Paternal exposure to benzo(a)pyrene induces genome-wide mutations in mouse offspring

Understanding the effects of environmental exposures on germline mutation rates has been a decades-long pursuit in genetics. We used next-generation sequencing and comparative genomic hybridization arrays to investigate genome-wide mutations in the offspring of male mice exposed to benzo(a)pyrene (BaP), a common environmental pollutant. We demonstrate that offspring developing from sperm exposed during the mitotic or post-mitotic phases of spermatogenesis have significantly more de novo single nucleotide variants (1.8-fold; P < 0.01) than controls. Both phases of spermatogenesis are susceptible to the induction of heritable mutations, although mutations arising from post-fertilization events are more common after post-mitotic exposure. In addition, the mutation spectra in sperm and offspring of BaP-exposed males are consistent. Finally, we report a significant increase in transmitted copy number duplications (P = 0.001) in BaP-exposed sires. Our study demonstrates that germ cell mutagen exposures induce genome-wide mutations in the offspring that may be associated with adverse health outcomes

The links between self-determined motivations and behavioral automaticity in a variety of real-life behaviors

The different motivations postulated by the Self-Determination Theory have proved to be meaningful to predict the level of engagement in a wide variety of life domains. The present research examines the relation between the different forms of self-determined motivation and behavioral automaticity of 12 behaviors associated with different life domains. Following 1743 measurements of self-determination (using a short version of the situational motivational scale, SIMS8), behavioral automaticity (using the self-reported behavioral automaticity index, SRBAI), and behavioral frequency (self-reported number of executions in a unit of time) for 12 various common behaviors collected on 315 young adults ( Mage = 20.60 ± 2.87 years) through an online survey, the results of crossed linear mixed models indicated that self-determined motivations are more associated with behavioral automaticity than nonself- determined motivations (intrinsic motivation: β = 0.13, p < .001, identified extrinsic motivation: β = 0.13, p < .001; external extrinsic motivation : β = 0.08, p < .001; amotivation: β = 0.02, p = .433). Furthermore, self-determination played a moderating role between the repetition of behaviors and behavioral automaticity (β = 0.06, p < .002) suggesting that self-determination facilitated automatization,as high level of behavioral automaticity was achieved with less frequent behaviors when behaviors were performed for highly self-determined (β = 0.41, p < .001) than weakly self-determined reasons (β = 0.29, p < .001). The applications of these findings for learning and habit formation are discussed

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009–31 January 2010

This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus