Liquid chromatographic method for quantifying polyphenols in ciders by direct injection

An analytical method for the quantitative determination of the principal phenolic compounds (benzoic acids, hydroxycinnamic acids, 3-phenylpropionic acids, flavanols, procyanidins, dihydrochalcones, quercetin glycosides) in ciders, which successfully employs a RP-HPLC and photodiode-array detection system without prior treatment of the sample, is described. Parameters usually examined in the method validation were evaluated. Good linearity was obtained with correlation coefficients exceeding 0.999 and the detection limits ranged from 0.07 mg/L (p-hydroxybenzoic acid) to 2 mg/L (hydrocaffeic acid). Recoveries ranging between 90 and 104% and the reproducibility of the method was always <8% (RSD). The method was applied to a set of commercial samples and the results obtained may be helpful to establish a phenolic profile in Asturian cider

Efecto del aislamiento auditivo en la anorexia basada en la actividad

The objective was to study male and female rats exposed to Activity-Based Anorexia (ABA; simultaneous exposition to food restriction and free access to an activity wheel) under two conditions of sound attenuation, by means of different arrangements of wheels (standard versus isolation) in the laboratory room. Regardless of the sound attenuation condition, all but one male and one female with access to wheels had to be removed from the experiment, but extended ABA endurance was found in rats in the sound attenuation condition. Furthermore, significantly lower levels of running were observed in both males and females under the sound attenuation arrangement in the isolation condition. The results suggest that external stimulation plays a role in the activity displayed by rats exposed to ABA, and that the reduction of external stimulation diminishes running but does not protect rats from developing ABAEl objetivo fue el estudio de ratas macho y hembra expuestas al procedimiento experimental de Anorexia basada en la Actividad (ABA, exposición simultánea a restricción de alimento y acceso libre a una rueda de actividad) bajo dos condiciones de atenuación acústica por medio de un arreglo experimental diferente de las ruedas de actividad (estándar versus aislamiento) en el laboratorio. Independientemente de la condición de la atenuación del sonido, todos los animales con acceso a las ruedas de actividad menos una rata macho y otra hembra tuvieron que ser retirados del experimento, mientras que la condición de atenuación del sonido aumentó la resistencia a ABA. Además, los niveles de actividad de machos y hembras fueron significativamente inferiores en la condición de atenuación del sonido en la condición de aislamiento. Los resultados sugieren que la estimulación externa desempeña un papel en la actividad desplegada por las ratas expuestas a ABA, y que aunque la reducción de la estimulación externa disminuye la actividad no protege a los animales del desarrollo de ABAPreparation of manuscript and research reported was supported by grants from Xunta de Galicia, INCITE08PXIB211069PR, and the Ministerio de Educación y Ciencia de España, PSI2008-01081/ PSIC and by the research budget of the Venres Clinicos Unit, of the University of Santiago de CompostelaS

MERLIN: A new tool for flood hazard forecasting at the Galicia-Costa Hydrographic Demarcation

[EN] This article presents MERLIN, a tool for flood hazard evaluation, which forecasts discharges and water depths in flood prone areas of the Galicia Costa district. The warning system operates in two stages. During the hindcast stage, hydrological models of the basins included in the system assimilate hydro-meteorological data in order to characterize soil infiltration capacity. During the forecast stage, hydrological models are fed with meteorological predictions and discharge forecasts along the basins. Forecasted discharges define boundary conditions of hydraulic models, which compute the flood extent and the water depths over the upcoming days. The performance of MERLIN was evaluated in 4 areas using discharge data from the winter months of 2019-2020. Results proved MERLIN’s ability of predicting the discharges observed afterwards.[ES] Este artículo presenta MERLIN, una nueva herramienta para estimar el riesgo de inundaciones a partir de predicciones de caudales y calados en Áreas de Riesgo Potencial Significativo de Inundaciones (ARPSIS) de la demarcación hidrográfica Galicia-Costa. El sistema MERLIN opera en dos fases. Durante una primera fase de inicialización, modelos hidrológicos de las cuencas incluidas en el sistema asimilan datos hidro-meteorológicos para caracterizar la capacidad de infiltración del terreno. Durante la fase de predicción, los modelos hidrológicos previamente inicializados se alimentan con predicciones meteorológicas para determinar los caudales esperados durante los próximos días. Las predicciones de caudal alimentan a modelos hidráulicos de las ARPSIS que determinan los calados y la extensión de zonas inundadas. El funcionamiento de MERLIN se evaluó en 4 cuencas piloto a partir de los caudales registrados durante los temporales del invierno del 2019-2020, mostrando una buena capacidad de predecir los valores posteriormente observados.El desarrollo del sistema MERLIN y el resto de trabajos presentados en este artículo fue posible gracias a la financiación aportada por Augas de Galicia dentro del Convenio de colaboración entre Augas de Galicia e a Fundación de Enxeñería Civil de Galicia para a mellora do sistema de alerta temperá de risco de inundación na demarcación hidrográfica Galicia-costa.Fraga, I.; Cea, L.; Puertas, J.; Mosqueira, G.; Quinteiro, B.; Botana, S.; Fernández, L.... (2021). MERLIN: Una nueva herramienta para la predicción del riesgo de inundaciones en la demarcación hidrográfica Galicia-Costa. Ingeniería del agua. 25(3):215-227. https://doi.org/10.4995/ia.2021.15565OJS215227253Alvarez-Garreton, C., Ryu, D., Western, A.W., Su, C.H., Crow, W.T., Robertson, E., Leahy, C. 2015. Improving operational flood ensemble prediction by the assimilation of satellite soil moisture: Comparison between lumped and semi-distributed schemes. Hydrology and Earth System Sciences, 19(4), 1659-1676. https://doi.org/10.5194/hess-19-1659-2015Arnell, N.W., Gosling, S.N. 2016. The impacts of climate change on river flood risk at the global scale. Climatic Change, 134(3), 387-401. https://doi.org/10.1007/s10584-014-1084-5Bennett, T.H., Peters, J.C. 2000. Continuous soil moisture accounting in the hydrologic Engineering Center Hydrologic Modeling System (HEC-HMS). Building partnerships, 1-10.Berghuijs, W.R., Aalbers, E.E., Larsen, J.R., Trancoso, R., Woods, R.A. 2017. Recent changes in extreme floods across multiple continents. Environmental Research Letters, 12(11), 114035. https://doi.org/10.1088/1748-9326/aa8847Bladé, E., Cea, L., Corestein, G., Escolano, E., Puertas, J., Vázquez-Cendón, E., Dolz, J., Coll, A. 2014. Iber: herramienta de simulación numérica del flujo en ríos. Revista Internacional de Metodos Numericos en Ingeniería, 30(1), 1-10. https://doi.org/10.1016/j.rimni.2012.07.004Carracedo, P. 2003. Acoplamiento de un modelo hidrodinámico de escala global con uno de escala regional para Galicia. Revista Real Academia Galega de Ciencias, 22, 85.Cea, L., Fraga, I. 2018. Incorporating antecedent moisture conditions and intraevent variability of rainfall on flood frequency analysis in poorly gauged basins. Water Resources Research, 54, 8774-8791. https://doi.org/10.1029/2018WR023194Cronshey, R. 1986. Urban hydrology for small watersheds. US Department of Agriculture Soil Conservation Service Engineering Division.García-Feal, O., González-Cao, J., Gómez-Gesteira, M., Cea, L., Domínguez, J., Formella, A. 2018. An accelerated tool for flood modelling based on Iber. Water, 10(10) 1459. https://doi.org/10.3390/w10101459Hossain, F., Siddique-E-Akbor, A.H.M., Yigzaw, W., Shah-Newaz, S., Hossain, M., Mazumder, L.C., Turk, F.J. 2014. Crossing the "valley of death": lessons learned from implementing an operational satellite-based flood forecasting system. Bulletin of the American Meteorological Society, 95(8), 1201-1207. https://doi.org/10.1175/BAMS-D-13-00176.1IPCC (2018). Global warming of 1.5°C. An IPCC Special Report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas emission pathways in the context of strengthening the global response to the threat of climate change sustainable development and efforts to eradicate poverty. In Press.Jewell, S.A., Gaussiat, N. 2015. An assessment of kriging-based rain-gauge-radar merging techniques. Quarterly Journal of the Royal Meteorological Society, 141(691), 2300-2313. https://doi.org/10.1002/qj.2522Kasiviswanathan, K.S., He, J., Sudheer, K.P., Tay, J.H. 2016. Potential application of wavelet neural network ensemble to forecast streamflow for flood management. Journal