The immunohistochemical expression of leptin in lymph node metastasis from laryngeal squamous cell carcinoma (SCC)

Introduction: Leptin is a proteohormone produced predominantly by white adipocytes and primarily known for its key role in the control of food intake and sense of satiety. From its discover leptin has been found in different body districts, involved in always new functions and processes. In the last years numerous relationships between leptin and cancer has been found. The aim of this study is to test the leptin positivity in human primitive laryngeal squamous cell carcinoma (SCC) and in its lymph node metastasis. Materials and methods: Leptin positivity was detected by immunohistochemical analysis on pathological samples from 18 patients subjected to laryngectomy and neck dissection for SCC. Results: During the study we pointed out a statistically significant relationship (p < 0.05) between leptin positivity levels and tumor differentiation grade, in particular we observed that a decrease in tumor leptin production correlates with higher level of cancer histological dedifferentiation. Conclusion: Our research on leptin expression in laryngeal squamous neoplastic pathology is aimed to the attempt of establishing a more precise patient risk stratification thanks to a new marker able to give a contribution to the identification of patient with poor prognosis and at risk of failure of actual standard therapy

An error estimate of Gaussian Recursive Filter in 3Dvar problem

Computational kernel of the three-dimensional variational data assimilation (3D-Var) problem is a linear system, generally solved by means of an iterative method. The most costly part of each iterative step is a matrix-vector product with a very large covariance matrix having Gaussian correlation structure. This operation may be interpreted as a Gaussian convolution, that is a very expensive numerical kernel. Recursive Filters (RFs) are a well known way to approximate the Gaussian convolution and are intensively applied in the meteorology, in the oceanography and in forecast models. In this paper, we deal with an oceanographic 3D-Var data assimilation scheme, named OceanVar, where the linear system is solved by using the Conjugate Gradient (GC) method by replacing, at each step, the Gaussian convolution with RFs. Here we give theoretical issues on the discrete convolution approximation with a first order (1st-RF) and a third order (3rd-RF) recursive filters. Numerical experiments confirm given error bounds and show the benefits, in terms of accuracy and performance, of the 3-rd RF.Comment: 9 page

Resting-state connectivity and functional specialization in human medial parieto-occipital cortex

According to recent models of visuo-spatial processing, the medial parieto-occipital cortex is a crucial node of the dorsal visual stream. Evidence from neurophysiological studies in monkeys has indicated that the parieto-occipital sulcus (POS) contains three functionally and cytoarchitectonically distinct areas: the visual area V6 in the fundus of the POS, and the visuo-motor areas V6Av and V6Ad in a progressively dorsal and anterior location with respect to V6. Besides different topographical organization, cytoarchitectonics, and functional properties, these three monkey areas can also be distinguished based on their patterns of cortico-cortical connections. Thanks to wide-field retinotopic mapping, areas V6 and V6Av have been also mapped in the human brain. Here, using a combined approach of resting-state functional connectivity and task-evoked activity by fMRI, we identified a new region in the anterior POS showing a pattern of functional properties and cortical connections that suggests a homology with the monkey area V6Ad. In addition, we observed distinct patterns of cortical connections associated with the human V6 and V6Av which are remarkably consistent with those showed by the anatomical tracing studies in the corresponding monkey areas. Consistent with recent models on visuo-spatial processing, our findings demonstrate a gradient of functional specialization and cortical connections within the human POS, with more posterior regions primarily dedicated to the analysis of visual attributes useful for spatial navigation and more anterior regions primarily dedicated to analyses of spatial information relevant for goal-directed action

A Lignin-Rich Extract of Giant Reed (Arundo donax L.) as a Possible Tool to Manage Soilborne Pathogens in Horticulture: A Preliminary Study on a Model Pathosystem

Finding new sustainable tools for crop protection in horticulture has become mandatory. Giant reed (Arundo donax L.) is a tall, perennial, widely diffuse lignocellulosic grass, mainly proposed for bioenergy production due to the fact of its high biomass yield and low agronomic requirements. Some studies have already highlighted antimicrobial and antifungal properties of giant reed-derived compounds. This study aimed at investigating the potential of a lignin-rich giant reed extract for crop protection. The extract, obtained by dry biomass treatment with potassium hydroxide at 120◦C, followed by neutralization, was chemically characterized. A preliminary in vitro screening among several pathogenic strains of fungi and oomycetes showed a high sensitivity by most of the soilborne pathogens to the extract; thus, an experiment was performed with the model pathosystem, Pythium ultimum–zucchini in a growth substrate composed of peat or sand. The adsorption by peat and sand of most of the lignin-derived compounds contained in the extract was also observed. The extract proved to be effective in restoring the number of healthy zucchini plantlets in the substrate infected with P. ultimum compared to the untreated control. This study highlights the potential of the lignin-rich giant reed extract to sustain crop health in horticulture

Age-related changes in ocular mucosal tolerance: Lessons learned from gut and respiratory tract immunity

