Power and politics in research design and practice: Opening up space for social equity in interdisciplinary, multi-jurisdictional and community-based research

Working collaboratively with communities is commonly considered a cornerstone of good practice in research involving social-ecological concerns. Increasingly, funding agencies also recognise that such collaborations are most productive when community partners have some influence on the design and implementation of the projects that benefit from their participation. However, researchers engaged with this work often struggle to actively engage community members in this way and, in particular, Indigenous peoples. In this article, we argue that useful strategies for facilitating such engagement are to leave space in the research plan for questions of interest to community partners and to encourage equitable interactions between all participants through the use of forums in which power dynamics are intentionally flattened. We demonstrate the use of this technique in an interdisciplinary, multi-jurisdictional research study involving the fate and transport of toxic compounds that lead to fish consumption advisories throughout the world. In this project, the use of participatory forums resulted in community partners in Michigan’s Keweenaw Bay area of Lake Superior shaping a key aspect of the research by raising the simple but significant question: ‘When can we eat the fish?’. Their interest in this question also helped to ensure that they would remain meaningful partners throughout the duration of the project. The conclusion emphasises that further integration of Indigenous and community-based research methods has the potential to significantly enhance the process and value of university-community research engagement in the future

Power and politics in research design and practice: Opening up space for social equity in interdisciplinary, multi-jurisdictional and community-based research

Working collaboratively with communities is commonly considered a cornerstone of good practice in research involving social-ecological concerns. Increasingly, funding agencies also recognise that such collaborations are most productive when community partners have some influence on the design and implementation of the projects that benefit from their participation. However, researchers engaged with this work often struggle to actively engage community members in this way and, in particular, Indigenous peoples. In this article, we argue that useful strategies for facilitating such engagement are to leave space in the research plan for questions of interest to community partners and to encourage equitable interactions between all participants through the use of forums in which power dynamics are intentionally flattened. We demonstrate the use of this technique in an interdisciplinary, multi-jurisdictional research study involving the fate and transport of toxic compounds that lead to fish consumption advisories throughout the world. In this project, the use of participatory forums resulted in community partners in Michigan’s Keweenaw Bay area of Lake Superior shaping a key aspect of the research by raising the simple but significant question: ‘When can we eat the fish?’. Their interest in this question also helped to ensure that they would remain meaningful partners throughout the duration of the project. The conclusion emphasises that further integration of Indigenous and community-based research methods has the potential to significantly enhance the process and value of university-community research engagement in the future

François Caron et la permanence des représentations françaises du Japon entre le XVIe et le XVIIIe siècle

À partir de 1635, le Japon de la dynastie des Tokugawa se replie sur lui-même en officialisant sa fermeture aux échanges avec l’étranger, avec la mise en place de la politique isolationniste du sakoku, et seuls les marchands opérant pour le compte de la Compagnie néerlandaise des Indes orientales sont désormais autorisés à commercer au pays, de manière limitée. C’est dans ce contexte que fut rédigée, en 1636, la Vraie description du puissant royaume du Japon, par un marchand hollandais protestant du nom de François Caron. L’ouvrage, un bref document administratif, qui n’était d’abord pas destiné à être publié, connût néanmoins un vif succès à partir de 1661, et fut traduit dans plusieurs langues. Dans le cadre de ce mémoire, nous étudierons l’influence de la Vraie description sur les représentations des Japonais dans la France du XVIIe siècle, à travers les riches descriptions faites par son auteur de la société et de la culture du Japon de l’ère Edo. Pour ce faire, nous allons dans un premier temps analyser les écrits des Jésuites, qui sont les premiers à construire une image particulière des Japonais et en influencer durablement les représentations. Dans un second temps, nous analyserons en détail la Vraie description pour en dégager l’image qu’a pu avoir un marchand hollandais du Japon du XVIIe siècle. Dans un troisième et dernier temps, nous nous intéresserons à l’influence de la Vraie description sur la manière dont les auteurs français écrivirent sur le Japon à la fin du XVIIe siècle et au début de l’époque des Lumières.From 1635 onward, Tokugawa Japan closed itself to any type of foreign trade, with the establishment of the isolationist policy of sakoku, and only merchants operating on behalf of the Dutch East India Company were permitted to trade in the country, on a limited basis. It was in this context that the True Description of the Mighty Kingdoms of Japan and Siam was written, in 1636, by a Protestant Dutch merchant known as François Caron. The work, a brief administrative document, which was not initially intended for publication, was a great success from its first publication in 1661, and was translated into several languages. As part of this thesis, we will study the impact of the True Description on perceptions of the Japanese in 17th century France, through the rich descriptions made by its author of the society and culture of Edo Japan. To do this, we will first analyze the writings of the Jesuits, who were the first to build a particular image of the Japanese and to have a lasting influence on the way they were described and perceived. Secondly, we will analyze in detail the content of the True Description in order to determine the image that a Dutch merchant of the 17th century may have had of Japan and its people. As a conclusion, we will examine the influence of the True Description on the way French authors wrote about Japan from the end of the 17th century to the beginning of the Enlightenment

Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing

Skeletal muscle (SM) acts as a secretory organ, capable of releasing myokines and extracellular vesicles (SM‐EVs) that impact myogenesis and homeostasis. While age‐related changes have been previously reported in murine SM‐EVs, no study has comprehensively profiled SM‐EV in human models. To this end, we provide the first comprehensive comparison of SM‐EVs from young and old human primary skeletal muscle cells (HPMCs) to map changes associated with SM ageing. HPMCs, isolated from young (24 ± 1.7 years old) and older (69 ± 2.6 years old) participants, were immunomagnetically sorted based on the presence of the myogenic marker CD56 (N‐CAM) and cultured as pure (100% CD56+) or mixed populations (MP: 90% CD56+). SM‐EVs were isolated using an optimised protocol combining ultrafiltration and size exclusion chromatography (UF + SEC) and their biological content was extensively characterised using Raman spectroscopy (RS) and liquid chromatography mass spectrometry (LC‐MS). Minimal variations in basic EV parameters (particle number, size, protein markers) were observed between young and old populations. However, biochemical fingerprinting by RS highlighted increased protein (amide I), lipid (phospholipids and phosphatidylcholine) and hypoxanthine signatures for older SM‐EVs. Through LC‐MS, we identified 84 shared proteins with functions principally related to cell homeostasis, muscle maintenance and transcriptional regulation. Significantly, SM‐EVs from older participants were comparatively enriched in proteins involved in oxidative stress and DNA/RNA mutagenesis, such as E3 ubiquitin‐protein ligase TTC3 (TTC3), little elongation complex subunit 1 (ICE1) and Acetyl‐CoA carboxylase 1 (ACACA). These data suggest SM‐EVs could provide an alternative pathway for homeostasis and detoxification during SM ageing

Expansion microscopy of zebrafish for neuroscience and developmental biology studies

Expansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility of ExM in the larval and embryonic zebrafish, an important model organism for the study of neuroscience and development. Regarding neuroscience, we found that ExM enabled the tracing of fine processes of radial glia, which are not resolvable with diffraction-limited microscopy. ExM further resolved putative synaptic connections, as well as molecular differences between densely packed synapses. Finally, ExM could resolve subsynaptic protein organization, such as ring-like structures composed of glycine receptors. Regarding development, we used ExM to characterize the shapes of nuclear invaginations and channels, and to visualize cytoskeletal proteins nearby. We detected nuclear invagination channels at late prophase and telophase, potentially suggesting roles for such channels in cell division. Thus, ExM of the larval and embryonic zebrafish may enable systematic studies of how molecular components are configured in multiple contexts of interest to neuroscience and developmental biology.National Institutes of Health (U.S.) (Grant 1R01EB024261)National Institutes of Health (U.S.) (Grant 1R01MH110932)National Institutes of Health (U.S.) (Grant 2R01DA029639)National Institutes of Health (U.S.) (Grant 1R01NS087950)National Institutes of Health (U.S.) (Grant 1U01MH106011

Flower-visitor communities of an arcto-alpine plant-Global patterns in species richness, phylogenetic diversity and ecological functioning

