Use of video and 3D scenario visualisation to rate vegetation screens for integrating buildings into the landscape

Las acciones humanas en el medio natural crean un impacto visual. El objetivo principal de este estudio fue examinar la aceptación de pantallas de vegetación para integrar edificios en el paisaje utilizando nuevas técnicas de visualización en tres dimensiones (3D), videos y participación pública. El status quo de dos áreas de estudio representativas del clima mediterráneo en España (una tierra adentro y otra costera) se modeló, colocando pantallas de vegetación alrededor de un edificio típico de la zona y variando la densidad, las especies y el diseño de las plantas. Después de establecer una serie de escenarios, se crearon videos que muestran una simulación de actividades de turismo al aire libre realizadas a diferentes velocidades para analizar cómo el movimiento afecta la percepción de la combinación de un edificio y una pantalla de vegetación en las versiones creadas. Los modelos 3D generados fueron sometidos a una encuesta para la participación del público y para permitir que los encuestados califiquen sus preferencias de los videos creados. Los resultados muestran que agregar vegetación siempre mejora la integración de los edificios en el paisaje. La pantalla de vegetación con la calificación más alta colocada alrededor de los edificios en los dos escenarios fue la vegetación de alta densidad, independientemente de la distribución o la especie.Human actions in the natural environment create a visual impact. The main objective of this study was to examine the acceptance of vegetation screens for integrating buildings into the landscape using new techniques of visualization in three dimensions (3D), videos and public participation. The status quo of two study areas representative of the Mediterranean climate in Spain (one inland and one coastal) was modelled, placing vegetation screens around a building typical of the area and varying the density, species, and layout of plants. After establishing a series of scenarios, videos showing a simulation of outdoor tourism activities performed at varying speeds were created to analyse how movement affects the perception of the combination of a building and vegetation screen in the versions created. The 3D models generated were subjected to a survey for public participation and to allow respondents to rate their preferences of the videos created. The results show that adding vegetation always improves the integration of buildings into the landscape. The most highly rated vegetation screen placed around the buildings in the two scenarios was high density vegetation, irrespective of the layout or species.• Gobierno de Extremadura y Fondo Europeo Regional de Desarrollo. Ayuda GR15179peerReviewe

Guillain-Barré syndrome following the 2009 pandemic monovalent and seasonal trivalent influenza vaccination campaigns in Spain from 2009 to 2011: outcomes from active surveillance by a neurologist network, and records from a country-wide hospital discharge database

Background: Studies have shown a slight excess risk in Guillain-Barre syndrome (GBS) incidence associated with A(H1N1) pdm09 vaccination campaign and seasonal trivalent influenza vaccine immunisations in 2009-2010. We aimed to assess the incidence of GBS as a potential adverse effect of A(H1N1) pdm09 vaccination. Methods: A neurologist-led network, active at the neurology departments of ten general hospitals serving an adult population of 4.68 million, conducted GBS surveillance in Spain in 2009-2011. The network, established in 1996, carried out a retrospective and a prospective study to estimate monthly alarm thresholds in GBS incidence and tested them in 1998-1999 in a pilot study. Such incidence thresholds additionally to observation of GBS cases with immunisation antecedent in the 42 days prior to clinical onset were taken as alarm signals for 2009-2011, since November 2009 onwards. For purpose of surveillance, in 2009 we updated both the available centres and the populations served by the network. We also did a retrospective countrywide review of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis from January 2009 to December 2011. Results: Among 141 confirmed of 148 notified cases of GBS or Miller-Fisher syndrome, Brighton 1-2 criteria in 96 %, not a single patient was identified with clinical onset during the 42-day time interval following A(H1N1) pdm09 vaccination. In contrast, seven cases were seen during a similar period after seasonal campaigns. Monthly incidence figures did not, however, exceed the upper 95 % CI limit of expected incidence. A retrospective countrywide review of the registry of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis did not suggest higher admission rates in critical months across the period December 2009-February 2010. Conclusions: Despite limited power and underlying reporting bias in 2010-2011, an increase in GBS incidence over background GBS, associated with A(H1N1) pdm09 monovalent or trivalent influenza immunisations, appears unlikely

Spatio-temporal tumor heterogeneity in metastatic CRC tumors: a mutational-based approach

[EN] It is well known that activating mutations in the KRAS and NRAS genes are associated with poor response to anti-EGFR therapies in patients with metastatic colorectal cancer (mCRC). Approximately half of the patients with wild-type (WT) KRAS colorectal carcinoma do not respond to these therapies. This could be because the treatment decision is determined by the mutational profile of the primary tumor, regardless of the presence of small tumor subclones harboring RAS mutations in lymph nodes or liver metastases. We analyzed the mutational profile of the KRAS, NRAS, BRAF and PI3KCA genes using low-density microarray technology in samples of 26 paired primary tumors, 16 lymph nodes and 34 liver metastases from 26 untreated mCRC patients (n=76 samples). The most frequent mutations found in primary tumors were KRAS (15%) and PI3KCA (15%), followed by NRAS (8%) and BRAF (4%). The distribution of the mutations in the 16 lymph node metastases analyzed was as follows: 4 (25%) in KRAS gene, 3 (19%) in NRAS gene and 1 mutation each in PI3KCA and BRAF genes (6%). As expected, the most prevalent mutation in liver metastasis was in the KRAS gene (35%), followed by PI3KCA (9%) and BRAF (6%). Of the 26 cases studied, 15 (58%) displayed an overall concordance in the mutation status detected in the lymph node metastases and liver metastases compared with primary tumor, suggesting no clonal evolution. In contrast, the mutation profiles differed in the primary tumor and lymph node/metastases samples of the remaining 11 patients (48%), suggesting a spatial and temporal clonal evolution. We confirm the presence of different mutational profiles among primary tumors, lymph node metastases and liver metastases. Our results suggest the need to perform mutational analysis in all available tumor samples of patients before deciding to commence anti-EGFR treatment

Epidemiology of human papillomavirus-related oropharyngeal cancer in a classically low-burden region of southern Europe

The incidence of human papillomavirus (HPV)-related oropharyngeal cancer is increasing in some regions. Nevertheless, the epidemiology of this disease has not been extensively investigated in southern Europe. We conducted a retrospective cohort study of patients diagnosed with primary oropharyngeal cancer from 1991 to 2016. Cancer tissues underwent histopathological evaluation, DNA quality control, HPV-DNA detection and p16 immunohistochemistry. Data were collected from medical records. Factors associated with HPV positivity and time trends were evaluated with multivariable Bayesian models. The adjusted prevalence of HPV-related cases in 864 patients with a valid HPV-DNA result was 9.7%, with HPV-DNA/p16 double positivity being considered. HPV-related oropharyngeal cancer was likely to occur in non-smokers and non-drinkers, to be located in the tonsil or diagnosed at advanced stages. Time-trend analysis showed an increasing risk of HPV-related oropharyngeal cancer in the most recent periods (5-year period increase of 30%). This increase was highest and with a clear increasing trend only in the most recent years (2012-2016). The prevalence of HPV-related oropharyngeal cancer started to sharply increase in the most recent years in our setting, as occurred two decades ago in areas where most oropharyngeal cancer cases are currently HPV-related. Our results provide a comprehensive assessment of the epidemiological landscape of HPV-related oropharyngeal cancer in a region of southern Europe

Visual Analysis of the Height Ratio between Building and Background Vegetation. Two Rural Cases of Study: Spain and Sweden

The perception of apparent sizes of buildings in a rural environment depends on the height ratio between the building and its surrounding vegetation, and it is this parameter which is currently used to assess the built landscapes. The impact of a contrasting height is less strong if the building does not exceed the horizon line. For buildings overshooting the skyline, the building’s level of sharpness and number of lines in contrast to the sky determines the impact of the scales, and vegetation in the background helps to reduce impact. The specific objectives of the present study were: (1) finding height–ratio thresholds between building and background vegetation, which may improve the integration of rural buildings in sky-sensitive locations, and; (2) comparing the results in two rural contexts with very different climatic conditions: Spain and Sweden. A survey of eighteen scenarios (nine Spanish and nine Swedish), all digitally modified with different relative height ratios between vegetation and buildings, was performed. The survey was evaluated by the public from both countries. Regardless of the country of origin, integration of the building was good or very good when the vegetation in background did not exceed one half of the height of the construction. These results may be translated to technical criteria for planning assessment

Using Native Vegetation Screens to Lessen the Visual Impact of Rural Buildings in the Sierras de Béjar and Francia Biosphere Reserve: Case Studies and Public Survey

Tree screens have a demonstrated role in lessening the visual impact of buildings nonintegrated aesthetically by means of filtering façades. This is particularly useful on village fringes and in areas bordering urban green spaces. However, the role of other vegetal structure such as climber species, and their optimal percentage for façade filtering, have not been measured yet. The main objectives of present study were: (1) To guess if climber species have a similar positive role to lessen the visual impact of a façade than tree species, and (2) to compare optimal percentage of coverage for both vegetal structures. To explore them, we designed three percentages of partial-concealment vegetation screens (0% none, 40−50% medium, 70−80% high), comprising tree or climber native species from a study area, in eight buildings from the same region. As a result, 24 final infographics were evaluated by two groups of interviewees: 27 local people and 39 non-local university students. Respondents had to assess the integration of the building in terms of visual preference using an ascending scale with 5 options from “Very poor” = 1 to “Very good” = 5. The results show a clear linear positive response of participants when increasing the percentage of coverage by both types of vegetal screens. However, positive significant valuation over 3 points on average was reached before in tree species screens (3.06, in 40−50% of façade coverage) than in climbing species screens (3.02 in 70−80% of façade coverage). Finally, there was a high consensus in responses when both groups polled were compared

Visual Impact Assessment in Rural Areas: The Role of Vegetation Screening in the Sustainable Integration of Isolated Buildings

Rural tourism has led to an increase in the number of buildings, meaning that visual integration of these buildings into the landscape is not always achieved. The silhouettes of buildings in rural areas are always recognisably simple but can be visually discordant if their sharpness is high. The literature provides analyses of how the visual impact of a given construction can be minimised by vegetation screening. The main objective of this study was to propose a method of quantifying the visual impact of isolated buildings (1 (low visual impact)–5 (high visual impact)). The method combines a measurement of the sharpness of building silhouette lines and vegetation screening (Scr) percentage (high or low) using theories based on the cognitive aspects of visual perception and digital image processing. The method was validated through a survey in which photos were shown to a wide range of respondents. A second objective was to analyse the combined effect on the visual perception of Scr and building colour (C), which is broadly analysed in the literature. The main result is that the required percentage of vegetation screening for a building with sharp lines and discordant colours to be accepted was determined to be around 40%. The proposed method can be applied by landscape planners; it is easy to use, and the cognitive principles on which it is based do not depend on the working environment

Guillain-Barré syndrome following the 2009 pandemic monovalent and seasonal trivalent influenza vaccination campaigns in Spain from 2009 to 2011: outcomes from active surveillance by a neurologist network, and records from a country-wide hospital discharge database

BACKGROUND: Studies have shown a slight excess risk in Guillain-Barré syndrome (GBS) incidence associated with A(H1N1)pdm09 vaccination campaign and seasonal trivalent influenza vaccine immunisations in 2009-2010. We aimed to assess the incidence of GBS as a potential adverse effect of A(H1N1)pdm09 vaccination. METHODS: A neurologist-led network, active at the neurology departments of ten general hospitals serving an adult population of 4.68 million, conducted GBS surveillance in Spain in 2009-2011. The network, established in 1996, carried out a retrospective and a prospective study to estimate monthly alarm thresholds in GBS incidence and tested them in 1998-1999 in a pilot study. Such incidence thresholds additionally to observation of GBS cases with immunisation antecedent in the 42 days prior to clinical onset were taken as alarm signals for 2009-2011, since November 2009 onwards. For purpose of surveillance, in 2009 we updated both the available centres and the populations served by the network. We also did a retrospective countrywide review of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis from January 2009 to December 2011. RESULTS: Among 141 confirmed of 148 notified cases of GBS or Miller-Fisher syndrome, Brighton 1-2 criteria in 96 %, not a single patient was identified with clinical onset during the 42-day time interval following A(H1N1)pdm09 vaccination. In contrast, seven cases were seen during a similar period after seasonal campaigns. Monthly incidence figures did not, however, exceed the upper 95 % CI limit of expected incidence. A retrospective countrywide review of the registry of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis did not suggest higher admission rates in critical months across the period December 2009-February 2010. CONCLUSIONS: Despite limited power and underlying reporting bias in 2010-2011, an increase in GBS incidence over background GBS, associated with A(H1N1)pdm09 monovalent or trivalent influenza immunisations, appears unlikelyS

