XUV digital in-line holography using high-order harmonics

A step towards a successful implementation of timeresolved digital in-line holography with extreme ultraviolet radiation is presented. Ultrashort XUV pulses are produced as high-order harmonics of a femtosecond laser and a Schwarzschild objective is used to focus harmonic radiation at 38 nm and to produce a strongly divergent reference beam for holographic recording. Experimental holograms of thin wires are recorded and the objects reconstructed. Descriptions of the simulation and reconstruction theory and algorithms are also given. Spatial resolution of few hundreds of nm is potentially achievable, and micrometer resolution range is demonstrated.Comment: 8 pages, 8 figure

A Quantitative 3D Motility Analysis of Trypanosoma brucei by Use of Digital In-line Holographic Microscopy

We present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form trypanosomes as well as mutant and wildtype cells under varying external conditions we were able to derive a general two-state-run-and-tumble-model for trypanosome motility. Differences in the motility of distinct strains indicate that adaption of the trypanosomes to their natural environments involves a change in their mode of swimming

Wavelength measurement with iterative self-imaging phenomenon

The wavelength of the electromagnetic radiation plays a key role on determining the spatial resolution an imaging system can achieve, the way how the wave interacts with matter and how it propagates. For this reason the measurement of the wavelength associated with electromagnetic radiation of any order is fundamental. For light, simple diffractioninterference experiments can lead to an appropriate measurement of this feature. For electrons, however, the wavelength value is calculated simply by the knowledge of the accelerating potential. In this work, we present an iterative method for measuring the wavelength of electromagnetic radiation of any order. By using the self-imaging effect that arises as an electromagnetic wave impinges on a periodic object, the wavelength of the wave can be determined. Experimental results of the application of the method to measure the wavelength of light are shown. The basis for the application of the method to determine the wavelength of electron waves are settled

Multi-wavelength digital in-line holographic microscopy

The use of multiple wavelength illumination in digital in-line holographic microscopy is presented. A biological sample is illuminated sequentially with three different optical sources and the separate holograms reconstructed with the appropriate wavelength. For controlling the scale of the reconstructed holograms, a reconstruction algorithm that allows for choosing the pixel size at the reconstruction plane independently of the wavelength and the reconstruction distance is used. The method is illustrated with experimental results. © OSA 2012

Chromatic aberration compensation in numerical reconstruction of digital holograms by Fresnel-Bluestein propagation

In this Letter, we present a method for chromatic compensation in numerical reconstruction of digitally recorded holograms based on Fresnel-Bluestein propagation. The proposed technique is applied to correct the chromatic aberration that arises in the reconstruction of RGB holograms of both millimeter-and micrometer-sized objects. The results show the feasibility of this strategy to remove the wavelength dependence of the size of the numerically propagated wavefields. (C) 2017 Optical Society of Americ