Geographical distribution of Austrolebias monstrosus (Huber 1995), A. elongatus (Steindachner 1881) and A. vandenbergi(Huber 1995) (Teleostei: Cyprinodontiformes), with comments on the biogeography and ecology of Rivulidae in Pampasic and Chaco floodplains

After several collecting trips in Argentina and a revision of material deposited in national ichthyological collections we present a new record for Austrolebias elongatus from Gualeguaychú, in Entre Ríos Province, Argentina. Also, we present evidence on the erroneous record of Austrolebias monstrosus, and A. vandenbergi from Ituzaingó, Corrientes Province. Additional records from Salta Province are presented for those species. Material determined as A. elongatus from Santiago del Estero is also attributed to A. monstrosus. Therefore we restrict the distribution of these two species to Semi-arid Chaco Region in Argentina, Paraguay and Bolivia.Fil: Alonso, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Calviño, Pablo Andrés. Grupo de Estudio del Killi Club Argentino; ArgentinaFil: Teran, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Unidad Ejecutora Lillo. Fundación Miguel Lillo. Unidad Ejecutora Lillo; ArgentinaFil: Garcia, Ignacio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina. Grupo de Estudio del Killi Club Argentino; Argentin

Pleistocene-Holocene P. nigra traces on tufa archives in the Northern Meseta of the Iberian Peninsula

Molds preserved on travertines constitute exceptional species-informative material to reconstruct past vegetation. These are common on the High Ebro region. Here, the fluvial system erosion and karstification of the Mesozoic limestone since the Late Tertiary, origins extensive travertine complexes like the one studied in Tubilla del Agua (Burgos). In this site, three different travertine complexes are observed. The older one is represented by two disconnected edifices incised by the Valoria river. The age of this structure is established between > 300,000 and 240,000 yr BP, as from the U/Th dating on its base and top. The subsequent incision lasted until at least 90,000 yr BP, when another edifice erected upstream on the older one. The second travertine group comprises three platforms aged 60,000 yr BP the lower one, and 18,000 yr BP the middle one. The third travertine group is a lake-barrier complex 12,000 yr BP old, on which Tubilla del Agua was built, and a stratified travertine body that covers the valley bottom 10,000 to 2700 cal yr BP old. Some of the ages of the oldest travertines, and the most modern ones, coincide with the principal travertine formation cycles described by Durán (1996) for Spain. The matrix of precipitated calcium carbonate of all these complexes are rich in plant remain molds (mosses, stems, leaves, cones) of the surrounding flora. Pinus nigra Arnold cone molds have been localized and identified by comparative morphology studies. These remains are of high valuable information about the Pleistocene and Holocene presence of this taxon, in a site where no natural presence can be found today. This work was funded by project CGL2008-06005

Degradação de materiais oriundos dos implantes ou componentes e sua influência nos tecidos peri-implantares

Introdução: O recurso a implantes dentários assumiu um papel preponderante na reabilitação de pacientes edêntulos. O seu desempenho vai depender do tipo de materiais, tecidos circundantes, desenho do implante e as condições do ambiente oral. O titânio comercialmente puro é um dos materiais mais utilizado para tratamentos com implantes devido às suas propriedades, ainda assim, também pode causar problemas. Para procurar minorar algumas das limitações, outros materiais têm despertado a atenção, tal como a zircônia. O objetivo desta revisão sistemática foi avaliar a resistência à degradação e corrosão, resposta biológica de proliferação e adesão nos tecidos peri implantares nos materiais de titânio e zircônia. Materiais e métodos: A pesquisa da literatura foi realizada através da bases de dados MEDLINE (via PubMed) comparando os materias de zircônia e titânio, seguindo a estratégia de pesquisa PICO (Participantes/População, Intervenções, Comparação/Conteúdo, Outcome/resultados), para definir os critérios de inclusão e critérios de exclusão. Resultados: Em 4 dos 7 estudos incluidos confirma-se que os resultados obtidos são mais favoráveis para o grupo de zircônia no que diz respeito ao desgaste do material e corrosão que, por seu turno, também apresenta resposta biológicas á degradação comparável ao material de titânio. Conclusão: Com base nos artigos revistos pode ser constatado que os pilares de zircônia demonstraram melhores propriedades fisico-químicas de resistência ao desgaste e corrosão (tribocorrosão) além de melhor resposta biológica em comparação com os pilares de titânio.Introduction: The use of dental implants has assumed a major role in the rehabilitation of edentulous patients. Its performance will depend on the type of materials, surrounding tissues, design of the implant and the conditions of the oral environment. Commercially pure titanium is one of the most used materials for implant treatments due to its properties, yet it can also cause problems. In order to alleviate some of the limitations, other materials have attracted attention, such as zirconia. The objective of this systematic review was to evaluate the degradation and corrosion resistance and biological response of proliferation and adhesion in the peri-implant tissues of titanium and zirconia materials. Methods: The literature search was performed through the MEDLINE databases (via PubMed) comparing the materials of zirconia and titanium, following the search strategy PICO (Participants / Population, Interventions, Comparison / Content, Outcome ), for define inclusion criteria and exclusion criteria. Results: In 4 of the 7 studies included, it is confirmed that the results obtained are more favorable for the zirconia group with regard to material wear and corrosion which, in turn, also has a biological response to degradation comparable to titanium material. Conclusion: Based on the reviewed articles It can be seen that the zirconium abutments demonstrated better physico-chemical properties of resistance to wear and corrosion (tribocorrosion) in addition to a better biological response compared to titanium abutments

