7,191 research outputs found

    Assessing the Influence of Living and Working Conditions on Alcohol Consumption in Migrant Farmworkers in Mexico

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    Background and Purpose: Inadequate working and living conditions are associated with alcohol consumption in farmworkers in the U.S. However, the influence of these factors on alcohol consumption patterns in migrant farmworkers in Mexico remains unclear. The purpose of this analysis was to assess the influence of housing and working conditions on alcohol use in migrant farmworkers in Mexico. Methods: We used logistic and ordinal logistic regression to examine the association of living and working conditions on alcohol consumption and frequency in 3,132 farmworkers in Mexico with data from a Mexican national farmworker’s survey. Results: Living in inadequately built homes (OR=0.84; 95% CI=0.72, 0.98; p<0.05) and limited access to luxury items (OR=0.69; 95% CI=0.52, 0.94; p<0.01), were associated with a decreased likelihood to consume alcohol compared to living in better conditions. In contrast, living in employer provided housing (OR=1.79; 95% CI=1.40, 2.31; p<0.0001) and experiencing hazards related to safety (OR= 1.69; 95% CI= 1.35, 2.12; p<0.0001), work organization (OR= 1.29; CI= 1.03, 1.63; p<0.05), and ergonomics (OR= 2.04; CI= 1.18, 3.52; p<0.05) increased the likelihood of consuming alcohol. Conclusion: Living and working conditions of farmworkers may affect alcohol consumption. However, these findings require replication and specific mechanisms, which may influence these results warrants investigation

    Storage Temperature Affects Müller Glia Susceptibility to Hypoxia

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    Background/Objective: We conducted this project to differentiate cell susceptibility to hypoxia (low O2 level) between immortalized Müller glia (MIO-M1) stored in -80 versus liquid nitrogen. Method: MIO-M1 cells were cultured and seeded in 12 well plates at 40K/well and in 6 well plates at 75K/well until confluence. Cell culture conditions: centrifugation time and speed, neutralization, and storage temperature were varied. Cells were subjected to chemically induced hypoxia by treatment with 300 and 400uM CoCl2 for 24 and 48hours. Oxygen level was measured in cells, cells were imaged and counted. Results: Centrifugation and neutralization conditions did not affect cell survival. We found that storing MIO-M1 cell in -80 makes these cells more susceptible to death by hypoxia. First, we found that %live MIO-M1 cells stored in liquid nitrogen is near 100% compared to ~60% live cells when stored in -80. Second, cells stored in -80 showed significant susceptibility to chemically induced hypoxia compared to cells stored in liquid nitrogen that survived treated for 24 and 48 hours. Conclusion: We concluded that storage of these cells in liquid nitrogen maintain survival during hypoxic events. Cells’ susceptibility to hypoxia indicates that storage in -80 affect glycolysis efficiency in these cells to maintain survival. During hypoxia, mitochondrial is dysfunctional as they depend on O2 for ATP production during oxidative phosphorylation. Cells stored in liquid nitrogen survived hypoxic conditions, which may indicate that MIO-M1 depends on glycolysis for ATP production. Further investigation will proceed to determine Müller glia metabolism preferenc

    Termoquímica em quadrinhos : uma ferramenta didática para o ensino de conceitos químicos abordados no nível médio

