Generación y caracterización casera de curvas de barrido en Z en muestras de betabel y jamaica

"El principal objetivo de este trabajo de tesis es obtener y estudiar curvas de barrido en Z, pero con arreglos caseros y deducir cuanto se aproximan estos resultados con los obtenidos en el laboratorio. Este trabajo se inició durante la pandemia y se continuó al regreso en la universidad, obteniendo así resultados experimentales con arreglos en el laboratorio y poder realizar una comparación entre éstos. La obtención de curvas experimentales de barrido en Z con un arreglo casero consistió en obtener estas curvas a través de imágenes capturadas con una cámara de celular, las cuales fueron procesadas con un programa en MATLAB para obtener un valor de la intensidad en cada posición de la muestra, alrededor de la cintura de un haz Gaussiano. En ambos casos se emplearon las mismas muestras, Betabel y flor de Jamaica, disueltos en alcohol. También se incluyó la caracterización del láser que se utilizó en los experimentos, para ver el tipo de haz que emite"

Correlación entre la estructura y dinámica familiar y el comportamiento de infracción de normas en adolescentes entre 12 y 16 años escolarizados en Bogotá.

Ante la impactante realidad colombiana relacionada con el creciente número de jóvenes infractores, cuyos reportes indican que cerca del 9,8 % de los delitos cometidos en el país, son realizados por menores de edad, con un promedio de 60 a 70 aprehensiones diarias desde 2011, con 42.357 capturados en 2014 y otros 7.300 hasta abril de 2015 y más de 500 pandillas juveniles de acuerdo a datos suministrados por el diario el Tiempo (13 de abril de 2015), P á g i n a 2 | 9 resulta urgente realizar estudios que ayuden a identificar los factores que inciden en el aumento de esta problemática para establecer medidas de mejoramiento. Por otro lado varios autores psicoanalistas (Proia N 2011, Rousillon R 1991, Flavigny 1977, Drieu D, 2010) anudan estos comportamientos infractores de normas con las nuevas formas de hacer familia de la actualidad (familias monoparentales, homoparentales, compuestas, simultaneas etc.)

Suppression of cell migration is promoted by miR-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells

Background: Aberrant expression of microRNAs has been associated with migration of tumor cells. In this study, we aimed to investigate the biological significance of miR-944 whose function is unknown in breast cancer. Methods: MiR-944 expression in breast cancer cells and tumors was evaluated by Taqman qRT-PCR assays. Transcriptional profiling of MDA-MB-231 cells expressing miR-944 was performed using DNA microarrays. Cell viability, migration and invasion were assessed by MTT, scratch/wound-healing and transwell chamber assays, respectively. The luciferase reporter assay was used to evaluate targeting of SIAH1, PTP4A1 and PRKCA genes by miR-944. SIAH1 protein levels were measured by Western blot. Silencing of SIAH1 gene was performed by RNA interference using shRNAs. Results: Our data showed that miR-944 expression was severely repressed in clinical specimens and breast cancer cell lines. Suppression of miR-944 levels was independent of hormonal status and metastatic potential of breast cancer cells. Gain-of-function analysis indicated that miR-944 altered the actin cytoskeleton dynamics and impaired cell migration and invasion. Genome-wide transcriptional profiling of MDA-MB-231 cells that ectopically express miR-944 showed that 15 genes involved in migration were significantly repressed. Notably, luciferase reporter assays confirmed the ability of miR-944 to bind the 3´UTR of SIAH1 and PTP4A1 genes, but not PRKCA gene. Congruently, an inverse correlation between miR-944 and SIAH1 protein expression was found in breast cancer cells. Moreover, SIAH1 was upregulated in 75 % of miR-944-deficient breast tumors. Finally, SIAH1 gene silencing by RNA interference significantly impaired cell migration of breast cancer cells. Conclusions: Our results pointed out that miR-944 is a novel upstream negative regulator of SIAH1 and PTP4A1 genes and provided for the first time evidence for its functional role in migration and invasion of breast cancer cells. They also suggest that miR-944 restoration may represent a potential strategy for breast cancer therapy

Tratado de justicia restaurativa. Un enfoque integrador

Esta obra nos presenta a la Justicia Restaurativa desde la configuración del derecho penal y los procesos de la misma en el orden penitenciario, en las comunidades escolares, en la igualdad de género. Realzando así mismo la habiliades del facilitador que habrá de llevarla a cabo, como parte integral de un nuevo paradigma en la formación de recursos humnos que atiendan la inminente necesidad de este tipo de ejercicio y en las redes primarias de apoyo, como elemento restaurativo para las mujeres víctimas de violencia doméstica, dónde ha crecido la respuesta de la sociedad para abatir este sector de la violenci

Implementation of a roadmap for the comprehensive diagnosis, follow-up, and research of childhood leukemias in vulnerable regions of Mexico: results from the PRONAII Strategy