The ocular surface is the part of the visual system directly exposed to the environment, and it comprises the cornea, the first refractive tissue layer and its surrounding structures. The ocular surface has evolved to keep the cornea smooth and wet, a prerequisite for proper sight, and also protected. To this aim, the ocular surface is a bona fide mucosal niche with an immune system capable of fighting against dangerous pathogens. However, due to the potential harmful effects of uncontrolled inflammation, the ocular surface has several mechanisms to keep the immune response in check. Specifically, the ocular surface is maintained inflammation-free and functional by a particular form of peripheral tolerance known as mucosal tolerance, markedly different from the immune privilege of intraocular structures. Remarkably, conjunctival tolerance is akin to the oral and respiratory tolerance mechanisms found in the gut and airways, respectively. And also similarly, this form of immunoregulation in the eye is affected by ageing just as it is in the digestive and respiratory tracts. With ageing comes an increased prevalence of immune-based ocular surface disorders, which could be related to an age-related impairment of conjunctival tolerance. The purpose of this review was to summarize the present knowledge of ocular mucosal tolerance and how it is affected by the ageing process in the light of the current literature on mucosal immunoregulation of the gut and airways.Fil: Galletti, Jeremías Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: de Paiva, Cintia S.. Baylor College of Medicine; Estados Unido

Importance of activity data for improving the residential wood combustion emission inventory at regional level

The contribution of residential wood combustion (RWC) to emission inventory at local level was estimated using a bottomeup approach for the Lombardy Region of North Italy. A survey, based on the CATI (Computer Assisted Telephone Interviewing) method, has been undertaken through 18,000 interviews. The interviews had the objective to characterize the RWC use in this region, in term of both total and municipal wood consumption. Details on the type of appliances used in RWC were also gathered. The results of the survey were then statistically analyzed in order to allow an estimate of RWC with high spatial resolution (i.e., at municipal level) in relation to the size and altitude of the territory. The work provides new evidence of the importance of wood combustion as a key source for PM and NMVOC emissions at local level, and thus highlights the importance of technological improvements and new policies aimed at emission reduction in this sector. Considering the great differences in average PM emission factors between low efficiency appliances (fireplaces, old stoves) and high efficiency ones (new stoves, pellet burners), this work emphasizes the importance of obtaining more detailed information on the types of wood appliances used for arriving at a reliable PM emission inventory for RWC

THE ROLE OF NOTCH PATHWAY IN MULTIPLE MYELOMA PROGRESSION: CONTROL OF IL-6 EXPRESSION IN MALIGNANT PLASMA CELLS AND BONE MARROW NICHE

Multiple myeloma (MM) is an incurable hematological tumor characterized by the accumulation of malignant plasma cells (PCs) within the bone marrow (BM). MM cells create a very strictly interaction with the surrounding BM niche that directly supports tumor growth through adhesion molecules and soluble factors, the most important of which is interleukin-6 (IL-6). IL-6 is probably the most important factor for MM cells growth and survival in vivo and it mainly produced by non-neoplastic cells of the BM microenvironment, and at less extent by malignant plasma cells. Recent studies suggest the involvement of NOTCH pathway in the pathogenesis of multiple myeloma. The NOTCH pathway is composed by four isoforms of receptors (NOTCH1-4) and two family of ligands, the Serrate-like ligands (JAGGED1 and 2) and the Delta-like ligands (DLL1/3/4). NOTCH pathway plays an important role in several cellular processes such as proliferation, survival, differentiation and stemness in various tissues and tumors. Regarding MM, the up-regulation of NOTCH signaling is due to the over-expression of both receptors (NOTCH1 and NOTCH2) and ligands (JAGGED1 and JAGGED2): these alterations promote tumor growth, pharmacological resistance, and bone disease. Furthermore, NOTCH pathway plays an important role in the pathological interaction between malignant PCs and the BM niche. This study indicates that there is a correlation between levels of NOTCH signaling members (JAGGED1, NOTCH2, HES5 and HES6) and the malignant progression of the disease. Indeed, NOTCH pathway is involved in multiple myeloma progression since it is able to increase the release of IL-6 produced by BM stromal cells (BMSCs). Our in vitro studies show that NOTCH pathway induces IL-6 gene expression in MM cells as well as in the surrounding BMSCs through the over-expression of JAGGED ligands. The activation of NOTCH pathway in the BMSCs renders these cells able to support tumor cells growth through the production of IL-6. These data are confirmed also by correlation analysis on gene expression profiles of MM patients and immunohistochemical studies. The ability of NOTCH pathway to positively regulate IL-6 in the non-neoplastic cells of BM microenvironment renders this pathway an important mediator of tumor-directed reprogramming of bone marrow niche. These results support the rationale for a NOTCH-direct therapeutic approach that can specifically target JAGGED ligands in order to reduce side effects associated with common NOTCH signaling inhibitor

A non-linear observer for unsteady three-dimensional flows

A method is proposed to estimate the velocity field of an unsteady flow using a limited number of flow measurements. The method is based on a non-linear low-dimensional model of the flow and on expanding the velocity field in terms of empirical basis functions. The main idea is to impose that the coefficients of the modal expansion of the velocity field give the best approximation to the available measurements and that at the same time they satisfy as close as possible the non-linear low-order model. The practical use may range from feedback flow control to monitoring of the flow in non-accessible regions. The proposed technique is applied to the flow around a confined square cylinder, both in two- and three-dimensional laminar flow regimes. Comparisons are provided. with existing linear and non-linear estimation techniques