First bird footprints from the lower Miocene Lerín Formation, Ebro Basin, Spain

A new tracksite with bird footprints, found in the Bardenas Reales de Navarra Natural Park (Navarre, Spain), is presented in this study. The footprints are preserved in four sandstone blocks of the Lerín Formation from the northwest sector of the Ebro Basin. According to the magnetostratigraphic data, the age of these blocks is 20.4 Ma (Agenian, lower Miocene). The footprints are more than 100 mm in length, mesaxonic, and tridactyl, and have a prominent central pad impression with the digit impressions not jointed proximally. These features allow classifying them as Uvaichnites riojana. Some of the studied footprints are better preserved than the type series of Uvaichnites, which were found also in the northwest sector of the Ebro Basin. Therefore, the original diagnosis has been emended. Available chronostratigraphic data for these localities as well as for other footprints from China indicate a latest Oligocene-earliest Miocene age (from about 23 to 20 Ma) for Uvaichnites-like footprints. Sedimentological data also indicate similar continental environments, namely perilacustrine deltaic systems and distal alluvial systems. The information about early Miocene avian remains (bones, eggs and footprints) in the Iberian Peninsula is scarce. The skeletal and oological record of this age has been included within the families Phoenicopteridae, Phaisanidae and Cathartidae (or incertae sedis), while the ichnological record was related with trackmakers belonging to Charadriiformes, Ardeidae and Gruidae taxa. For this scenario, in which there are few avian remains, the ichnological diversity shown in this paper complements and improves the knowledge about the Iberian avian diversity in the early Miocene

Wireless sensor network-based system for measuring and monitoring road traffic

Nowadays, the measure and classification of vehicles in road traffic is accomplished by inductive loops placed under the pavement. These inductive loops allow monitoring vehicle passing by means of different configurations which provide us a number of data in order to control several parameters of the traffic (vehicle speed, traffic congestion and traffic accidents, between others). The major objective of this paper is to analysis an alternative to magnetic loops: the Wireless Sensor Networks. Firstly, a state of the art about road traffic control is described. Secondly, an alternative system based on Wireless Sensor Networks is analyzed. Network architecture for this WSN will be specified. It is not a trivial task because of the hard constraints of the small devices which compose the WSNs. In previous papers [1], we have proposed a methodology that facilitates the WSNs design for supporting real time applications such as traffic control applications. This design methodology has been used in order to obtain a WSN that reaches the real time requirements of a monitoring traffic application for intelligent roads. In the short term, the aim is to define a simulation model based on the designed WSN. To conclude, we have introduced a section about possible future directions in the smart roads field

TRHIOS: Trust and reputation in hierarchical and quality-oriented societies

In this paper we present TRHIOS: a Trust and Reputation system for HIerarchical and quality-Oriented Societies. We focus our work on hierarchical medical organizations. The model estimates the reputation of an individual, RTRHIOS, taking into account information from three trust dimensions: the hierarchy of the system; the source of information; and the quality of the results. Besides the concrete reputation value, it is important to know how reliable that value is; for each of the three dimensions we calculate the reliability of the assessed reputations; and aggregating them, the reliability of the reputation of an individual. The modular approach followed in the definition of the different types of reputations provides the system with a high flexibility that allows adapting the model to the peculiarities of each society

How Continuous Monitoring Changes the Interaction of Patients with a Mobile Telemedicine System

The use of continuous glucose monitor changes the way patients manage their diabetes, as observed in the increased number of daily insulin bolus, the increased number of daily BG measurements, and the differences in the distribution of BG measurements throughout the day. Continuous monitoring also increases the interaction of patients with the information system and modifies their patterns of use

Study of ostracod assemblage in recent sediments of ponds from Bardenas Reales de Navarra

