Characterization and Evaluation of the Probiotic Properties of the Sporeforming Bacteria, \u3ci\u3eBacillus coagulans\u3c/i\u3e Unique IS-2

Probiotics are live organisms, that when consumed in adequate amounts, confer a health benefit to the host. To achieve probiotic status, each potential strain’s functional properties and their safety to the consumer must be comprehensively evaluated. Probiotic effects have been observed to be strain specific, therefore each new strain of interest must be characterized according to their phenotypic and genetic characteristics. There is a list of characteristics that potential probiotic strains should have to be considered as a probiotic. Potential probiotic strains should be evaluated for their acid and bile salt resistance, antimicrobial activity and adherence and colonization to intestinal cells in the lower gastrointestinal tract. Additionally, those that may be used as additives to food products should be evaluated for their survival during processing and storage of those products. The most common, and most researched, probiotic strains belong to the Lactobacillus and Bifidobacteriumgenera. However, there have been many reports of low viability of these strains in the delivery product by the end of the shelf life. This can cause problems as there is a desired level of probiotics that needs to be consumed to promote health benefits on the host. This problem has led the probiotic industry to research the probiotic properties of sporeforming organisms. Spores are dormant structures that are more resistant to heat, cold, acidity and desiccation. This inherent protection could help improve the survival of the organism in the product and through transit of the gastrointestinal tract. Bacillus coagulansis a Gram positive, lactic acid producing, facultative anaerobe and sporeforming bacteria strain that is drawing the attention of the probiotic industry. While research is still limited on this organism, there have been studies showing improvement of irritable bowel syndrome, Clostridium difficile induced colitis, rheumatoid arthritis and major depressive disorders associated with irritable bowel syndrome. Additionally, reports have shown the ability of Bacillusspores to reach and germinate in the gut, which is an important characteristic of potential probiotic strains. The purpose of this research is to evaluate the probiotic properties of the commercial Bacillus coagulans Unique IS-2, for its potential inclusion in food products. Advisor: Andreia Bianchin

Proceedings of the USDA-ARS workshop "Real world" infiltration

Compiled and edited by L.R. Ahuja and Amy Garrison.Includes bibliographical references.Proceedings of the 1996 workshop held on July 22-25, 1996 in Pingree Park, Colorado

Activist Agriculture: Farm protest in Iowa, 1929-1969

Throughout the twentieth century, farmers in Iowa and the Midwest struggled to make a living off their land and hard work. Post-war price busts and the Great Depression only exacerbated the general erosion in farm parity as increasing costs of production were not matched by an increase in farm commodity prices. In response, farmers organized in an effort to move from being victims of the economic and social situation to self-determined shapers of action. In this exhibit, we take a closer look at mobilization of farmers to confront and obstruct tuberculosis testing of cattle during the Iowa Cow Wars of the early 1930s and the commodity holding actions of the National Farmers Organization (NFO) in the 1960s. The exhibit will cover the actions and methods used by the farmers as well as how they leveraged the media to affect change. Finally, we look at the parallel struggles of migrant farm workers to improve wages and working conditions, examining the efforts both to pass legislation in Iowa in support of migrant farm workers and to support the Delano Grape Strike in California and the related international boycott.https://lib.dr.iastate.edu/speccoll_exhibits/1003/thumbnail.jp

Exploring Differences in Measurement and Reporting of Classroom Observation Inter-Rater Reliability

Although inter-rater reliability is an important aspect of using observational instruments, it has received little theoretical attention. In this article, we offer some guidance for practitioners and consumers of classroom observations so that they can make decisions about inter-rater reliability, both for study design and in the reporting of data and results. We reviewed articles in two major journals in the fields of reading and mathematics to understand how researchers have measured and reported inter-rater reliability in a recent decade. We found that researchers have tended to report measures of inter-rater agreement above the .80 threshold with little attention to the magnitude of score differences between raters. Then, we conducted simulations to understand both how different indices for classroom observation reliability are related to each other and the impact of reliability decisions on study results. Results from the simulation studies suggest that mean correlations with an outcome are slightly lower at lower levels of percentage of exact agreement but that the magnitude of score differences has a more dramatic effect on correlations. Therefore, adhering to strict thresholds for inter-rater agreement is less helpful than reporting exact point estimates and also examining measures of rater consistency. Accessed 2,893 times on https://pareonline.net from April 05, 2018 to December 31, 2019. For downloads from January 1, 2020 forward, please click on the PlumX Metrics link to the right

Patient experience and challenges in group concept mapping for clinical research.

BACKGROUND AND OBJECTIVE: Group concept mapping (GCM) is a research method that engages stakeholders in generating, structuring and representing ideas around a specific topic or question. GCM has been used with patients to answer questions related to health and disease but little is known about the patient experience as a participant in the process. This paper explores the patient experience participating in GCM as assessed with direct observation and surveys of participants. METHODS: This is a secondary analysis performed within a larger study in which 3 GCM iterations were performed to engage patients in identifying patient-important outcomes for diabetes care. Researchers tracked the frequency and type of assistance required by each participant to complete the sorting and rating steps of GCM. In addition, a 17-question patient experience survey was administered over the telephone to the participants after they had completed the GCM process. Survey questions asked about the personal impact of participating in GCM and the ease of various steps of the GCM process. RESULTS: Researchers helped patients 92 times during the 3 GCM iterations, most commonly to address software and computer literacy issues, but also with the sorting phase itself. Of the 52 GCM participants, 40 completed the post-GCM survey. Respondents averaged 56 years of age, were 50% female and had an average hemoglobin A1c of 9.1%. Ninety-two percent (n = 37) of respondents felt that they had contributed something important to this research project and 90% (n = 36) agreed or strongly agreed that their efforts would help others with diabetes. Respondents reported that the brainstorming session was less difficult when compared with sorting and rating of statements. DISCUSSION: Our results suggest that patients find value in participating in GCM. Patients reported less comfort with the sorting step of GCM when compared with brainstorming, an observation that correlates with our observations from the GCM sessions. Researchers should consider using paper sorting methods and objective measures of sorting quality when using GCM in patient-engaged research to improve the patient experience and concept map quality

