of the coastal Latium, the so-called Pontine Plain. Pollen analysis of cave hyena coprolites, an extremely undervalued resource, has been carried out for the first time in the region. This study has provided new insights into the vegetation and climate of the Pontine Plain, previously known only through distant pollen records. The faunal assemblage from the den has also been exhaustively studied. The environmental inferences have then been combined with the pollen data and geological information. The results returned a complex reconstruction of the local landscape, with at least three main habitats and a wide biodiversity. Finally, the role of the Pontine Plain as an ecological refugium has been examined. The results of this research suggest the region had milder environmental constraints, providing an optimal place to live to several faunal and vegetational taxa also during the harshest millennia of Late Pleistocene

Role of the irrigation charges to induce the adoption of water saving innovation in semi-arid regions

The paper investigates about the effectiveness of water charges in inducing farmers to adopt the technical innovation aimed at water saving. It is claimed that by increasing water charge, the signal of the scarcity of the water resource is directly and effectively conveyed to farmers, who are supposed to promptly react by adopting a water saving technology. The analysis is referred to two types of innovation: an agronomic innovation, consisting on a crop mulching practice, and a management innovation, based on a voluntarily water pricing scheme with tariffs differentiated according to a peak and off-peak season. A theoretical model based on farms’ profit maximization is proposed, to evaluate the trigger conditions for the innovation. The model is applied to a case study referred to a semi-arid region, located in the South of Italy, according to which there is no clear evidence that a generalized increase may induce farmers to adopt the innovatio

Irrigation pricing policy aimed at the enhancement of water saving innovation at farm level. A case study

In this study we analyze the price inducement effect of a generalized water tariff increase in the adoption of water saving innovation at farm level. We apply a linear programming decision making model to analyze the determinants affecting the adoption of two types of innovation: a process innovation, consisting on a crop mulching practice, and a management innovation, based on a voluntary water seasonal pricing scheme, differentiated according to a peak and off-peak periods. According to our results, the mulching practice is not affected by the tariffs that are still lower than the water marginal productivity, while they exert a negative effect when they become higher. On the contrary, the adoption of the voluntary water seasonal pricing scheme is affected by the tariffs increase only if the latter induce an excessive concentration of water demand during the peakDans cette étude nous présentons une recherche relative à la possibilité que l augmentation de tarif de l eau est capable d induire l agriculteur de adopter une innovation adressée à l épargne de l eau. Notre modèle de programmation linéaire nous a permis de analyser le procès de décision de l agriculteur et d entendre les causes déterminantes de l adoption de deux typologies d innovation: une innovation de procès, relative à une technique de paillis, et une innovation de management, relative à l introduction d un plan volontaire, avec des tarifs différentes entre les periodes de intense et de normale utilisation de l eau. Nos résultats montre que l augmentation de tarif de l eau n est pas une mesure sufficiente pour stimuler les agriculteurs à adopter la technique de paillis, lorsque le tarif de l eau est inférieur à sa productivité marginale. Ou contraire, quand le tarif est plus grand que la productivité marginale de l eau, l augmentation de tarif freine l adoption de l innovation. L introduction des tarifs différentes entre les periodes de intense ed de normale utilisation de l eau est encouragée par l augmentation de tarif seulement quand la demande de l eau est plus concentrée dans la la periode de intense utilisation de l ea

Glyco-metabolic Effects of Ranolazine: A truly Multifaceted Drug?

