ORAL MANIFESTATIONS IN PEDIATRIC PATIENTS RECEIVING CHEMOTHERAPY FOR LEUKEMIA

Cross-sectional study of 36 children with acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML). Intraoral examination and oral hygiene assessment were performed in all patients. Information was also obtained from interviews of patients’ mothers and a review of medical records. Results: The sample was largely composed of boys (58.3%), ages 5–9 years. The most commonly used chemotherapy regimen was the BFM-95 protocol. Most children (83.3%) had a record of some oral manifestation during treatment. On intraoral examination, 17 children (50%) were found to have at least one oral manifestation. The most frequent manifestation was also mucositis (26.5%), followed by gingival bleeding (23.4%). Lower maternal educational level was associated with increased frequency of mucositis, as were induction chemotherapy and maintenance chemotherapy after relapse. Conclusions: Oral abnormalities were common in children receiving chemotherapy and mucositis was the most prevalent manifestation. Oral lesions were associated with the induction phase of chemotherapy. Mucositis was not associated with oral health status

Evaluation of effect of a vitamin-based barrier cream on the clinical severity of actinic cheilitis : a preliminary study

Actinic Cheilitis (AC) is a pathological condition of the labial mucosa considered potentially malignant. The aim of this study was to investigate the effect of treatment of AC with daily use of a vitamin-based barrier cream. For this clinical study, 36 participants with lower-lip AC were recruited from three oral medicine services. At baseline, participants were evaluated by clinical examination and clinical severity of AC was classified as grade I to IV. All participants were dispensed a tube of a barrier cream containing vitamins A, D, E and ZnO to apply once nightly for 90 consecutive days. Monthly follow-up was performed to reclassify AC clinial severity. The primary outcome of interest was clinical remission of AC at 90-day follow-up compared to baseline. Progressive remission of AC lesions was observed as early as the first month and throughout the assessment period (p = 0.000). The 3-month period was insufficient for remission of lesions, especially among male participants (p = 0.002) and with a longer sun exposure in years (p = 0.007). Daily use of the vitamin-based barrier cream had a promissing positive impact on the severity of actinic cheilitis. However, a 90-day course of treatment was not sufficient to achieve lesions remission. The findings of this study suggest a promising new avenue for the treatment of lower-lip AC

Padronização do isolamento das células-tronco de polpa dentária para aplicação em engenharia de tecido ósseo

O tecido pulpar dentário tem sido descrito como fonte de células multipotentes, apresentam capacidade de autorrenovação e diferenciação em diferentes tipos celulares. Na terapia de tecidos essas células são promissoras. O objetivo do estudo foi isolar células-tronco mesenquimais de polpa dentária (CTMPD). O estudo foi composto por doadores entre 12 e 26 anos. As células foram isoladas a partir da polpa dentária obtida dos terceiros molares inclusos superiores. No isolamento, expansão e caracterização das CTMPDs o tecido foi acondicionado em placas de cultura, com meio específico completo e suplementado e mantidas em temperatura adequada até aderirem e atingirem a confluência de 80 %. Após, as células foram mantidas nas condições do início do isolamento e apresentaram 2 % da capacidade de formação de colônias. É possível concluir que as células-tronco da polpa dentária adulta são capazes de formar colônias e proliferar in vitro

Relationship between anemia and oral cancer : a case-control study

The aim of this study was to investigate the occurrence, type and severity of anemia at the time of diagnosis of oral cancer, and its potential association with the degree of tumor cell differentiation. This case-control study used 366 medical records of patients treated at two referral centers for oral cancer diagnosis, specifically: cases (n=70) with a histopathological diagnosis of oral squamous cell carcinoma (OSCC) of the oral cavity, and controls (n=296) with benign oral lesions. Sociodemographic, behavioral, and clinical variables of both groups, as well as complete blood count values, were analyzed by descriptive statistics and crude/adjusted logistic regression. Anemia was detected in 15.7% of the cases and 11.8% of the controls. The presence of anemia had an OR=1.64 (odds ratio) (95%CI 0.54–5.00) for OSCC, with no significantly statistical association. Normocytic anemia was the most prevalent form of anemia when oral cancer was diagnosed (91.4% of the controls and 72.7% of the cases), and moderate to severely low hemoglobin levels were associated with OSCC diagnosis (OR 6.49; 95%CI 1.18–35.24), albeit data on hematological examinations were missing

Evaluation of Aquaporins 1 and 5 Expression in Rat Parotid Glands After Volumetric Modulated Arc Radiotherapy and Use of Low-Level Laser Therapy at Different Times

Introduction: This experimental study investigated the mRNA expression of aquaporins (AQPs) 1 and 5 in the parotid glands of rats irradiated with volumetric modulated arc therapy (VMAT) and subjected to low-level laser therapy (LLLT) at different time points.Methods: The sample consisted of 30 Wistar rats (Rattus norvegicus) divided into the following groups: control, LLLT alone (LG), radiotherapy alone (RG), and experimental groups that received LLLT at 24 hours (early experimental group [EEG], n=12) and 120 hours (late experimental group [LEG], n=12) after radiotherapy. VMAT was delivered at a single dose (12 Gy) and LLLT was performed with an aluminium-gallium-arsenide diode laser (660 nm, 100 mW), spot area of 0.0028 cm2, energy of 2 J/cm2 applied to 3 spots in the region corresponding to the right parotid gland, for 10 consecutive days. The right parotid gland was resected and prepared for RNA extraction. The gene expression of AQPs was evaluated by quantitative polymerase chain reaction (qPCR) using specific TaqMan probes, with the HPRT gene as an internal control.Results: The lowest AQP1 gene expression was 0.83 (0.27) with the use of LLLT 24 hours after radiotherapy (EEG), and the highest was 1.56 (0.80) with the use of LLLT alone (LG). Likewise, the lowest AQP5 gene expression was found in the EEG (mean = 0.88; SD = 0.49) and the highest in the LG (mean = 1.29; SD = 0.33).Conclusion: The use of LLLT after radiotherapy may contribute to the maintenance and an increase of these proteins, even when used at a later time point after radiotherapy

Lymphangioma of the oral cavity: Case report

The lymphangiomas are hamarthomatous lesions of the limphatic vases. There are benign proliferations that envolve the lymphatic system has a predilection for the head, neck and oral cavity. They represent benign proliferations that involve the lymphatic system, has a predilection for the head, neck and oral cavity. The prognostic for the linfangiomas located in the tongue is good for the most of the patients, although the voluminous lesions can cause obstruction of the aerial ways and consequently the death. The purpose of this study is to report two clinical cases of the lymphangioma located on the tongue, as well as to revise the pertinent literature focusing the clinical aspects and therapeutic possibilities

Displasia cleidocraniana: aspectos clínicos e radiográficos e relato de um caso clínico

Cleidocranial dysplasia is characterized by multiple abnormalities, mainly those related to craniofacial and corporal skeleton, besides oral and dental anomaly. The aim of this paper is to report a case of a female patient, 25 years old, who has characteristical signs of this pathology. General aspects of the disease, as well clinical and radiographic findings are also discussed and empathized, due to its rare occurrence.A Displasia Cleidocraniana (DCC) é caracterizada por múltiplas anormalidades, principalmente aquelas pertencentes ao esqueleto craniofacial e corporal, e também anormalidades buco-dentais. O objetivo deste artigo é apresentar o caso de uma paciente, gênero feminino, 25 anos, portadora de sinais característicos dessa entidade patológica. Os aspectos gerais da doença, assim como os sinais clínicos e radiográficos da síndrome são também discutidos e enfatizados, dada a sua rara ocorrência