Guillain-Barré syndrome following the 2009 pandemic monovalent and seasonal trivalent influenza vaccination campaigns in Spain from 2009 to 2011: outcomes from active surveillance by a neurologist network, and records from a country-wide hospital discharge database

Background: Studies have shown a slight excess risk in Guillain-Barre syndrome (GBS) incidence associated with A(H1N1) pdm09 vaccination campaign and seasonal trivalent influenza vaccine immunisations in 2009-2010. We aimed to assess the incidence of GBS as a potential adverse effect of A(H1N1) pdm09 vaccination. Methods: A neurologist-led network, active at the neurology departments of ten general hospitals serving an adult population of 4.68 million, conducted GBS surveillance in Spain in 2009-2011. The network, established in 1996, carried out a retrospective and a prospective study to estimate monthly alarm thresholds in GBS incidence and tested them in 1998-1999 in a pilot study. Such incidence thresholds additionally to observation of GBS cases with immunisation antecedent in the 42 days prior to clinical onset were taken as alarm signals for 2009-2011, since November 2009 onwards. For purpose of surveillance, in 2009 we updated both the available centres and the populations served by the network. We also did a retrospective countrywide review of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis from January 2009 to December 2011. Results: Among 141 confirmed of 148 notified cases of GBS or Miller-Fisher syndrome, Brighton 1-2 criteria in 96 %, not a single patient was identified with clinical onset during the 42-day time interval following A(H1N1) pdm09 vaccination. In contrast, seven cases were seen during a similar period after seasonal campaigns. Monthly incidence figures did not, however, exceed the upper 95 % CI limit of expected incidence. A retrospective countrywide review of the registry of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis did not suggest higher admission rates in critical months across the period December 2009-February 2010. Conclusions: Despite limited power and underlying reporting bias in 2010-2011, an increase in GBS incidence over background GBS, associated with A(H1N1) pdm09 monovalent or trivalent influenza immunisations, appears unlikely

Guillain-Barré syndrome following the 2009 pandemic monovalent and seasonal trivalent influenza vaccination campaigns in Spain from 2009 to 2011: outcomes from active surveillance by a neurologist network, and records from a country-wide hospital discharge database

Background: Studies have shown a slight excess risk in Guillain-Barre syndrome (GBS) incidence associated with A(H1N1) pdm09 vaccination campaign and seasonal trivalent influenza vaccine immunisations in 2009-2010. We aimed to assess the incidence of GBS as a potential adverse effect of A(H1N1) pdm09 vaccination. Methods: A neurologist-led network, active at the neurology departments of ten general hospitals serving an adult population of 4.68 million, conducted GBS surveillance in Spain in 2009-2011. The network, established in 1996, carried out a retrospective and a prospective study to estimate monthly alarm thresholds in GBS incidence and tested them in 1998-1999 in a pilot study. Such incidence thresholds additionally to observation of GBS cases with immunisation antecedent in the 42 days prior to clinical onset were taken as alarm signals for 2009-2011, since November 2009 onwards. For purpose of surveillance, in 2009 we updated both the available centres and the populations served by the network. We also did a retrospective countrywide review of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis from January 2009 to December 2011. Results: Among 141 confirmed of 148 notified cases of GBS or Miller-Fisher syndrome, Brighton 1-2 criteria in 96 %, not a single patient was identified with clinical onset during the 42-day time interval following A(H1N1) pdm09 vaccination. In contrast, seven cases were seen during a similar period after seasonal campaigns. Monthly incidence figures did not, however, exceed the upper 95 % CI limit of expected incidence. A retrospective countrywide review of the registry of hospital-discharged patients having ICD-9-CM code 357.0 (acute infective polyneuritis) as their principal diagnosis did not suggest higher admission rates in critical months across the period December 2009-February 2010. Conclusions: Despite limited power and underlying reporting bias in 2010-2011, an increase in GBS incidence over background GBS, associated with A(H1N1) pdm09 monovalent or trivalent influenza immunisations, appears unlikely