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SerpinB3 and Yap Interplay Increases Myc Oncogenic Activity

SerpinB3 has been recently described as an early marker of liver carcinogenesis, but the potential mechanistic role of this serpin in tumor development is still poorly understood. Overexpression of Myc often correlates with more aggressive tumour forms, supporting its involvement in carcinogenesis. Yes-associated protein (Yap), the main effector of the Hippo pathway, is a central regulator of proliferation and it has been found up-regulated in hepatocellular carcinomas. The study has been designed to investigate and characterize the interplay and functional modulation of Myc by SerpinB3 in liver cancer. Results from this study indicate that Myc was up-regulated by SerpinB3 through calpain and Hippo-dependent molecular mechanisms in transgenic mice and hepatoma cells overexpressing human SerpinB3, and also in human hepatocellular carcinomas. Human recombinant SerpinB3 was capable to inhibit the activity of Calpain in vitro, likely reducing its ability to cleave Myc in its non oncogenic Myc-nick cytoplasmic form. SerpinB3 indirectly increased the transcription of Myc through the induction of Yap pathway. These findings provide for the first time evidence that SerpinB3 can improve the production of Myc through direct and indirect mechanisms that include the inhibition of generation of its cytoplasmic form and the activation of Yap pathway

Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream

The present study was designed to evaluate the effects of dietary levels of bioactive peptides (BPs) derived from salmon processing by-products on the presence and distribution of peptic cells (oxyntopeptic cells, OPs) and enteric endocrine cells (EECs) that contain GHR, NPY and SOM in the gastric mucosa of European seabass and gilthead seabream. In this study, 27 seabass and 27 seabreams were divided into three experimental groups: a control group (CTR) fed a control diet and two groups fed different levels of BP to replace fishmeal: 5% BP (BP5%) and 10% BP (BP10%). The stomach of each fish was sampled and processed for immunohistochemistry. Some SOM, NPY and GHR-IR cells exhibited alternating “open type” and “closed type” EECs morphologies. The BP10% group (16.8 ± 7.5) showed an increase in the number of NPY-IR cells compared to CTR (CTR 8.5 ± 4.8) and BP5% (BP10% vs. CTR p ≤ 0.01; BP10% vs. BP5% p ≤ 0.05) in the seabream gastric mucosa. In addition, in seabream gastric tissue, SOM-IR cells in the BP 10% diet (16.8 ± 3.5) were different from those in CTR (12.5 ± 5) (CTR vs. BP 10% p ≤ 0.05) and BP 5% (12.9 ± 2.5) (BP 5% vs. BP 10% p ≤ 0.01). EEC SOM-IR cells increased at 10% BP (5.3 ± 0.7) compared to 5% BP (4.4 ± 0.8) (5% BP vs. 10% BP p ≤ 0.05) in seabass. The results obtained may provide a good basis for a better understanding of the potential of salmon BPs as feed ingredients for seabass and seabream.publishedVersio

In vitro study of uptake and synthesis of creatine and its precursors by cerebellar granule cells and astrocytes suggests some hypotheses on the physiopathology of the inherited disorders of creatine metabolism

<p>Abstract</p> <p>Background</p> <p>The discovery of the inherited disorders of creatine (Cr) synthesis and transport in the last few years disclosed the importance of blood Cr supply for the normal functioning of the brain. These putatively rare diseases share a common pathogenetic mechanism (the depletion of brain Cr) and similar phenotypes characterized by mental retardation, language disturbances, seizures and movement disorders. In the effort to improve our knowledge on the mechanisms regulating Cr pool inside the nervous tissue, Cr transport and synthesis and related gene transcripts were explored in primary cultures of rat cerebellar granule cells and astrocytes.</p> <p>Methods</p> <p>Cr uptake and synthesis were explored in vitro by incubating monotypic primary cultures of rat type I astrocytes and cerebellar granule cells with: a) D₃-Creatine (D₃Cr) and D3Cr plus β-guanidinopropionate (GPA, an inhibitor of Cr transporter), and b) labelled precursors of Guanidinoacetate (GAA) and Cr (Arginine, Arg; Glycine, Gly). Intracellular D3Cr and labelled GAA and Cr were assessed by ESI-MS/MS. Creatine transporter (<it>CT1</it>), L-arginine:glycine amidinotransferase (<it>AGAT</it>), and S-adenosylmethionine:guanidinoacetate N-methyltransferase (<it>GAMT</it>) gene expression was assessed in the same cells by real time PCR.</p> <p>Results</p> <p>D3Cr signal was extremely high in cells incubated with this isotope (labelled/unlabelled Cr ratio reached about 10 and 122, respectively in cerebellar granule cells and astrocytes) and was reduced by GPA. Labelled Arg and Gly were taken up by the cells and incorporated in GAA, whose concentration paralleled that of these precursors both in the extracellular medium and inside the cells (astrocytes). In contrast, the increase of labelled Cr was relatively much more limited since labelled Cr after precursors' supplementation did not exceed 2,7% (cerebellar granule cells) and 21% (astrocytes) of unlabelled Cr. Finally, <it>AGAT, GAMT </it>and <it>SLC6A8 </it>were expressed in both kind of cells.</p> <p>Conclusions</p> <p>Our results confirm that both neurons and astrocytes have the capability to synthesize and uptake Cr, and suggest that at least in vitro intracellular Cr can increase to a much greater extent through uptake than through <it>de novo </it>synthesis. Our results are compatible with the clinical observations that when the Cr transporter is defective, intracellular Cr is absent despite the brain should be able to synthesize it. Further research is needed to fully understand to what extent our results reflect the in vivo situation.</p