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    Os estudantes, além de aprenderem os conteúdos no contexto escolar, também podem aprender no contexto de seu cotidiano. Alguns termos, como por exemplo, calor, têm significados distintos quando comparados os conceitos científicos com os cotidianos. A partir dessa observação, Vygotsky (2008) diz que o ensino desses conceitos deve mostrar aos alunos as diferenças de significados, mas trabalhando-se com ambos simultaneamente. Nesse sentido de construção do conhecimento cientifico apresentado por Vygotsky, o objetivo deste trabalho é elaborar uma história em quadrinhos (HQ) para ensinar os conceitos introdutórios da termoquímica, aplicar essa HQ em sala aula e analisar como a HQ pode contribuir para a compreensão desses conceitos por um grupo de alunos do ensino médio. A introdução dos conceitos básicos da termoquímica utilizando os quadrinhos, ou seja, a união de imagens e textos, deve facilitar a aprendizagem dos conteúdos. Não foi encontrado, nas bases de dados pesquisadas, nenhum trabalho com a utilização de HQ no ensino de termoquímica no nível médio. Num primeiro momento foi elaborada a HQ, seguindo-se sua aplicação em sala de aula. Procedeu-se da seguinte forma: primeiro aplicou-se um questionário para saber qual o entendimento prévio dos alunos quanto aos conceitos de termoquímica; depois realizou-se a leitura dos quadrinhos e os experimentos propostos; na terceira parte os alunos avaliaram a HQ através de um questionário; e, por fim, responderam a um questionário sobre os conceitos fundamentais da termoquímica trabalhados em aula. Esse estudo pode ser classificado como uma pesquisa de caráter qualitativo, particularmente um estudo de caso, realizado em uma escola pública de Canoas/RS, totalizando a participação de 49 alunos do segundo ano do ensino médio. Observou-se que menos da metade dos alunos gostam de ler HQs, que não as tinham usado em outras aulas e que a maioria achou que a linguagem dos quadrinhos facilitou no entendimento do conteúdo. Pode-se dizer que a utilização de HQs nas aulas de Química contribuiu positivamente para a compreensão dos conceitos termoquímicos.Students, in addition to learning contents in the school context, can also learn in the context of their daily lives. Some words, as heat for example, have different meanings when compared scientific to everyday concepts. Based on this observation, Vygotsky (2008) says that the teaching of these concepts must show students their differences in meanings, but working with both simultaneously. In this sense of building the scientific knowledge presented by Vygotsky, the aim of this work is to develop a comic book (HQ) to teach the introductory concepts of thermochemistry, apply this HQ in the classroom and analyze how HQ can contribute to the understanding of these concepts by a group of high school students. The introduction of the basic concepts of thermochemistry using comics, that is, the union of images and texts should facilitate the learning of the contents. No work was found in the researched databases using comic books in the teaching of thermochemistry at the secondary level. This study can be classified as a qualitative research, particularly a case study, conducted in a public school in Canoas/RS, with the participation of 49 students in the second year of high school. At first, the author elaborated a comic book, then it was applied in the classroom. We proceeded as follows: a questionnaire was applied to find out what were the previous students’ understanding about thermochemistry concepts; then the comic book was read and the proposed experiments were carried out; in the third part the students evaluated the comics through a questionnaire; and finally answered a questionnaire on the fundamental concepts of thermochemistry. It was observed that less than half of the students like to read comics, that they had not used them in other classes, and most felt that the language of the comics facilitated the understanding of the content. It can be said that the use of comic books in chemistry classes contributed positively to the understanding of thermochemical concepts

    Control de los Costos Ocultos para la Eficiencia del Desempeño Organizacional de una Empresa de Mantenimiento

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    La presente Tesis titulada “Control de los costos ocultos para la eficiencia del desempeño organizacional de una empresa de mantenimiento” tuvo como finalidad el de aumentar la eficiencia de la empresa Epiroc Perú S.A. a razón de ello se generó la siguiente pregunta ¿Cuál es la influencia del control de los costos ocultos en la eficiencia del desempeño organizacional en una empresa de mantenimiento?. Esta investigación tuvo como objetivo principal determinar que el control de los costos ocultos si influye en el desempeño organizacional de una empresa de mantenimiento. El método de investigación es inductivo – deductivo, el tipo de investigación es aplicada, el nivel de investigación es descriptivo – explicativo, el diseño es cuasi experimental, para ello se contó con una muestra de tipo no probabilístico, siendo la empresa Epiroc Perú S.A. La técnica para la recolección de datos en esta tesis fue la observación directa y los instrumentos utilizados fueron los análisis de datos del reporte diario y encuestas. Se concluye que efectivamente existe una influencia de los costos ocultos sobre el desempeño organizacional, llegándose a demostrar estadísticamente que los resultados obtenidos son favorables por el incremento de la disponibilidad de los equipos en promedio del 44% con respecto a la primera toma de datos

    Effect of Chitosan Edible Coating on the Biochemical and Physical Characteristics of Carp Fillet (Cyprinus carpio) Stored at −18ºC.