A computerized adaptive testing system for speech discrimination measurement: The Speech Sound Pattern Discrimination Test

A computerized, adaptive test-delivery system for the measurement of speech discrimination, the Speech Sound Pattern Discrimination Test, is described and evaluated. Using a modified discrimination task, the testing system draws on a pool of 130 items spanning a broad range of difficulty to estimate an examinee\u27s location along an underlying continuum of speech processing ability, yet does not require the examinee to possess a high level of English language proficiency. The system is driven by a mathematical measurement model which selects only test items which are appropriate in difficulty level for a given examinee, thereby individualizing the testing experience. Test items were administered to a sample of young deaf adults, and the adaptive testing system evaluated in terms of respondents\u27 sensory and perceptual capabilities, acoustic and phonetic dimensions of speech, and theories of speech perception. Data obtained in this study support the validity, reliability, and efficiency of this test as a measure of speech processing ability

Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression

Little cigars (LCs) are regulated differently than cigarettes, allowing them to be potentially targeted at youth/young adults. We exposed human bronchial epithelial cultures (HBECs) to air or whole tobacco smoke from cigarettes vs. LCs. Chronic smoke exposure increased the number of dead cells, lactate dehydrogenase release, and interleukin-8 (IL-8) secretion and decreased apical cilia, cystic fibrosis transmembrane conductance regulator (CFTR) protein levels, and transepithelial resistance. These adverse effects were significantly greater in LC-exposed HBECs than cigarette exposed cultures. LC-exposure also elicited unique gene expression changes and altered the proteomic profiles of airway apical secretions compared to cigarette-exposed HBECs. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that LCs produced more chemicals than cigarettes, suggesting that the increased chemical load of LCs may be the cause of the greater toxicity. This is the first study of the biological effects of LCs on pulmonary epithelia and our observations strongly suggest that LCs pose a more severe danger to human health than cigarettes

Towards Accurate Differential Diagnosis with Large Language Models

An accurate differential diagnosis (DDx) is a cornerstone of medical care, often reached through an iterative process of interpretation that combines clinical history, physical examination, investigations and procedures. Interactive interfaces powered by Large Language Models (LLMs) present new opportunities to both assist and automate aspects of this process. In this study, we introduce an LLM optimized for diagnostic reasoning, and evaluate its ability to generate a DDx alone or as an aid to clinicians. 20 clinicians evaluated 302 challenging, real-world medical cases sourced from the New England Journal of Medicine (NEJM) case reports. Each case report was read by two clinicians, who were randomized to one of two assistive conditions: either assistance from search engines and standard medical resources, or LLM assistance in addition to these tools. All clinicians provided a baseline, unassisted DDx prior to using the respective assistive tools. Our LLM for DDx exhibited standalone performance that exceeded that of unassisted clinicians (top-10 accuracy 59.1% vs 33.6%, [p = 0.04]). Comparing the two assisted study arms, the DDx quality score was higher for clinicians assisted by our LLM (top-10 accuracy 51.7%) compared to clinicians without its assistance (36.1%) (McNemar's Test: 45.7, p < 0.01) and clinicians with search (44.4%) (4.75, p = 0.03). Further, clinicians assisted by our LLM arrived at more comprehensive differential lists than those without its assistance. Our study suggests that our LLM for DDx has potential to improve clinicians' diagnostic reasoning and accuracy in challenging cases, meriting further real-world evaluation for its ability to empower physicians and widen patients' access to specialist-level expertise

Taxonomy of the order Bunyavirales : second update 2018

In October 2018, the order Bunyavirales was amended by inclusion of the family Arenaviridae, abolishment of three families, creation of three new families, 19 new genera, and 14 new species, and renaming of three genera and 22 species. This article presents the updated taxonomy of the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).Non peer reviewe

2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology (2021) 166:3567–3579. https://doi.org/10.1007/s00705-021-05266-wIn March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.This work was supported in part through Laulima Government Solutions, LLC prime contract with the US National Institute of Allergy and Infectious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC under Contract No. HHSN272201800013C. This work was also supported in part with federal funds from the National Cancer Institute (NCI), National Institutes of Health (NIH), under Contract No. 75N91019D00024, Task Order No. 75N91019F00130 to I.C., who was supported by the Clinical Monitoring Research Program Directorate, Frederick National Lab for Cancer Research. This work was also funded in part by Contract No. HSHQDC-15-C-00064 awarded by DHS S&T for the management and operation of The National Biodefense Analysis and Countermeasures Center, a federally funded research and development center operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowledges partial support from the Special Research Initiative of Mississippi Agricultural and Forestry Experiment Station (MAFES), Mississippi State University, and the National Institute of Food and Agriculture, US Department of Agriculture, Hatch Project 1021494. Part of this work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001030), the UK Medical Research Council (FC001030), and the Wellcome Trust (FC001030).S