of hydrology, 536, 161-173. https://doi.org/10.1016/j.jhydrol.2016.02.044Kellens, W., Vanneuville, W., Verfaillie, E., Meire, E., Deckers, P., De Maeyer, P. 2013. Flood risk management in Flanders: past developments and future challenges. Water Resources Management, 27(10), 3585-3606. https://doi.org/10.1007/s11269-013-0366-4Krajewski, W.F., Ceynar, D., Demir, I., Goska, R., Kruger, A., Langel, C., Small, S.J. 2017. Real-time flood forecasting and information system for the state of Iowa. Bulletin of the American Meteorological Society, 98(3), 539-554. https://doi.org/10.1175/BAMS-D-15-00243.1Kumar, M., Sahay, R.R. 2018. Wavelet-genetic programming conjunction model for flood forecasting in rivers. Hydrology Research, 49(6), 1880-1889. https://doi.org/10.2166/nh.2018.183Massari, C., Brocca, L., Tarpanelli, A., Moramarco, T. 2015. Data assimilation of satellite soil moisture into rainfall-runoff modelling: A complex recipe?. Remote Sensing, 7(9), 11403-11433. https://doi.org/10.3390/rs70911403McKay, M.D., Beckman, R.J., Conover, W.J. 1979 A Comparison of three methods for selecting values of input variables in the analysis of output from a computer code. Technometrics, 21(2), 239-245.Mure-Ravaud, M., Binet, G., Bracq, M., Perarnaud, J.J., Fradin, A., Litrico, X. 2016. A web based tool for operational realtime flood forecasting using data assimilation to update hydraulic states. Environmental Modelling and Software, 84, 35-49. https://doi.org/10.1016/j.envsoft.2016.06.002Naranjo, L., Taboada, J.J., Lage, A., Salsón, S., Montero, P., Souto, J.A., Pérez-Muñuzuri, V. 2001. Estudio de las anómalas condiciones meteorológicas sobre Galicia durante el otoño de los años 2000 y 2001. Revista Real Academia Galega de Ciencias, 20, 113-133Nguyen, P., Thorstensen, A., Sorooshian, S., Hsu, K., AghaKouchak, A., Sanders, B., Koren, V., Cui, Z., Smith, M. 2016. A high resolution coupled hydrologic-hydraulic model (HiResFlood-UCI) for flash flood modeling. Journal of Hydrology, 541, 401-420. https://doi.org/10.1016/j.jhydrol.2015.10.047Razmkhah, H. 2016. Comparing performance of different loss methods in rainfall-runoff modeling. Water resources, 43(1), 207-224. https://doi.org/10.1134/S0097807816120058Rosburg, T.T., Nelson, P.A., Bledsoe, B.P. 2017. Effects of urbanization on flow duration and stream flashiness: a case study of Puget Sound streams, western Washington, USA. Journal of the American Water Resources Association, 53(2), 493-507. https://doi.org/10.1111/1752-1688.12511Sanz-Ramos, M., Amengual, A., Bladé i Castellet, E., Romero, R., Roux, H. 2018. Flood forecasting using a coupled hydrological and hydraulic model (based on FVM) and highresolution meteorological model. Proceedings of River Flow 2018-Ninth International Conference on Fluvial Hydraulics (pp. 1-8) Lyon France. https://doi.org/10.1051/e3sconf/20184006028Scharffenberg, W.A, Fleming, M.J. 2006. Hydrologic modeling system HEC-HMS: User's manual. US Army Corps of Engineers Hydrologic Engineering Center.Shchepetkin, A.F., McWilliams, J.C. 2005. The regional oceanic modeling system (ROMS): a split-explicit free-surface topographyfollowing-coordinate oceanic model. Ocean Modelling, 9(4), 347-404. https://doi.org/10.1016/j.ocemod.2004.08.002Skamarock, W.C., Klemp, J.B., Dudhia, J., Gill, D.O., Barker, D.M., Wang, W., Powers, J.G. 2008. A description of the Advanced Research WRF version 3. NCAR Technical note-475+ STR.Sopelana, J., Cea, L., Ruano, S. 2018. A continuous simulation approach for the estimation of extreme flood inundation in coastal river reaches affected by meso and macro tides. Natural Hazards, 93(3) 1337-1358. https://doi.org/10.1007/s11069-018-3360-6Thielen, J., Bartholmes, J., Ramos, M. H., & Roo, A. D. 2009. The European flood alert system-part 1: concept and development. Hydrology and Earth System Sciences, 13(2), 125-140. https://doi.org/10.5194/hess-13-125-2009Thiemig, V., Bisselink, B., Pappenberger, F., Thielen, J. 2015. A pan-African medium-range ensemble flood forecast system. Hydrology and Earth System Sciences, 19(8), 3365-3385. https://doi.org/10.5194/hess-19-3365-2015U.S. Department of Agriculture, Natural Resources Conservation Service. 2010. National Engineering Handbook, Washington, DCVenâncio, A., Montero, P., Costa, P., Regueiro, S., Brands, S., Taboada, J. 2019. An Integrated Perspective of the Operational Forecasting System in Rías Baixas (Galicia, Spain) with Observational Data and End-Users. In International Conference on Computational Science (pp. 229-239). Springer, Cham. https://doi.org/10.1007/978-3-030-22747-0_18Wallemarq, P., Below, R., McLean, D. 2018. UNISDR and CRED report: Economic Losses, Poverty & Disasters (1998-2017).Wanders, N., Karssenberg, D., Roo, A.D., De Jong, S.M., Bierkens, M.F.P. 2014. The suitability of remotely sensed soil moisture for improving operational flood forecasting. Hydrology and Earth System Sciences, 18(6), 2343-2357. https://doi.org/10.5194/hess-18-2343-2014Weerts, A.H., Winsemius, H.C., Verkade, J.S. 2011. Estimation of predictive hydrological uncertainty using quantile regression: examples from the National Flood Forecasting System (England and Wales). Hydrology and Earth System Sciences, 15(1), 255-265. https://doi.org/10.5194/hess-15-255-2011Xia, X., Liang, Q., Ming, X. 2019. A full-scale fluvial flood modelling framework based on a high-performance integrated hydrodynamic modelling system (HiPIMS). Advances in Water Resources, 132, 103392. https://doi.org/10.1016/j.advwatres.2019.10339

CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease

Huntington’s disease (HD) is a hereditary neurodegenerative disorder of the basal ganglia for which disease-modifying treatments are not yet available. Although gene-silencing therapies are currently being tested, further molecular mechanisms must be explored to identify druggable targets for HD. Cytoplasmic polyadenylation element binding proteins 1 to 4 (CPEB1 to CPEB4) are RNA binding proteins that repress or activate translation of CPE-containing transcripts by shortening or elongating their poly(A) tail. Here, we found increased CPEB1 and decreased CPEB4 protein in the striatum of patients and mouse models with HD. This correlated with a reprogramming of polyadenylation in 17.3% of the transcriptome, markedly affecting neurodegeneration-associated genes including PSEN1, MAPT, SNCA, LRRK2, PINK1, DJ1, SOD1, TARDBP, FUS, and HTT and suggesting a new molecular mechanism in neurodegenerative disease etiology. We found decreased protein content of top deadenylated transcripts, including striatal atrophy–linked genes not previously related to HD, such as KTN1 and the easily druggable SLC19A3 (the ThTr2 thiamine transporter). Mutations in SLC19A3 cause biotin-thiamine–responsive basal ganglia disease (BTBGD), a striatal disorder that can be treated with a combination of biotin and thiamine. Similar to patients with BTBGD, patients with HD demonstrated decreased thiamine in the cerebrospinal fluid. Furthermore, patients and mice with HD showed decreased striatal concentrations of thiamine pyrophosphate (TPP), the metabolically active form of thiamine. High-dose biotin and thiamine treatment prevented TPP deficiency in HD mice and attenuated the radiological, neuropathological, and motor HD-like phenotypes, revealing an easily implementable therapy that might benefit patients with HD