Pollination is an ecosystem function of global importance. Yet, who visits the flower of specific plants, how the composition of these visitors varies in space and time and how such variation translates into pollination services are hard to establish. The use of DNA barcodes allows us to address ecological patterns involving thousands of taxa that are difficult to identify. To clarify the regional variation in the visitor community of a widespread flower resource, we compared the composition of the arthropod community visiting species in the genus Dryas (mountain avens, family Rosaceae), throughout Arctic and high-alpine areas. At each of 15 sites, we sampled Dryas visitors with 100 sticky flower mimics and identified specimens to Barcode Index Numbers (BINs) using a partial sequence of the mitochondrial COI gene. As a measure of ecosystem functioning, we quantified variation in the seed set of Dryas. To test for an association between phylogenetic and functional diversity, we characterized the structure of local visitor communities with both taxonomic and phylogenetic descriptors. In total, we detected 1,360 different BINs, dominated by Diptera and Hymenoptera. The richness of visitors at each site appeared to be driven by local temperature and precipitation. Phylogeographic structure seemed reflective of geological history and mirrored trans-Arctic patterns detected in plants. Seed set success varied widely among sites, with little variation attributable to pollinator species richness. This pattern suggests idiosyncratic associations, with function dominated by few and potentially different taxa at each site. Taken together, our findings illustrate the role of post-glacial history in the assembly of flower-visitor communities in the Arctic and offer insights for understanding how diversity translates into ecosystem functioning.Peer reviewe

Clinical risk score for persistent postconcussion symptomsamong children with acute concussion in the ED

IMPORTANCE Approximately one-third of children experiencing acute concussion experience ongoing somatic, cognitive, and psychological or behavioral symptoms, referred to as persistent postconcussion symptoms (PPCS). However, validated and pragmatic tools enabling clinicians to identify patients at risk for PPCS do not exist. OBJECTIVE To derive and validate a clinical risk score for PPCS among children presenting to the emergency department. DESIGN, SETTING, AND PARTICIPANTS Prospective, multicenter cohort study (Predicting and Preventing Postconcussive Problems in Pediatrics [5P]) enrolled young patients (aged 5-\u3c18 years) who presented within 48 hours of an acute head injury at 1 of 9 pediatric emergency departments within the Pediatric Emergency Research Canada (PERC) network from August 2013 through September 2014 (derivation cohort) and from October 2014 through June 2015 (validation cohort). Participants completed follow-up 28 days after the injury. EXPOSURES All eligible patients had concussions consistent with the Zurich consensus diagnostic criteria. MAIN OUTCOMES AND MEASURES The primary outcomewas PPCS risk score at 28 days, which was defined as 3 or more new or worsening symptoms using the patient-reported Postconcussion Symptom Inventory compared with recalled state of being prior to the injury. RESULTS In total, 3063 patients (median age, 12.0 years [interquartile range, 9.2-14.6 years]; 1205 [39.3%] girls) were enrolled (n = 2006 in the derivation cohort; n = 1057 in the validation cohort) and 2584 of whom (n = 1701 [85%] in the derivation cohort; n = 883 [84%] in the validation cohort) completed follow-up at 28 days after the injury. Persistent postconcussion symptoms were present in 801 patients (31.0%) (n = 510 [30.0%] in the derivation cohort and n = 291 [33.0%] in the validation cohort). The 12-point PPCS risk score model for the derivation cohort included the variables of female sex, age of 13 years or older, physician-diagnosed migraine history, prior concussion with symptoms lasting longer than 1 week, headache, sensitivity to noise, fatigue, answering questions slowly, and 4 or more errors on the Balance Error Scoring System tandem stance. The area under the curve was 0.71 (95%CI, 0.69-0.74) for the derivation cohort and 0.68 (95%CI, 0.65-0.72) for the validation cohort. CONCLUSIONS AND RELEVANCE A clinical risk score developed among children presenting to the emergency department with concussion and head injury within the previous 48 hours had modest discrimination to stratify PPCS risk at 28 days. Before this score is adopted in clinical practice, further research is needed for external validation, assessment of accuracy in an office setting, and determination of clinical utility

Habitat features and their influence on the restoration potential of marine habitats in Europe