En este trabajo se estudian dos sondeos realizados en dos balsas actuales (Cortinas y Piezarrey) de Bardenas Reales de Navarra, para determinar la evolución ambiental reciente de las dos balsas, que presentan una asociación de facies diferente. La balsa de las Cortinas muestra una mayor estabilidad en la lámina de agua hacia la actualidad, con el consecuente establecimiento de un medio cada vez más favorable para el desarrollo de ostrácodos. Actualmente, esta balsa está afectada por un agua cálida, mesohalina, ligeramente básica y con alto contenido en oxígeno disuelto, siendo abundante en la asociación viva Limnocythere inopinata. La balsa de Piezarrey se caracteriza por sufrir una colmatación en los últimos años, con la consiguiente disminución de la lámina de agua. Esto da origen a condiciones muy eutrofizadas que serían desfavorables para el mantenimiento de un ecosistema rico y diverso. La influencia en la actualidad de un agua cálida, oligohalina, básica y con alto contenido en oxígeno disuelto, favorece el predominio de Cypridopsis vidua en esta balsaIn this paper we study two cores recovered in two recent ponds (Cortinas and Piezarrey) from Bardenas Reales de Navarra to determine the recent environmental evolution of two ponds having a different facies association. The pond of Cortinas presents greatest stability in a water level through time, with the consequent establishment of the environment for the development of ostracods. Recently, this pond is affected by warm,mesohaline and slightly basic water with high dissolved oxygen content, being abundant Limnocythere inopinata in the living assemblage. The pond of Piezarrey is characterized by having a silting during the last years and the consequent decrease in the water level. This generates very eutrophicathes conditions that would be unfavorable to the maintenance of a rich and diverse ecosystem. The recent influence of warm, oligohaline and basic water with high dissolved oxygen content favors the dominance of Cypridopsis vidua in this pon

Up2B2: Playing English grammar games at the B2 level

The study presented herein is framed within the Mobile Assisted Language Learning (MALL) approach and presents the results from the use of an original mobile app, developed by the research team, on the part of 73 students. The app prototype consists of multiple choice questions and answers that assess varied aspects of the grammar, vocabulary and use of English at level B2 according to the Common European Framework of Reference for Languages (CEFRL). The application presents motivating gamification components that aim at enhancing students’ participation and regular use of the game. Some of these gamification features are response time, use of competition and training tests, and a score ranking based on nicknames. The study uses different indexes in order to describe the use of the platform and qualitative and quantitative indicators to reach positive conclusions related to students’ increased motivation and improvement in their grammar and vocabulary competence levels

Low Ciprofloxacin concentrations select Multidrug-Resistant Mutants overproducing efflux pumps in clinical isolates of Pseudomonas Aeruginosa

Low antibiotic concentrations present in natural environments are a severe and often neglected threat to public health. Even if they are present below their MICs, they may select for antibiotic-resistant pathogens. Notably, the minimal subinhibitory concentrations that select resistant bacteria, and define the respective sub-MIC selective windows, differ between antibiotics. The establishment of these selective concentrations is needed for risk-assessment studies regarding the presence of antibiotics in different habitats. Using short-term evolution experiments in a set of 12 Pseudomonas aeruginosa clinical isolates (including high-risk clones with ubiquitous distribution), we have determined that ciprofloxacin sub-MIC selective windows are strain specific and resistome dependent. Nonetheless, in all cases, clinically relevant multidrug-resistant (MDR) mutants emerged upon exposure to low ciprofloxacin concentrations, with these concentrations being below the levels reported in ciprofloxacin-polluted natural habitats where P. aeruginosa can be present. This feature expands the conditions and habitats where clinically relevant quinolone-resistant mutants can emerge. In addition, we established the lowest concentration threshold beyond which P. aeruginosa, regardless of the strain, becomes resistant to ciprofloxacin. Three days of exposure under this sub-MIC "risk concentration" led to the selection of MDR mutants that displayed resistance mechanisms usually ascribed to high selective pressures, i.e., the overproduction of the efflux pumps MexCD-OprJ and MexEF-OprN. From a One-Health viewpoint, these data stress the transcendent role of low drug concentrations, which can be encountered in natural ecosystems, in aggravating the antibiotic resistance problem, especially when it comes to pathogens of environmental origin. IMPORTANCE It has been established that antibiotic concentrations below MICs can select antibiotic-resistant pathogens, a feature of relevance for analyzing the role of nonclinical ecosystems in antibiotic resistance evolution. The range of concentrations where this selection occurs defines the sub-MIC selective window, whose width depends on the antibiotic. Herein, we have determined the ciprofloxacin sub-MIC selective windows of a set of Pseudomonas aeruginosa clinical isolates (including high-risk clones with worldwide distribution) and established the lowest concentration threshold, notably an amount reported to be present in natural ecosystems, beyond which this pathogen acquires resistance. Importantly, our results show that this ciprofloxacin sub-MIC selects for multidrug-resistant mutants overproducing clinically relevant efflux pumps. From a One-Health angle, this information supports that low antimicrobial concentrations, present in natural environments, may have a relevant role in worsening the antibiotic resistance crisis, particularly regarding pathogens with environmental niches, such as P. aeruginosa. It has been established that antibiotic concentrations below MICs can select antibiotic-resistant pathogens, a feature of relevance for analyzing the role of nonclinical ecosystems in antibiotic resistance evolution. The range of concentrations where this selection occurs defines the sub-MIC selective window, whose width depends on the antibiotic

Electronic Report Generation Web Service evaluated within a Telemedicine System

This work presents a generic tool based on a client-server architecture that generates electronic reports helping the evaluation process of any information system. For the specific evaluation of telemedicine systems the defined reports cover four dimensions: auditory of the system; evolution of clinical protocols; results from the questionnaires for user acceptance and quality of life; and surveillance of clinical variables. The use of a Web Service approach allows multiplatform use of the developed electronic report service and the modularity followed in the implementation enables easy system evolution and scalability

Mutational Evolution of Pseudomonas aeruginosa Resistance to Ribosome-Targeting Antibiotics

The present work examines the evolutionary trajectories of replicate Pseudomonas aeruginosa cultures in presence of the ribosome-targeting antibiotics tobramycin and tigecycline. It is known that large number of mutations across different genes – and therefore a large number of potential pathways – may be involved in resistance to any single antibiotic. Thus, evolution toward resistance might, to a large degree, rely on stochasticity, which might preclude the use of predictive strategies for fighting antibiotic resistance. However, the present results show that P. aeruginosa populations evolving in parallel in the presence of antibiotics (either tobramycin or tigecycline) follow a set of trajectories that present common elements. In addition, the pattern of resistance mutations involved include common elements for these two ribosome-targeting antimicrobials. This indicates that mutational evolution toward resistance (and perhaps other properties) is to a certain degree deterministic and, consequently, predictable. These findings are of interest, not just for P. aeruginosa, but in understanding the general rules involved in the evolution of antibiotic resistance also. In addition, the results indicate that bacteria can evolve toward higher levels of resistance to antibiotics against which they are considered to be intrinsically resistant, as tigecycline in the case of P. aeruginosa and that this may confer cross-resistance to other antibiotics of therapeutic value. Our results are particularly relevant in the case of patients under empiric treatment with tigecycline, which frequently suffer P. aeruginosa superinfections

Analysis of pesticide residues in honeybee (Apis mellifera L.) and in corbicular pollen. Exposure in citrus orchard with an integrated pest management system

In the last years, the honeybee population is facing growing threats such as expansion of pathogens, incorrect use of phytosanitary products and environmental contaminants, loss or fragmentation of habitat, invasive species and climate change. The citrus cropping by Integrated Pest Management (IPM) in Spain combines strategies to reduce pest populations preventing environmental problems and reducing levels of damage by using chemicals only when it is strictly necessary. The goal of this study is to develop a simple analytical method to evaluate pesticide residue levels in honeybees and corbicular pollen when honeybees are exposed to plant protection products (PPPs) used in integrated pest management citrus orchards. The proposed method is based in an ultrasound assisted extraction procedure followed by a dispersive solid phase extraction (d-SPE) clean-up with alumina and LC-MS/MS pesticides determination. The method was validated in samples of honeybee and corbicular pollen for 10 pesticides commonly used in citrus orchards under IPM. This procedure was compared with QuEChERS methodologies for these matrices. The developed method was applied to determine pesticides in both matrices in a two -year study in citrus orchards.info:eu-repo/semantics/acceptedVersio