Proceso asistencial integrado de esclerosis lateral amiotrófica

O proceso asistencial integrado da esclerose lateral amiotrófica, elaborouse co obxectivo de crear un proceso de traballo común en todas as áreas para facilitar a asistencia sanitaria ás persoas diagnosticadas desta enfermidade. Establécense actuacións como o asesoramento continuo, as consultas en acto único, a coordinación asistencial, tanto entre especialidades como coa atención primaria e a coordinación administrativa do sistema socio sanitario. Neste proceso participaron profesionais das diferentes áreas sanitarias especialistas en neuroloxía, endocrinoloxía, neumoloxía, psicoloxía clínica, rehabilitación, traballo social e hospitalización a domicilioEl proceso asistencial integrado de la esclerosis lateral amiotrófica, se elaboró con el objetivo de crear un proceso de trabajo común en todas las áreas para facilitar la asistencia sanitaria a las personas diagnosticadas de esta enfermedad. Se establecen actuaciones como el asesoramiento continuo, las consultas en acto único, la coordinación asistencial, tanto entre especialidades como con la atención primaria y la coordinación administrativa del sistema socio sanitario. En este proceso participaron profesionales de las diferentes áreas sanitarias especialistas en neurología, endocrinología, neumología, psicología clínica, rehabilitación, trabajo social y hospitalización a domicili

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

CIBERER: Spanish national network for research on rare diseases: A highly productive collaborative initiative

13 páginas,1 figura, 3 tablas, 1 apéndice. Se extraen los autores pertenecientes a The CIBERER network que trabajan en Centros del CSIC del Appendix ACIBER (Center for Biomedical Network Research; Centro de Investigación Biomédica En Red) is a public national consortium created in 2006 under the umbrella of the Spanish National Institute of Health Carlos III (ISCIII). This innovative research structure comprises 11 different specific areas dedicated to the main public health priorities in the National Health System. CIBERER, the thematic area of CIBER focused on rare diseases (RDs) currently consists of 75 research groups belonging to universities, research centers, and hospitals of the entire country. CIBERER's mission is to be a center prioritizing and favoring collaboration and cooperation between biomedical and clinical research groups, with special emphasis on the aspects of genetic, molecular, biochemical, and cellular research of RDs. This research is the basis for providing new tools for the diagnosis and therapy of low-prevalence diseases, in line with the International Rare Diseases Research Consortium (IRDiRC) objectives, thus favoring translational research between the scientific environment of the laboratory and the clinical setting of health centers. In this article, we intend to review CIBERER's 15-year journey and summarize the main results obtained in terms of internationalization, scientific production, contributions toward the discovery of new therapies and novel genes associated to diseases, cooperation with patients' associations and many other topics related to RD research.This study has been funded by Instituto de Salud Carlos III (ISCIII) and Spanish Ministry of Science and InnovationPeer reviewe

Early mobilisation in critically ill COVID-19 patients: a subanalysis of the ESICM-initiated UNITE-COVID observational study

Background Early mobilisation (EM) is an intervention that may improve the outcome of critically ill patients. There is limited data on EM in COVID-19 patients and its use during the first pandemic wave. Methods This is a pre-planned subanalysis of the ESICM UNITE-COVID, an international multicenter observational study involving critically ill COVID-19 patients in the ICU between February 15th and May 15th, 2020. We analysed variables associated with the initiation of EM (within 72 h of ICU admission) and explored the impact of EM on mortality, ICU and hospital length of stay, as well as discharge location. Statistical analyses were done using (generalised) linear mixed-effect models and ANOVAs. Results Mobilisation data from 4190 patients from 280 ICUs in 45 countries were analysed. 1114 (26.6%) of these patients received mobilisation within 72 h after ICU admission; 3076 (73.4%) did not. In our analysis of factors associated with EM, mechanical ventilation at admission (OR 0.29; 95% CI 0.25, 0.35; p = 0.001), higher age (OR 0.99; 95% CI 0.98, 1.00; p ≤ 0.001), pre-existing asthma (OR 0.84; 95% CI 0.73, 0.98; p = 0.028), and pre-existing kidney disease (OR 0.84; 95% CI 0.71, 0.99; p = 0.036) were negatively associated with the initiation of EM. EM was associated with a higher chance of being discharged home (OR 1.31; 95% CI 1.08, 1.58; p = 0.007) but was not associated with length of stay in ICU (adj. difference 0.91 days; 95% CI − 0.47, 1.37, p = 0.34) and hospital (adj. difference 1.4 days; 95% CI − 0.62, 2.35, p = 0.24) or mortality (OR 0.88; 95% CI 0.7, 1.09, p = 0.24) when adjusted for covariates. Conclusions Our findings demonstrate that a quarter of COVID-19 patients received EM. There was no association found between EM in COVID-19 patients' ICU and hospital length of stay or mortality. However, EM in COVID-19 patients was associated with increased odds of being discharged home rather than to a care facility. Trial registration ClinicalTrials.gov: NCT04836065 (retrospectively registered April 8th 2021)

Wine lactic acid bacteria with antimicrobial activity as potential biocontrol agents against fusarium oxysporum f. sp. lycopersici

Lactic acid bacteria isolated from wine fermentations, particularly from the malolactic fermentation, and belonging to Lactobacillus plantarum, Lactobacillus hilgardii, Lactobacillus paracasei and Lactococcus lactis species were tested for their effectiveness in inhibiting the development of different microorganisms. The different strains showed, to varying degrees, an antagonistic effect against bacteria of the genera Bacillus and Staphylococcus. The specificity of the species L. hilgardii that inhibits only strains of the genus Bacillus is remarkable, on the other hand, L. plantarum was more effective against the strains of the genus Staphylococcus. The greatest effectiveness, considering both the degree of inhibition and the number of inhibited species, was presented by strains of L. lactis and L. paracasei. Seven strains belonging to the species that showed a more broad-spectrum activity, L. paracasei and L. plantarum, were also tested for their ability to inhibit the growth of fungi. All of them showed, in different degree (55–76%), activity against Fusarium oxysporum. Finally, the ability of the L. paracasei LPAUV12 and L. plantarum LPLUV10 strains was evaluated to protect Lycopersicon esculentum plants against the fungus F. oxysporum and promote its growth. Strain LPLUV10, showed capacity to significantly inhibit the harmful effect of F. oxysporum in tomato plants as well as to significantly stimulate their growth.Universidade de Vigo | Ref. CN2012/108The Sustainable Use Network of Environmental and Agrifood Resource-REDUSO | Ref. CN2012/10

Wine Lactic Acid Bacteria with Antimicrobial Activity as Potential Biocontrol Agents against Fusarium oxysporum f. sp. lycopersici

Lactic acid bacteria isolated from wine fermentations, particularly from the malolactic fermentation, and belonging to Lactobacillus plantarum, Lactobacillus hilgardii, Lactobacillus paracasei and Lactococcus lactis species were tested for their effectiveness in inhibiting the development of different microorganisms. The different strains showed, to varying degrees, an antagonistic effect against bacteria of the genera Bacillus and Staphylococcus. The specificity of the species L. hilgardii that inhibits only strains of the genus Bacillus is remarkable, on the other hand, L. plantarum was more effective against the strains of the genus Staphylococcus. The greatest effectiveness, considering both the degree of inhibition and the number of inhibited species, was presented by strains of L. lactis and L. paracasei. Seven strains belonging to the species that showed a more broad-spectrum activity, L. paracasei and L. plantarum, were also tested for their ability to inhibit the growth of fungi. All of them showed, in different degree (55&ndash;76%), activity against Fusarium oxysporum. Finally, the ability of the L. paracasei LPAUV12 and L. plantarum LPLUV10 strains was evaluated to protect Lycopersicon esculentum plants against the fungus F. oxysporum and promote its growth. Strain LPLUV10, showed capacity to significantly inhibit the harmful effect of F. oxysporum in tomato plants as well as to significantly stimulate their growth