The main objective of the National Project for Research and Incidence of Childhood Leukemias is to reduce early mortality rates for these neoplasms in the vulnerable regions of Mexico. This project was conducted in the states of Oaxaca, Puebla, and Tlaxcala. A key strategy of the project is the implementation of an effective roadmap to ensure that leukemia patients are the target of maximum benefit of interdisciplinary collaboration between researchers, clinicians, surveyors, and laboratories. This strategy guarantees the comprehensive management of diagnosis and follow-up samples of pediatric patients with leukemia, centralizing, managing, and analyzing the information collected. Additionally, it allows for a precise diagnosis and monitoring of the disease through immunophenotype and measurable residual disease (MRD) studies, enhancing research and supporting informed clinical decisions for the first time in these regions through a population-based study. This initiative has significantly improved the diagnostic capacity of leukemia in girls, boys, and adolescents in the regions of Oaxaca, Puebla, and Tlaxcala, providing comprehensive, high-quality care with full coverage in the region. Likewise, it has strengthened collaboration between health institutions, researchers, and professionals in the sector, which contributes to reducing the impact of the disease on the community

La investigación formativa en ciencias empresariales: .Experiencias de investigación formativa POLIPIF

El material propuesto en el libro resume gran parte de la calidad de los trabajos presentados y la evolución en el desarrollo de las capacidades de los estudiantes en un contexto actual, complejo y retador, que refleja la realidad de las organizaciones actuales sobre escenarios estratégicos y manejo de situaciones complejas, para que, cuando lleguen a ser empleados o emprendedores, sepan afrontar cada paso hacia el cambio

A novel scale based on biomarkers associated with COVID-19 severity can predict the need for hospitalization and intensive care, as well as enhanced probabilities for mortality

Abstract Prognostic scales may help to optimize the use of hospital resources, which may be of prime interest in the context of a fast spreading pandemics. Nonetheless, such tools are underdeveloped in the context of COVID-19. In the present article we asked whether accurate prognostic scales could be developed to optimize the use of hospital resources. We retrospectively studied 467 files of hospitalized patients after COVID-19. The odds ratios for 16 different biomarkers were calculated, those that were significantly associated were screened by a Pearson’s correlation, and such index was used to establish the mathematical function for each marker. The scales to predict the need for hospitalization, intensive-care requirement and mortality had enhanced sensitivities (0.91 CI 0.87–0.94; 0.96 CI 0.94–0.98; 0.96 CI 0.94–0.98; all with p < 0.0001) and specificities (0.74 CI 0.62–0.83; 0.92 CI 0.87–0.96 and 0.91 CI 0.86–0.94; all with p < 0.0001). Interestingly, when a different population was assayed, these parameters did not change considerably. These results show a novel approach to establish the mathematical function of a marker in the development of highly sensitive prognostic tools, which in this case, may aid in the optimization of hospital resources. An online version of the three algorithms can be found at: http://benepachuca.no-ip.org/covid/index.ph

Additional file 1: of Suppression of cell migration is promoted by miR-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells

Oligonucleotides sequences used to silencing SIAH1 gene expression. (PDF 6 kb

Diálogos y debates de la investigación jurídica y sociojurídica en Nariño

This book is divided into two thematic parts. The first addresses the most recent discussions regarding contemporary debates of law. Topics related to labor law —labor and sex workers rights— are discussed; private law —religious indoctrination—, the theory and philosophy of law —democracy, rule of law and responsibilities of the legislator—; and the relationship of law with education around peace and respect for diversity. The second part is devoted to Law and social struggles: private security policies and the monopoly of legitimate violence in the areas affected by the armed conflict; agrarian reform from a historical and economic perspective; the rights of nature; the role of ethnoeducation in the dynamics of empowerment of the indigenous and Afro-Colombian population; and the importance of sustainable development in the promotion and use of non-conventional renewable energy. The topics covered in are approached from economic, sociological, philosophical, and legal perspectives, which allows the debate about the realities of Nariñenses to be easily understood and analyzed for the different readers.Este libro está dividido en dos partes temáticas. La primera aborda las discusiones más recientes en torno a los debates contemporáneos del derecho. Se tocan temas relacionados con el derecho laboral —derechos laborales y de trabajadoras sexuales—; el derecho privado —adoctrinamiento religioso—, la teoría y filosofía del derecho —democracia, Estado de derecho y responsabilidades del legislador—; y la relación del derecho con la educación en torno a la paz y el respeto a la diversidad. La segunda parte está dedicada al derecho y las luchas sociales: las políticas de seguridad privada y el monopolio de la violencia legítima en las zonas afectadas por el conflicto armando; la reforma agraria desde una perspectiva histórica y económica; los derechos de la naturaleza; el papel de la etnoeducación en las dinámicas de empoderamiento de la población indígena y afrocolombiana; y la importancia del desarrollo sostenible en el fomento y uso de las energías renovables no convencionales. Los temas tratados en se abordan desde perspectivas económicas, sociológicas, filosóficas y jurídicas, lo que permite que el debate en torno a las realidades nariñenses resulte de fácil comprensión y análisis para los diferentes lectores.Palabras clave: comunidades afrocolombianas, comunidades indígenas, derecho al medio ambiente, derecho laboral, derechos de la naturaleza, etnoeducación, Nariño