Debt management in Brazil : evaluation of the Real Plan and challenges ahead

Brazil's domestic debt has posed two challenges to policymakers: it has grown very fast and, despite progress, remains extremely short in maturity. The authors analyze Brazil's experience with domestic public debt management, searching for policy prescriptions for the next few years. After briefly reviewing the recent history of the country's domestic debt, they decompose the large rise in federal bonded debt in 1995-98, searching for its macroeconomic causes. The main explanations: extremely high interest payments (caused by Brazil's weak fiscal stance and quasi-fixed exchange rate regime) and the accumulation of assets (especially obligations of Brazil's states). Simulations of the net debt path for the near future underscore the importance of a tighter fiscal stance to prevent the debt-to-GDP ratio from growing further. The authors'main policy advice is to foster and rely more on inflation-linked bonds--the least harmful way to lengthen debt maturity.Economic Theory&Research,Banks&Banking Reform,Public Sector Economics&Finance,Payment Systems&Infrastructure,Strategic Debt Management,Economic Theory&Research,Banks&Banking Reform,Strategic Debt Management,Public Sector Economics&Finance,Municipal Financial Management

Chemically Distinct Nuclei and Outflowing Shocked Molecular Gas in Arp 220

We present the results of interferometric spectral line observations of Arp 220 at 3.5mm and 1.2mm from the Plateau de Bure Interferometer (PdBI), imaging the two nuclear disks in H$^{13}$CN$(1 - 0)$ and $(3 - 2)$, H$^{13}$CO$^+(1 - 0)$ and $(3 - 2)$, and HN$^{13}$C$(3 - 2)$ as well as SiO$(2 - 1)$ and $(6 - 5)$, HC$^{15}$N$(3 - 2)$, and SO$(6_6 - 5_5)$. The gas traced by SiO$(6 - 5)$ has a complex and extended kinematic signature including a prominent P Cygni profile, almost identical to previous observations of HCO$^+(3 - 2)$. Spatial offsets $0.1''$ north and south of the continuum centre in the emission and absorption of the SiO$(6 - 5)$ P Cygni profile in the western nucleus (WN) imply a bipolar outflow, delineating the northern and southern edges of its disk and suggesting a disk radius of $\sim40$ pc, consistent with that found by ALMA observations of Arp 220. We address the blending of SiO$(6 - 5)$ and H$^{13}$CO$^+(3 - 2)$ by considering two limiting cases with regards to the H$^{13}$CO$^+$ emission throughout our analysis. Large velocity gradient (LVG) modelling is used to constrain the physical conditions of the gas and to infer abundance ratios in the two nuclei. Our most conservative lower limit on the [H$^{13}$CN]/[H$^{13}$CO$^+$] abundance ratio is 11 in the WN, cf. 0.10 in the eastern nucleus (EN). Comparing these ratios to the literature we argue on chemical grounds for an energetically significant AGN in the WN driving either X-ray or shock chemistry, and a dominant starburst in the EN.Comment: 28 pages, 17 figures, accepted to Ap

Development and Evaluation of Fuzzy Logic Controllers for Improving Performance of Wind Turbines on Semi-Submersible Platforms under Different Wind Scenarios

Among renewable energy technologies, wind energy features one of the best possibilities for large-scale integration into power systems. However, there are specific restrictions regarding the installation areas for this technology, thus resulting in a growing, yet restricted, rate of penetration of the technology because of the limited viable sites onshore or in shallow waters. In this context, the use of offshore semi-submersible platforms appears as a promising option, which additionally enables the incorporation of other elements, such as wave energy converters or aquaculture. Nevertheless, this kind of offshore facility involves interactions between platform movements and the wind turbine, increasing the complexity of the system, causing traditional control techniques to not be able to fully cope with the dynamics of the system, and thus limiting the efficiency of energy extraction. On the contrary, the use of intelligent control techniques is an interesting option to take full account of the said interactions and to improve energy capture efficiency through the control of the pitch of the blades, especially under turbulent, above-rated wind profiles. This work presents an original fuzzy logic controller that has been validated by comparing it with previously validated controllers, following a developed methodology that allows comparison of controllers for wind turbines in semi-submersible platforms using performance indexes.This work was partially supported by the Ministry of Economy and Competitiveness (Government of Spain) and European Union (RTC-2016-5712-3); by the European Union, CDTI (Spain) and BEISS (UK) through the call H2020 ERA-NET DEMOWIND (WIP10+ project); by the Regional Government of Andalusia and European Union (UMA-CEIATECH-18); and finally, by partial funding for open access charge from the Universidad de Málaga. Partial funding for open access charge: Universidad de Málag

Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Comparisons of 2D and 3D cell culture models in literature have indicated differences in cellular morphology and metabolism, commonly attributed the better representation of in vivo conditions of the latter cell culture environment. Thus, interest in the use of 3D collagen gels for in vitro analysis has been growing. Although comparative studies to date have indicated an enhanced resistance of cells on collagen matrices against different toxicants, in the present study it is demonstrated that non-adapted protocols can lead to misinterpretation of results obtained from classical colorometric dye-based cytotoxic assays. Using the well established Alamar Blue assay, the study demonstrates how the transfer from 2D substrates to 3D collagen matrices can affect the uptake of the resazurin itself, affecting the outcome of the assay. Using flow cytometry, it is demonstrated that the cell viability is unaffected when cells are grown on collagen matrices, thus the difference seen in the fluorescence is a result of a dilution of the resazurin dye in the collagen matrix, and an increased uptake rate due to the larger cell surface exposed to the surrounding environment, facilitating more effective diffusion through the cellular membrane. The results are supported by a rate equation based simulation, verifying that differing uptake kinetics can result in apparently different cell viability. Finally, this work highlights the feasibility to apply classical dye-based assays on collagen based 3D cell culture models. However, the diffusion and bioavailbility of test substances in 3D matrices used in in vitro toxicological assays must be considered and adaption of the protocols is necessary for direct comparison with the traditional 2D models. Moreover, the observations made based on the resazurin dye can be applied to drugs or nanoparticles which freely diffuse through the collagen matrices, thus affecting the effective concentration exposed to the cells

The Dense Molecular Gas and Nuclear Activity in the ULIRG IRAS 13120-5453

We present new ALMA Band 7 ($\sim340$ GHz) observations of the dense gas tracers HCN, HCO$^+$, and CS in the local, single-nucleus, ultraluminous infrared galaxy IRAS 13120-5453. We find centrally enhanced HCN (4-3) emission, relative to HCO$^+$ (4-3), but do not find evidence for radiative pumping of HCN. Considering the size of the starburst (0.5 kpc) and the estimated supernovae rate of $\sim1.2$ yr$^{-1}$, the high HCN/HCO$^+$ ratio can be explained by an enhanced HCN abundance as a result of mechanical heating by the supernovae, though the active galactic nucleus and winds may also contribute additional mechanical heating. The starburst size implies a high $\Sigma_{IR}$ of $4.7\times10^{12}$ $L_{\odot}$ kpc$^{-2}$, slightly below predictions of radiation-pressure limited starbursts. The HCN line profile has low-level wings, which we tentatively interpret as evidence for outflowing dense molecular gas. However, the dense molecular outflow seen in the HCN line wings is unlikely to escape the galaxy and is destined to return to the nucleus and fuel future star formation. We also present modeling of Herschel observations of the H$_2$O lines and find a nuclear dust temperature of $\sim40$ K. IRAS 13120-5453 has a lower dust temperature and $\Sigma_{IR}$ than is inferred for the systems termed "compact obscured nuclei" (such as Arp 220 and Mrk 231). If IRAS 13120-5453 has undergone a compact obscured nucleus phase, we are likely witnessing it at a time when the feedback has already inflated the nuclear ISM and diluted star formation in the starburst/AGN core.Comment: accepted for publication in ApJ, 21 pages, 11 figure

A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum

We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, we have combined anterograde tracing using biotinylated dextran amine and retrograde neuroanatomical tracing with Fluoro-Gold. This dual tracing protocol was further implemented with the visualization of different subpopulations of striatal interneurons. The subsequent use of three different peroxidase substrates enabled us to unequivocally detect structures that were labeled within a three-color paradigm

Positive culture in allograft ACL-reconstruction: what to do?

The transmission of disease or infection from the donor to the recipient is always a risk with the use of allografts. We carried out a research study on the behavioural pattern of implanted allografts, which were initially stored in perfect conditions (all cultures being negative) but later presented positive cultures at the implantation stage. Because there is no information available on how to deal with this type of situation, our aim was to set guidelines on the course of action which would be required in such a case. We conducted a retrospective study of 181 patients who underwent an ACL reconstruction using BPTB allografts. All previous bone and blood cultures and tests for hepatitis B and C, syphilis and HIV were negative. An allograft sample was taken for culture in the operating theatre just before its implantation. The results of the cultures were obtained 3-5 days after the operation. We had 24 allografts with positive culture (13.25%) after the implantation with no clinical infection in any of these patients. Positive cultures could be caused by undetected contamination while harvesting, storing or during manipulation before implantation. The lack of clinical signs of infection during the follow-up of our patients may indicate that no specific treatment-other than an antibiotic protocol-would be required when facing a case of positive culture of a graft piece after its implantation

Observation of Multiplicity Dependent Prompt χc1 (3872) and ψ (2S) Production in pp Collisions

The production of χc1(3872) and ψ(2S) hadrons is studied as a function of charged particle multiplicity in pp collisions at a center-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 2 fb−1 . For both states, the fraction that is produced promptly at the collision vertex is found to decrease as charged particle multiplicity increases. The ratio of χc1(3872) to ψ(2S) cross-sections for promptly produced particles is also found to decrease with multiplicity, while no significant dependence on multiplicity is observed for the equivalent ratio of particles produced away from the collision vertex in b-hadron decays. This behavior is consistent with a calculation that models the χc1(3872) structure as a compact tetraquark. Implications for the binding energy of the χc1(3872) state are discussed