Occurrence of Astyanax dissensus Lucena & Thofehrn, 2013 (Teleostei: Characidae) in Argentina

The occurrence of Astyanax dissensus in the Uruguay River, at Yapeyú, Corrientes, Argentina is reported for the first time in the country.Fil: Teran, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Unidad Ejecutora Lillo. Fundación Miguel Lillo. Unidad Ejecutora Lillo; ArgentinaFil: Alonso, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Garcia, Ignacio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Calviño, Pablo. Grupo de Estudios del Killi Club Argentino; ArgentinaFil: Mirande, Juan Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Unidad Ejecutora Lillo. Fundación Miguel Lillo. Unidad Ejecutora Lillo; Argentin

The Prevention of Ischemia-Reperfusion Injury in Elderly Rats after Lower Limb Tourniquet Use

Background: Lower limb ischemia-reperfusion injury (IRI-LL) is a common major complication of orthopedic surgery, especially in elderly patients. It has previously been demonstrated that folinic acid (FA) reduced IRI-LL damage in 3–4-month-old rats. This current work analyses the effect of FA in the prevention of IRI-LL in elderly animals. Methods: Forty-two 18-month-old male WAG/RijHsd rats were subjected to 3 h of ischemia. Eighteen animals received FA (2.5 mg/kg, ip) 20 min before the end of the ischemia period, while the other half received the same volume of saline solution. The animals were sacrificed after 3 h, 24 h, and 14 days of reperfusion for biochemical (tissue damage markers and electrolytes), histopathological studies of the gastrocnemius muscle and the daily assessment of the limb function by the Rota Rod test, respectively. Results: The administration of FA prior to the end of the ischemia period reduced the increase in LDH and CK observed in non-treated animals by 30–40% (p < 0.0001). When the histological sections were analyzed, FA was found to have reduced the number of damaged muscle fibers per field by 20% (60 ± 17.1 vs. 80.7 ± 16.4, p < 0.0001). The functional test revealed that FA also led to an improvement in the muscle function, assessed by the length of time that the animals kept running on the rod, compared to untreated animals. Conclusions: The administration of FA, prior to the end of the ischemic period, decreases the damage induced by IRI-LL, also achieving a faster recovery of mobility.This research was funded by the University of the Basque Country UPV/EHU (grant reference GIU19/088)

Tailoring block copolymer nanoporous thin films with acetic acid as a small guest molecule

Block copolymers offer the fabrication of mesoporous thin films with distinct nanoscale structural features. In this contribution, we present the use of acetic acid (CH3COOH) as a low‐molecular‐weight guest molecule to tune the supramolecular assembly of poly[styrene‐block‐(4‐vinylpyridine)] (PS‐b‐P4VP), offering a versatile and straightforward method to obtain tailored nanostructured films with controlled topography and pore size. Spin‐coating toluene solutions of PS‐b‐P4VP, with a variable amount of CH3COOH, leads to micellar thin films, where the micelles contain the carboxylic acid as a guest molecule. The size can be conveniently modified in these films (from 48 to 75 nm) by varying the amount of organic acid in the starting solutions. Subsequent surface reconstruction of micellar films using ethanol leads to ring‐shaped copolymer nanoporous films with modulated diameter. Controlling the micelle reconstruction process, cylindrical porous films are also obtained. Interestingly, changing the type of aliphatic carboxylic acid leads to a modification of the observed film morphology from micelles to out‐of‐plane P4VP cylinders (or lamellae) in a PS matrix

Validation of a computer-adaptive test to evaluate generic health-related quality of life

<p>Abstract</p> <p>Background</p> <p>Health Related Quality of Life (HRQoL) is a relevant variable in the evaluation of health outcomes. Questionnaires based on Classical Test Theory typically require a large number of items to evaluate HRQoL. Computer Adaptive Testing (CAT) can be used to reduce tests length while maintaining and, in some cases, improving accuracy. This study aimed at validating a CAT based on Item Response Theory (IRT) for evaluation of generic HRQoL: the CAT-Health instrument.</p> <p>Methods</p> <p>Cross-sectional study of subjects aged over 18 attending Primary Care Centres for any reason. CAT-Health was administered along with the SF-12 Health Survey. Age, gender and a checklist of chronic conditions were also collected. CAT-Health was evaluated considering: 1) feasibility: completion time and test length; 2) content range coverage, Item Exposure Rate (IER) and test precision; and 3) construct validity: differences in the CAT-Health scores according to clinical variables and correlations between both questionnaires.</p> <p>Results</p> <p>396 subjects answered CAT-Health and SF-12, 67.2% females, mean age (SD) 48.6 (17.7) years. 36.9% did not report any chronic condition. Median completion time for CAT-Health was 81 seconds (IQ range = 59-118) and it increased with age (p < 0.001). The median number of items administered was 8 (IQ range = 6-10). Neither ceiling nor floor effects were found for the score. None of the items in the pool had an IER of 100% and it was over 5% for 27.1% of the items. Test Information Function (TIF) peaked between levels -1 and 0 of HRQoL. Statistically significant differences were observed in the CAT-Health scores according to the number and type of conditions.</p> <p>Conclusions</p> <p>Although domain-specific CATs exist for various areas of HRQoL, CAT-Health is one of the first IRT-based CATs designed to evaluate generic HRQoL and it has proven feasible, valid and efficient, when administered to a broad sample of individuals attending primary care settings.</p

The association between the tumor immune microenvironments and clinical outcome in low-grade, early-stage endometrial cancer patients

Endometrial tumors show substantial heterogeneity in their immune microenvironment. This heterogeneity could be used to improve the accuracy of current outcome prediction tools. We assessed the immune microenvironment of 235 patients diagnosed with low-grade, early-stage endometrial cancer. Multiplex quantitative immunofluorescence was carried out tomeasure CD8, CD68, FOXP3, PD-1,and PD-L1markers, aswell as cytokeratin (CK), on tissuemicroarrays. Clustering results revealed five robust immune response patterns, each associated with specific immune populations, cell phenotypes, and cell spatial clustering.Most samples (69%) belonged to theimmune-desert subtype, characterized by lowimmune cell densities. Tumor-infiltrating lymphocyte (TIL)-rich samples (4%) displayed high CD8+ T-cell infiltration, as well as a high percentage of CD8/PD-1+ cells. Immune-exclusion samples (19%) displayed the lowest CD8+ infiltration combined with high PD-L1 expression levels in CK+ tumor cells. In addition, they demonstrated high tumor cell spatial clustering as well as increased spatial proximity of CD8+/PD-1+ andCK/PD-L1+ cells.FOXP3andmacrophage-rich phenotypes (3%and 4% of total samples) displayed relatively high levels of FOXP3+ regulatory T-cells and CD68+ macrophages, respectively. These phenotypes correlated with clinical outcomes, with immune-exclusion tumors showing an association with tumor relapse. When compared with prediction models built using routine pathological variables, models optimized with immune variables showed increased outcome prediction capacity (AUC = 0.89 versus 0.78) and stratification potential. The improved prediction capacity was independent of mismatch repair protein status and adjuvant radiotherapy treatment. Further, immunofluorescence results could be partially recapitulated using single-marker immunohistochemistry (IHC) performed on whole tissue sections. TIL-rich tumors demonstrated increased CD8+ T-cells by IHC, while immune-exclusion tumors displayed a lack of CD8+ T-cells and frequent expression of PD-L1 in tumor cells. Our results demonstrate the capability of the immune microenvironment to improve standard prediction tools in low-grade, early-stage endometrial carcinomasCEA and IgM were funded by Fundación La Marató de TV3. This project was supported by grants from Partners of Choice Network from AstraZeneca and by the Instituto de Salud Carlos III (ISCIII) (PI17/01723 and PI21/00920), co-financed by the European Regional Development Fund ‘A way to achieve Europe’ (FEDER). We thank Marco Cassano (Lunaphore Technologies) for his help in writing the manuscrip