Living to fight another day : The ecological and evolutionary significance of Neanderthal healthcare

Evidence of care for the ill and injured amongst Neanderthals, inferred through skeletal evidence for survival from severe illness and injury, is widely accepted. However, healthcare practices have been viewed primarily as an example of complex cultural behaviour, often discussed alongside symbolism or mortuary practices. Here we argue that care for the ill and injured is likely to have a long evolutionary history and to have been highly effective in improving health and reducing mortality risks. Healthcare provisioning can thus be understood alongside other collaborative ‘risk pooling’ strategies such as collaborative hunting, food sharing and collaborative parenting. For Neanderthals in particular the selective advantages of healthcare provisioning would have been elevated by a variety of ecological conditions which increased the risk of injury as well their particular behavioural adaptations which affected the benefits of promoting survival from injury and illness. We argue that healthcare provisioning was not only a more significant evolutionary adaptation than has previously been acknowledged, but moreover may also have been essential to Neanderthal occupation at the limits of the North Temperate Zone

pMineR: An Innovative R Library for Performing Process Mining in Medicine

Process Mining is an emerging discipline investigating tasks related with the automated identification of process models, given realworld data (Process Discovery). The analysis of such models can provide useful insights to domain experts. In addition, models of processes can be used to test if a given process complies (Conformance Checking) with specifications. For these capabilities, Process Mining is gaining importance and attention in healthcare. In this paper we introduce pMineR, an R library specifically designed for performing Process Mining in the medical domain, and supporting human experts by presenting processes in a human-readable way

Risk of Covid-19 severe outcomes and mortality in migrants and ethnic minorities compared to the general population in the european WHO region. A systematic review

The Covid-19 pandemic has had a major impact on migrants and ethnic minorities (MEMs). Socio-economic factors and legal, administrative and language barriers are among the reasons for this increased susceptibility. The aim of the study is to investigate the impact of Covid-19 on MEMs compared to the general population in terms of serious outcomes. We conducted a systematic review collecting studies on the impact of Covid-19 on MEMs compared to the general population in the WHO European Region regarding hospitalisation, intensive care unit (ICU) admission and mortality, published between 01/01/2020 and 19/03/2021. Nine researchers were involved in selection, study quality assessment and data extraction. Of the 82 studies included, 15 of the 16 regarding hospitalisation for Covid-19 reported an increased risk for MEMs compared to the white and/or native population and 22 out of the 28 studies focusing on the ICU admission rates found an increased risk for MEMs. Among the 65 studies on mortality, 43 report a higher risk for MEMs. An increased risk of adverse outcomes was reported for MEMs. Social determinants of health are among the main factors involved in the genesis of health inequalities: a disadvantaged socio-economic status, a framework of structural racism and asymmetric access to healthcare are linked to increased susceptibility to the consequences of Covid-19. These findings underline the need for policymakers to consider the socio-economic barriers when designing prevention plans