To understand the restoration potential of degraded habitats, it is important to know the key processes and habitat features that allow for recovery after disturbance. As part of the EU (Horizon 2020) funded MERCES project, a group of European experts compiled and assessed current knowledge, from both past and ongoing restoration efforts, within the Mediterranean Sea, the Baltic Sea, and the North-East Atlantic Ocean. The aim was to provide an expert judgment of how different habitat features could impact restoration success and enhance the recovery of marine habitats. A set of biological and ecological features (i.e., life-history traits, population connectivity, spatial distribution, structural complexity, and the potential for regime shifts) were identified and scored according to their contribution to the successful accomplishment of habitat restoration for five habitats: seagrass meadows, kelp forests, Cystoseira macroalgal beds, coralligenous assemblages and cold-water coral habitats. The expert group concluded that most of the kelp forests features facilitate successful restoration, while the features for the coralligenous assemblages and the cold-water coral habitat did not promote successful restoration. For the other habitats the conclusions were much more variable. The lack of knowledge on the relationship between acting pressures and resulting changes in the ecological state of habitats is a major challenge for implementing restoration actions. This paper provides an overview of essential features that can affect restoration success in marine habitats of key importance for valuable ecosystem services

State of the knowledge on European marine habitat mapping and degraded habitats

During the last decades, several EU Directives and other international legislations have generated a large number of national initiatives (e.g. marine atlases) and EU programmes on habitat mapping. Nevertheless, the outcomes of these initiatives are fragmented and, to our best knowledge, to date there is no systematic assessment regarding the nature, quality and availability of information across the European seas. One of the main goals of the MERCES project (www.merces-project.eu) is to produce a census of available maps of European key marine habitats, along with their degradation status and restoration potential in the European Seas, providing a potential basis for future discussion on restoration activities. MERCES is producing a census of European marine key habitat maps, degraded habitat maps and investigating key habitat restoration potential. To do this MERCES has i. reviewed known existing habitat maps of European regional seas and provided source citations for all of the information ii. reviewed degraded habitat map resources by regional sea and habitat type (e.g. seagrass, macroalgae, coral gardens, sponge aggregations, seamounts, vents), associated habitat deterioration (e.g. extent of decline), the most common human activities and pressures reported, and the recovery and restoration potential of these habitats iii. reviewed 6 key habitats (including kelp and macroalgal forests, seagrass meadows, coralligenous assemblages, coral gardens and deep-sea bottom communities) and linked 6 major habitat features, such as dynamics, connectivity, structural complexity and vulnerability, to consequences for restoration and the likelihood of restoration succes

Current marine pressures and mechanisms driving changes in marine habitats

Human activities and the resultant pressures they place on the marine environment have been widely demonstrated to contribute to habitat degradation, therefore, their identification and quantification is an essential step towards any meaningful restoration effort. The overall scope of MERCES Deliverable 1.2 is to review current knowledge regarding the major marine pressures placed upon marine ecosystems in EU waters and the mechanisms by which they impact habitats in order to determine potential restoration pathways. An understanding of their geographical distribution is critical for any local assessment of degradation, as well as for planning conservation and restoration actions. This information would ideally be in the form of maps, which: (a) compile single or multiple activities and pressures over broad scales, integrating and visualizing available data and allowing direct identification of aggregations as well as gaps and (b) may be overlaid with habitat maps (or any other map layer containing additional information), thus combining different data levels and producing new information to be used for example when implementing EU policies. The deliverable also documents typical example habitat case studies, the prominent impacts and consequences of activities and pressures towards the identification of possible restoration or mitigation actions. Finally the deliverable discusses pressures, assessments, marine spatial planning and blue growth potential. Activities and pressures are used in a strict sense, where marine activities are undertaken to satisfy the needs of societal drivers (e.g. aquaculture or tourism) and pressures are considered to be the mechanism through which an activity has an actual or potential effect on any part of the ecosystem (e.g. for demersal trawling activity, one pressure would be abrasion of the seabed). Habitats are addressed using a nested approach from large-scale geological features (e.g. shallow soft bottoms) to species-characterised habitats (e.g. Posidonia meadows) because of the way they are referred to in current policy documents which lack standard and precise definitions