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    The effect of an edible coating (EC) with 1.5% chitosan as an additive, on common carp (Cyprinus carpio) fillet, was determined evaluating the biochemical, physicochemical, textural, microbiological, and nutritional characteristics periodically during its hydroperoxides content (HPC) (0.8324 nM hydroperoxides/mg of protein versus 0.5540 nM/mg with regard to the EC sample), as well as protein carbonyl content (PCC) (0.5860 nM versus 0.4743 nM of reactive carbonyl groups/mg of protein of noncoated material), keeping properties for a longer period of time, and a lower protein solubility (7.8 mg of supernatant protein/mg of total microbialgrowth,thecountwas2.2×10 CFU/gofsampleinmesophilesversus4.7×10 intheECsample.Theresultsindicatethat the use of EC added with chitosan maintains the quality of the product regarding lipid and protein oxidation until fourth month of storage, maintaining moisture content without variation for at least 3 months, and inhibits microbial growth up to 2 logarithmic units, during five months of frozen storage

    Insect chitosan as a natural antimicrobial against vegetative cells of Bacillus cereus in a cooked rice matrix

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    [EN] This study investigates the antimicrobial activity of insect chitosan against vegetative cells of Bacillus cereus in a rice matrix. Sample culture solutions were prepared with different concentrations of insect chitosan (150, 180, 220 and 250 ¿g/mL) and tested at three temperatures (30 ¿C, 20 ¿C and 10 ¿C), which simulate different storage temperature scenarios of precooked rice. The results indicate that insect chitosan has antimicrobial activity that depends on temperature and chitosan concentration. For the assays with chitosan at 10 ¿C, all concentrations were bactericidal during the study time, reaching a maximum inactivation of 6 log cycles for 250 ¿g/mL. At 20 ¿C and at 30 ¿C a bacteriostatic activity was observed for concentrations of 150 ¿g/mL and 180 ¿g/mL. Results also showed that concentrations of 220 ¿g/mL and 250 ¿g/mL were bactericidal for all the temperatures tested during the storage time. When rice is cooked and not stored at an appropriate temperature, below 10 ¿C, the consumer¿s health is at risk. In these cases, insect chitosan could be a good additional control measure to control B. cereus growth and toxin formation in cooked rice.We want to thank TRACE-RICE project, Reference Number AMD-1934-1 and grant PID 2020-116318RB-C31 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe", the support for this Research.Valdez, MI.; Garcia, J.; Ubeda-Manzanaro, M.; Martínez López, A.; Rodrigo, D. (2022). Insect chitosan as a natural antimicrobial against vegetative cells of Bacillus cereus in a cooked rice matrix. Food Microbiology. 107:1-5. https://doi.org/10.1016/j.fm.2022.1040771510

    Use of Pleurotus pulmonarius to change the nutritional quality of wheat straw. I. effect on chemical composition

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    The effect of Pleurotus pulmonarius on the chemical composition of wheat straw was evaluated. Wheat straw, treated and untreated with P. pulmonarius, was obtained from a commercial facility. Ten samples plastic bags of wheat straw used previously as substrate to culture edible fungus were collected at random. The negative control group consisted of the pasteurized wheat straw untreated with P. pulmonarius. All samples were analyzed to determine dry matter, organic matter, crude protein, neutral detergent fiber, acid detergent fiber, cellulose and hemicellulose of each wheat straw. Data were analyzed by mean comparison using a t-Student test. No differences (P>0.05) between treatments were found for dry matter, crude protein and hemicellulose; however, straw treated with P. pulmonarius showed higher percentages (P<0.05) of organic matter, neutral and acid detergent fiber. It is concluded that growing P. pulmonarius in wheat straw improves the chemical composition of the straw by increasing its organic matter content and modifies the fiber structure, which increases the soluble carbohydrates content
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